BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmnc10i24 (762 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces po... 48 2e-06 SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Sc... 37 0.004 SPBC23E6.01c ||SPBPJ758.01|RNA-binding protein, rrm type|Schizos... 37 0.004 SPBP16F5.06 |||ribosome biogenesis protein Nop6|Schizosaccharomy... 36 0.006 SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces... 35 0.015 SPAC2F7.11 |nrd1|msa2|RNA-binding protein Nrd1|Schizosaccharomyc... 33 0.059 SPBC1289.12 |usp109||U1 snRNP-associated protein Usp109|Schizosa... 32 0.078 SPBC660.15 |||mRNA cleavage factor complex subunit |Schizosaccha... 32 0.078 SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomy... 32 0.078 SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 32 0.10 SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 31 0.14 SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating tra... 30 0.31 SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual 29 0.72 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 29 0.72 SPBC660.11 |tcg1|mug187|single-stranded telomeric binding protei... 29 0.72 SPAC3A11.03 |||methyltransferase |Schizosaccharomyces pombe|chr ... 27 2.9 SPAC57A7.04c |pabp||mRNA export shuttling protein |Schizosacchar... 27 3.9 SPBC365.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 8.9 SPAC9G1.05 |||actin cortical patch component Aip1 |Schizosacchar... 25 8.9 SPBC11C11.04c |alp1||tubulin specific chaperone cofactor D |Schi... 25 8.9 >SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 365 Score = 47.6 bits (108), Expect = 2e-06 Identities = 21/68 (30%), Positives = 35/68 (51%) Frame = +1 Query: 127 QTKVFVGSLPQGSKPEDLRKLFERFGVVTECDIMNRCGFVHMQTEEQAAAAIRALHNSTF 306 +T++FVG +P + ED+ F+ +G + +C +MN GFV ++ A + F Sbjct: 3 ETRLFVGRIPPQATREDMMDFFKGYGQILDCKLMNGFGFVEVEDARDARDIVNDFQGKEF 62 Query: 307 NGGVISVE 330 G I VE Sbjct: 63 MGSRIVVE 70 >SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Schizosaccharomyces pombe|chr 1|||Manual Length = 434 Score = 36.7 bits (81), Expect = 0.004 Identities = 20/74 (27%), Positives = 37/74 (50%), Gaps = 7/74 (9%) Frame = +1 Query: 127 QTKVFVGSLPQGSKPEDLRKLFERFGVVTECDIMN-------RCGFVHMQTEEQAAAAIR 285 + +V+VG+L + +L++ + G V C+I+N C + T E+A AI+ Sbjct: 65 ERRVYVGNLSYQVRWFELKEFMGQVGNVLNCEILNLPNGLSKGCAIIEYSTAEEARTAIK 124 Query: 286 ALHNSTFNGGVISV 327 L N F G ++ + Sbjct: 125 TLSNQKFMGRLVYI 138 Score = 26.6 bits (56), Expect = 3.9 Identities = 18/66 (27%), Positives = 28/66 (42%), Gaps = 7/66 (10%) Frame = +1 Query: 136 VFVGSLPQGSKPEDLRKLFERFGVVTECDI-------MNRCGFVHMQTEEQAAAAIRALH 294 ++VG+LP + +L LF G V I G V + E AA++I L+ Sbjct: 282 IYVGNLPWATSDRNLLDLFTDIGSVIRARIAYEPTGRSKGFGVVQFENENDAASSIEKLN 341 Query: 295 NSTFNG 312 + G Sbjct: 342 GYRYGG 347 >SPBC23E6.01c ||SPBPJ758.01|RNA-binding protein, rrm type|Schizosaccharomyces pombe|chr 2|||Manual Length = 473 Score = 36.7 bits (81), Expect = 0.004 Identities = 31/127 (24%), Positives = 40/127 (31%), Gaps = 3/127 (2%) Frame = +1 Query: 136 VFVGSLPQGSKPEDLRKLFERFGVVTECDIM--NRCGFVHMQTEEQAAAAIRALHNSTFN 309 VFVG L + E+L+ LF+ FG + I CGFV + A AI L Sbjct: 305 VFVGGLSKFVSEEELKYLFQNFGEIVYVKIPPGKGCGFVQFVNRQSAEIAINQLQGYPLG 364 Query: 310 GGVISVEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVRHAPPPRD-APYMRDRPGPVR 486 I + P PP +PY P P+ Sbjct: 365 NSRIRLSWGRNQNPIAAPALNYQSQVSQTTIPATSLFPAMSLPPQAQFSPYPAVAPSPLA 424 Query: 487 GYERGPP 507 RG P Sbjct: 425 LQTRGAP 431 >SPBP16F5.06 |||ribosome biogenesis protein Nop6|Schizosaccharomyces pombe|chr 2|||Manual Length = 478 Score = 35.9 bits (79), Expect = 0.006 Identities = 21/78 (26%), Positives = 39/78 (50%), Gaps = 10/78 (12%) Frame = +1 Query: 127 QTKVFVGSLPQGSKPEDLRKLFERFGVVTECDIMN---------RCGFVHMQTEEQAAAA 279 + +++VG L + DL F RFG V+ +I+N R +++ +T+E Sbjct: 4 EKRIYVGGLSSSIESSDLESRFSRFGSVSNLEIINKSTPVGTIQRFAYLNFRTDEDKWQK 63 Query: 280 IRA-LHNSTFNGGVISVE 330 ++ L N+TF G + +E Sbjct: 64 CKSYLSNATFKGSKLRIE 81 >SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces pombe|chr 1|||Manual Length = 609 Score = 34.7 bits (76), Expect = 0.015 Identities = 22/81 (27%), Positives = 40/81 (49%), Gaps = 13/81 (16%) Frame = +1 Query: 127 QTKVFVGSLPQGSKPEDLRKLFERFGVVTECDIMNR-------------CGFVHMQTEEQ 267 + ++VG+LP + DL +LFE FG ++ +++R F+ + + Sbjct: 19 EISIYVGNLPSTCQSSDLHELFEPFGNFSKFHMLSRKKNKSTDSKSPTLFAFITFENKCS 78 Query: 268 AAAAIRALHNSTFNGGVISVE 330 A AI +L+ S+F G + VE Sbjct: 79 ADNAIASLNGSSFQGNTLKVE 99 >SPAC2F7.11 |nrd1|msa2|RNA-binding protein Nrd1|Schizosaccharomyces pombe|chr 1|||Manual Length = 529 Score = 32.7 bits (71), Expect = 0.059 Identities = 20/60 (33%), Positives = 31/60 (51%), Gaps = 2/60 (3%) Frame = +1 Query: 136 VFVGSLPQGSKPEDLRKLFERFGVVTECDIM--NRCGFVHMQTEEQAAAAIRALHNSTFN 309 VF+G+LP G +++R+ E FG + + I+ FVH AAAI+A+ N Sbjct: 208 VFLGNLPNGITEDEIREDLEPFGPIDQIKIVTERNIAFVHFL---NIAAAIKAVQELPLN 264 >SPBC1289.12 |usp109||U1 snRNP-associated protein Usp109|Schizosaccharomyces pombe|chr 2|||Manual Length = 352 Score = 32.3 bits (70), Expect = 0.078 Identities = 17/61 (27%), Positives = 27/61 (44%) Frame = +1 Query: 130 TKVFVGSLPQGSKPEDLRKLFERFGVVTECDIMNRCGFVHMQTEEQAAAAIRALHNSTFN 309 T V+V LP+ ++LR F FG + + N G + A AI ++N + Sbjct: 176 TAVYVHQLPENITTQELRSYFLHFGEILYTQVNNNSGRIVFAQRYFAEQAINEMNNFPLH 235 Query: 310 G 312 G Sbjct: 236 G 236 >SPBC660.15 |||mRNA cleavage factor complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 