Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc10e16
(704 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g45790.1 68418.m05631 expressed protein ; expression support... 36 0.035
At5g45310.1 68418.m05562 expressed protein 33 0.24
At5g55390.1 68418.m06901 hydroxyproline-rich glycoprotein family... 31 0.56
At5g07380.1 68418.m00845 hypothetical protein 31 0.98
At2g27150.1 68415.m03263 aldehyde oxidase 3 (AAO3) identical to ... 29 2.3
At1g09790.1 68414.m01098 phytochelatin synthetase-related contai... 29 2.3
At1g07350.1 68414.m00783 transformer serine/arginine-rich ribonu... 29 3.0
At5g41940.1 68418.m05106 RabGAP/TBC domain-containing protein si... 29 4.0
At4g24530.1 68417.m03516 expressed protein contains Pfam PF03138... 29 4.0
At4g21130.1 68417.m03055 transducin family protein / WD-40 repea... 28 5.2
At1g21750.2 68414.m02723 protein disulfide isomerase, putative s... 28 5.2
At1g21750.1 68414.m02722 protein disulfide isomerase, putative s... 28 5.2
At3g49350.1 68416.m05395 RabGAP/TBC domain-containing protein si... 28 6.9
At2g47220.1 68415.m05897 3' exoribonuclease family domain 1 prot... 28 6.9
At2g22630.1 68415.m02682 MADS-box protein (AGL17) nearly identic... 28 6.9
At5g54210.1 68418.m06753 NLI interacting factor (NIF) family pro... 27 9.2
At4g09570.1 68417.m01575 calcium-dependent protein kinase, putat... 27 9.2
At4g02700.1 68417.m00365 sulfate transporter identical to sulfat... 27 9.2
At3g14050.1 68416.m01773 RelA/SpoT protein, putative (RSH2) near... 27 9.2
>At5g45790.1 68418.m05631 expressed protein ; expression supported
by MPSS
Length = 423
Score = 35.5 bits (78), Expect = 0.035
Identities = 27/103 (26%), Positives = 48/103 (46%)
Frame = +2
Query: 26 CKMCVIFPVEIDVSQTVIRDCHVDEQTRELVYINKIMNTQLTKPVLMMFNISGPIRSVTR 205
CK+ F V ID + V+R+C VD R + K++ L+K ++ + IR
Sbjct: 129 CKITEKFKVLIDEEENVVRECEVDAVKR----VKKLL--LLSKHGVLRVHALRLIRKELG 182
Query: 206 KNNDLRDRIKSKVDEQFDQLEREYSDKIDGFHDNIQYFKDEHY 334
D RD I +K +F ++ E + +D +++ K E +
Sbjct: 183 LPEDFRDSILAKYSSEFRLVDLETLELVDRDDESLCVAKVEEW 225
>At5g45310.1 68418.m05562 expressed protein
Length = 352
Score = 32.7 bits (71), Expect = 0.24
Identities = 21/74 (28%), Positives = 40/74 (54%), Gaps = 5/74 (6%)
Frame = +2
Query: 215 DLRDRIKSK--VDEQFDQLEREYSD---KIDGFHDNIQYFKDEHYSVSCQNGSVLKSKFA 379
+LR+ IK +++ D+LE E+ + KI+ +Q K+E+ + NG +SK
Sbjct: 113 NLREAIKEYRIMEQDLDELEDEHDEAISKIEKLEAELQELKEENLQLMEVNGKDYRSKKG 172
Query: 380 KILKSHDYTDKKSI 421
K+ S + ++ +SI
Sbjct: 173 KVKPSEEPSEIRSI 186
>At5g55390.1 68418.m06901 hydroxyproline-rich glycoprotein family
protein
Length = 1332
Score = 31.5 bits (68), Expect = 0.56
Identities = 25/103 (24%), Positives = 40/103 (38%), Gaps = 6/103 (5%)
Frame = +2
Query: 191 RSVTRKNNDLRDRIKSKVDEQFDQLEREYSDKIDGFHDNIQYFKDEHYSVSCQNGSVLKS 370
R + ++N D I D E E D+ D F +++ D + C GS L+S
Sbjct: 185 RRLPDEDNAKDDFIVGDEDTYVASDEDELDDEDDDFFESVCAICDNGGEILCCEGSCLRS 244
Query: 371 KFAKILKSHDYT------DKKSIETYEKYCLPQLVDKHNDCYV 481
A D +K +E +KY P K + C++
Sbjct: 245 FHATKKDGEDSLCDSLGFNKMQVEAIQKYFCPNCEHKIHQCFI 287
>At5g07380.1 68418.m00845 hypothetical protein
Length = 595
Score = 30.7 bits (66), Expect = 0.98
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +2
Query: 479 VAVCVLKPGFENGSNQVLSFEYNPIGNKVIVPFAHEINDTGL 604
+ +C + +G++Q L F Y P GN + F H IN L
Sbjct: 45 IGLCYILKDRISGNDQQLKFAYTPTGNFCLRDFHHAINSLPL 86
>At2g27150.1 68415.m03263 aldehyde oxidase 3 (AAO3) identical to
GP:3172044:gnl:PID:d1029570:AB010080
Length = 1332
Score = 29.5 bits (63), Expect = 2.3
