Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc10j14
(732 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 32 0.097
SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 32 0.097
SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 31 0.13
SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 30 0.39
SPBC3B8.09 |||U3 snoRNP-associated protein Utp3 |Schizosaccharom... 29 0.52
SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 29 0.68
SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|ch... 29 0.90
SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po... 28 1.2
SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa... 27 2.1
SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 27 2.1
SPBC13E7.02 |cwf24||GCN5-related N acetyltransferase|Schizosacch... 27 2.1
SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces pomb... 27 2.8
SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces po... 27 2.8
SPAC227.01c ||SPAPB21F2.04c|Erd1 homolog|Schizosaccharomyces pom... 27 2.8
SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces ... 27 3.6
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 26 4.8
SPAC1D4.09c |||DUF602 family protein|Schizosaccharomyces pombe|c... 26 4.8
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 25 8.4
SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi... 25 8.4
SPAC26H5.08c |bgl2||glucan 1,3-beta-glucosidase Bgl2|Schizosacch... 25 8.4
SPCC962.02c |bir1|cut17, pbh1, SPCP31B10.10c|survivin homolog|Sc... 25 8.4
SPBC17D1.02 |||diphthamide biosynthesis protein |Schizosaccharom... 25 8.4
>SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex
subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1117
Score = 31.9 bits (69), Expect = 0.097
Identities = 19/69 (27%), Positives = 33/69 (47%)
Frame = +3
Query: 510 YKNIIKVLQERSVISVENSDNNCDINMENQAKIVALEAELKNEKNHSDQVTSENRQLIEE 689
Y I+ +L+ IS E C +N ++ LEA+L+ + H D T + + +EE
Sbjct: 635 YNMILNLLRVEG-ISPEFMLERCFFQFQNSLEVPKLEAKLEESQQHYDSFTILDERPLEE 693
Query: 690 NTRLNEQVQ 716
L Q++
Sbjct: 694 YHTLKTQLE 702
>SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 504
Score = 31.9 bits (69), Expect = 0.097
Identities = 14/62 (22%), Positives = 27/62 (43%)
Frame = +3
Query: 219 KKEPVINFKFECSVCFETYSQQSNDTCPFLIPTTCDHGFCFKCVIDLQSNAMNIPHSIVC 398
K+ V++ + C +C++ + C CDH FC C + ++ S++
Sbjct: 120 KQREVVHHEGTCEICYD-------EGCLPFFSAECDHEFCLACYRQYLDSRISEGESVIQ 172
Query: 399 CP 404
CP
Sbjct: 173 CP 174
>SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 251
Score = 31.5 bits (68), Expect = 0.13
Identities = 14/58 (24%), Positives = 27/58 (46%)
Frame = +3
Query: 252 CSVCFETYSQQSNDTCPFLIPTTCDHGFCFKCVIDLQSNAMNIPHSIVCCPLCNTQVK 425
C +C + ++ T P+ T C H +C+ C++ N V CP+C +++
Sbjct: 192 CMMCVQRGDERVAITTPYT--TDCGHTYCYACIMSRLKLVNN-----VSCPICKHRIR 242
>SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 456
Score = 29.9 bits (64), Expect = 0.39
Identities = 23/88 (26%), Positives = 37/88 (42%)
Frame = +3
Query: 321 CDHGFCFKCVIDLQSNAMNIPHSIVCCPLCNTQVKMWRSLKPNAVVTCKFYKKTQERVPA 500
C H FC C+I LQ ++ CPLC + ++ ++ N + KT
Sbjct: 375 CSHVFCLHCLIILQKQKVDF------CPLCRAK-EVMKADSRNIDHALMNFMKTYFPREI 427
Query: 501 VQQYKNIIKVLQERSVISVENSDNNCDI 584
++++ S ISV + NNC I
Sbjct: 428 KEKFEENENDTFTPSSISVVSGQNNCVI 455
>SPBC3B8.09 |||U3 snoRNP-associated protein Utp3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 597
Score = 29.5 bits (63), Expect = 0.52
Identities = 17/85 (20%), Positives = 46/85 (54%), Gaps = 7/85 (8%)
Frame = +3
Query: 477 KTQERVPAVQQYKNIIKV------LQERSVISVENSDNNCDINMENQAKIVALEAELKNE 638
K +E++ A+ Y+++ Q++ + +N + ++ + ++ ++VALE+ E
Sbjct: 23 KEKEKINAINTYEDVANSEDEFYNAQDKILFDADNGEQADELELSDE-ELVALESSSDEE 81
Query: 639 KNHSDQVTSENRQLI-EENTRLNEQ 710
++++ SEN +L ++ +NE+
Sbjct: 82 DGNAEENLSENEELSGKKKDAVNEE 106
>SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 435
Score = 29.1 bits (62), Expect = 0.68
Identities = 15/38 (39%), Positives = 21/38 (55%)
Frame = +3
Query: 243 KFECSVCFETYSQQSNDTCPFLIPTTCDHGFCFKCVID 356
KF+C+VCF+ + N T F + T C H C C+ D
Sbjct: 171 KFQCNVCFDEF----NGTDCFQL-TRCGHVSCQSCLRD 203
>SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1208
Score = 28.7 bits (61), Expect = 0.90
Identities = 15/41 (36%), Positives = 26/41 (63%)
Frame = +3
Query: 609 VALEAELKNEKNHSDQVTSENRQLIEENTRLNEQVQELQRQ 731
+AL+ ELKN D ++S N+Q T+L E+++ L+R+
Sbjct: 935 LALQDELKNRNLLMDDISSYNKQ----TTKLQEKIKWLERE 971
>SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 811
Score = 28.3 bits (60), Expect = 1.2
Identities = 13/35 (37%), Positives = 20/35 (57%), Gaps = 2/35 (5%)
Frame = -1
Query: 675 VGFQ--KLLDHCDFFRSLIQLPKQLFLPDSPYLCH 577
+GFQ K+ D+F L +L ++L D Y+CH
Sbjct: 324 LGFQPYKITYSSDYFDKLYELAEELIKRDKAYVCH 358
>SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3
Brl2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 680
Score = 27.5 bits (58), Expect = 2.1
Identities = 13/35 (37%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = +3
Query: 249 ECSVC-FETYSQQSNDTCPFLIPTTCDHGFCFKCV 350
+CSVC FE + + I + C HGFC++C+
Sbjct: 626 KCSVCNFERWKDR--------IISLCGHGFCYQCI 652
>SPCC18.03 |||shuttle craft like transcriptional
regulator|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1077
Score = 27.5 bits (58), Expect = 2.1
Identities = 18/66 (27%), Positives = 30/66 (45%), Gaps = 4/66 (6%)
Frame = +3
Query: 201 RMMAELKKEPVINFKFECSVCFETYSQQSN----DTCPFLIPTTCDHGFCFKCVIDLQSN 368
RM+ ELK N +ECSVC +T + ++ TC + +C +C + +
Sbjct: 185 RMIVELK-----NRLYECSVCTDTINPSTSIWSCGTCYHVFHLSCIRKWCKNSIEQRNED 239
Query: 369 AMNIPH 386
A P+
Sbjct: 240 AWRCPY 245
>SPBC13E7.02 |cwf24||GCN5-related N
acetyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 533
Score = 27.5 bits (58), Expect = 2.1
Identities = 15/46 (32%), Positives = 20/46 (43%)
Frame = +3
Query: 216 LKKEPVINFKFECSVCFETYSQQSNDTCPFLIPTTCDHGFCFKCVI 353
+K E + F C +C + Y I TTC H FC +C I
Sbjct: 242 VKNEKKEDIPFVCLICKKDYRSP--------IATTCGHHFCEQCAI 279
>SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 372
Score = 27.1 bits (57), Expect = 2.8
Identities = 16/61 (26%), Positives = 28/61 (45%)
Frame = +3
Query: 240 FKFECSVCFETYSQQSNDTCPFLIPTTCDHGFCFKCVIDLQSNAMNIPHSIVCCPLCNTQ 419
F EC +C E++++ ++ C H F C+ ++ H+ CP CNT+
Sbjct: 317 FGVECVICLESFTKGDK-----VVALPCKHEFHRPCIAKW---IVDYRHA---CPTCNTE 365
Query: 420 V 422
V
Sbjct: 366 V 366
>SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 639
Score = 27.1 bits (57), Expect = 2.8
Identities = 15/51 (29%), Positives = 28/51 (54%)
Frame = +3
Query: 573 NCDINMENQAKIVALEAELKNEKNHSDQVTSENRQLIEENTRLNEQVQELQ 725
NC + + NQ + + + NH D V ++ + +++EN RL EQ+ L+
Sbjct: 583 NCGLLLPNQVSMPNISNIVST--NHLD-VETQMKLIMDENQRLREQIAVLR 630
>SPAC227.01c ||SPAPB21F2.04c|Erd1 homolog|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 373
Score = 27.1 bits (57), Expect = 2.8
Identities = 16/39 (41%), Positives = 21/39 (53%)
Frame = -2
Query: 656 LITVIFFVL*FSFQSNYFCLILHIYVTIVVAVFDTNHAP 540
L+ +FF++ F S F LILH+ V V VF H P
