Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc10k04
(699 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g17470.1 68414.m02143 developmentally regulated GTP-binding p... 202 2e-52
At1g72660.1 68414.m08403 developmentally regulated GTP-binding p... 201 4e-52
At4g39520.1 68417.m05588 GTP-binding protein, putative similar t... 199 2e-51
At5g18570.1 68418.m02195 GTP1/OBG family protein similar to SP|P... 48 6e-06
At1g07620.1 68414.m00817 GTP1/OBG family protein similar to SP|P... 46 2e-05
At1g50920.1 68414.m05725 GTP-binding protein-related similar to ... 39 0.003
At1g10300.1 68414.m01160 GTP-binding protein-related contains si... 34 0.10
At1g80770.1 68414.m09476 expressed protein 33 0.18
At2g01440.1 68415.m00067 ATP-dependent DNA helicase, putative 29 3.0
At5g43060.1 68418.m05256 cysteine proteinase, putative / thiol p... 28 5.2
At4g31630.1 68417.m04493 transcriptional factor B3 family protei... 28 6.8
At5g61600.1 68418.m07729 ethylene-responsive element-binding fam... 27 9.0
>At1g17470.1 68414.m02143 developmentally regulated GTP-binding
protein (DRG1) identical to developmentally regulated
GTP binding protein (DRG1) [Arabidopsis thaliana]
GI:2345150
Length = 399
Score = 202 bits (493), Expect = 2e-52
Identities = 101/144 (70%), Positives = 112/144 (77%)
Frame = +2
Query: 266 ARTQKNKATEYHLGLLKAKLAKYRSQLLEPSKKGGDKGEGFDVLKSGDARVALIGFPSVG 445
ARTQKNKATEYHLG LKAK+AK R+QLLEP K GEGF+V K G RVALIGFPSVG
Sbjct: 15 ARTQKNKATEYHLGQLKAKIAKLRTQLLEPPKGASGGGEGFEVTKYGHGRVALIGFPSVG 74
Query: 446 KXXXXXXXXXXXXEAASYEFTTLTCIPGVIEYRGANIQLLDLPGIIEGAAQGKGRGRQVI 625
K EAASYEFTTLTCIPGVI Y IQLLDLPGIIEGA++GKGRGRQVI
Sbjct: 75 KSTLLTMLTGTHSEAASYEFTTLTCIPGVIHYNDTKIQLLDLPGIIEGASEGKGRGRQVI 134
Query: 626 AVARTADLVLMMLDATKPYVHRQL 697
AVA+++DLVLM+LDA+K HRQ+
Sbjct: 135 AVAKSSDLVLMVLDASKSEGHRQI 158
>At1g72660.1 68414.m08403 developmentally regulated GTP-binding
protein, putative very strong similarity to
developmentally regulated GTP binding protein (DRG1)
[Arabidopsis thaliana] GI:2345150
Length = 399
Score = 201 bits (490), Expect = 4e-52
Identities = 100/144 (69%), Positives = 112/144 (77%)
Frame = +2
Query: 266 ARTQKNKATEYHLGLLKAKLAKYRSQLLEPSKKGGDKGEGFDVLKSGDARVALIGFPSVG 445
ARTQKNKATEYHLG LKAK+AK R+QLLEP K G+GF+V K G RVALIGFPSVG
Sbjct: 15 ARTQKNKATEYHLGQLKAKIAKLRTQLLEPPKGSSGGGDGFEVTKYGHGRVALIGFPSVG 74
Query: 446 KXXXXXXXXXXXXEAASYEFTTLTCIPGVIEYRGANIQLLDLPGIIEGAAQGKGRGRQVI 625
K EAASYEFTTLTCIPGVI Y IQLLDLPGIIEGA++GKGRGRQVI
Sbjct: 75 KSTLLTMLTGTHSEAASYEFTTLTCIPGVIHYNDTKIQLLDLPGIIEGASEGKGRGRQVI 134
Query: 626 AVARTADLVLMMLDATKPYVHRQL 697
AVA+++DLVLM+LDA+K HRQ+
Sbjct: 135 AVAKSSDLVLMVLDASKSEGHRQI 158
>At4g39520.1 68417.m05588 GTP-binding protein, putative similar to
SP|Q9Y295 Developmentally regulated GTP-binding protein
