SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc10k02
         (606 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC26A3.04 |rpl2002|rpl20, rpl20-2|60S ribosomal protein L20|Sc...   176   2e-45
SPAC3A12.10 |rpl2001|rpl20-1, rpl20, yl17b, rpl18a-2|60S ribosom...   176   2e-45
SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting pro...    29   0.53 
SPBC1718.07c |zfs1|moc4|transcription factor Zfs1 |Schizosacchar...    28   1.2  
SPAC22E12.11c |set3||histone lysine methyltransferase Set3|Schiz...    26   3.7  
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb...    26   4.9  
SPBC8D2.05c |sfi1||spindle pole body protein Sfi1|Schizosaccharo...    25   6.5  
SPAC4H3.11c |ppc89|mug127|spindle pole body protein Ppc89|Schizo...    25   6.5  
SPAC10F6.13c |||aspartate aminotransferase |Schizosaccharomyces ...    25   6.5  
SPAC57A7.08 |pzh1||serine/threonine protein phosphatase Pzh1|Sch...    25   6.5  
SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces...    25   8.6  
SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|c...    25   8.6  

>SPAC26A3.04 |rpl2002|rpl20, rpl20-2|60S ribosomal protein
           L20|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 176

 Score =  176 bits (428), Expect = 2e-45
 Identities = 89/174 (51%), Positives = 114/174 (65%), Gaps = 4/174 (2%)
 Frame = +1

Query: 49  LREYEVIGRKLPSENEPKPPLYKMRIFSPDPIVAKSRFWYFLRQLKKFKKTTGEIVXXXX 228
           L+EY+V+GRK+P+E+EP P L++MR+F+P+  VAKSR+WYFL+ + K KK TGEIV    
Sbjct: 3   LKEYQVVGRKVPTEHEPVPKLFRMRLFAPNESVAKSRYWYFLKMINKVKKATGEIVAINE 62

Query: 229 XXXXXXXXXXNFGIWLRYESRSGVHNMYREYRDLSVGGAVTQCYRDMGARHRARAHSIQI 408
                      FGIW+RY+SRSG HNMY+E+RD +  GAV   Y DM ARHRAR  SI+I
Sbjct: 63  ISEPKPLKAKVFGIWIRYDSRSGTHNMYKEFRDTTRVGAVEAMYADMAARHRARFRSIRI 122

Query: 409 IK-VEVIKAAACRRPQVKQFHNSTIRFPLPKR---VHHYKRLNTFAYKRPSTYF 558
           +K VEV K    RR  VKQ  N  ++FPLP R   V        FA  RPST++
Sbjct: 123 LKVVEVEKKEDVRRNYVKQLLNPHLKFPLPHRRTGVVGLAGKKVFAPHRPSTFY 176


>SPAC3A12.10 |rpl2001|rpl20-1, rpl20, yl17b, rpl18a-2|60S ribosomal
           protein L20a|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 176

 Score =  176 bits (428), Expect = 2e-45
 Identities = 89/174 (51%), Positives = 114/174 (65%), Gaps = 4/174 (2%)
 Frame = +1

Query: 49  LREYEVIGRKLPSENEPKPPLYKMRIFSPDPIVAKSRFWYFLRQLKKFKKTTGEIVXXXX 228
           L+EY+V+GRK+P+E+EP P L++MR+F+P+  VAKSR+WYFL+ + K KK TGEIV    
Sbjct: 3   LKEYQVVGRKVPTEHEPVPKLFRMRLFAPNESVAKSRYWYFLKMINKVKKATGEIVAINE 62

Query: 229 XXXXXXXXXXNFGIWLRYESRSGVHNMYREYRDLSVGGAVTQCYRDMGARHRARAHSIQI 408
                      FGIW+RY+SRSG HNMY+E+RD +  GAV   Y DM ARHRAR  SI+I
Sbjct: 63  ISEPKPLKAKVFGIWIRYDSRSGTHNMYKEFRDTTRVGAVEAMYADMAARHRARFRSIRI 122

Query: 409 IK-VEVIKAAACRRPQVKQFHNSTIRFPLPKR---VHHYKRLNTFAYKRPSTYF 558
           +K VEV K    RR  VKQ  N  ++FPLP R   V        FA  RPST++
Sbjct: 123 LKVVEVEKKEDVRRNYVKQLLNPHLKFPLPHRRTGVVGLAGKKVFAPHRPSTFY 176


>SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting
           protein 3 homolog Bud6|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1385

 Score = 29.1 bits (62), Expect = 0.53
 Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 3/57 (5%)
 Frame = +2

