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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc10j18
         (669 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g31340.1 68414.m03835 ubiquitin family protein contains INTER...    43   2e-04
At2g35635.1 68415.m04370 ubiquitin family protein contains INTER...    40   0.001
At5g42220.1 68418.m05139 ubiquitin family protein contains INTER...    38   0.005
At1g53930.1 68414.m06139 ubiquitin family protein contains Pfam ...    31   0.69 
At5g20000.1 68418.m02380 26S proteasome AAA-ATPase subunit, puta...    30   1.6  
At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to polyu...    30   1.6  
At5g19990.1 68418.m02379 26S proteasome AAA-ATPase subunit (RPT6a)     29   2.8  
At5g54180.1 68418.m06746 mitochondrial transcription termination...    28   6.5  
At1g53950.1 68414.m06145 ubiquitin family protein contains INTER...    27   8.5  

>At1g31340.1 68414.m03835 ubiquitin family protein contains
           INTERPRO:IPR000626 ubiquitin domain
          Length = 156

 Score = 42.7 bits (96), Expect = 2e-04
 Identities = 28/70 (40%), Positives = 36/70 (51%)
 Frame = +2

Query: 290 ITVKQLQGGECLIDIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQMYPNI 469
           I VK L G E  IDI P+  I  +K  V  K  IP  QQ+         DD T + Y NI
Sbjct: 79  IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDY-NI 137

Query: 470 KEGTKLNLVV 499
           + G+ L+LV+
Sbjct: 138 EGGSVLHLVL 147


>At2g35635.1 68415.m04370 ubiquitin family protein contains
           INTERPRO:IPR000626 ubiquitin domain
          Length = 154

 Score = 39.9 bits (89), Expect = 0.001
 Identities = 27/70 (38%), Positives = 35/70 (50%)
 Frame = +2

Query: 290 ITVKQLQGGECLIDIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQMYPNI 469
           I VK L G E  IDI P+  I  +K  V  K  IP  QQ+         DD T + Y  I
Sbjct: 79  IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYA-I 137

Query: 470 KEGTKLNLVV 499
           + G+ L+LV+
Sbjct: 138 EGGSVLHLVL 147


>At5g42220.1 68418.m05139 ubiquitin family protein contains
           INTERPRO:IPR000626 ubiquitin domain
          Length = 879

 Score = 38.3 bits (85), Expect = 0.005
 Identities = 24/101 (23%), Positives = 44/101 (43%)
 Frame = +2

Query: 278 SKMKITVKQLQGGECLIDIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQM 457
           S +++ +K L        +  +  +   K  +A +  +PV QQ+         DDH +  
Sbjct: 22  STLELNIKTLDSRTYTFQVNKNETVLLFKEKIASETGVPVGQQRLIFRGRVLKDDHPLSE 81

Query: 458 YPNIKEGTKLNLVVKKPESLYDASFKHYKRQGMSDKDAANT 580
           Y +++ G  L+L+V++P     A       QG +  D  NT
Sbjct: 82  Y-HLENGHTLHLIVRQPAE--SAPSSGTPSQGATANDGNNT 119


>At1g53930.1 68414.m06139 ubiquitin family protein contains Pfam
           profile: PF00240 ubiquitin family
          Length = 158

 Score = 31.1 bits (67), Expect = 0.69
 Identities = 20/71 (28%), Positives = 34/71 (47%)
 Frame = +2

Query: 284 MKITVKQLQGGECLIDIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQMYP 463
           M I +K L G   ++++     I  +K  +  K  IPV+QQ+         D  T+  Y 
Sbjct: 86  MFIFIKTLTGRRIVLEVENCDTIENIKAKIQDKQGIPVDQQRLIFKGKQLEDGLTLADY- 144

Query: 464 NIKEGTKLNLV 496
           NI+  + L+L+
Sbjct: 145 NIQNDSILHLI 155


>At5g20000.1 68418.m02380 26S proteasome AAA-ATPase subunit,
           putative almost identical to 26S proteasome AAA-ATPase
           subunit RPT6a GI:6652888 from [Arabidopsis thaliana];
           almost identical to a member of conserved Sug1 CAD
           family AtSUG1 GI:13537115 from [Arabidopsis thaliana]
          Length = 419

 Score = 29.9 bits (64), Expect = 1.6
 Identities = 17/47 (36%), Positives = 30/47 (63%)
 Frame = +2

Query: 221 VLKTPGSFVTQYFKTVKIISKMKITVKQLQGGECLIDIYPSMLISEL 361
           +L+ PGS+V +    VK++ K K+ VK    G+ ++DI  S+ I++L
Sbjct: 77  LLQEPGSYVGE---VVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKL 120


>At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to
           polyubiquitin (ubq8) GI:870793, GB:L05917 [Arabidopsis
           thaliana] (Genetics 139 (2), 921-939 (1995))
          Length = 631

 Score = 29.9 bits (64), Expect = 1.6
 Identities = 20/73 (27%), Positives = 36/73 (49%)
 Frame = +2

Query: 284 MKITVKQLQGGECLIDIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQMYP 463
           ++I  K L      +D+  S  I  +K  +  K  IP++QQ+         D  T+  Y 
Sbjct: 3   IQIYAKTLTEKTITLDVETSDSIHNVKAKIQNKEGIPLDQQRLIFAGKQLEDGLTLADY- 61

Query: 464 NIKEGTKLNLVVK 502
           NI++ + L+LV++
Sbjct: 62  NIQKESTLHLVLR 74


>At5g19990.1 68418.m02379 26S proteasome AAA-ATPase subunit (RPT6a)
          Length = 419

 Score = 29.1 bits (62), Expect = 2.8
 Identities = 16/47 (34%), Positives = 30/47 (63%)
 Frame = +2

Query: 221 VLKTPGSFVTQYFKTVKIISKMKITVKQLQGGECLIDIYPSMLISEL 361
           +L+ PGS+V +    VK++ K K+ VK    G+ ++DI  S+ I+++
Sbjct: 77  LLQEPGSYVGE---VVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKI 120


>At5g54180.1 68418.m06746 mitochondrial transcription termination
           factor-related / mTERF-related contains Pfam profile
           PF02536: mTERF
          Length = 500

 Score = 27.9 bits (59), Expect = 6.5
 Identities = 12/44 (27%), Positives = 23/44 (52%)
 Frame = +2

Query: 521 DASFKHYKRQGMSDKDAANTANKLLRIVQEKFDKMSWDEVDRLC 652
           D+  KH   + +  +    + NKLL +  E+F K + D ++ +C
Sbjct: 457 DSRIKHRYEEKLKSRGENMSLNKLLTVSAERFSKAA-DNIEMIC 499


>At1g53950.1 68414.m06145 ubiquitin family protein contains
           INTERPRO:IPR000626 ubiquitin domain
          Length = 216

 Score = 27.5 bits (58), Expect = 8.5
 Identities = 17/42 (40%), Positives = 21/42 (50%)
 Frame = +2

Query: 284 MKITVKQLQGGECLIDIYPSMLISELKRHVARKLHIPVEQQK 409
           M I +K L G     ++  S  I ELK     K  IPVEQQ+
Sbjct: 50  MMIFIKTLTGRTNTYEVKGSDTIRELKAKHEEKEGIPVEQQR 91


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,597,087
Number of Sequences: 28952
Number of extensions: 233234
Number of successful extensions: 540
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 531
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 540
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1412971776
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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