BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmnc10h12 (725 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 29 0.89 SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces ... 28 1.6 SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc... 27 2.1 SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1 |Schiz... 27 2.7 SPCC1235.03 |||SMR and CUE domain protein|Schizosaccharomyces po... 27 3.6 SPAC3F10.09 |||1-|Schizosaccharomyces pombe|chr 1|||Manual 26 6.3 SPCC1682.06 |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 26 6.3 SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces pomb... 25 8.3 SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe... 25 8.3 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 28.7 bits (61), Expect = 0.89 Identities = 29/133 (21%), Positives = 63/133 (47%), Gaps = 3/133 (2%) Frame = -3 Query: 399 TSLEPNRFRLRPSDRFCGRRNANSLLPTSHTARLWHSCGLLGLAITSCAMSAMRLASCAV 220 +SL + +PS +S PTS ++ S ++ + + + S+ L S ++ Sbjct: 176 SSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSSSYLSSSSVVSSSSSPSSSSSSTLTSSSL 235 Query: 219 SRRAFSQSCRAVSASRRATIISLANKMSDRLASAKSALATV---NFCVRSSILAITSFVS 49 S + S + S+S +T SL++ S AS+ S+ +++ + SS + +S +S Sbjct: 236 S----TSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSSSSSSPTSTSSTIS 291 Query: 48 ISTAGAYLPVHST 10 S++ + P ++ Sbjct: 292 SSSSSSSSPTSTS 304 >SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1147 Score = 27.9 bits (59), Expect = 1.6 Identities = 20/60 (33%), Positives = 28/60 (46%) Frame = +2 Query: 59 DVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDCENARRETAQLAN 238 D +KID LT+KL VAN AE N +L A E + + + + +E Q N Sbjct: 618 DSASKIDSLTEKLKVAN---AEKNAALCEAALEKVPLAKHNKLSDGTFSTPDKENVQSTN 674 >SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosaccharomyces pombe|chr 2|||Manual Length = 441 Score = 27.5 bits (58), Expect = 2.1 Identities = 17/46 (36%), Positives = 24/46 (52%), Gaps = 4/46 (8%) Frame = +2 Query: 113 DLAEANRSLILF----ANEMIVARRDAETARQDCENARRETAQLAN 238 DL +ANR L ANE + +RD R+D + +R+ QL N Sbjct: 318 DLLDANRILSTACKKAANEKLAVQRDLSKLREDRLSVQRKKIQLRN 363 >SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 440 Score = 27.1 bits (57), Expect = 2.7 Identities = 16/53 (30%), Positives = 31/53 (58%) Frame = +2 Query: 335 AFLRPQKRSLGRSLKRLGSNDVIFSSDYVPNSMNVLNKVKEAIPRNKFKAKHN 493 ++ R + RS+GR ++ + + +PN+ N L+ V+ IP+N+ K KH+ Sbjct: 285 SYFREKVRSVGRLVRA-----ICILNHPIPNTDN-LDSVQIIIPQNQVKRKHD 331 >SPCC1235.03 |||SMR and CUE domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 399 Score = 26.6 bits (56), Expect = 3.6 Identities = 14/75 (18%), Positives = 36/75 (48%) Frame = +2 Query: 56 NDVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDCENARRETAQLA 235 ND++ D+L + ++ E ++ +N +R+ + ++ N+R+ ++ Sbjct: 19 NDLVRSSDELLNHRAIQESEQIETAADALITSNIGHRSRQRKKKTKK-ATNSRKPLSKFQ 77 Query: 236 NRMADIAQDVIAKPS 280 + ++ +D I KPS Sbjct: 78 SNTEEVNEDPILKPS 92 >SPAC3F10.09 |||1-|Schizosaccharomyces pombe|chr 1|||Manual Length = 264 Score = 25.8 bits (54), Expect = 6.3 Identities = 9/15 (60%), Positives = 11/15 (73%) Frame = +3 Query: 342 CARKNDRWAAA*NDW 386 C RK++RW AA N W Sbjct: 140 CRRKDNRWFAAINKW 154 >SPCC1682.06 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 238 Score = 25.8 bits (54), Expect = 6.3 Identities = 18/62 (29%), Positives = 27/62 (43%) Frame = +2 Query: 29 YAPAVEMDTNDVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDCEN 208 YAP+V + I L + + A LA SL+LF + V +D ET + + Sbjct: 146 YAPSVAKANEGLAKAIAGLAKYVAKAIQGLAHIILSLLLFILGLEVIEQDEETGDVEMSS 205 Query: 209 AR 214 R Sbjct: 206 MR 207 >SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 782 Score = 25.4 bits (53), Expect = 8.3 Identities = 23/95 (24%), Positives = 43/95 (45%), Gaps = 11/95 (11%) Frame = +2 Query: 344 RPQKRSLGRSLKRLGSNDVIFSSDYVPNS-----MNVLNKVKEAIPRNKFKAKHNR---- 496 +P ++L + ++ + S++ +P S +N + ++E +P K K N Sbjct: 166 KPFVQALKLTFEKTLGQPAVTSTEKIPVSETTRNVNSQHLMRERVPGEKILPKSNERNIL 225 Query: 497 ITLLEDYTRE--ELMNVIGSTMTDRQIARMNSLRN 595 IT Y L N++GST++ AR + RN Sbjct: 226 ITSALPYVNNVPHLGNIVGSTLSADVFARYHRARN 260 >SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe|chr 1|||Manual Length = 716 Score = 25.4 bits (53), Expect = 8.3 Identities = 9/21 (42%), Positives = 17/21 (80%) Frame = +2 Query: 143 LFANEMIVARRDAETARQDCE 205 +FANE+++ + DA++ +QD E Sbjct: 556 IFANEILIEQADAQSFKQDEE 576 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,837,730 Number of Sequences: 5004 Number of extensions: 54021 Number of successful extensions: 151 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 146 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 151 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 341222980 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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