Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc10k04
         (699 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC354.01 |gtp1|SPBC649.06|GTP binding protein Gtp1|Schizosacch...   203   2e-53
SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual    173   2e-44
SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pom...    48   2e-06
SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1 |Schizosa...    38   0.001
SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ...    37   0.003
SPAC683.02c ||SPAC694.01c|zf-CCHC type zinc finger protein|Schiz...    27   2.6  
SPAC57A10.02 |cdr2||GIN4 family protein kinase Cdr2|Schizosaccha...    27   2.6  
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p...    26   4.5  
SPBC16G5.08 |trp4||phosphoribosylanthranilate transferase Trp4|S...    25   7.9  

>SPBC354.01 |gtp1|SPBC649.06|GTP binding protein
           Gtp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  203 bits (495), Expect = 2e-53
 Identities = 101/142 (71%), Positives = 112/142 (78%)
 Frame = +2

Query: 269 RTQKNKATEYHLGLLKAKLAKYRSQLLEPSKKGGDKGEGFDVLKSGDARVALIGFPSVGK 448
           RTQKNKATEYHLGLLK KLAK R+QLLEP+ K G KGEGFDVLKSGDARVA IGFPSVGK
Sbjct: 16  RTQKNKATEYHLGLLKGKLAKLRAQLLEPTSKSGPKGEGFDVLKSGDARVAFIGFPSVGK 75

Query: 449 XXXXXXXXXXXXEAASYEFTTLTCIPGVIEYRGANIQLLDLPGIIEGAAQGKGRGRQVIA 628
                         ASYEFTTLT IPGV+EY GA IQ+LDLPGIIEGA+QG+G GRQ ++
Sbjct: 76  STLLSAITKTKSATASYEFTTLTAIPGVLEYDGAEIQMLDLPGIIEGASQGRG-GRQAVS 134

Query: 629 VARTADLVLMMLDATKPYVHRQ 694
            ARTADL+LM+LDATK    R+
Sbjct: 135 AARTADLILMVLDATKAADQRE 156


>SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 366

 Score =  173 bits (422), Expect = 2e-44
 Identities = 82/144 (56%), Positives = 104/144 (72%)
 Frame = +2

Query: 266 ARTQKNKATEYHLGLLKAKLAKYRSQLLEPSKKGGDKGEGFDVLKSGDARVALIGFPSVG 445
           A+TQKNKAT  HLG+LKAKLAK + +L+ P+  GG  G GFDV ++G   V  IGFPSVG
Sbjct: 16  AKTQKNKATAKHLGMLKAKLAKLKRELITPTGGGGGGGLGFDVARTGIGTVGFIGFPSVG 75

Query: 446 KXXXXXXXXXXXXEAASYEFTTLTCIPGVIEYRGANIQLLDLPGIIEGAAQGKGRGRQVI 625
           K            EAA+YEFTTLT +PGV++Y GA IQ+LDLPGIIEGA  G+GRG+QVI
Sbjct: 76  KSTLMTQLTGTRSEAAAYEFTTLTTVPGVLQYNGAKIQILDLPGIIEGAKDGRGRGKQVI 135

Query: 626 AVARTADLVLMMLDATKPYVHRQL 697
            VART +L+ ++LD  KP  H+++
Sbjct: 136 TVARTCNLIFIVLDVLKPMSHKRI 159


>SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 419

 Score = 47.6 bits (108), Expect = 2e-06
 Identities = 26/90 (28%), Positives = 43/90 (47%), Gaps = 6/90 (6%)
 Frame = +2

Query: 416 VALIGFPSVGKXXXXXXXXXXXXEAASYEFTTLTCIPGVI------EYRGANIQLLDLPG 577
           + L+G P+ GK            +   YEFTT+    G I      ++     +L D+PG
Sbjct: 239 IGLVGLPNAGKSTLLNCLTASKSKVGEYEFTTIYPKIGTIKTTMPDDHSSFQYRLADIPG 298

Query: 578 IIEGAAQGKGRGRQVIAVARTADLVLMMLD 667
           II+GA+ GKG G   +     A ++ +++D
Sbjct: 299 IIKGASDGKGLGYDFLRHVERAKMLCLVID 328


>SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 642

 Score = 37.9 bits (84), Expect = 0.001
 Identities = 23/93 (24%), Positives = 42/93 (45%), Gaps = 3/93 (3%)
 Frame = +2

Query: 407 DARVALI-GFPSVGKXXXXXXXXXXXXEAASYEFTTLTCIPGVIEYRGANIQLLDLPGII 583
           + R  L+ G+P+VGK            +   Y FTT +   G  +Y+    Q++D PGI+
Sbjct: 167 NTRTLLVCGYPNVGKSSFMNKVTRAQVDVQPYAFTTKSLFVGHFDYKYLRWQVIDTPGIL 226

Query: 584 EGAAQGKG--RGRQVIAVARTADLVLMMLDATK 676
           +   +       + + A+A     VL  +D ++
Sbjct: 227 DHPLEQMNTIEMQSITAMAHLRSAVLYFMDLSE 259


>SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 409

 Score = 36.7 bits (81), Expect = 0.003
 Identities = 18/44 (40%), Positives = 27/44 (61%)
 Frame = +2

Query: 542 RGANIQLLDLPGIIEGAAQGKGRGRQVIAVARTADLVLMMLDAT 673
           R   IQLLD+ G+I GA  GKG G + +   R AD ++ ++D +
Sbjct: 75  RSIPIQLLDVAGLIPGAHAGKGLGNKFLDDLRHADALVHVVDVS 118


>SPAC683.02c ||SPAC694.01c|zf-CCHC type zinc finger
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 218

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 11/31 (35%), Positives = 22/31 (70%), Gaps = 1/31 (3%)
 Frame = +3

Query: 207 DKLAESWGF*RKYQKLRKKSR-EHRKIKRLN 296
           D +  S+G  ++Y + +KK R E+R+++R+N
Sbjct: 41  DNMKASFGSSKRYDERQKKKRSEYRRLRRIN 71


>SPAC57A10.02 |cdr2||GIN4 family protein kinase
           Cdr2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 775

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 12/24 (50%), Positives = 14/24 (58%)
 Frame = -3

Query: 229 PHDSASLSDNYLPRSQLIDNVFRL 158
           PHDS+ LS NY P S L    F +
Sbjct: 438 PHDSSMLSSNYRPPSALSPRNFNV 461


>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 676

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 8/19 (42%), Positives = 15/19 (78%)
 Frame = +1

Query: 64  NYILDRH*IKFKNLYSFLV 120
           N++L+ H ++F +LY F+V
Sbjct: 295 NFVLENHLVRFSSLYIFIV 313


>SPBC16G5.08 |trp4||phosphoribosylanthranilate transferase
           Trp4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 354

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 15/33 (45%), Positives = 19/33 (57%)
 Frame = -1

Query: 159 YLIKTEFTVSIISY*KTIQILKFYLMPIQNIIM 61
           +L K E    I+   +T+QILK Y  PI NI M
Sbjct: 53  HLTKAEEVPDILM--QTVQILKSYSTPIANIEM 83


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,458,025
Number of Sequences: 5004
Number of extensions: 44731
Number of successful extensions: 129
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)