Sbjct: 16 LVLRLFFLVVFGLYS--FTLILHLLVINRVDVFSLLHTP 52
>SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 817
Score = 26.6 bits (56), Expect = 3.6
Identities = 17/80 (21%), Positives = 38/80 (47%), Gaps = 2/80 (2%)
Frame = +3
Query: 498 AVQQYKNIIKVLQERSVISVENSDNNCDINMENQAKIVALEAELKNEKNHSDQ--VTSEN 671
A + + + IK L + + + D ++ + +L + K+ ++ + S
Sbjct: 303 AEEHFSHKIKELTSENELKISRLQEEKDSLLKKVQEGASLAMQRVQNKHDLEKKRLQSAI 362
Query: 672 RQLIEENTRLNEQVQELQRQ 731
+ L EEN L +Q+++LQR+
Sbjct: 363 QPLQEENNSLKQQIEQLQRE 382
>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 468
Score = 26.2 bits (55), Expect = 4.8
Identities = 12/45 (26%), Positives = 22/45 (48%)
Frame = +3
Query: 249 ECSVCFETYSQQSNDTCPFLIPTTCDHGFCFKCVIDLQSNAMNIP 383
EC +C E + PF T C H +C++C+++ + + P
Sbjct: 84 ECPICTEALQR------PFT--THCGHTYCYECLLNWLKESKSCP 120
>SPAC1D4.09c |||DUF602 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 240
Score = 26.2 bits (55), Expect = 4.8
Identities = 12/39 (30%), Positives = 19/39 (48%)
Frame = -2
Query: 143 IAPLQSVFQLSVEIFKRRTFIWRRPVAAAIVGVPWHFAY 27
I L+ V QL VE+ +W P+ ++ + FAY
Sbjct: 82 IKSLKDVVQLQVELDDSGKVLWLCPITRHVMSDTYQFAY 120
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 25.4 bits (53), Expect = 8.4
Identities = 16/55 (29%), Positives = 31/55 (56%)
Frame = +3
Query: 558 ENSDNNCDINMENQAKIVALEAELKNEKNHSDQVTSENRQLIEENTRLNEQVQEL 722
E++ N D++ E++A+ LE+++ S +EN EEN L+E+V ++
Sbjct: 3940 EDTANQSDLD-ESEAR--ELESDMNGVTKDSVVSENENSDSEEENQDLDEEVNDI 3991
>SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 25.4 bits (53), Expect = 8.4
Identities = 19/66 (28%), Positives = 26/66 (39%), Gaps = 1/66 (1%)
Frame = +3
Query: 189 IKQQRMMAELKKEPVI-NFKFECSVCFETYSQQSNDTCPFLIPTTCDHGFCFKCVIDLQS 365
I +R + + K P I +CS+C E CP T C H FC+ C+ S
Sbjct: 234 ITDERDLEDKNKLPFIPEGNRKCSLCMEFIH------CP--AATECGHIFCWSCINGWTS 285
Query: 366 NAMNIP 383
P
Sbjct: 286 KKSECP 291
>SPAC26H5.08c |bgl2||glucan 1,3-beta-glucosidase
Bgl2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 321
Score = 25.4 bits (53), Expect = 8.4
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = -1
Query: 270 FRNTHCTQI*N*LPALSLIPPSFAAVLFVW 181
+ + C + LPAL+ P +F+A+L VW
Sbjct: 78 YATSDCNTLEYLLPALAQSPYNFSAILGVW 107
>SPCC962.02c |bir1|cut17, pbh1, SPCP31B10.10c|survivin
homolog|Schizosaccharomyces pombe|chr 3|||Manual
Length = 997
Score = 25.4 bits (53), Expect = 8.4
Identities = 15/63 (23%), Positives = 35/63 (55%), Gaps = 3/63 (4%)
Frame = +3
Query: 477 KTQERVPAVQQYKNIIKVLQERSVI--SVENSDNNCDINMENQAKI-VALEAELKNEKNH 647
+T ++P ++ + +VLQ ++++ S E+S + ++Q I V+ +L+N+
Sbjct: 527 ETNLKIPIPEKKRKFQEVLQSKNILVSSTEDSHEPVKVTEDSQTAIHVSKFEDLENKSME 586
Query: 648 SDQ 656
S+Q
Sbjct: 587 SEQ 589
>SPBC17D1.02 |||diphthamide biosynthesis protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 503
Score = 25.4 bits (53), Expect = 8.4
Identities = 12/43 (27%), Positives = 24/43 (55%)
Frame = -3
Query: 328 WSHVVGIKKGQVSLDC*EYVSKHTLHSNLKLITGSFFNSAIIR 200
+ V+ + +G+ S + E +++ + + LITG F NS +R
Sbjct: 373 FDEVLKLSEGKQSKEPSEVLTEESAEPHFSLITGKFVNSTPMR 415
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,966,659
Number of Sequences: 5004
Number of extensions: 60609
Number of successful extensions: 237
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 226
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 237
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 345237368
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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