BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmnc10c19 (718 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23H4.09 |cdb4||curved DNA-binding protein Cdb4|Schizosacchar... 206 2e-54 SPBC23E6.05 |arx1||ribosomal export complex Arx1 |Schizosaccharo... 97 3e-21 SPBC14C8.03 |fma2||methionine aminopeptidase Fma2 |Schizosacchar... 65 1e-11 SPBC3E7.10 |fma1||methionine aminopeptidase Fma1 |Schizosaccharo... 35 0.013 SPAC922.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 32 0.094 SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 29 0.66 SPAC23H3.08c |bub3||mitotic spindle checkpoint protein Bub3|Schi... 26 4.7 SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharo... 26 4.7 SPAC17C9.09c |tim13||TIM22 inner membrane protein import complex... 26 4.7 SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 6.2 SPBC16A3.03c |lyn1||sequence orphan|Schizosaccharomyces pombe|ch... 26 6.2 SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 26 6.2 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 25 8.2 SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 25 8.2 >SPAC23H4.09 |cdb4||curved DNA-binding protein Cdb4|Schizosaccharomyces pombe|chr 1|||Manual Length = 381 Score = 206 bits (504), Expect = 2e-54 Identities = 104/231 (45%), Positives = 145/231 (62%), Gaps = 2/231 (0%) Frame = +1 Query: 28 EMADEKEVEKTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEET 207 E E V+ +++ V KYK+AG++ V+++V+ C P A +IC GD+L+ E Sbjct: 5 EATSETAVDYSLSNPETVNKYKIAGEVSQNVIKKVVELCQPGAKIYDICVRGDELLNEAI 64 Query: 208 NKVFKKEKDSKKGIAFSTCVSVNNCICHFSPIASEPD--YILKKGDLAKIDLGAHIDGFI 381 KV++ KD+ KGIAF T VS N+ H SP+ S+P+ LK GD+ KI LGAHIDGF Sbjct: 65 KKVYRT-KDAYKGIAFPTAVSPNDMAAHLSPLKSDPEANLALKSGDVVKILLGAHIDGFA 123 Query: 382 AVVAHTVVVGESEVSGRAADVXXXXXXXXXXXXXXXKPGTENYAVTEAIQKISAEYGCKP 561 ++VA T VV E V+G AADV KPG N+ VT+ + KI+ YGCKP Sbjct: 124 SLVATTTVVSEEPVTGPAADVIAAASAALKAAQRTIKPGNTNWQVTDIVDKIATSYGCKP 183 Query: 562 IEGMLSHQLKQFRIDGEKSIILNPSEAQRKEHEKATLEKYEVYAMDVLIST 714 + GMLSHQ ++ IDG+K +ILNPS++QR E + T E+ EVY +D+L+ST Sbjct: 184 VAGMLSHQQEREVIDGKKQVILNPSDSQRSEMDTFTFEEGEVYGVDILVST 234 >SPBC23E6.05 |arx1||ribosomal export complex Arx1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 417 Score = 96.7 bits (230), Expect = 3e-21 Identities = 46/130 (35%), Positives = 78/130 (60%), Gaps = 6/130 (4%) Frame = +1 Query: 37 DEKEVEKTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEETNKV 216 D + + + +KY+ AG +V++ QV ++CVP AS REI +GD L+ E + + Sbjct: 4 DPSDSNSRVVDASQFSKYRDAGALVSKAFHQVASRCVPGASTREISSYGDNLLHEYKSSI 63 Query: 217 FKKEKDSKKGIAFSTCVSVNNCICHFSP------IASEPDYILKKGDLAKIDLGAHIDGF 378 +K ++ +KGIA T + VNNC +++P ++ Y L+ GD+ KI +G H DG+ Sbjct: 64 YKSQR-FEKGIAEPTSICVNNCAYNYAPGPESVIAGNDNSYHLQVGDVTKISMGLHFDGY 122 Query: 379 IAVVAHTVVV 408 A+++HT+VV Sbjct: 123 TALISHTIVV 132 >SPBC14C8.03 |fma2||methionine aminopeptidase Fma2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 64.9 bits (151), Expect = 1e-11 Identities = 48/179 (26%), Positives = 81/179 (45%), Gaps = 5/179 (2%) Frame = +1 Query: 196 LEETNKVFKKEKDSKKGIAFSTCVSVNNCICHFSPIASEPDYILKKGDLAKIDLGAHIDG 375 +E T + +E K GI F T VS+N+C H++P A + ILK+ D+ K+D+G H++G Sbjct: 148 IENTTRALVEEDGLKSGIGFPTGVSLNHCAAHYTPNAGDTT-ILKEKDVMKVDIGVHVNG 206 Query: 376 FIAVVAHTVVVGESEVSGRAADVXXXXXXXXXXXXXXXKPGTENYAVTEAIQKISAE--- 546 I A T+ + + A V + A+ E ++ E Sbjct: 207 RIVDSAFTMSF-DPQYDNLLAAVKAATNKGIEEAGIDARLNEIGEAIQEVMESYEVEING 265 Query: 547 --YGCKPIEGMLSHQLKQFRIDGEKSIILNPSEAQRKEHEKATLEKYEVYAMDVLISTG 717 + K I + H L + I G KS+ + K E+ +E+ E++A++ STG Sbjct: 266 KTHQVKSIRNLCGHNLDPYIIHGGKSVPI------VKGGEEIKMEEGEIFAIETFGSTG 318 >SPBC3E7.10 |fma1||methionine aminopeptidase Fma1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 34.7 bits (76), Expect = 0.013 Identities = 20/53 (37%), Positives = 27/53 (50%) Frame = +1 Query: 256 STCVSVNNCICHFSPIASEPDYILKKGDLAKIDLGAHIDGFIAVVAHTVVVGE 414 S C SVN ICH P P L+ GD+ ID+ + +GF + T VG+ Sbjct: 181 SVCTSVNEIICHGIP-DQRP---LEDGDIVNIDVSLYHNGFHGDLNETYYVGD 229 >SPAC922.