474 Score = 32.3 bits (70), Expect = 0.078 Identities = 13/32 (40%), Positives = 20/32 (62%) Frame = +1 Query: 133 KVFVGSLPQGSKPEDLRKLFERFGVVTECDIM 228 K+F+G L + + LR FE+FG V +C +M Sbjct: 164 KMFIGGLNWETTDDSLRDYFEQFGEVLDCTVM 195 Score = 29.5 bits (63), Expect = 0.55 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +1 Query: 133 KVFVGSLPQGSKPEDLRKLFERFGVVTECDIM 228 K+FVG +P E+ R F +FG V + +M Sbjct: 248 KMFVGGVPGDCTEEEFRNFFNQFGRVLDATLM 279 >SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomyces pombe|chr 3|||Manual Length = 654 Score = 32.3 bits (70), Expect = 0.078 Identities = 15/54 (27%), Positives = 27/54 (50%), Gaps = 2/54 (3%) Frame = +1 Query: 136 VFVGSLPQGSKPEDLRKLFERFGVVTECDIMNR--CGFVHMQTEEQAAAAIRAL 291 VF+G+LP +++ + F +FG + I+++ FVH A +R L Sbjct: 301 VFIGNLPSSYHEKEIEEAFGKFGKIEHIKILSKKNIAFVHFLNIRDAIKVVRTL 354 >SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 31.9 bits (69), Expect = 0.10 Identities = 23/67 (34%), Positives = 33/67 (49%), Gaps = 3/67 (4%) Frame = +1 Query: 115 IMVPQTKVFVGSLPQGSKPEDLRKLFERFGVVTECDIMN---RCGFVHMQTEEQAAAAIR 285 I V +F+G + + + LR +FERFG V I+N R GF+ M + A A Sbjct: 401 IKVYSRTLFLGGITRSVREPVLRSMFERFGSVQSL-ILNHNYRHGFLKMFRRDAAEKAQV 459 Query: 286 ALHNSTF 306 A+ N F Sbjct: 460 AMENVPF 466 >SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 674 Score = 31.5 bits (68), Expect = 0.14 Identities = 23/83 (27%), Positives = 42/83 (50%), Gaps = 8/83 (9%) Frame = +1 Query: 106 KSVIMVPQTKVFVGSLPQG-SKPEDLRKLFERFGVVTECDIMNR-----CG--FVHMQTE 261 K++ + ++ + +LP KP+ L F +FG V E I + CG FV M+ Sbjct: 99 KTIRQDNRPRLIIRNLPWSIKKPQHLEPHFSKFGKVREIKIPTKGGGRMCGFAFVWMKDR 158 Query: 262 EQAAAAIRALHNSTFNGGVISVE 330 + A A+ +L+ + +G I+V+ Sbjct: 159 KAAEEAMNSLNGTEIDGRPIAVD 181 >SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating transcription Rct1|Schizosaccharomyces pombe|chr 2|||Manual Length = 432 Score = 30.3 bits (65), Expect = 0.31 Identities = 12/35 (34%), Positives = 20/35 (57%) Frame = +1 Query: 124 PQTKVFVGSLPQGSKPEDLRKLFERFGVVTECDIM 228 P+ +FV L ++ EDL +F RFG + C ++ Sbjct: 242 PENVLFVCKLNPVTQDEDLELIFSRFGKIISCQVI 276 >SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual Length = 738 Score = 29.1 bits (62), Expect = 0.72 Identities = 18/68 (26%), Positives = 33/68 (48%), Gaps = 1/68 (1%) Frame = +1 Query: 130 TKVFVGSLPQGS-KPEDLRKLFERFGVVTECDIMNRCGFVHMQTEEQAAAAIRALHNSTF 306 +++F+G L S +L K+F+ +G + + + GFV T E A A+ A + Sbjct: 363 SRLFLGHLNTKSLSKRNLWKVFKIYGPLAQIVLKANYGFVQFFTNEDCARALNAEQGNFV 422 Query: 307 NGGVISVE 330 G + +E Sbjct: 423 RGQKLHLE 430 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 29.1 bits (62), Expect = 0.72 Identities = 13/65 (20%), Positives = 30/65 (46%) Frame = -3 Query: 295 YEARESPLPLVPLSAYARTRTGSLYHTL*RRRNAQTAFLGPPVWSPAAGCRRTPSFAAP* 116 YE ++ + ++ +R R G + + + +N A +G + + P F+ P Sbjct: 285 YEVQQVGIMYPDMTEVSRLRAGQVGYIIWNMKNIDEAIVGDTYSTIGSNVEALPGFSIPQ 344 Query: 115 SLIYI 101 S++Y+ Sbjct: 345 SMVYV 349 >SPBC660.11 |tcg1|mug187|single-stranded telomeric binding protein Tgc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 348 Score = 29.1 bits (62), Expect = 0.72 Identities = 13/31 (41%), Positives = 18/31 (58%) Frame = +1 Query: 133 KVFVGSLPQGSKPEDLRKLFERFGVVTECDI 225 +VFVG L +K ++R LFE G V + I Sbjct: 45 RVFVGRLSTSTKKSEIRSLFETVGTVRKVTI 75 >SPAC3A11.03 |||methyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 247 Score = 27.1 bits (57), Expect = 2.9 Identities = 13/38 (34%), Positives = 19/38 (50%) Frame = +3 Query: 456 IHEGPARSGARLRTRSAGGAL*RALCVRRGPAWVRADG 569 I++ P +SG R+ AG L LC + G + DG Sbjct: 163 IYQHPVQSGMRVLELGAGTGLVSILCAKMGSIVLATDG 200 >SPAC57A7.04c |pabp||mRNA export shuttling protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 653 Score = 26.6 bits (56), Expect = 3.9 Identities = 18/65 (27%), Positives = 26/65 (40%), Gaps = 7/65 (10%) Frame = +1 Query: 136 VFVGSLPQGSKPEDLRKLFERFGVVTECDIM-------NRCGFVHMQTEEQAAAAIRALH 294 VF+ +L + L F FG + C + GFVH + E A AAI ++ Sbjct: 170 VFIKNLDPAIDNKALHDTFSAFGKILSCKVAVDELGNAKGYGFVHFDSVESANAAIEHVN 229 Query: 295 NSTFN 309 N Sbjct: 230 GMLLN 234 >SPBC365.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 233 Score = 25.4 bits (53), Expect = 8.9 Identities = 12/25 (48%), Positives = 17/25 (68%) Frame = +1 Query: 136 VFVGSLPQGSKPEDLRKLFERFGVV 210 +FVG+LP+ S E L+ F+R G V Sbjct: 94 LFVGNLPKDSSVETLQLHFKRAGQV 118 >SPAC9G1.05 |||actin cortical patch component Aip1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 595 Score = 25.4 bits (53), Expect = 8.9 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +2 Query: 299 LHLTAASYPWSAVASKNAGSVGAVAVDEV 385 +H+ + P +A KNA S+GA V+ V Sbjct: 546 IHIYSVERPMKYIAMKNAHSLGATQVEWV 574 >SPBC11C11.04c |alp1||tubulin specific chaperone cofactor D |Schizosaccharomyces pombe|chr 2|||Manual Length = 1107 Score = 25.4 bits (53), Expect = 8.9 Identities = 9/22 (40%), Positives = 12/22 (54%) Frame = -3 Query: 439 GARVARGRLRDAPCHRAWNLVH 374 G RV +RDA C+ W+ H Sbjct: 421 GTRVTGQSIRDASCYFVWSFYH 442 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,582,403 Number of Sequences: 5004 Number of extensions: 46884 Number of successful extensions: 144 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 137 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 144 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 365309308 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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