Identities = 20/51 (39%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = +2
Query: 419 IETYEKYCLPQLVDKHNDCYVAVCVLKPGFENGSNQVL-SFEYNPIGNKVI 568
IE+ + C +KHN+ +V P F + S QVL S E+ PIG VI
Sbjct: 521 IESGHRICSLDSGNKHNNSHVDTVKSLP-FLSSSQQVLESNEFKPIGEAVI 570
>At1g09790.1 68414.m01098 phytochelatin synthetase-related contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 454
Score = 29.5 bits (63), Expect = 2.3
Identities = 20/55 (36%), Positives = 30/55 (54%)
Frame = +2
Query: 491 VLKPGFENGSNQVLSFEYNPIGNKVIVPFAHEINDTGLYEYDVLAYVDSVEFDGK 655
VL P ++ QV SF Y K + P+ + INDTG++ + V Y D + +GK
Sbjct: 328 VLHPNLKS-VQQVFSFNY-----KSLTPYQNSINDTGMF-WGVQFYNDVLLQEGK 375
>At1g07350.1 68414.m00783 transformer serine/arginine-rich
ribonucleoprotein, putative similar to GB:Y09506 from
[Nicotiana tabacum] (Plant Mol. Biol. 35 (3), 261-269
(1997))
Length = 382
Score = 29.1 bits (62), Expect = 3.0
Identities = 12/23 (52%), Positives = 17/23 (73%)
Frame = +1
Query: 430 RKILFAPIGRQTQRLLRGGMRVE 498
R+I F P+GRQ QR+L +R+E
Sbjct: 4 REIHFTPVGRQVQRVLEYPLRLE 26
>At5g41940.1 68418.m05106 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 549
Score = 28.7 bits (61), Expect = 4.0
Identities = 17/49 (34%), Positives = 28/49 (57%), Gaps = 1/49 (2%)
Frame = -2
Query: 619 DVVLVKSRVVNFVSKRHNNFVTNRVVLKR*-HLVAAVLKSRLQHAYRHV 476
D VL V F+SK +NF + V ++R +V+ ++K + H YRH+
Sbjct: 380 DDVLAFWCFVGFMSKARHNFRLDEVGIRRQLSMVSKIIKFKDIHLYRHL 428
>At4g24530.1 68417.m03516 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'PsRT17-1 like protein' based on similarity to PsRT17-1
(GP:1778376) [Pisum sativum] which was based upon
similarity to axi 1 protein (GB:X80301) (GI:559920) from
[Nicotiana tabacum], which, due to scienitific fraud was
retracted. Retraction in: Schell J. EMBO J 1999 May
17;18(10):2908. PMID:10400497.
Length = 519
Score = 28.7 bits (61), Expect = 4.0
Identities = 18/43 (41%), Positives = 20/43 (46%)
Frame = -1
Query: 368 FSTRCHFGNLPNSVHL*NTGCYREIHQFYRCIRVLVDQIVHRL 240
FS R F NLP S+ E F IR L D +VHRL
Sbjct: 226 FSHRLAFDNLPESIQRLRCKVNFEALNFVPHIRELGDALVHRL 268
>At4g21130.1 68417.m03055 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400); some
similarity to a group of proteins with homology to
mammalian apoptosis regulators identified in zebrafish
(PUBMED:10917738)Apaf-1(gi:7677507)
Length = 537
Score = 28.3 bits (60), Expect = 5.2
Identities = 15/33 (45%), Positives = 19/33 (57%), Gaps = 3/33 (9%)
Frame = +2
Query: 83 DCHV---DEQTRELVYINKIMNTQLTKPVLMMF 172
DCHV D +TRE V +NK+ N Q+ V F
Sbjct: 227 DCHVHLWDIRTREHVQVNKLKNNQVKDSVHCAF 259
>At1g21750.2 68414.m02723 protein disulfide isomerase, putative
similar to SP|P29828 Protein disulfide isomerase
precursor (PDI) (EC 5.3.4.1) {Medicago sativa}; isoform
contains non-consensus GA donor splice site at intron 9
Length = 487
Score = 28.3 bits (60), Expect = 5.2
Identities = 10/24 (41%), Positives = 19/24 (79%)
Frame = +2
Query: 626 YVDSVEFDGKQFEEFVQKXNIAVV 697
+VDS +FDG+ E+FV++ +I ++
Sbjct: 223 FVDSKDFDGEALEKFVKESSIPLI 246
>At1g21750.1 68414.m02722 protein disulfide isomerase, putative
similar to SP|P29828 Protein disulfide isomerase
precursor (PDI) (EC 5.3.4.1) {Medicago sativa}; isoform
contains non-consensus GA donor splice site at intron 9
Length = 501
Score = 28.3 bits (60), Expect = 5.2
Identities = 10/24 (41%), Positives = 19/24 (79%)
Frame = +2
Query: 626 YVDSVEFDGKQFEEFVQKXNIAVV 697
+VDS +FDG+ E+FV++ +I ++
Sbjct: 223 FVDSKDFDGEALEKFVKESSIPLI 246
>At3g49350.1 68416.m05395 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 554
