BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc10c05
(651 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g25940.1 68416.m03233 transcription factor S-II (TFIIS) domai... 66 3e-11
At4g07950.1 68417.m01275 DNA-directed RNA polymerase III family ... 54 6e-08
At1g01210.1 68414.m00035 DNA-directed RNA polymerase III family ... 54 6e-08
At2g38560.1 68415.m04737 transcription factor S-II (TFIIS) domai... 42 3e-04
At3g44850.1 68416.m04832 protein kinase-related contains eukaryo... 29 2.7
At4g19510.2 68417.m02870 disease resistance protein (TIR-NBS-LRR... 28 6.2
At4g19510.1 68417.m02869 disease resistance protein (TIR-NBS-LRR... 28 6.2
At4g12170.1 68417.m01934 thioredoxin family protein similar to S... 27 8.2
At2g25565.1 68415.m03060 hypothetical protein 27 8.2
At1g55240.1 68414.m06309 expressed protein contains Pfam profile... 27 8.2
>At3g25940.1 68416.m03233 transcription factor S-II (TFIIS)
domain-containing protein similar to SP|O94703
DNA-directed RNA polymerase I 13.1 kDa polypeptide (EC
2.7.7.6) {Schizosaccharomyces pombe}; contains Pfam
profile PF01096: Transcription factor S-II (TFIIS)
Length = 119
Score = 65.7 bits (153), Expect = 3e-11
Identities = 36/109 (33%), Positives = 56/109 (51%), Gaps = 3/109 (2%)
Frame = +1
Query: 55 FCARCGSILPLLQEFGFVKCYTCKAHYEADNYSNLKFQYTIHFNTISVITNENIL--HTD 228
FC CG++L +L+ + +C CK A + + + YT+ I ++ T
Sbjct: 11 FCNLCGTML-VLKSTKYAECPHCKTTRNAKDIIDKEIAYTVSAEDIRRELGISLFGEKTQ 69
Query: 229 GP-EGPVVERKCAKCGYDRMSYATLQLRSADEGQTVFYTCIKCKYKETE 372
E P +++ C KC + + Y T Q RSADEGQT +YTC C ++ TE
Sbjct: 70 AEAELPKIKKACEKCQHPELVYTTRQTRSADEGQTTYYTCPNCAHRFTE 118
>At4g07950.1 68417.m01275 DNA-directed RNA polymerase III family
protein similar to SP|Q9Y2Y1 DNA-directed RNA
polymerases III 12.5 kDa polypeptide (EC 2.7.7.6) (RNA
polymerase III C11 subunit) {Homo sapiens}; contains
Pfam profiles PF02150: RNA polymerases M/15 Kd subunit,
PF01096: Transcription factor S-II (TFIIS)
Length = 106
Score = 54.4 bits (125), Expect = 6e-08
Identities = 31/105 (29%), Positives = 55/105 (52%), Gaps = 3/105 (2%)
Frame = +1
Query: 55 FCARCGSILPLLQEFGFVK--CYTCKAHYEADNYSNLKFQYTIHFNTIS-VITNENILHT 225
FC CG++L + G + C TC + +K + + +I V+T ++I
Sbjct: 3 FCPTCGNLLRY-EGGGSSRFFCSTCPYVANIERRVEIKKKQLLVKKSIEPVVTKDDI--- 58
Query: 226 DGPEGPVVERKCAKCGYDRMSYATLQLRSADEGQTVFYTCIKCKY 360
P E C +CG+D+ + ++Q+RSADE ++ FY C+KC++
Sbjct: 59 --PTAAETEAPCPRCGHDKAYFKSMQIRSADEPESRFYRCLKCEF 101
>At1g01210.1 68414.m00035 DNA-directed RNA polymerase III family
protein similar to SP|O13896 DNA-directed RNA
polymerases III 12.5 kDa polypeptide (EC 2.7.7.6)
{Schizosaccharomyces pombe}; contains Pfam profiles
PF02150: RNA polymerases M/15 Kd subunit, PF01096:
Transcription factor S-II (TFIIS)
Length = 106
Score = 54.4 bits (125), Expect = 6e-08
Identities = 32/105 (30%), Positives = 54/105 (51%), Gaps = 3/105 (2%)
Frame = +1
Query: 55 FCARCGSILPLLQEFGFVK--CYTCK-AHYEADNYSNLKFQYTIHFNTISVITNENILHT 225
FC CG++L + G + C TC Y K Q + + +V+T ++I
Sbjct: 3 FCPTCGNLLRY-EGGGNSRFFCSTCPYVAYIQRQVEIKKKQLLVKKSIEAVVTKDDI--- 58
