BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmnc10i20 (739 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces ... 28 1.6 SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc... 27 2.1 SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces p... 27 2.1 SPBC23G7.11 |||DNA-3-methyladenine glycosidase Mag2 |Schizosacch... 27 3.7 SPCC1235.03 |||SMR and CUE domain protein|Schizosaccharomyces po... 27 3.7 SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces pom... 26 4.9 SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 26 6.4 SPCC1682.06 |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 26 6.4 SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||... 25 8.5 SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa... 25 8.5 SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe... 25 8.5 >SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1147 Score = 27.9 bits (59), Expect = 1.6 Identities = 20/60 (33%), Positives = 28/60 (46%) Frame = +3 Query: 480 DVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDCENARRETAQLAN 659 D +KID LT+KL VAN AE N +L A E + + + + +E Q N Sbjct: 618 DSASKIDSLTEKLKVAN---AEKNAALCEAALEKVPLAKHNKLSDGTFSTPDKENVQSTN 674 >SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosaccharomyces pombe|chr 2|||Manual Length = 441 Score = 27.5 bits (58), Expect = 2.1 Identities = 17/46 (36%), Positives = 24/46 (52%), Gaps = 4/46 (8%) Frame = +3 Query: 534 DLAEANRSLILF----ANEMIVARRDAETARQDCENARRETAQLAN 659 DL +ANR L ANE + +RD R+D + +R+ QL N Sbjct: 318 DLLDANRILSTACKKAANEKLAVQRDLSKLREDRLSVQRKKIQLRN 363 >SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 728 Score = 27.5 bits (58), Expect = 2.1 Identities = 9/27 (33%), Positives = 19/27 (70%) Frame = +3 Query: 333 LITKSGVIQLIMKSKLPYAIELQEWLL 413 L+ K GV+ L+ K KLP+++ ++ ++ Sbjct: 542 LLVKDGVVHLVDKVKLPFSVSQKDMII 568 >SPBC23G7.11 |||DNA-3-methyladenine glycosidase Mag2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 213 Score = 26.6 bits (56), Expect = 3.7 Identities = 17/52 (32%), Positives = 21/52 (40%) Frame = +3 Query: 225 DGKYKSTFEHADQIQHHAPDSVAKQGDPLYLHPHTVLITKSGVIQLIMKSKL 380 D YK +H I + V K G P L PH G+I+ I KL Sbjct: 4 DSDYKRAEKHLSSIDNKWSSLVKKVG-PCTLTPHPEHAPYEGIIRAITSQKL 54 >SPCC1235.03 |||SMR and CUE domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 399 Score = 26.6 bits (56), Expect = 3.7 Identities = 14/75 (18%), Positives = 36/75 (48%) Frame = +3 Query: 477 NDVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDCENARRETAQLA 656 ND++ D+L + ++ E ++ +N +R+ + ++ N+R+ ++ Sbjct: 19 NDLVRSSDELLNHRAIQESEQIETAADALITSNIGHRSRQRKKKTKK-ATNSRKPLSKFQ 77 Query: 657 NRMADIAQDVIAKPS 701 + ++ +D I KPS Sbjct: 78 SNTEEVNEDPILKPS 92 >SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 437 Score = 26.2 bits (55), Expect = 4.9 Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 5/53 (9%) Frame = -1 Query: 562 MSDRLASAKSALATVNFCVRSSILAI-----TSFVSISTAGAYLPVHST*GMT 419 + DRL SA+S T C+ ++ LA T +VS T G + V S G+T Sbjct: 128 IKDRLVSAQSISGTGALCIAANFLASFYPSKTIYVSDPTWGNHKNVFSRAGLT 180 >SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 262 Score = 25.8 bits (54), Expect = 6.4 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = +1 Query: 196 IVNGQYVSTSTASTNRRLSMPTKSSTMLQIAWQSRAT 306 I G Y STS +ST+ + P+ SST + S +T Sbjct: 129 ISGGIYSSTSASSTSSSTATPSSSSTTSSSSSSSSST 165 >SPCC1682.06 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 238 Score = 25.8 bits (54), Expect = 6.4 Identities = 18/62 (29%), Positives = 27/62 (43%) Frame = +3 Query: 450 YAPAVEMDTNDVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDCEN 629 YAP+V + I L + + A LA SL+LF + V +D ET + + Sbjct: 146 YAPSVAKANEGLAKAIAGLAKYVAKAIQGLAHIILSLLLFILGLEVIEQDEETGDVEMSS 205 Query: 630 AR 635 R Sbjct: 206 MR 207 >SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1116 Score = 25.4 bits (53), Expect = 8.5 Identities = 16/59 (27%), Positives = 29/59 (49%) Frame = +3 Query: 138 DEQPVRFVAKDIASSLKYVNCERAIRVHVDGKYKSTFEHADQIQHHAPDSVAKQGDPLY 314 +EQ VRFV++ + L Y++ + I H D K + D + + ++K D +Y Sbjct: 928 EEQLVRFVSRQVLYGLSYLHSKGII--HRDLKADNLLIDFDGVCKISDFGISKHSDNVY 984 >SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 669 Score = 25.4 bits (53), Expect = 8.5 Identities = 14/42 (33%), Positives = 20/42 (47%) Frame = +3 Query: 135 GDEQPVRFVAKDIASSLKYVNCERAIRVHVDGKYKSTFEHAD 260 GDE V+ D S V+ + ++ GKY+ TF H D Sbjct: 48 GDEIECIVVSMDDKSKKARVSLRQEDILNALGKYEETFRHVD 89 >SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe|chr 1|||Manual Length = 716 Score = 25.4 bits (53), Expect = 8.5 Identities = 9/21 (42%), Positives = 17/21 (80%) Frame = +3 Query: 564 LFANEMIVARRDAETARQDCE 626 +FANE+++ + DA++ +QD E Sbjct: 556 IFANEILIEQADAQSFKQDEE 576 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,202,924 Number of Sequences: 5004 Number of extensions: 67898 Number of successful extensions: 210 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 202 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 210 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 349251756 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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