Query: 128 SLRIQLSPNLVSGIS*DNLRSSRRPLVKLSLLRKFQRK---APSRLRTLVSGYAMSL 289
           S ++   P++V   S D+ + SRRP +  S  RKF  K    PS  ++L  G   SL
Sbjct: 295 SPKVSKRPSIVRQDSHDSNKLSRRPTINTSFDRKFSPKLTRTPSLTKSLDPGTPTSL 351


>SPBC1718.07c |zfs1|moc4|transcription factor Zfs1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 404

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 17/48 (35%), Positives = 22/48 (45%)
 Frame = -3

Query: 370 PYLYSTE*QLHLHSGHGIHGTCCVHPNETHSVARYQSS*S*WGFSLEF 227
           P L S+    +LHSG   HG    HP+   S+    S  S    SL+F
Sbjct: 125 PSLLSSNATSYLHSGSSPHGNTSNHPSPISSLESLPSRSSTGSGSLDF 172


>SPAC22E12.11c |set3||histone lysine methyltransferase
           Set3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 859

 Score = 26.2 bits (55), Expect = 3.7
 Identities = 14/43 (32%), Positives = 21/43 (48%)
 Frame = +3

Query: 348 HSVL*RYGS*TQSPSSLNTDHQSGSNQGCCVSPSTGQTVPQQH 476
           HS   R G+ T +  +L +   + SN G   +  TG T+P  H
Sbjct: 99  HSASPRQGNNTGANGALFSQSTNSSNSGSYRNSVTGATLPNAH 141


>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1428

 Score = 25.8 bits (54), Expect = 4.9
 Identities = 11/39 (28%), Positives = 19/39 (48%)
 Frame = +1

Query: 52  REYEVIGRKLPSENEPKPPLYKMRIFSPDPIVAKSRFWY 168
           +E  V+   +  E     P   +R +SP+ ++  S FWY
Sbjct: 470 QEILVLKSSIEKEKVEIVPFLDIRKYSPNEVLCMSPFWY 508


>SPBC8D2.05c |sfi1||spindle pole body protein
           Sfi1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 840

 Score = 25.4 bits (53), Expect = 6.5
 Identities = 8/20 (40%), Positives = 13/20 (65%)
 Frame = +1

Query: 136 DPIVAKSRFWYFLRQLKKFK 195
           D ++   RFWY+L + K F+
Sbjct: 476 DRLIVTFRFWYYLMKAKSFQ 495


>SPAC4H3.11c |ppc89|mug127|spindle pole body protein
           Ppc89|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 783

 Score = 25.4 bits (53), Expect = 6.5
 Identities = 10/32 (31%), Positives = 19/32 (59%)
 Frame = -1

Query: 159 TRFGDNWIRRENSHFIQGRLGLVLRRELAADN 64
           ++F DN++  ++      R+G+ L REL  D+
Sbjct: 3   SQFNDNFVATDDDVGTMARVGMALDRELNGDS 34


>SPAC10F6.13c |||aspartate aminotransferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 409

 Score = 25.4 bits (53), Expect = 6.5
 Identities = 9/23 (39%), Positives = 11/23 (47%)
 Frame = -1

Query: 489 WESDGAVVELFDLWTATRSSLDY 421
           WE  G  V+ +  W A   S DY
Sbjct: 145 WEKLGVTVDTYPYWDAKNRSFDY 167


>SPAC57A7.08 |pzh1||serine/threonine protein phosphatase
           Pzh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 515

 Score = 25.4 bits (53), Expect = 6.5
 Identities = 16/51 (31%), Positives = 24/51 (47%)
 Frame = +3

Query: 369 GS*TQSPSSLNTDHQSGSNQGCCVSPSTGQTVPQQHHQIPIAQTCAPLQET 521
           G+ +QSP+S +      SNQ   +SPST  +    HH    +   +P   T
Sbjct: 93  GNSSQSPTSPHP-----SNQPAMLSPSTAASQHHHHHSSSSSYAVSPTSPT 138


>SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1441

 Score = 25.0 bits (52), Expect = 8.6
 Identities = 12/30 (40%), Positives = 16/30 (53%)
 Frame = -1

Query: 207  TSGLLELLKLSQEIPETRFGDNWIRRENSH 118
            TSG L LL    E PE+  G+  + R + H
Sbjct: 1274 TSGNLRLLAYDPENPESHSGERLVTRGDFH 1303


>SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 559

 Score = 25.0 bits (52), Expect = 8.6
 Identities = 7/24 (29%), Positives = 12/24 (50%)
 Frame = +1

Query: 103 PPLYKMRIFSPDPIVAKSRFWYFL 174
           PP     +  P  +  K +FWY++
Sbjct: 534 PPKSNAELVDPSTLSGKDKFWYYI 557


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,570,365
Number of Sequences: 5004
Number of extensions: 51982
Number of successful extensions: 131
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 266270664
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -