Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc10k16
(801 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF... 126 3e-30
SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F ... 123 3e-29
SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa... 29 1.0
SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 26 5.4
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 26 7.2
SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 26 7.2
SPAC1805.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 7.2
>SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF4E
4F complex subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 243
Score = 126 bits (305), Expect = 3e-30
Identities = 70/188 (37%), Positives = 101/188 (53%), Gaps = 4/188 (2%)
Frame = +2
Query: 104 NAEVPPEFLIKHPLQNQWSLWFYDND-RNKTWEENLIELTTFDTVEDFWRLYHHIKLPSE 280
NAE F+ HPLQ++W+LWF + W + L E+ +F TVE+FW ++ I S
Sbjct: 55 NAETA--FVKTHPLQHEWTLWFLKPPTQGLEWSDLLKEIISFKTVEEFWGIFKTISKASM 112
Query: 281 LRQGHDYAVFKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFE- 457
L DY+ F +GIRP WED N GG+W + + K + ++LD WL +VL IGE +
Sbjct: 113 LPAKSDYSYFLKGIRPEWEDPQNMNGGKW--AYQSKHKGSNLDELWLYMVLAAIGETLDP 170
Query: 458 NSDEICGAVVNVRPKVDKIAIWTADAMKQHATIEIGKKLKEQLGIHGK--IGFQVHRDTM 631
E+ G V N+R +IA+WT + + +IG + KE LGI K I + H D+
Sbjct: 171 TGKEVTGVVCNMRKGFYRIAVWTRNCNDKDVLEKIGLRFKEVLGISDKETIEYSAHEDSS 230
Query: 632 VKHSSATK 655
S K
Sbjct: 231 KAGSMRAK 238
>SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F
complex subunit |Schizosaccharomyces pombe|chr
1|||Manual
Length = 218
Score = 123 bits (297), Expect = 3e-29
Identities = 64/186 (34%), Positives = 95/186 (51%), Gaps = 4/186 (2%)
Frame = +2
Query: 125 FLIKHPLQNQWSLWFY-DNDRNKTWEENLIELTTFDTVEDFWRLYHHIKLPSELRQGHDY 301
F +KHPL W+LWF W E + TF++VE+FW ++++I S L DY
Sbjct: 34 FNLKHPLARPWTLWFLMPPTPGLEWNELQKNIITFNSVEEFWGIHNNINPASSLPIKSDY 93
Query: 302 AVFKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFE-NSDEICG 478
+ F++G+RP WED NK GG+W K + LD WL VL IGE + E+ G
Sbjct: 94 SFFREGVRPEWEDVHNKTGGKWAFQ-NKGRGGNALDEMWLTTVLAAIGETLDPTGQEVMG 152
Query: 479 AVVNVRPKVDKIAIWTADAMKQHATIEIGKKLKEQLGI--HGKIGFQVHRDTMVKHSSAT 652
V+N+R ++A+WT + +EIG + K+ L + I F H D+ S+
Sbjct: 153 VVINMRKGFYRLAVWTKSCNNREVLMEIGTRFKQVLNLPRSETIEFSAHEDSSKSGSTRA 212
Query: 653 KNLYTV 670
K +V
Sbjct: 213 KTRMSV 218
>SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 783
Score = 28.7 bits (61), Expect = 1.0
Identities = 16/34 (47%), Positives = 21/34 (61%), Gaps = 2/34 (5%)
Frame = +1
Query: 589 DTRQDW-FPSTQRH-NG*A*FCDQESIHCLVLAL 684
D Q+W FP TQ++ N FC+Q H L+LAL
Sbjct: 98 DAMQNWIFPQTQQYRNYQKEFCEQALFHNLLLAL 131
>SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 3971
Score = 26.2 bits (55), Expect = 5.4
Identities = 27/99 (27%), Positives = 46/99 (46%)
Frame = -1
Query: 483 TAPQISSEFSKFSPIKRRTTSNQNLSKSVKRCFFSRLISHLPPILLASSSHIGRMPCLNT 304
T+P +S + S I +TSN N+ S F S + + +S+ I + LN+
Sbjct: 3801 TSPSLSETIPQSSSISEASTSNPNILSSTVLSFDSTITNS----FTTASTSIMKTTSLNS 3856
Query: 303 A*S*PWRSSDGSLI*WYNLQKSSTVSNVVNSIRFSSHVL 187
S W +S + +YN SSTV ++ R +S+ +
Sbjct: 3857 N-SYKWYNSTTQSV-YYN-ATSSTVPWSNSTFRNTSNTM 3892
>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2685
Score = 25.8 bits (54), Expect = 7.2
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 125 FLIKHPLQNQWSLWFYDNDRNKTWEENLIELTTF 226
F I+ L+ + FY N +NK W N +L TF
Sbjct: 511 FEIESSLRLSSNKVFYHNPKNKKWLINTSDLITF 544
>SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein
Mam3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 25.8 bits (54), Expect = 7.2
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +3
Query: 132 SSTRYKTSGVSGFTTMTETKHG 197
++T Y TSG GFTT T+ G
Sbjct: 647 TTTEYTTSGSVGFTTTLATQSG 668
>SPAC1805.10 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 527
Score = 25.8 bits (54), Expect = 7.2
Identities = 13/38 (34%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Frame = +2
Query: 320 IRPMWEDDANKMGGRWLIS-LEKKQRFTDLDRFWLDVV 430
+ +WE N G +L +EKK F +DR+W V+
Sbjct: 161 VAALWEQ-FNIFHGSYLFDEIEKKSSFEIIDRWWTSVL 197
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,251,852
Number of Sequences: 5004
Number of extensions: 66356
Number of successful extensions: 192
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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