BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmnc10j18 (669 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g31340.1 68414.m03835 ubiquitin family protein contains INTER... 43 2e-04 At2g35635.1 68415.m04370 ubiquitin family protein contains INTER... 40 0.001 At5g42220.1 68418.m05139 ubiquitin family protein contains INTER... 38 0.005 At1g53930.1 68414.m06139 ubiquitin family protein contains Pfam ... 31 0.69 At5g20000.1 68418.m02380 26S proteasome AAA-ATPase subunit, puta... 30 1.6 At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to polyu... 30 1.6 At5g19990.1 68418.m02379 26S proteasome AAA-ATPase subunit (RPT6a) 29 2.8 At5g54180.1 68418.m06746 mitochondrial transcription termination... 28 6.5 At1g53950.1 68414.m06145 ubiquitin family protein contains INTER... 27 8.5 >At1g31340.1 68414.m03835 ubiquitin family protein contains INTERPRO:IPR000626 ubiquitin domain Length = 156 Score = 42.7 bits (96), Expect = 2e-04 Identities = 28/70 (40%), Positives = 36/70 (51%) Frame = +2 Query: 290 ITVKQLQGGECLIDIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQMYPNI 469 I VK L G E IDI P+ I +K V K IP QQ+ DD T + Y NI Sbjct: 79 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDY-NI 137 Query: 470 KEGTKLNLVV 499 + G+ L+LV+ Sbjct: 138 EGGSVLHLVL 147 >At2g35635.1 68415.m04370 ubiquitin family protein contains INTERPRO:IPR000626 ubiquitin domain Length = 154 Score = 39.9 bits (89), Expect = 0.001 Identities = 27/70 (38%), Positives = 35/70 (50%) Frame = +2 Query: 290 ITVKQLQGGECLIDIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQMYPNI 469 I VK L G E IDI P+ I +K V K IP QQ+ DD T + Y I Sbjct: 79 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYA-I 137 Query: 470 KEGTKLNLVV 499 + G+ L+LV+ Sbjct: 138 EGGSVLHLVL 147 >At5g42220.1 68418.m05139 ubiquitin family protein contains INTERPRO:IPR000626 ubiquitin domain Length = 879 Score = 38.3 bits (85), Expect = 0.005 Identities = 24/101 (23%), Positives = 44/101 (43%) Frame = +2 Query: 278 SKMKITVKQLQGGECLIDIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQM 457 S +++ +K L + + + K +A + +PV QQ+ DDH + Sbjct: 22 STLELNIKTLDSRTYTFQVNKNETVLLFKEKIASETGVPVGQQRLIFRGRVLKDDHPLSE 81 Query: 458 YPNIKEGTKLNLVVKKPESLYDASFKHYKRQGMSDKDAANT 580 Y +++ G L+L+V++P A QG + D NT Sbjct: 82 Y-HLENGHTLHLIVRQPAE--SAPSSGTPSQGATANDGNNT 119 >At1g53930.1 68414.m06139 ubiquitin family protein contains Pfam profile: PF00240 ubiquitin family Length = 158 Score = 31.1 bits (67), Expect = 0.69 Identities = 20/71 (28%), Positives = 34/71 (47%) Frame = +2 Query: 284 MKITVKQLQGGECLIDIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQMYP 463 M I +K L G ++++ I +K + K IPV+QQ+ D T+ Y Sbjct: 86 MFIFIKTLTGRRIVLEVENCDTIENIKAKIQDKQGIPVDQQRLIFKGKQLEDGLTLADY- 144 Query: 464 NIKEGTKLNLV 496 NI+ + L+L+ Sbjct: 145 NIQNDSILHLI 155 >At5g20000.1 68418.m02380 26S proteasome AAA-ATPase subunit, putative almost identical to 26S proteasome AAA-ATPase subunit RPT6a GI:6652888 from [Arabidopsis thaliana]; almost identical to a member of conserved Sug1 CAD family AtSUG1 GI:13537115 from [Arabidopsis thaliana] Length = 419 Score = 29.9 bits (64), Expect = 1.6 Identities = 17/47 (36%), Positives = 30/47 (63%) Frame = +2 Query: 221 VLKTPGSFVTQYFKTVKIISKMKITVKQLQGGECLIDIYPSMLISEL 361 +L+ PGS+V + VK++ K K+ VK G+ ++DI S+ I++L Sbjct: 77 LLQEPGSYVGE---VVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKL 120 >At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to polyubiquitin (ubq8) GI:870793, GB:L05917 [Arabidopsis thaliana] (Genetics 139 (2), 921-939 (1995)) Length = 631 Score = 29.9 bits (64), Expect = 1.6 Identities = 20/73 (27%), Positives = 36/73 (49%) Frame = +2 Query: 284 MKITVKQLQGGECLIDIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQMYP 463 ++I K L +D+ S I +K + K IP++QQ+ D T+ Y Sbjct: 3 IQIYAKTLTEKTITLDVETSDSIHNVKAKIQNKEGIPLDQQRLIFAGKQLEDGLTLADY- 61 Query: 464 NIKEGTKLNLVVK 502 NI++ + L+LV++ Sbjct: 62 NIQKESTLHLVLR 74 >At5g19990.1 68418.m02379 26S proteasome AAA-ATPase subunit (RPT6a) Length = 419 Score = 29.1 bits (62), Expect = 2.8 Identities = 16/47 (34%), Positives = 30/47 (63%) Frame = +2 Query: 221 VLKTPGSFVTQYFKTVKIISKMKITVKQLQGGECLIDIYPSMLISEL 361 +L+ PGS+V + VK++ K K+ VK G+ ++DI S+ I+++ Sbjct: 77 LLQEPGSYVGE---VVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKI 120 >At5g54180.1 68418.m06746 mitochondrial transcription termination factor-related / mTERF-related contains Pfam profile PF02536: mTERF Length = 500 Score = 27.9 bits (59), Expect = 6.5 Identities = 12/44 (27%), Positives = 23/44 (52%) Frame = +2 Query: 521 DASFKHYKRQGMSDKDAANTANKLLRIVQEKFDKMSWDEVDRLC 652 D+ KH + + + + NKLL + E+F K + D ++ +C Sbjct: 457 DSRIKHRYEEKLKSRGENMSLNKLLTVSAERFSKAA-DNIEMIC 499 >At1g53950.1 68414.m06145 ubiquitin family protein contains INTERPRO:IPR000626 ubiquitin domain Length = 216 Score = 27.5 bits (58), Expect = 8.5 Identities = 17/42 (40%), Positives = 21/42 (50%) Frame = +2 Query: 284 MKITVKQLQGGECLIDIYPSMLISELKRHVARKLHIPVEQQK 409 M I +K L G ++ S I ELK K IPVEQQ+ Sbjct: 50 MMIFIKTLTGRTNTYEVKGSDTIRELKAKHEEKEGIPVEQQR 91 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,597,087 Number of Sequences: 28952 Number of extensions: 233234 Number of successful extensions: 540 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 531 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 540 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1412971776 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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