SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc10i20
         (739 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g37060.1 68417.m05248 patatin, putative similar to patatin-li...    33   0.20 
At5g35695.1 68418.m04268 hypothetical protein                          31   0.80 
At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain...    29   4.3  
At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain...    29   4.3  
At5g64070.1 68418.m08046 phosphatidylinositol 4-kinase (PI4K) ne...    27   9.8  
At2g26340.1 68415.m03160 expressed protein                             27   9.8  

>At4g37060.1 68417.m05248 patatin, putative similar to patatin-like
           latex allergen [Hevea brasiliensis][PMID:10589016];
           contains patatin domain PF01734
          Length = 414

 Score = 33.1 bits (72), Expect = 0.20
 Identities = 28/84 (33%), Positives = 38/84 (45%), Gaps = 4/84 (4%)
 Frame = +3

Query: 243 TFEHADQIQHHAPDSVAK--QGDPLYLH-PHTVLITKSGVIQLIMKSKLPYAIELQEWLL 413
           TFE +  I H+    V K  Q +  YL      L   +  + L  KS L   I+L E +L
Sbjct: 295 TFESSRDIVHYHSSVVFKALQSEDKYLRIDDDTLEGDASTLDLSTKSNLENLIKLGEKML 354

Query: 414 EEVIPQV-LCTGKYAPAVEMDTND 482
              + Q+ + TG Y PA E   ND
Sbjct: 355 TNRVMQMNIDTGTYEPAAENINND 378


>At5g35695.1 68418.m04268 hypothetical protein
          Length = 211

 Score = 31.1 bits (67), Expect = 0.80
 Identities = 21/61 (34%), Positives = 27/61 (44%)
 Frame = -3

Query: 686 HVLRNVRHAVGQLRRFAARVFAILPSRFSVASSHNHFVGKQNERSVGFRQICVGHRQFLR 507
           H L N+RH    LR    R+F I  SRF++  S   F  K   +  G    C     FLR
Sbjct: 87  HELFNLRHV--SLRNVIERIFGIFKSRFAIFKSAPPFSYK---KQAGLVLTCAALHNFLR 141

Query: 506 Q 504
           +
Sbjct: 142 K 142


>At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing
           protein / RhoGAP domain-containing protein weak
           similarity to rho-GTPase activating protein [Homo
           sapiens] GI:14245732; contains Pfam profiles PF00169: PH
           domain, PF00620: RhoGAP domain
          Length = 870

 Score = 28.7 bits (61), Expect = 4.3
 Identities = 17/47 (36%), Positives = 22/47 (46%)
 Frame = +3

Query: 75  QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 215
           Q   G F    DT   +Y+       RF+ +D  S+L YVN ER  R
Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735


>At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing
           protein / RhoGAP domain-containing protein weak
           similarity to rho-GTPase activating protein [Homo
           sapiens] GI:14245732; contains Pfam profiles PF00169: PH
           domain, PF00620: RhoGAP domain
          Length = 822

 Score = 28.7 bits (61), Expect = 4.3
 Identities = 17/47 (36%), Positives = 22/47 (46%)
 Frame = +3

Query: 75  QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 215
           Q   G F    DT   +Y+       RF+ +D  S+L YVN ER  R
Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735


>At5g64070.1 68418.m08046 phosphatidylinositol 4-kinase (PI4K)
           nearly identical to gi:4467359
          Length = 1121

 Score = 27.5 bits (58), Expect = 9.8
 Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
 Frame = +3

Query: 249 EH-ADQIQHHAPDSVAKQGDPLYLHPHTVLITKS 347
           EH A Q+  H  D   + G PL+L P+ VL+T S
Sbjct: 872 EHLAVQLISHFYDIFQEAGLPLWLRPYEVLVTSS 905


>At2g26340.1 68415.m03160 expressed protein
          Length = 230

 Score = 27.5 bits (58), Expect = 9.8
 Identities = 19/54 (35%), Positives = 27/54 (50%)
 Frame = +3

Query: 345 SGVIQLIMKSKLPYAIELQEWLLEEVIPQVLCTGKYAPAVEMDTNDVIAKIDDL 506
           S    ++MKS+L  A+   + LL+ V  QVL  GK    V    ++  A  DDL
Sbjct: 165 SNATVVLMKSQLGTALTALDSLLQTVPSQVLDKGKAMVEVYRSASEEDAGSDDL 218


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,373,606
Number of Sequences: 28952
Number of extensions: 387542
Number of successful extensions: 1048
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1013
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1048
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -