Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc10k16
(801 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g18040.1 68417.m02685 eukaryotic translation initiation facto... 158 5e-39
At1g29550.1 68414.m03614 eukaryotic translation initiation facto... 146 1e-35
At1g29590.1 68414.m03618 eukaryotic translation initiation facto... 145 3e-35
At5g35620.1 68418.m04251 eukaryotic translation initiation facto... 135 3e-32
At5g18110.1 68418.m02126 novel cap-binding protein (nCBP) identi... 123 1e-28
At5g35620.2 68418.m04252 eukaryotic translation initiation facto... 117 8e-27
>At4g18040.1 68417.m02685 eukaryotic translation initiation factor
4E 1 / eIF-4E1 / mRNA cap-binding protein 1 (EIF4E1)
identical to SP|O23252 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-4F 25 kDa subunit) (eIF-4F P26 subunit)
{Arabidopsis thaliana}
Length = 235
Score = 158 bits (383), Expect = 5e-39
Identities = 78/181 (43%), Positives = 110/181 (60%), Gaps = 4/181 (2%)
Frame = +2
Query: 137 HPLQNQWSLWFYDN----DRNKTWEENLIELTTFDTVEDFWRLYHHIKLPSELRQGHDYA 304
HPL++ W+ WF DN + +W +L + TF TVE+FW LY+++K PS+L G D+
Sbjct: 60 HPLEHSWTFWF-DNPAVKSKQTSWGSSLRPVFTFSTVEEFWSLYNNMKHPSKLAHGADFY 118
Query: 305 VFKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFENSDEICGAV 484
FK I P WED GG+W ++ K++ D+ WL +L LIGE F++ DEICGAV
Sbjct: 119 CFKHIIEPKWEDPICANGGKWTMTFPKEKS----DKSWLYTLLALIGEQFDHGDEICGAV 174
Query: 485 VNVRPKVDKIAIWTADAMKQHATIEIGKKLKEQLGIHGKIGFQVHRDTMVKHSSATKNLY 664
VN+R K ++I+IWT +A + A + IGK+ KE L + IGF +H D K KN Y
Sbjct: 175 VNIRGKQERISIWTKNASNEAAQVSIGKQWKEFLDYNNSIGFIIHEDAK-KLDRNAKNAY 233
Query: 665 T 667
T
Sbjct: 234 T 234
>At1g29550.1 68414.m03614 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 240
Score = 146 bits (354), Expect = 1e-35
Identities = 76/182 (41%), Positives = 103/182 (56%), Gaps = 4/182 (2%)
Frame = +2
Query: 137 HPLQNQWSLWFYDNDRNKT----WEENLIELTTFDTVEDFWRLYHHIKLPSELRQGHDYA 304
H QN W+ WF DN +K+ W +L L TF T+E+FW LY++I P++ G D
Sbjct: 65 HCFQNSWTFWF-DNPSSKSNQVIWGSSLRSLYTFGTIEEFWSLYNNIHPPTKWVSGADLY 123
Query: 305 VFKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFENSDEICGAV 484
FK I P WED GG+W + K L+ WL+ +L L+GE F+ DEICGAV
Sbjct: 124 CFKDKIEPKWEDPICANGGKWSMMFPK----ATLECNWLNTLLALVGEQFDQGDEICGAV 179
Query: 485 VNVRPKVDKIAIWTADAMKQHATIEIGKKLKEQLGIHGKIGFQVHRDTMVKHSSATKNLY 664
+N R + D+I++WT +A + A + IGK+ KE LG + IGF VH D A K Y
Sbjct: 180 LNFRARGDRISLWTKNAANEEAQLSIGKQWKELLGYNETIGFIVHEDAKTLDRDA-KRRY 238
Query: 665 TV 670
TV
Sbjct: 239 TV 240
>At1g29590.1 68414.m03618 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 285
Score = 145 bits (352), Expect = 3e-35
Identities = 75/182 (41%), Positives = 102/182 (56%), Gaps = 4/182 (2%)
Frame = +2
Query: 137 HPLQNQWSLWFYDNDRNKT----WEENLIELTTFDTVEDFWRLYHHIKLPSELRQGHDYA 304
H QN W+ WF DN +K+ W +L L TF T+E+FW LY++I P++ G D
Sbjct: 110 HCFQNSWTFWF-DNPSSKSNQVIWGSSLRSLYTFATIEEFWSLYNNIHPPTKWVSGSDLY 168
Query: 305 VFKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFENSDEICGAV 484
FK I P WED GG+W + + L+ WL+ +L L+GE F+ DEICGAV
Sbjct: 169 CFKDKIEPKWEDPICANGGKWTMFFPR----ATLESNWLNTLLALVGEQFDQGDEICGAV 224
Query: 485 VNVRPKVDKIAIWTADAMKQHATIEIGKKLKEQLGIHGKIGFQVHRDTMVKHSSATKNLY 664
+N R + D+I++WT A + A + IGK+ KE LG + IGF VH D A K Y
Sbjct: 225 LNFRTRGDRISLWTKKAANEEAQLSIGKQWKELLGYNDTIGFIVHEDAKTLDRDA-KRRY 283
Query: 665 TV 670
TV
Sbjct: 284 TV 285
>At5g35620.1 68418.m04251 eukaryotic translation initiation factor
4E 2 / eIF-4E2 / mRNA cap-binding protein 2 (EIF4E2)
identical to SP|O04663 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-(iso)4F 25 kDa subunit) (eIF-(ISO)4F P28 subunit)
(eIF4Eiso protein) {Arabidopsis thaliana}
Length = 198
Score = 135 bits (327), Expect = 3e-32
Identities = 70/170 (41%), Positives = 99/170 (58%), Gaps = 6/170 (3%)
Frame = +2
Query: 137 HPLQNQWSLWFYDNDRNK--TWEENLIELTTFDTVEDFWRLYHHIKLPSELRQGHDYAVF 310
H L+ +WS WF DN K W +L + TFDTVEDFW L+ I S+L + +F
Sbjct: 26 HKLERKWSFWF-DNQSKKGAAWGASLRKAYTFDTVEDFWGLHETIFQTSKLTANAEIHLF 84
Query: 311 KQGIRPMWEDDANKMGGR--WLISLEKKQRFTDLDRFWLDVVLLLIGENFENSDEICGAV 484
K G+ P WED GG+ W+++ +K+ LD+ WL+ ++ LIGE F+ +DEICG V
Sbjct: 85 KAGVEPKWEDPECANGGKWTWVVTANRKEA---LDKGWLETLMALIGEQFDEADEICGVV 141
Query: 485 VNVRP--KVDKIAIWTADAMKQHATIEIGKKLKEQLGIHGKIGFQVHRDT 628
+VRP K DK+++WT + + IGKK KE L + KI F H D+
Sbjct: 142 ASVRPQSKQDKLSLWTRTKSNEAVLMGIGKKWKEILDVTDKITFNNHDDS 191
>At5g18110.1 68418.m02126 novel cap-binding protein (nCBP) identical
to novel cap-binding protein nCBP [Arabidopsis thaliana]
GI:3108209; contains Pfam profile PF01652: Eukaryotic
initiation factor 4E
Length = 221
Score = 123 bits (297), Expect = 1e-28
Identities = 62/180 (34%), Positives = 101/180 (56%), Gaps = 4/180 (2%)
Frame = +2
Query: 137 HPLQNQWSLWFYDND---RNKTWEENLIELTTFDTVEDFWRLYHHIKLPSELRQGHDYAV 307
HPL+ ++S+W+ RN+++E+N+ ++ F TVE FW Y H+ S L D
Sbjct: 43 HPLRYKFSIWYTRRTPGVRNQSYEDNIKKMVEFSTVEGFWACYCHLARSSLLPSPTDLHF 102
Query: 308 FKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFENSDEICGAVV 487
FK GIRP+WED AN GG+W+I K RFW D++L L+G+ +++D ICGAV+
Sbjct: 103 FKDGIRPLWEDGANCNGGKWIIRFSK----VVSARFWEDLLLALVGDQLDDADNICGAVL 158
Query: 488 NVRPKVDKIAIWTADAMKQHATIEIGKKLKEQLGI-HGKIGFQVHRDTMVKHSSATKNLY 664
+VR D I++W +A A + + +K L + H + D ++ +S+ +N +
Sbjct: 159 SVRFNEDIISVWNRNASDHQAVMGLRDSIKRHLKLPHAYVMEYKPHDASLRDNSSYRNTW 218
>At5g35620.2 68418.m04252 eukaryotic translation initiation factor
4E 2 / eIF-4E2 / mRNA cap-binding protein 2 (EIF4E2)
identical to SP|O04663 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-(iso)4F 25 kDa subunit) (eIF-(ISO)4F P28 subunit)
(eIF4Eiso protein) {Arabidopsis thaliana}
Length = 167
Score = 117 bits (282), Expect = 8e-27
Identities = 58/136 (42%), Positives = 83/136 (61%), Gaps = 6/136 (4%)
Frame = +2
Query: 137 HPLQNQWSLWFYDNDRNK--TWEENLIELTTFDTVEDFWRLYHHIKLPSELRQGHDYAVF 310
H L+ +WS WF DN K W +L + TFDTVEDFW L+ I S+L + +F
Sbjct: 26 HKLERKWSFWF-DNQSKKGAAWGASLRKAYTFDTVEDFWGLHETIFQTSKLTANAEIHLF 84
Query: 311 KQGIRPMWEDDANKMGGR--WLISLEKKQRFTDLDRFWLDVVLLLIGENFENSDEICGAV 484
K G+ P WED GG+ W+++ +K+ LD+ WL+ ++ LIGE F+ +DEICG V
Sbjct: 85 KAGVEPKWEDPECANGGKWTWVVTANRKEA---LDKGWLETLMALIGEQFDEADEICGVV 141
Query: 485 VNVRP--KVDKIAIWT 526
+VRP K DK+++WT
Sbjct: 142 ASVRPQSKQDKLSLWT 157
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,821,153
Number of Sequences: 28952
Number of extensions: 341852
Number of successful extensions: 851
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 815
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 837
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1814318400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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