SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc10i20
         (739 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces ...    28   1.6  
SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc...    27   2.1  
SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces p...    27   2.1  
SPBC23G7.11 |||DNA-3-methyladenine glycosidase Mag2 |Schizosacch...    27   3.7  
SPCC1235.03 |||SMR and CUE domain protein|Schizosaccharomyces po...    27   3.7  
SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces pom...    26   4.9  
SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb...    26   6.4  
SPCC1682.06 |||sequence orphan|Schizosaccharomyces pombe|chr 3||...    26   6.4  
SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||...    25   8.5  
SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa...    25   8.5  
SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe...    25   8.5  

>SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1147

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 20/60 (33%), Positives = 28/60 (46%)
 Frame = +3

Query: 480 DVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDCENARRETAQLAN 659
           D  +KID LT+KL VAN   AE N +L   A E +   +  + +        +E  Q  N
Sbjct: 618 DSASKIDSLTEKLKVAN---AEKNAALCEAALEKVPLAKHNKLSDGTFSTPDKENVQSTN 674


>SPBC21.01 |mis17|SPBC776.19|kinetochore protein
           Mis17|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 441

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 17/46 (36%), Positives = 24/46 (52%), Gaps = 4/46 (8%)
 Frame = +3

Query: 534 DLAEANRSLILF----ANEMIVARRDAETARQDCENARRETAQLAN 659
           DL +ANR L       ANE +  +RD    R+D  + +R+  QL N
Sbjct: 318 DLLDANRILSTACKKAANEKLAVQRDLSKLREDRLSVQRKKIQLRN 363


>SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 728

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 9/27 (33%), Positives = 19/27 (70%)
 Frame = +3

Query: 333 LITKSGVIQLIMKSKLPYAIELQEWLL 413
           L+ K GV+ L+ K KLP+++  ++ ++
Sbjct: 542 LLVKDGVVHLVDKVKLPFSVSQKDMII 568


>SPBC23G7.11 |||DNA-3-methyladenine glycosidase Mag2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 213

 Score = 26.6 bits (56), Expect = 3.7
 Identities = 17/52 (32%), Positives = 21/52 (40%)
 Frame = +3

Query: 225 DGKYKSTFEHADQIQHHAPDSVAKQGDPLYLHPHTVLITKSGVIQLIMKSKL 380
           D  YK   +H   I +     V K G P  L PH       G+I+ I   KL
Sbjct: 4   DSDYKRAEKHLSSIDNKWSSLVKKVG-PCTLTPHPEHAPYEGIIRAITSQKL 54


>SPCC1235.03 |||SMR and CUE domain protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 399

 Score = 26.6 bits (56), Expect = 3.7
 Identities = 14/75 (18%), Positives = 36/75 (48%)
 Frame = +3

Query: 477 NDVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDCENARRETAQLA 656
           ND++   D+L     +  ++  E     ++ +N    +R+  +  ++   N+R+  ++  
Sbjct: 19  NDLVRSSDELLNHRAIQESEQIETAADALITSNIGHRSRQRKKKTKK-ATNSRKPLSKFQ 77

Query: 657 NRMADIAQDVIAKPS 701
           +   ++ +D I KPS
Sbjct: 78  SNTEEVNEDPILKPS 92


>SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 437

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 5/53 (9%)
 Frame = -1

Query: 562 MSDRLASAKSALATVNFCVRSSILAI-----TSFVSISTAGAYLPVHST*GMT 419
           + DRL SA+S   T   C+ ++ LA      T +VS  T G +  V S  G+T
Sbjct: 128 IKDRLVSAQSISGTGALCIAANFLASFYPSKTIYVSDPTWGNHKNVFSRAGLT 180


>SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 262

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 14/37 (37%), Positives = 20/37 (54%)
 Frame = +1

Query: 196 IVNGQYVSTSTASTNRRLSMPTKSSTMLQIAWQSRAT 306
           I  G Y STS +ST+   + P+ SST    +  S +T
Sbjct: 129 ISGGIYSSTSASSTSSSTATPSSSSTTSSSSSSSSST 165


>SPCC1682.06 |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 238

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 18/62 (29%), Positives = 27/62 (43%)
 Frame = +3

Query: 450 YAPAVEMDTNDVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDCEN 629
           YAP+V      +   I  L + +  A   LA    SL+LF   + V  +D ET   +  +
Sbjct: 146 YAPSVAKANEGLAKAIAGLAKYVAKAIQGLAHIILSLLLFILGLEVIEQDEETGDVEMSS 205

Query: 630 AR 635
            R
Sbjct: 206 MR 207


>SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1116

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 16/59 (27%), Positives = 29/59 (49%)
 Frame = +3

Query: 138  DEQPVRFVAKDIASSLKYVNCERAIRVHVDGKYKSTFEHADQIQHHAPDSVAKQGDPLY 314
            +EQ VRFV++ +   L Y++ +  I  H D K  +     D +   +   ++K  D +Y
Sbjct: 928  EEQLVRFVSRQVLYGLSYLHSKGII--HRDLKADNLLIDFDGVCKISDFGISKHSDNVY 984


>SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 669

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 14/42 (33%), Positives = 20/42 (47%)
 Frame = +3

Query: 135 GDEQPVRFVAKDIASSLKYVNCERAIRVHVDGKYKSTFEHAD 260
           GDE     V+ D  S    V+  +   ++  GKY+ TF H D
Sbjct: 48  GDEIECIVVSMDDKSKKARVSLRQEDILNALGKYEETFRHVD 89


>SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 716

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 9/21 (42%), Positives = 17/21 (80%)
 Frame = +3

Query: 564 LFANEMIVARRDAETARQDCE 626
           +FANE+++ + DA++ +QD E
Sbjct: 556 IFANEILIEQADAQSFKQDEE 576


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,202,924
Number of Sequences: 5004
Number of extensions: 67898
Number of successful extensions: 210
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 202
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -