Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc10i16
         (455 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g55220.1 68416.m06133 splicing factor, putative contains CPSF...   176   5e-45
At3g55200.1 68416.m06131 splicing factor, putative contains CPSF...   176   5e-45
At1g35240.1 68414.m04370 transcriptional factor B3 family protei...    27   4.5  
At5g51120.1 68418.m06339 polyadenylate-binding protein, putative...    27   6.0  
At5g35560.1 68418.m04231 DENN (AEX-3) domain-containing protein ...    27   7.9  
At2g41710.2 68415.m05155 ovule development protein, putative sim...    27   7.9  
At2g41710.1 68415.m05154 ovule development protein, putative sim...    27   7.9  
At1g35520.1 68414.m04410 transcriptional factor B3 family protei...    27   7.9  

>At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A
            subunit region (PF03178); contains weak WD-40 repeat
            (PF00400); similar to Splicing factor 3B subunit 3
            (SF3b130)/spliceosomal protein/Splicing factor 3B subunit
            3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
            EMBL:HSAJ1443_1
          Length = 1214

 Score =  176 bits (429), Expect = 5e-45
 Identities = 77/123 (62%), Positives = 99/123 (80%)
 Frame = +1

Query: 1    VVNFHVGETVTSLQRATLIPGGSEALLYATISGSLGVLLPFTSREDHDFFQHLEMHMRSE 180
            +V FHVG+ VT LQ+A++IPGGSE+++Y T+ GS+G L  FTSR+D DFF HLEMHMR E
Sbjct: 1090 IVQFHVGDVVTCLQKASMIPGGSESIMYGTVMGSIGALHAFTSRDDVDFFSHLEMHMRQE 1149

Query: 181  NSPLCGRDHLSFRSYYYPVKNVIDGDLCEQFNSLDPGKQKAIAGDLERTPAEVSKKLEDI 360
              PLCGRDH+++RS Y+PVK+VIDGDLCEQF +L    Q+ IA +L+RTPAE+ KKLED 
Sbjct: 1150 YPPLCGRDHMAYRSAYFPVKDVIDGDLCEQFPTLPMDLQRKIADELDRTPAEILKKLEDA 1209

Query: 361  RTR 369
            R +
Sbjct: 1210 RNK 1212


>At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A
            subunit region (PF03178); contains weak WD-40 repeat
            (PF00400); similar to Splicing factor 3B subunit 3
            (SF3b130)/spliceosomal protein/Splicing factor 3B subunit
            3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
            EMBL:HSAJ1443_1
          Length = 1214

 Score =  176 bits (429), Expect = 5e-45
 Identities = 77/123 (62%), Positives = 99/123 (80%)
 Frame = +1

Query: 1    VVNFHVGETVTSLQRATLIPGGSEALLYATISGSLGVLLPFTSREDHDFFQHLEMHMRSE 180
            +V FHVG+ VT LQ+A++IPGGSE+++Y T+ GS+G L  FTSR+D DFF HLEMHMR E
Sbjct: 1090 IVQFHVGDVVTCLQKASMIPGGSESIMYGTVMGSIGALHAFTSRDDVDFFSHLEMHMRQE 1149

Query: 181  NSPLCGRDHLSFRSYYYPVKNVIDGDLCEQFNSLDPGKQKAIAGDLERTPAEVSKKLEDI 360
              PLCGRDH+++RS Y+PVK+VIDGDLCEQF +L    Q+ IA +L+RTPAE+ KKLED 
Sbjct: 1150 YPPLCGRDHMAYRSAYFPVKDVIDGDLCEQFPTLPMDLQRKIADELDRTPAEILKKLEDA 1209

Query: 361  RTR 369
            R +
Sbjct: 1210 RNK 1212


>At1g35240.1 68414.m04370 transcriptional factor B3 family protein /
           auxin-responsive factor AUX/IAA-related contains Pfam
           profiles: PF02309 AUX/IAA family, PF02362: B3 DNA
           binding domain
          Length = 615

 Score = 27.5 bits (58), Expect = 4.5
 Identities = 13/36 (36%), Positives = 17/36 (47%), Gaps = 2/36 (5%)
 Frame = +1

Query: 187 PLCGRDHLSFRSYYYPVKNV--IDGDLCEQFNSLDP 288
           PLC    L    YY+P  N+  +D    E+ N L P
Sbjct: 35  PLCDIPKLGENVYYFPQGNIELVDASTREELNELQP 70


>At5g51120.1 68418.m06339 polyadenylate-binding protein, putative /
           PABP, putative contains similarity to poly(A)-binding
           protein II [Mus musculus] GI:2351846; contains InterPro
           entry IPR000504: RNA-binding region RNP-1 (RNA
           recognition motif) (RRM)
          Length = 227

 Score = 27.1 bits (57), Expect = 6.0
 Identities = 12/27 (44%), Positives = 17/27 (62%)
 Frame = +1

Query: 289 GKQKAIAGDLERTPAEVSKKLEDIRTR 369
           G++ A AGD E  P   S+ LED++ R
Sbjct: 34  GEEGAAAGDEELEPGSSSRDLEDMKKR 60


>At5g35560.1 68418.m04231 DENN (AEX-3) domain-containing protein
           contains Pfam domain PF02141: DENN (AEX-3) domain; this
           cDNA may contain an anomalously spliced intron...will
           require further examination.
          Length = 765

 Score = 26.6 bits (56), Expect = 7.9
 Identities = 9/20 (45%), Positives = 15/20 (75%)
 Frame = -1

Query: 302 AFCFPGSRELNCSHRSPSIT 243
           +FCFPG  E++   R+PS++
Sbjct: 181 SFCFPGGIEVHAVERTPSMS 200


>At2g41710.2 68415.m05155 ovule development protein, putative
           similar to ovule development protein AINTEGUMENTA
           (GI:1209099) [Arabidopsis thaliana];Pfam domain
           (PF00847)
          Length = 428

 Score = 26.6 bits (56), Expect = 7.9
 Identities = 13/44 (29%), Positives = 22/44 (50%)
 Frame = +1

Query: 157 LEMHMRSENSPLCGRDHLSFRSYYYPVKNVIDGDLCEQFNSLDP 288
           +E  + S    L G   LS +   YP+ +++   L   +N+LDP
Sbjct: 347 IEKPVVSHGVALGGAAALSLQKSMYPLTSLLTAPLLTNYNTLDP 390


>At2g41710.1 68415.m05154 ovule development protein, putative
           similar to ovule development protein AINTEGUMENTA
           (GI:1209099) [Arabidopsis thaliana];Pfam domain
           (PF00847)
          Length = 423

 Score = 26.6 bits (56), Expect = 7.9
 Identities = 13/44 (29%), Positives = 22/44 (50%)
 Frame = +1

Query: 157 LEMHMRSENSPLCGRDHLSFRSYYYPVKNVIDGDLCEQFNSLDP 288
           +E  + S    L G   LS +   YP+ +++   L   +N+LDP
Sbjct: 342 IEKPVVSHGVALGGAAALSLQKSMYPLTSLLTAPLLTNYNTLDP 385


>At1g35520.1 68414.m04410 transcriptional factor B3 family protein
           contains Pfam profile: PF02362 B3 DNA binding domain
          Length = 598

 Score = 26.6 bits (56), Expect = 7.9
 Identities = 12/36 (33%), Positives = 18/36 (50%), Gaps = 2/36 (5%)
 Frame = +1

Query: 187 PLCGRDHLSFRSYYYPVKNV--IDGDLCEQFNSLDP 288
           PLC    L  + YY+P  N+  ++    E+ N L P
Sbjct: 35  PLCDIPKLGEKVYYFPQGNIELVEASTREELNELQP 70


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,470,778
Number of Sequences: 28952
Number of extensions: 185919
Number of successful extensions: 412
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 409
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 412
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 752336160
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)