SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc10h21
         (649 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC9.12c |atp12||F1-ATPase chaperone Atp12 |Schizosaccharomyces...    53   3e-08
SPAC4A8.16c |tif33|SPAC823.01c|translation initiation factor eIF...    28   1.3  
SPBC1706.03 |fzo1|SPBC839.01|mitochondrial fusion GTPase protein...    28   1.3  
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce...    27   2.3  
SPBC28F2.08c |||HRD ubiquitin ligase complex subunit |Schizosacc...    27   3.1  
SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb...    26   4.1  
SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch...    26   5.4  
SPAC328.08c |||tubulin specific chaperone cofactor C |Schizosacc...    25   9.4  

>SPAC9.12c |atp12||F1-ATPase chaperone Atp12 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 287

 Score = 53.2 bits (122), Expect = 3e-08
 Identities = 33/108 (30%), Positives = 60/108 (55%), Gaps = 5/108 (4%)
 Frame = +2

Query: 266 PYATRKRFYRGTAVIQNDNKWEVTLDHRRLXTPNGNVLTVGNEP--LARAVAVEWDSQNE 439
           P  + +RF++ TA    + +  + LD R L +P+G ++ V  E   LA  +A+EWD    
Sbjct: 34  PQPSFRRFWKNTATKIQNGEVLIQLDGRNLKSPSGKIVKVPKEMELLAHLIALEWDRLPS 93

Query: 440 T-ISQATMHLTALCNTALD-NPGKLTSHDIVN-YLLEYYPTDTLLFYS 574
           T + Q  + +T+L + A+D +  K    ++++  L+ +  TDT+L YS
Sbjct: 94  TSVRQHNLPITSLVSRAIDISQFKKEEKELLSTQLIRFLDTDTILIYS 141


>SPAC4A8.16c |tif33|SPAC823.01c|translation initiation factor
           eIF3c|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 918

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 14/33 (42%), Positives = 21/33 (63%)
 Frame = +2

Query: 197 IFTLASGISTLLKTRCTLCSIYRPYATRKRFYR 295
           ++   +G+S LL+TR  LC IY   A + RFY+
Sbjct: 562 VYLYNNGVS-LLRTRAMLCHIYHE-ALQNRFYK 592


>SPBC1706.03 |fzo1|SPBC839.01|mitochondrial fusion GTPase
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 758

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 18/54 (33%), Positives = 25/54 (46%)
 Frame = +2

Query: 353 LXTPNGNVLTVGNEPLARAVAVEWDSQNETISQATMHLTALCNTALDNPGKLTS 514
           + T +  VL +    L R    +WD Q ETIS A   L  +C  A    G++ S
Sbjct: 685 IGTESRKVLNIPLNDLRRMFHQQWDKQRETISVAENDL-RICQKARKFFGEIES 737


>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 674

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 13/25 (52%), Positives = 16/25 (64%)
 Frame = +2

Query: 371 NVLTVGNEPLARAVAVEWDSQNETI 445
           NVL V NEP A A+A   D +N+ I
Sbjct: 212 NVLRVVNEPTAAALAYGLDKKNDAI 236


>SPBC28F2.08c |||HRD ubiquitin ligase complex subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 713

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 16/37 (43%), Positives = 23/37 (62%), Gaps = 1/37 (2%)
 Frame = +2

Query: 389 NEPLARAVAVEWDSQNETISQATMHL-TALCNTALDN 496
           N+PLA AVA +    N  I +AT+HL  A+ N  L++
Sbjct: 350 NDPLAFAVAGKVSLANGQIDEATVHLIRAVSNGHLES 386


>SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 741

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 11/33 (33%), Positives = 20/33 (60%)
 Frame = +2

Query: 428 SQNETISQATMHLTALCNTALDNPGKLTSHDIV 526
           S+N+T + +++ +  L N A+D P   T  D+V
Sbjct: 612 SRNQTPTHSSIAVDTLSNVAVDQPAISTPSDVV 644


>SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein
           homolog|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2609

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 20/59 (33%), Positives = 27/59 (45%)
 Frame = +2

Query: 272 ATRKRFYRGTAVIQNDNKWEVTLDHRRLXTPNGNVLTVGNEPLARAVAVEWDSQNETIS 448
           A R+ FY   A IQ+D+   +        T +       NE L    A E DSQ+ET+S
Sbjct: 111 AIRRMFYSFCA-IQDDSNISIYAIGTSSKTDSSIQAASPNEDLKSEGAQEIDSQSETVS 168


>SPAC328.08c |||tubulin specific chaperone cofactor C
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 259

 Score = 25.0 bits (52), Expect = 9.4
 Identities = 9/20 (45%), Positives = 12/20 (60%)
 Frame = -2

Query: 519 SCDVNFPGLSRAVLHNAVKC 460
           SC+++    S   LHNA KC
Sbjct: 153 SCNISISNCSSVNLHNATKC 172


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,792,450
Number of Sequences: 5004
Number of extensions: 59091
Number of successful extensions: 132
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -