BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc10c20
(692 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC14C8.07c |cdc18||MCM loader|Schizosaccharomyces pombe|chr 2|... 28 1.5
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi... 28 1.5
SPAC22H10.05c |||mRNA cleavage and polyadenylation specificity f... 27 2.6
SPAC1805.03c |trm13||tRNA 2'-O-methyltransferase Trm13 |Schizosa... 27 2.6
SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po... 26 4.5
SPBC3B9.16c |nup120||nucleoporin Nup120|Schizosaccharomyces pomb... 26 5.9
>SPBC14C8.07c |cdc18||MCM loader|Schizosaccharomyces pombe|chr
2|||Manual
Length = 577
Score = 27.9 bits (59), Expect = 1.5
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = -1
Query: 410 QSPKLRKRTTNVLLPPALSAVCEEFPWRSQ 321
Q+PK KRT ++ P +L+ C P+ ++
Sbjct: 100 QTPKTPKRTIQIVTPKSLNRTCNPVPFATR 129
>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 865
Score = 27.9 bits (59), Expect = 1.5
Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 2/47 (4%)
Frame = +1
Query: 505 GRRTTASRIRRIPVVWRSNANLR*VLNPEDFD*--NDSVLTKTVQEP 639
G +TASRI +I ++ N N +F NDS L+KT+ EP
Sbjct: 571 GSSSTASRIDKIIYSYKRNKNTEVQQRSVEFHLILNDSKLSKTILEP 617
>SPAC22H10.05c |||mRNA cleavage and polyadenylation specificity
factor complex subunit |Schizosaccharomyces pombe|chr
1|||Manual
Length = 456
Score = 27.1 bits (57), Expect = 2.6
Identities = 19/45 (42%), Positives = 21/45 (46%)
Frame = +2
Query: 389 VCGASETGAHSSTR*EAIEERNTPDGYQVHRTSLRGPGMAEEPQR 523
V ASE+G SS EE NT Q TSLR G E +R
Sbjct: 339 VLEASESGPKSSVLPLGFEEENTQSEKQDGNTSLRLHGKGEFLER 383
>SPAC1805.03c |trm13||tRNA 2'-O-methyltransferase Trm13
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 407
Score = 27.1 bits (57), Expect = 2.6
Identities = 13/36 (36%), Positives = 16/36 (44%), Gaps = 4/36 (11%)
Frame = -3
Query: 282 SVLFSAVCCHHRCRGDT----GRHSASHCQAPRPNK 187
+ L A+CCHH CR T R SH P +
Sbjct: 285 NALVIALCCHHHCRWRTLSTFAREQLSHWGISNPQE 320
>SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 811
Score = 26.2 bits (55), Expect = 4.5
Identities = 32/123 (26%), Positives = 54/123 (43%), Gaps = 1/123 (0%)
Frame = +3
Query: 261 RQPRT-IQNISEELSNLNPCLLRPPRKLFTNCRERWRQQNVSGAFAELRRLVPTHPPDKK 437
++P+T IQ +S + + +P L+ R LF N ++ N + A E +P +
Sbjct: 680 KKPKTFIQWVSRDKESNSPVLIAETR-LFNNL---FKCDNPA-ALKEQELAAQLNPESEV 734
Query: 438 LSKNEILRMAIRYIGLLCEVLEWQKNHSVTNKENSGSLAIKCESPLSPQSRRLRLKRQCF 617
+ KN I+ I L + W K S + K E+P S + + +R+ C
Sbjct: 735 VLKNSIIEPGIYD---LIKSAPWPKTDSSAGVD-------KAENPESVRFQAMRVGYFCL 784
Query: 618 DED 626
DED
Sbjct: 785 DED 787
>SPBC3B9.16c |nup120||nucleoporin Nup120|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1136
Score = 25.8 bits (54), Expect = 5.9
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -2
Query: 559 LIARLPEFSLFVTLWFF 509
L+ LP +SLF TLW F
Sbjct: 602 LVQDLPSYSLFDTLWVF 618
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,761,130
Number of Sequences: 5004
Number of extensions: 55443
Number of successful extensions: 152
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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