Query: 226 DGPEGPVVERKCAKCGYDRMSYATLQLRSADEGQTVFYTCIKCKY 360
P E C +CG+D+ + ++Q+RSADE ++ FY C+KC++
Sbjct: 59 --PTAAETEAPCPRCGHDKAYFKSMQIRSADEPESRFYRCLKCEF 101
>At2g38560.1 68415.m04737 transcription factor S-II (TFIIS)
domain-containing protein similar to SP|P49373
Transcription elongation factor S-II (TFIIS)
{Schizosaccharomyces pombe}; contains Pfam profile
PF01096: Transcription factor S-II (TFIIS)
Length = 378
Score = 42.3 bits (95), Expect = 3e-04
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = +1
Query: 256 KCAKCGYDRMSYATLQLRSADEGQTVFYTCIKC 354
KC +CG + +Y +Q RSADE T + TC+ C
Sbjct: 339 KCGRCGQRKCTYYQMQTRSADEPMTTYVTCVNC 371
>At3g44850.1 68416.m04832 protein kinase-related contains eukaryotic
protein kinase domain, INTERPRO:IPR000719
Length = 534
Score = 29.1 bits (62), Expect = 2.7
Identities = 20/54 (37%), Positives = 29/54 (53%)
Frame = -2
Query: 317 SADRNCRVAYDILS*PHFAHFLSTTGPSGPSVCSIFSFVITDIVLKCIVY*NFR 156
S D+ C V +L HF H TGP+G VC +F + + D +L I Y ++R
Sbjct: 101 SGDKKCVVK--LLD--HFKH----TGPNGKHVCMVFEY-LGDNLLSVIKYSDYR 145
>At4g19510.2 68417.m02870 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1049
Score = 27.9 bits (59), Expect = 6.2
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +1
Query: 436 IVSESLYAKISFTDCFKLGKFNKD 507
+V+E ++ FTDCFKL + K+
Sbjct: 853 LVTERMHTTFIFTDCFKLNQAEKE 876
>At4g19510.1 68417.m02869 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1049
Score = 27.9 bits (59), Expect = 6.2
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +1
Query: 436 IVSESLYAKISFTDCFKLGKFNKD 507
+V+E ++ FTDCFKL + K+
Sbjct: 853 LVTERMHTTFIFTDCFKLNQAEKE 876
>At4g12170.1 68417.m01934 thioredoxin family protein similar to
SP|Q9SEU6 Thioredoxin M-type 4, chloroplast precursor
(TRX-M4) {Arabidopsis thaliana}; contains Pfam profile:
PF00085 Thioredoxin
Length = 128
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/49 (28%), Positives = 24/49 (48%), Gaps = 1/49 (2%)
Frame = +1
Query: 58 CARCGSILPLLQEFGFVKCYTCKAH-YEADNYSNLKFQYTIHFNTISVI 201
CA CGS++P L+ Y K + + D L Y I ++ I+++
Sbjct: 54 CAECGSLMPELEFLDSEYEYMLKFYTVDTDEELELAKDYRIEYHPITIV 102
>At2g25565.1 68415.m03060 hypothetical protein
Length = 206
Score = 27.5 bits (58), Expect = 8.2
Identities = 8/16 (50%), Positives = 12/16 (75%)
Frame = +2
Query: 245 LWRENAQNAVTTECHM 292
LW +N +N +TT CH+
Sbjct: 178 LWNDNDKNQITTNCHI 193
>At1g55240.1 68414.m06309 expressed protein contains Pfam profile
PF04819: Family of unknown function (DUF716) (Plant
viral-response family)
Length = 311
Score = 27.5 bits (58), Expect = 8.2
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +1
Query: 106 VKCYTCKAHYEADNYSNLKFQYTIHFNTISVIT 204
VKC T +A + A + NL+F + NT+ V+T
Sbjct: 223 VKCSTEEALHRAKSLVNLEFSWLFVTNTLFVVT 255
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,271,528
Number of Sequences: 28952
Number of extensions: 259296
Number of successful extensions: 567
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 554
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 567
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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