1 (DRG 1) {Homo sapiens}; contains Pfam profiles
PF02824: TGS domain, PF01018: GTP1/OBG family
Length = 369
Score = 199 bits (485), Expect = 2e-51
Identities = 99/146 (67%), Positives = 110/146 (75%), Gaps = 2/146 (1%)
Frame = +2
Query: 266 ARTQKNKATEYHLGLLKAKLAKYRSQLLEPSKKGGD--KGEGFDVLKSGDARVALIGFPS 439
A+TQKNKAT +HLGLLKAKLAK R LL P KGG GEGFDV KSGD+RV L+GFPS
Sbjct: 16 AKTQKNKATSHHLGLLKAKLAKLRRDLLAPPTKGGGGGAGEGFDVTKSGDSRVGLVGFPS 75
Query: 440 VGKXXXXXXXXXXXXEAASYEFTTLTCIPGVIEYRGANIQLLDLPGIIEGAAQGKGRGRQ 619
VGK E ASYEFTTLTCIPGVI YRGA IQLLDLPGIIEGA GKGRGRQ
Sbjct: 76 VGKSTLLNKLTGTFSEVASYEFTTLTCIPGVITYRGAKIQLLDLPGIIEGAKDGKGRGRQ 135
Query: 620 VIAVARTADLVLMMLDATKPYVHRQL 697
VI+ ART + +L++LDA KP H++L
Sbjct: 136 VISTARTCNCILIVLDAIKPITHKRL 161
>At5g18570.1 68418.m02195 GTP1/OBG family protein similar to
SP|P20964 Spo0B-associated GTP-binding protein {Bacillus
subtilis}; contains Pfam profile PF01018: GTP1/OBG
family
Length = 681
Score = 48.0 bits (109), Expect = 6e-06
Identities = 26/91 (28%), Positives = 45/91 (49%), Gaps = 1/91 (1%)
Frame = +2
Query: 410 ARVALIGFPSVGKXXXXXXXXXXXXEAASYEFTTLTCIPGVIEYR-GANIQLLDLPGIIE 586
A V ++G P+ GK A+Y FTTL GV+ + + + + DLPG++E
Sbjct: 379 ADVGIVGAPNAGKSTLLSVISAAQPTIANYPFTTLLPNLGVVSFDYDSTMVVADLPGLLE 438
Query: 587 GAAQGKGRGRQVIAVARTADLVLMMLDATKP 679
GA +G G G + + ++ ++D + P
Sbjct: 439 GAHRGFGLGHEFLRHTERCSALVHVVDGSAP 469
>At1g07620.1 68414.m00817 GTP1/OBG family protein similar to SP|P20964
Spo0B-associated GTP-binding protein {Bacillus subtilis};
contains Pfam profile PF01018: GTP1/OBG family
Length = 1016
Score = 46.4 bits (105), Expect = 2e-05
Identities = 26/85 (30%), Positives = 40/85 (47%), Gaps = 1/85 (1%)
Frame = +2
Query: 410 ARVALIGFPSVGKXXXXXXXXXXXXEAASYEFTTLTCIPGVIEYRGANIQLLDLPGIIEG 589
A V L+G P+ GK Y FTTL G + Y ++ + D+PG+I+G
Sbjct: 827 ADVGLVGMPNAGKSTLLGALSRAKPRVGHYAFTTLRPNLGNVNYDDFSMTVADIPGLIKG 886
Query: 590 AAQGKGRGRQVIA-VARTADLVLMM 661
A Q +G G + + RT L ++
Sbjct: 887 AHQNRGLGHNFLRHIERTKVLAYVV 911
>At1g50920.1 68414.m05725 GTP-binding protein-related similar to
GTP-binding protein SP:Q99ME9 from [Mus musculus]
Length = 671
Score = 39.1 bits (87), Expect = 0.003
Identities = 23/86 (26%), Positives = 38/86 (44%), Gaps = 2/86 (2%)
Frame = +2
Query: 416 VALIGFPSVGKXXXXXXXXXXXXEAASYEFTTLTCIPGVIEYRGANIQLLDLPGIIEGAA 595
V + G+P+VGK + Y FTT + G +Y+ Q++D PGI++
Sbjct: 171 VLICGYPNVGKSSFMNKVTRADVDVQPYAFTTKSLFVGHTDYKYLRYQVIDTPGILDRPF 230
Query: 596 QGKG--RGRQVIAVARTADLVLMMLD 667
+ + + A+A VL LD
Sbjct: 231 EDRNIIEMCSITALAHLRAAVLFFLD 256
>At1g10300.1 68414.m01160 GTP-binding protein-related contains
similarity to nucleolar GTP-binding protein 1 SP: Q9BZE4
from [Homo sapiens];
Length = 687
Score = 33.9 bits (74), Expect = 0.10
Identities = 24/90 (26%), Positives = 38/90 (42%), Gaps = 3/90 (3%)
Frame = +2
