BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmnc10k01 (725 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g37060.1 68417.m05248 patatin, putative similar to patatin-li... 33 0.19 At5g35695.1 68418.m04268 hypothetical protein 31 0.78 At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain... 29 4.1 At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain... 29 4.1 At5g64070.1 68418.m08046 phosphatidylinositol 4-kinase (PI4K) ne... 27 9.6 At2g26340.1 68415.m03160 expressed protein 27 9.6 >At4g37060.1 68417.m05248 patatin, putative similar to patatin-like latex allergen [Hevea brasiliensis][PMID:10589016]; contains patatin domain PF01734 Length = 414 Score = 33.1 bits (72), Expect = 0.19 Identities = 28/84 (33%), Positives = 38/84 (45%), Gaps = 4/84 (4%) Frame = +3 Query: 243 TFEHADQIQHHAPDSVAK--QGDPLYLH-PHTVLITKSGVIQLIMKSKLPYAIELQEWLL 413 TFE + I H+ V K Q + YL L + + L KS L I+L E +L Sbjct: 295 TFESSRDIVHYHSSVVFKALQSEDKYLRIDDDTLEGDASTLDLSTKSNLENLIKLGEKML 354 Query: 414 EEVIPQV-LCTGKYAPAVEMDTND 482 + Q+ + TG Y PA E ND Sbjct: 355 TNRVMQMNIDTGTYEPAAENINND 378 >At5g35695.1 68418.m04268 hypothetical protein Length = 211 Score = 31.1 bits (67), Expect = 0.78 Identities = 21/61 (34%), Positives = 27/61 (44%) Frame = -1 Query: 686 HVLRNVRHAVGQLRRFAARVFAILPSRFSVASSHNHFVGKQNERSVGFRQICVGHRQFLR 507 H L N+RH LR R+F I SRF++ S F K + G C FLR Sbjct: 87 HELFNLRHV--SLRNVIERIFGIFKSRFAIFKSAPPFSYK---KQAGLVLTCAALHNFLR 141 Query: 506 Q 504 + Sbjct: 142 K 142 >At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing protein / RhoGAP domain-containing protein weak similarity to rho-GTPase activating protein [Homo sapiens] GI:14245732; contains Pfam profiles PF00169: PH domain, PF00620: RhoGAP domain Length = 870 Score = 28.7 bits (61), Expect = 4.1 Identities = 17/47 (36%), Positives = 22/47 (46%) Frame = +3 Query: 75 QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 215 Q G F DT +Y+ RF+ +D S+L YVN ER R Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735 >At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing protein / RhoGAP domain-containing protein weak similarity to rho-GTPase activating protein [Homo sapiens] GI:14245732; contains Pfam profiles PF00169: PH domain, PF00620: RhoGAP domain Length = 822 Score = 28.7 bits (61), Expect = 4.1 Identities = 17/47 (36%), Positives = 22/47 (46%) Frame = +3 Query: 75 QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 215 Q G F DT +Y+ RF+ +D S+L YVN ER R Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735 >At5g64070.1 68418.m08046 phosphatidylinositol 4-kinase (PI4K) nearly identical to gi:4467359 Length = 1121 Score = 27.5 bits (58), Expect = 9.6 Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%) Frame = +3 Query: 249 EH-ADQIQHHAPDSVAKQGDPLYLHPHTVLITKS 347 EH A Q+ H D + G PL+L P+ VL+T S Sbjct: 872 EHLAVQLISHFYDIFQEAGLPLWLRPYEVLVTSS 905 >At2g26340.1 68415.m03160 expressed protein Length = 230 Score = 27.5 bits (58), Expect = 9.6 Identities = 19/54 (35%), Positives = 27/54 (50%) Frame = +3 Query: 345 SGVIQLIMKSKLPYAIELQEWLLEEVIPQVLCTGKYAPAVEMDTNDVIAKIDDL 506 S ++MKS+L A+ + LL+ V QVL GK V ++ A DDL Sbjct: 165 SNATVVLMKSQLGTALTALDSLLQTVPSQVLDKGKAMVEVYRSASEEDAGSDDL 218 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,825,474 Number of Sequences: 28952 Number of extensions: 375870 Number of successful extensions: 997 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 970 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 997 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1584903024 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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