SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc10h12
         (725 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    29   0.89 
SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces ...    28   1.6  
SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc...    27   2.1  
SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1 |Schiz...    27   2.7  
SPCC1235.03 |||SMR and CUE domain protein|Schizosaccharomyces po...    27   3.6  
SPAC3F10.09 |||1-|Schizosaccharomyces pombe|chr 1|||Manual             26   6.3  
SPCC1682.06 |||sequence orphan|Schizosaccharomyces pombe|chr 3||...    26   6.3  
SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces pomb...    25   8.3  
SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe...    25   8.3  

>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 28.7 bits (61), Expect = 0.89
 Identities = 29/133 (21%), Positives = 63/133 (47%), Gaps = 3/133 (2%)
 Frame = -3

Query: 399 TSLEPNRFRLRPSDRFCGRRNANSLLPTSHTARLWHSCGLLGLAITSCAMSAMRLASCAV 220
           +SL  +    +PS         +S  PTS ++    S  ++  + +  + S+  L S ++
Sbjct: 176 SSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSSSYLSSSSVVSSSSSPSSSSSSTLTSSSL 235

Query: 219 SRRAFSQSCRAVSASRRATIISLANKMSDRLASAKSALATV---NFCVRSSILAITSFVS 49
           S    + S  + S+S  +T  SL++  S   AS+ S+ +++   +    SS  + +S +S
Sbjct: 236 S----TSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSSSSSSPTSTSSTIS 291

Query: 48  ISTAGAYLPVHST 10
            S++ +  P  ++
Sbjct: 292 SSSSSSSSPTSTS 304


>SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1147

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 20/60 (33%), Positives = 28/60 (46%)
 Frame = +2

Query: 59  DVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDCENARRETAQLAN 238
           D  +KID LT+KL VAN   AE N +L   A E +   +  + +        +E  Q  N
Sbjct: 618 DSASKIDSLTEKLKVAN---AEKNAALCEAALEKVPLAKHNKLSDGTFSTPDKENVQSTN 674


>SPBC21.01 |mis17|SPBC776.19|kinetochore protein
           Mis17|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 441

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 17/46 (36%), Positives = 24/46 (52%), Gaps = 4/46 (8%)
 Frame = +2

Query: 113 DLAEANRSLILF----ANEMIVARRDAETARQDCENARRETAQLAN 238
           DL +ANR L       ANE +  +RD    R+D  + +R+  QL N
Sbjct: 318 DLLDANRILSTACKKAANEKLAVQRDLSKLREDRLSVQRKKIQLRN 363


>SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 440

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 16/53 (30%), Positives = 31/53 (58%)
 Frame = +2

Query: 335 AFLRPQKRSLGRSLKRLGSNDVIFSSDYVPNSMNVLNKVKEAIPRNKFKAKHN 493
           ++ R + RS+GR ++      +   +  +PN+ N L+ V+  IP+N+ K KH+
Sbjct: 285 SYFREKVRSVGRLVRA-----ICILNHPIPNTDN-LDSVQIIIPQNQVKRKHD 331


>SPCC1235.03 |||SMR and CUE domain protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 399

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 14/75 (18%), Positives = 36/75 (48%)
 Frame = +2

Query: 56  NDVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDCENARRETAQLA 235
           ND++   D+L     +  ++  E     ++ +N    +R+  +  ++   N+R+  ++  
Sbjct: 19  NDLVRSSDELLNHRAIQESEQIETAADALITSNIGHRSRQRKKKTKK-ATNSRKPLSKFQ 77

Query: 236 NRMADIAQDVIAKPS 280
           +   ++ +D I KPS
Sbjct: 78  SNTEEVNEDPILKPS 92


>SPAC3F10.09 |||1-|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 264

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 9/15 (60%), Positives = 11/15 (73%)
 Frame = +3

Query: 342 CARKNDRWAAA*NDW 386
           C RK++RW AA N W
Sbjct: 140 CRRKDNRWFAAINKW 154


>SPCC1682.06 |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 238

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 18/62 (29%), Positives = 27/62 (43%)
 Frame = +2

Query: 29  YAPAVEMDTNDVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDCEN 208
           YAP+V      +   I  L + +  A   LA    SL+LF   + V  +D ET   +  +
Sbjct: 146 YAPSVAKANEGLAKAIAGLAKYVAKAIQGLAHIILSLLLFILGLEVIEQDEETGDVEMSS 205

Query: 209 AR 214
            R
Sbjct: 206 MR 207


>SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 782

 Score = 25.4 bits (53), Expect = 8.3
 Identities = 23/95 (24%), Positives = 43/95 (45%), Gaps = 11/95 (11%)
 Frame = +2

Query: 344 RPQKRSLGRSLKRLGSNDVIFSSDYVPNS-----MNVLNKVKEAIPRNKFKAKHNR---- 496
           +P  ++L  + ++      + S++ +P S     +N  + ++E +P  K   K N     
Sbjct: 166 KPFVQALKLTFEKTLGQPAVTSTEKIPVSETTRNVNSQHLMRERVPGEKILPKSNERNIL 225

Query: 497 ITLLEDYTRE--ELMNVIGSTMTDRQIARMNSLRN 595
           IT    Y      L N++GST++    AR +  RN
Sbjct: 226 ITSALPYVNNVPHLGNIVGSTLSADVFARYHRARN 260


>SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 716

 Score = 25.4 bits (53), Expect = 8.3
 Identities = 9/21 (42%), Positives = 17/21 (80%)
 Frame = +2

Query: 143 LFANEMIVARRDAETARQDCE 205
           +FANE+++ + DA++ +QD E
Sbjct: 556 IFANEILIEQADAQSFKQDEE 576


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,837,730
Number of Sequences: 5004
Number of extensions: 54021
Number of successful extensions: 151
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -