BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmnc10k08 (733 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g57070.1 68418.m07124 hydroxyproline-rich glycoprotein family... 29 4.2 At2g38910.1 68415.m04783 calcium-dependent protein kinase, putat... 28 5.6 At5g24580.2 68418.m02903 copper-binding family protein similar t... 28 7.3 At5g24580.1 68418.m02902 copper-binding family protein similar t... 28 7.3 At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein, puta... 27 9.7 At1g17270.1 68414.m02103 expressed protein 27 9.7 >At5g57070.1 68418.m07124 hydroxyproline-rich glycoprotein family protein Common family members: At5g26070, At5g19800, At1g72790 [Arabidopsis thaliana] Length = 575 Score = 28.7 bits (61), Expect = 4.2 Identities = 12/38 (31%), Positives = 21/38 (55%) Frame = +2 Query: 581 YSVNEHFSKLDVAEKEILAADLSPPQLSVKPSAPPAEP 694 Y ++ SK ++ E+E ++ VKPS+PP +P Sbjct: 211 YQQFQNLSKTEIEEEESEPKEIQIDTFVVKPSSPPQQP 248 >At2g38910.1 68415.m04783 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium-dependent protein kinase, isoform AK1 (CDPK) [Arabidopsis thaliana] SWISS-PROT:Q06850; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 583 Score = 28.3 bits (60), Expect = 5.6 Identities = 20/64 (31%), Positives = 29/64 (45%) Frame = +2 Query: 515 KSVDDAQRRHKQNCKFVNAIEDYSVNEHFSKLDVAEKEILAADLSPPQLSVKPSAPPAEP 694 KS D R K+N K VN D S S +D A + PP + + PP +P Sbjct: 34 KSSKDESSR-KKNDKSVNG--DDSNGHVSSTVDPAPSTLPTPSTPPPPVKMANEEPPPKP 90 Query: 695 LTQH 706 +T++ Sbjct: 91 ITEN 94 >At5g24580.2 68418.m02903 copper-binding family protein similar to copper homeostasis factor gi:3168840 from Arabidopsis thaliana; contains Pfam profile PF00403: Heavy-metal-associated domain Length = 318 Score = 27.9 bits (59), Expect = 7.3 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = +2 Query: 593 EHFSKLDVAEKEILAADLSPPQLSVKPSAPPAEP 694 E K DVAE++ +AA+ P+ +P PP P Sbjct: 22 EEEKKKDVAEEKKVAAEEEKPKEEEEPQPPPPPP 55 >At5g24580.1 68418.m02902 copper-binding family protein similar to copper homeostasis factor gi:3168840 from Arabidopsis thaliana; contains Pfam profile PF00403: Heavy-metal-associated domain Length = 319 Score = 27.9 bits (59), Expect = 7.3 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = +2 Query: 593 EHFSKLDVAEKEILAADLSPPQLSVKPSAPPAEP 694 E K DVAE++ +AA+ P+ +P PP P Sbjct: 23 EEEKKKDVAEEKKVAAEEEKPKEEEEPQPPPPPP 56 >At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein, putative / HSC70, putative / HSP70, putative strong similarity to heat shock cognate 70 kd protein 1 SP:P22953 [Arabidopsis thaliana (Mouse-ear cress)] Length = 617 Score = 27.5 bits (58), Expect = 9.7 Identities = 17/45 (37%), Positives = 26/45 (57%) Frame = +2 Query: 509 KYKSVDDAQRRHKQNCKFVNAIEDYSVNEHFSKLDVAEKEILAAD 643 KYKS D+ HK+ + N +E+Y+ N + D+ EK + AAD Sbjct: 530 KYKSEDE---EHKKKVEAKNGLENYAYNVGNTLRDMGEK-LPAAD 570 >At1g17270.1 68414.m02103 expressed protein Length = 564 Score = 27.5 bits (58), Expect = 9.7 Identities = 18/49 (36%), Positives = 27/49 (55%), Gaps = 4/49 (8%) Frame = +3 Query: 294 KLNDTHI-RIIAYHQPTR*CSMNR*EKNHLRVLKNLGVSLH---HNPWS 428 K N+ HI R I Y + C + +++H++ LK LGVS+ PWS Sbjct: 287 KKNNAHIDRFICYVSSPQPCYV---DEDHIKKLKGLGVSIGGKLEAPWS 332 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,279,629 Number of Sequences: 28952 Number of extensions: 303143 Number of successful extensions: 1004 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 960 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1004 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1604469728 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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