Score = 27.9 bits (59), Expect = 6.9
Identities = 13/40 (32%), Positives = 25/40 (62%), Gaps = 1/40 (2%)
Frame = -2
Query: 592 VNFVSKRHNNFVTNRVVLKR*-HLVAAVLKSRLQHAYRHV 476
V F+ K +NF + V ++R ++V+ ++KS+ YRH+
Sbjct: 389 VGFMKKARHNFRLDEVGIRRQLNIVSKIIKSKDSQLYRHL 428
>At2g47220.1 68415.m05897 3' exoribonuclease family domain 1
protein-related similar to polynucleotide phosphorylase
[Pisum sativum] GI:2286200, polyribonucleotide
phophorylase [Spinacia oleracea] GI:1924972; contains
Pfam profiles PF05266: Protein of unknown function
(DUF724), weak hit to PF01138: 3' exoribonuclease
family, domain 1
Length = 469
Score = 27.9 bits (59), Expect = 6.9
Identities = 22/91 (24%), Positives = 41/91 (45%)
Frame = +2
Query: 170 FNISGPIRSVTRKNNDLRDRIKSKVDEQFDQLEREYSDKIDGFHDNIQYFKDEHYSVSCQ 349
F ++ P+ + K L+DR + K+ E+ ++E D+ H Q F + +
Sbjct: 369 FTVTAPLSRIA-KLLALKDR-QLKILEELKVFDKEMKDESSKKHKAEQEFGEMERKIL-- 424
Query: 350 NGSVLKSKFAKILKSHDYTDKKSIETYEKYC 442
+K+K ++ K +K+ TYEK C
Sbjct: 425 ---EVKNKVLELQKQEAALEKQKDATYEKIC 452
>At2g22630.1 68415.m02682 MADS-box protein (AGL17) nearly identical
to MADS-box protein AGL17 [Arabidopsis thaliana]
GI:862648
Length = 227
Score = 27.9 bits (59), Expect = 6.9
Identities = 18/61 (29%), Positives = 27/61 (44%)
Frame = -2
Query: 187 RTRNVKHHENGFCQLRVHNLVNVHQLSGLFVHMTISNNRLGHVDFYRKNYTHFAMVKLKC 8
R RN+ HHEN +V + + + T + N LGH + Y A V+L+
Sbjct: 154 RKRNLVHHENLELSRKVQRIHQENVELYKKAYGTSNTNGLGHHELVDAVYESHAQVRLQL 213
Query: 7 S 5
S
Sbjct: 214 S 214
>At5g54210.1 68418.m06753 NLI interacting factor (NIF) family
protein contains Pfam profile PF03031: NLI interacting
factor
Length = 306
Score = 27.5 bits (58), Expect = 9.2
Identities = 16/33 (48%), Positives = 20/33 (60%)
Frame = +1
Query: 133 YEHAIDKTRSHDV*HFGSYTKRYAQEQRFARQN 231
Y + DKTR HDV YTK YA+E+R +N
Sbjct: 231 YNYFSDKTR-HDV----KYTKSYAEEKRDESRN 258
>At4g09570.1 68417.m01575 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Arabidopsis thaliana] gi|604881|dbj|BAA04830;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 501
Score = 27.5 bits (58), Expect = 9.2
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 383 ILKSHDYTDKKSIETYEKYCLPQLVDKH 466
I K D + KK I +E C P +VD+H
Sbjct: 260 IYKMLDRSPKKRISAHEALCHPWIVDEH 287
>At4g02700.1 68417.m00365 sulfate transporter identical to sulfate
transporter [Arabidopsis thaliana] GI:2130944
Length = 646
Score = 27.5 bits (58), Expect = 9.2
Identities = 15/40 (37%), Positives = 24/40 (60%)
Frame = +2
Query: 146 LTKPVLMMFNISGPIRSVTRKNNDLRDRIKSKVDEQFDQL 265
+T+ L++ +I GPI + LRDRI +DE+ D+L
Sbjct: 509 ITRSSLLILHIDGPIYFAN--STYLRDRIGRWIDEEEDKL 546
>At3g14050.1 68416.m01773 RelA/SpoT protein, putative (RSH2) nearly
identical to RelA/SpoT homolog RSH2 [Arabidopsis
thaliana] GI:7141306; contains Pfam profiles PF01966: HD
domain, PF04607: Region found in RelA / SpoT proteins
Length = 709
Score = 27.5 bits (58), Expect = 9.2
Identities = 18/55 (32%), Positives = 27/55 (49%)
Frame = +2
Query: 332 YSVSCQNGSVLKSKFAKILKSHDYTDKKSIETYEKYCLPQLVDKHNDCYVAVCVL 496
Y V C L S ++K+LK D E ++ + L +VD DCY A+ V+
Sbjct: 422 YHVLCGRHKSLYSIYSKMLKKKLTVD----EIHDIHGLRLIVDNEGDCYKALGVV 472
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,302,372
Number of Sequences: 28952
Number of extensions: 290028
Number of successful extensions: 893
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 877
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 893
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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