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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc10b02
         (547 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g49910.1 68416.m05456 60S ribosomal protein L26 (RPL26A) 60S ...   192   2e-49
At5g67510.1 68418.m08513 60S ribosomal protein L26 (RPL26B)           189   1e-48
At1g28070.1 68414.m03436 expressed protein ; expression supporte...    28   3.5  
At4g35380.1 68417.m05026 guanine nucleotide exchange family prot...    27   6.2  
At1g50500.1 68414.m05664 membrane trafficking VPS53 family prote...    27   6.2  

>At3g49910.1 68416.m05456 60S ribosomal protein L26 (RPL26A) 60S
           RIBOSOMAL PROTEIN L26, Brassica rapa, EMBL:BRD495
          Length = 146

 Score =  192 bits (467), Expect = 2e-49
 Identities = 93/135 (68%), Positives = 116/135 (85%)
 Frame = +1

Query: 52  MKFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRG 231
           MK+N +VTSSRRKNRK HF+A S  RRV+MSSPLS +LRQK+NV+SMPIRKDDEVQ+VRG
Sbjct: 1   MKYNPRVTSSRRKNRKAHFTASSSERRVIMSSPLSTDLRQKYNVRSMPIRKDDEVQIVRG 60

Query: 232 HYKGQQVGKVMQVYRKKFVVYIERIQREKANGATAYVGIHPSKCVIVKLKMNKDRKAILD 411
            YKG++ GKV+QVYR+K+V++IERI REK NG T  VGI PSK VI KL+++KDRK++L+
Sbjct: 61  TYKGRE-GKVVQVYRRKWVIHIERITREKVNGTTVNVGIQPSKVVITKLRLDKDRKSLLE 119

Query: 412 RRAKGRLAALGKDKG 456
           R+AKGR AA  K+KG
Sbjct: 120 RKAKGR-AAADKEKG 133


>At5g67510.1 68418.m08513 60S ribosomal protein L26 (RPL26B)
          Length = 146

 Score =  189 bits (460), Expect = 1e-48
 Identities = 91/135 (67%), Positives = 116/135 (85%)
 Frame = +1

Query: 52  MKFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRG 231
           MKFN +V+SSRRKNRK HF+APS +RRVLMSSPLSK+LR K NV+SMPIRKDDEVQVVRG
Sbjct: 1   MKFNPRVSSSRRKNRKAHFTAPSSVRRVLMSSPLSKDLRNKHNVRSMPIRKDDEVQVVRG 60

Query: 232 HYKGQQVGKVMQVYRKKFVVYIERIQREKANGATAYVGIHPSKCVIVKLKMNKDRKAILD 411
            +KG++ GKVMQVYR+K+V++IERI REK NG+T  VG++ S  +I KL+++KDRK++L+
Sbjct: 61  TFKGRE-GKVMQVYRRKWVIHIERITREKVNGSTVNVGVNASNVMITKLRLDKDRKSLLE 119

Query: 412 RRAKGRLAALGKDKG 456
           R+A GR AA  K+KG
Sbjct: 120 RKANGR-AAADKEKG 133


>At1g28070.1 68414.m03436 expressed protein ; expression supported
           by MPSS
          Length = 176

 Score = 28.3 bits (60), Expect = 3.5
 Identities = 17/61 (27%), Positives = 28/61 (45%)
 Frame = +1

Query: 76  SSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRGHYKGQQVG 255
           S R      HFS PSH+ + L     + E+  +     M +++D    + RG+Y  Q  G
Sbjct: 38  SDRFLELSSHFSVPSHLEQCL--DLKTGEIYYRSWNSGMRVKEDPRKSMSRGNYADQSSG 95

Query: 256 K 258
           +
Sbjct: 96  E 96


>At4g35380.1 68417.m05026 guanine nucleotide exchange family protein
            similar to guanine nucleotide exchange factor [Homo
            sapiens] GI:5456754; contains Pfam profile PF01369: Sec7
            domain
          Length = 1706

 Score = 27.5 bits (58), Expect = 6.2
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +2

Query: 77   PQEGKTGRGISVLLHI 124
            P +G TG GISVLLH+
Sbjct: 1408 PFQGSTGSGISVLLHL 1423


>At1g50500.1 68414.m05664 membrane trafficking VPS53 family protein
           contains Pfam domain PF04100: Vps53-like, N-terminal
          Length = 798

 Score = 27.5 bits (58), Expect = 6.2
 Identities = 17/56 (30%), Positives = 27/56 (48%), Gaps = 2/56 (3%)
 Frame = +1

Query: 157 KELRQKFNVKSMPIR--KDDEVQVVRGHYKGQQVGKVMQVYRKKFVVYIERIQREK 318
           KEL +KF    +P +  +DD  ++       Q + K+ + Y KKF    E  + EK
Sbjct: 315 KELEKKFG-GGVPTKDIEDDIEEIGTWEDNSQNISKIRKKYEKKFAASQETEENEK 369


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,228,882
Number of Sequences: 28952
Number of extensions: 241908
Number of successful extensions: 690
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 679
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 688
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1023490624
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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