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 117 Score = 31.9 bits (69), Expect = 0.094 Identities = 21/71 (29%), Positives = 33/71 (46%), Gaps = 3/71 (4%) Frame = -2 Query: 561 WLTTIFSTYFLNGFSDCIVLSTR---LQETKSRFTSMMSCQQNICSTSRDFTFTHNYCMS 391 W++++ L G S I LST L + K+ + M + C S +T + N C + Sbjct: 5 WVSSLLG---LLGLSTAIPLSTEADALLDRKTIYFGMKAYDDVNCKGSTSYTISFNKCQA 61 Query: 390 YNSYKSINVST 358 Y + SIN T Sbjct: 62 YPNINSINALT 72 >SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a Pol2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 2199 Score = 29.1 bits (62), Expect = 0.66 Identities = 11/30 (36%), Positives = 22/30 (73%) Frame = -1 Query: 340 NLLFSECNPVRSQWAKSDKCSCSQKRMLKM 251 +L+ S+C V+ QW+ ++CSCS + +L++ Sbjct: 2135 DLICSKCKTVK-QWSLKERCSCSGEWVLQL 2163 >SPAC23H3.08c |bub3||mitotic spindle checkpoint protein Bub3|Schizosaccharomyces pombe|chr 1|||Manual Length = 320 Score = 26.2 bits (55), Expect = 4.7 Identities = 9/19 (47%), Positives = 15/19 (78%) Frame = -3 Query: 650 LRCASDGFSIILFSPSIRN 594 L+ + DG S ++FSPS++N Sbjct: 8 LKNSKDGISSVIFSPSVKN 26 >SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1588 Score = 26.2 bits (55), Expect = 4.7 Identities = 13/32 (40%), Positives = 17/32 (53%) Frame = +2 Query: 143 AFQTHQQEKYVNLAINLYWRRLTRFSKRRKTP 238 AF T +Q Y N NLY+ ++ S R TP Sbjct: 474 AFPTSRQNAYYNDPWNLYFIHFSKLSPLRFTP 505 >SPAC17C9.09c |tim13||TIM22 inner membrane protein import complex subunit Tim13|Schizosaccharomyces pombe|chr 1|||Manual Length = 95 Score = 26.2 bits (55), Expect = 4.7 Identities = 12/40 (30%), Positives = 25/40 (62%), Gaps = 1/40 (2%) Frame = +1 Query: 37 DEKEV-EKTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPD 153 D+K + K I ++L V + AG++++++ E KC+P+ Sbjct: 16 DKKSIFMKQIRQELAVAQ---AGELISKINENCFDKCIPE 52 >SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 357 Score = 25.8 bits (54), Expect = 6.2 Identities = 9/24 (37%), Positives = 14/24 (58%) Frame = -2 Query: 402 YCMSYNSYKSINVSTKIYLG*ISF 331 YC +YKS+ + T +Y+G F Sbjct: 144 YCRKVLTYKSLGIRTAVYVGAFCF 167 >SPBC16A3.03c |lyn1||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 658 Score = 25.8 bits (54), Expect = 6.2 Identities = 17/61 (27%), Positives = 30/61 (49%), Gaps = 3/61 (4%) Frame = -1 Query: 568 PQLAYNHIQHLFSE---WLQ*LHSSQYQASGDEEPLH*HDELPTKHLQHVQRLHFHPQLL 398 P LAY + + FS + +SS + +P ++ L ++ LQ +LH+H +L Sbjct: 375 PSLAYLQVVYSFSMRALLVNRQYSSAFAFFWKIDPYIRNERLVSQMLQAASQLHYHDLIL 434 Query: 397 Y 395 Y Sbjct: 435 Y 435 >SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 681 Score = 25.8 bits (54), Expect = 6.2 Identities = 12/35 (34%), Positives = 19/35 (54%) Frame = -2 Query: 192 KFIAKFTYFSC*CVWNAFCDDLLKDSVHYLSC*LV 88 K + KF+ C +++A C+D L S H C +V Sbjct: 472 KCLNKFSQEDCQFIFDAICEDPLDVSTHRHGCCVV 506 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 25.4 bits (53), Expect = 8.2 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = +1 Query: 154 ASAREICEFGDKLVLEETNKVFKKEKD 234 ++ +E FG+KL E T K+ EKD Sbjct: 865 SNLQEKINFGNKLAKEHTEKISSLEKD 891 >SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 486 Score = 25.4 bits (53), Expect = 8.2 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = +1 Query: 310 EPDYILKKGDLAKIDLGAHIDGFIAVVAHTVV 405 EP+Y+L G+L+ +L ID F T V Sbjct: 382 EPEYLLMYGNLSNEELVERIDAFYMNARRTYV 413 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.317 0.133 0.373 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,845,332 Number of Sequences: 5004 Number of extensions: 57932 Number of successful extensions: 210 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 199 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 204 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 335201398 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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