BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmnc10c06 (728 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP1E11.08 |||ribosome biogenesis protein Nsa2 |Schizosaccharom... 272 3e-74 SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 29 0.68 SPAC23C4.14 |alg1||mannosyltransferase complex subunit Alg1 |Sch... 28 1.2 SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|ch... 28 1.6 SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Sc... 27 3.6 SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c... 27 3.6 SPAC22H12.03 |||mitochondrial hydrolase|Schizosaccharomyces pomb... 26 6.3 SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|ch... 25 8.4 SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 25 8.4 >SPCP1E11.08 |||ribosome biogenesis protein Nsa2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 260 Score = 272 bits (667), Expect = 3e-74 Identities = 130/226 (57%), Positives = 166/226 (73%), Gaps = 1/226 (0%) Frame = +2 Query: 53 MPQNEYIERHQKLYGRRLDYXXXXXXXXXXXXXXXXXXXXXLRGIKAKIFNKERRNEKIQ 232 MPQNEYIE + +GRR D+ RGIKAK++ ++RR EKIQ Sbjct: 1 MPQNEYIEESIRKHGRRFDHEERKRKKAAREAHDASLYAQKTRGIKAKLYQEKRRKEKIQ 60 Query: 233 MKKKIKAHEEKNV-KHNTEKVAEGALPVYLLDRDVQSRAKVLSNMIKQKRKEKAGKWDVP 409 MKK IK HEE+N + ++ +GA+P YLLDR+ +S+AK+LS+ +KQKRKEKA K+ VP Sbjct: 61 MKKTIKQHEERNATQRGSDAQTQGAVPTYLLDREQESQAKMLSSAVKQKRKEKAAKYSVP 120 Query: 410 IPKVRAQADAEVFKVLKSGKSKRKAWKRMVTKVTFVGENFTRKPPKFERFIRPMALRFKK 589 +P+VR A+ E+FKV+++GKSK+ +WKRM+TK TFVG+ FTR+P K+ERFIRPMALR KK Sbjct: 121 LPQVRGVAEEEMFKVIRTGKSKKNSWKRMITKATFVGDGFTRRPVKYERFIRPMALRQKK 180 Query: 590 AHVTHPELKATFCLPIIGVKKNPSSQMYTSLGVITKGTVIEVNISE 727 A+VTH EL T LPIIGVKKNP S YT LGV+TKGTVIEVN+SE Sbjct: 181 ANVTHKELGVTMQLPIIGVKKNPQSPTYTQLGVLTKGTVIEVNVSE 226 >SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 230 Score = 29.1 bits (62), Expect = 0.68 Identities = 23/63 (36%), Positives = 30/63 (47%), Gaps = 2/63 (3%) Frame = -2 Query: 298 FGDLLCVMFDILLFVRFDFLFHLNLFIAAFLIEYF-SFDTAEFARLFSAFMRLTSF-TLP 125 F LL F +L F+ F F F F++ I YF SF F LF F+ + F + Sbjct: 113 FRCLLLFFFFLLFFLSFSFSFSFLFFLSQIFIVYFSSFPILHF--LFFFFLCVCVFLSFL 170 Query: 124 FSL 116 FSL Sbjct: 171 FSL 173 >SPAC23C4.14 |alg1||mannosyltransferase complex subunit Alg1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 424 Score = 28.3 bits (60), Expect = 1.2 Identities = 13/41 (31%), Positives = 18/41 (43%) Frame = +2 Query: 566 PMALRFKKAHVTHPELKATFCLPIIGVKKNPSSQMYTSLGV 688 PM F + HP K FC+P + ++ P LGV Sbjct: 287 PMKEEFSQYIKKHPLHKVRFCMPWLSIEDYPQVMACADLGV 327 >SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|chr 1|||Manual Length = 161 Score = 27.9 bits (59), Expect = 1.6 Identities = 22/84 (26%), Positives = 45/84 (53%) Frame = +2 Query: 209 ERRNEKIQMKKKIKAHEEKNVKHNTEKVAEGALPVYLLDRDVQSRAKVLSNMIKQKRKEK 388 E+ + K+++ ++ +K ++ TEK+ + + +R VQS + +M +QK +E Sbjct: 82 EQLSRKVELLEEELETNDKLLRETTEKMRQTDVKAEHFERRVQSLERERDDM-EQKLEEM 140 Query: 389 AGKWDVPIPKVRAQADAEVFKVLK 460 K+ KV+A+ D EV + L+ Sbjct: 141 TDKY----TKVKAELD-EVHQALE 159 >SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1233 Score = 26.6 bits (56), Expect = 3.6 Identities = 11/46 (23%), Positives = 26/46 (56%) Frame = +2 Query: 425 AQADAEVFKVLKSGKSKRKAWKRMVTKVTFVGENFTRKPPKFERFI 562 A +++ K+L + K+ KR+V+++T + N T + +R++ Sbjct: 883 ASENSKTEKILLAASEKKLVGKRLVSELTKLSGNITLLESEIDRYV 928 >SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 26.6 bits (56), Expect = 3.6 Identities = 16/42 (38%), Positives = 19/42 (45%), Gaps = 1/42 (2%) Frame = -2 Query: 259 FVRFD-FLFHLNLFIAAFLIEYFSFDTAEFARLFSAFMRLTS 137 FV D FL+ LF LI +F+ F LFS F S Sbjct: 171 FVSVDSFLYIFTLFPIRVLISFFTLSRCIFQGLFSTFFHRNS 212 >SPAC22H12.03 |||mitochondrial hydrolase|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 25.8 bits (54), Expect = 6.3 Identities = 13/45 (28%), Positives = 23/45 (51%) Frame = +2 Query: 278 NTEKVAEGALPVYLLDRDVQSRAKVLSNMIKQKRKEKAGKWDVPI 412 N K +E + +++D+ R+ +LSN+ K K+ VPI Sbjct: 145 NITKYSEADKMMSTVEKDILVRSFLLSNLKKDSNNSNTFKFRVPI 189 >SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 764 Score = 25.4 bits (53), Expect = 8.4 Identities = 10/36 (27%), Positives = 22/36 (61%) Frame = +2 Query: 206 KERRNEKIQMKKKIKAHEEKNVKHNTEKVAEGALPV 313 + + NE + + + ++ E+N NTE++AE + P+ Sbjct: 98 ENQENEADEAENEETSYSEQNHTENTEEIAEESRPL 133 >SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism |Schizosaccharomyces pombe|chr 2|||Manual Length = 325 Score = 25.4 bits (53), Expect = 8.4 Identities = 8/13 (61%), Positives = 12/13 (92%) Frame = +2 Query: 53 MPQNEYIERHQKL 91 +PQ EY+E+H+KL Sbjct: 195 LPQTEYLEKHKKL 207 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,645,660 Number of Sequences: 5004 Number of extensions: 50811 Number of successful extensions: 169 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 164 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 167 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 343230174 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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