Query: 407 DARVALI-GFPSVGKXXXXXXXXXXXXEAASYEFTTLTCIPGVIEYRGANIQLLDLPGII 583
+ R LI G P+VGK Y FTT + G +Y+ Q++D PG++
Sbjct: 196 NTRTLLICGCPNVGKSSFMNKVTRADVAVQPYAFTTKSLFLGHTDYKCLRYQVIDTPGLL 255
Query: 584 EGAAQGKG--RGRQVIAVARTADLVLMMLD 667
+ + + + A+A VL LD
Sbjct: 256 DREIEDRNIIELCSITALAHIRAAVLFFLD 285
>At1g80770.1 68414.m09476 expressed protein
Length = 451
Score = 33.1 bits (72), Expect = 0.18
Identities = 17/56 (30%), Positives = 25/56 (44%)
Frame = +2
Query: 416 VALIGFPSVGKXXXXXXXXXXXXEAASYEFTTLTCIPGVIEYRGANIQLLDLPGII 583
+ L+G P+VGK E +Y FTT + G I Q+ D PG++
Sbjct: 256 LCLVGAPNVGKSSLVRILSTGKPEICNYPFTTRGILMGHIVLNYQRFQVTDTPGLL 311
>At2g01440.1 68415.m00067 ATP-dependent DNA helicase, putative
Length = 953
Score = 29.1 bits (62), Expect = 3.0
Identities = 14/47 (29%), Positives = 22/47 (46%)
Frame = +2
Query: 284 KATEYHLGLLKAKLAKYRSQLLEPSKKGGDKGEGFDVLKSGDARVAL 424
+A L ++ K KY LL K DK E + +SG+ ++ L
Sbjct: 781 RAASAELEIVTKKFPKYNCGLLHGRMKSDDKEEALNKFRSGETQILL 827
>At5g43060.1 68418.m05256 cysteine proteinase, putative / thiol
protease, putative similar to cysteine proteinase RD21A
precursor (thiol protease) GI:435619, SP:P43297 from
[Arabidopsis thaliana]
Length = 463
Score = 28.3 bits (60), Expect = 5.2
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = -1
Query: 429 PIKATRASPDFSTSKPSPLSPPFFEGSSNCDLYFA 325
PIK + P+ S PSP+ PP + CD YF+
Sbjct: 352 PIKKGQNPPNPGPSPPSPIKPP-----TTCDKYFS 381
>At4g31630.1 68417.m04493 transcriptional factor B3 family protein
similar to reproductive meristem gene 1 from [Brassica
oleracea var. botrytis] GI:3170424, [Arabidopsis
thaliana] GI:13604227; contains Pfam profile PF02362: B3
DNA binding domain
Length = 512
Score = 27.9 bits (59), Expect = 6.8
Identities = 13/34 (38%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Frame = -1
Query: 468 RVDKRVDLPTEGKPIKATRASP-DFSTSKPSPLS 370
RV+KR + EG P ++TRA P + +P +S
Sbjct: 435 RVEKRAQVTEEGGPSRSTRADPGNLQQKQPCSIS 468
>At5g61600.1 68418.m07729 ethylene-responsive element-binding family
protein contains similarity to ethylene responsive
element binding factor 5 (AtERF5) (Swiss-Prot:O80341)
[Arabidopsis thaliana]; contains Pfam PF00847: AP2
domain
Length = 241
Score = 27.5 bits (58), Expect = 9.0
Identities = 9/33 (27%), Positives = 21/33 (63%)
Frame = +2
Query: 269 RTQKNKATEYHLGLLKAKLAKYRSQLLEPSKKG 367
+T+K + ++ G+ + KY +++ +P+KKG
Sbjct: 78 KTEKEEEERHYRGVRRRPWGKYAAEIRDPNKKG 110
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,458,122
Number of Sequences: 28952
Number of extensions: 221343
Number of successful extensions: 559
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 539
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 556
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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