BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmnc10g05 (694 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Sc... 87 3e-18 SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Sc... 82 6e-17 SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 29 0.48 SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce... 26 4.5 SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Sp... 26 5.9 SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|ch... 26 5.9 SPAC23A1.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 7.9 >SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Schizosaccharomyces pombe|chr 1|||Manual Length = 117 Score = 86.6 bits (205), Expect = 3e-18 Identities = 41/59 (69%), Positives = 48/59 (81%) Frame = +2 Query: 65 APKMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLR 241 AP +KK KK+ ++INS+LAL MKSGKY LGYK TLKTLR GKAKL++IA NAPPLR Sbjct: 8 APVAAVSKKGKKSGDTINSKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAANAPPLR 66 Score = 72.5 bits (170), Expect = 5e-14 Identities = 30/43 (69%), Positives = 37/43 (86%) Frame = +1 Query: 565 RKSEIEYYALLAKTGVHHYSGNNIELGTACGKYYRVCTLAITD 693 RKSE+EYYA+L++ VHHYSGNNI+LGTACGK +RV LA+ D Sbjct: 66 RKSELEYYAMLSRCSVHHYSGNNIDLGTACGKLFRVGVLAVID 108 >SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Schizosaccharomyces pombe|chr 1|||Manual Length = 109 Score = 82.2 bits (194), Expect = 6e-17 Identities = 38/55 (69%), Positives = 45/55 (81%) Frame = +2 Query: 77 VAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLR 241 V KK KK+ ++IN++LAL MKSGKY LGYK TLKTLR GKAKL++IA N PPLR Sbjct: 4 VVTKKSKKSGDTINAKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAGNCPPLR 58 Score = 73.3 bits (172), Expect = 3e-14 Identities = 31/43 (72%), Positives = 37/43 (86%) Frame = +1 Query: 565 RKSEIEYYALLAKTGVHHYSGNNIELGTACGKYYRVCTLAITD 693 RKSE+EYYA+L+K VHHY+G NI+LGTACGK +RV LAITD Sbjct: 58 RKSELEYYAMLSKANVHHYAGTNIDLGTACGKLFRVGVLAITD 100 >SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccharomyces pombe|chr 1|||Manual Length = 433 Score = 29.5 bits (63), Expect = 0.48 Identities = 13/42 (30%), Positives = 24/42 (57%) Frame = +2 Query: 89 KQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVI 214 ++KK I+ ++L SGKYC G T+ L++G + ++ Sbjct: 279 QEKKLIQRFFDEISL--DSGKYCFGVVDTMNALQEGAVETLL 318 >SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 154 Score = 26.2 bits (55), Expect = 4.5 Identities = 17/58 (29%), Positives = 32/58 (55%) Frame = +2 Query: 65 APKMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPL 238 APK + KK KT++ + + ++ G K+ +K +R+G+ LVI+A + P+ Sbjct: 33 APKKLN-KKMMKTVKKASKQKHILR-------GVKEVVKAVRKGEKGLVILAGDISPM 82 >SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Spt6|Schizosaccharomyces pombe|chr 1|||Manual Length = 1365 Score = 25.8 bits (54), Expect = 5.9 Identities = 23/75 (30%), Positives = 33/75 (44%) Frame = +1 Query: 406 FWAYVSFKQIIFNLPLMINYKFD*IAFVS*YCFAGSQCGEPME*SLNSILHFGRKSEIEY 585 FW+ S KQ I L + D V +C A S + ++ LN +HF +I Sbjct: 373 FWSLHSKKQDILKLYSDLGINDD---LVVPFCEAASSL-DAID-DLNDYIHFTYSEQIRD 427 Query: 586 YALLAKTGVHHYSGN 630 ALL TG+ G+ Sbjct: 428 RALLMGTGLRRPQGS 442 >SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|chr 1|||Manual Length = 198 Score = 25.8 bits (54), Expect = 5.9 Identities = 15/59 (25%), Positives = 31/59 (52%), Gaps = 2/59 (3%) Frame = +3 Query: 183 LFDKAKQSWLSSLKMLLR*GEATEK*SFPILV--KGMHMESY*QRSMDSLCGLVKQFAK 353 +FD +++S L+S+K R K + PIL+ K H ++ + + + +++AK Sbjct: 89 MFDLSRKSTLNSIKEWYRQARGFNKTAVPILIGTKYDHFMTFPREDQEEITKQARRYAK 147 >SPAC23A1.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 101 Score = 25.4 bits (53), Expect = 7.9 Identities = 11/30 (36%), Positives = 18/30 (60%) Frame = +3 Query: 477 NCIRFLILFCWITMWGTYGVVFKLDTSLWQ 566 N + F+I+F +T W ++ KL SLW+ Sbjct: 50 NTVVFIIVF--LTGWAAKSIIVKLLPSLWR 77 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,906,441 Number of Sequences: 5004 Number of extensions: 57978 Number of successful extensions: 114 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 112 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 114 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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