Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc10a21
(692 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q9VU37 Cluster: CG11268-PA; n=2; Sophophora|Rep: CG1126... 81 3e-14
UniRef50_UPI0000D5637F Cluster: PREDICTED: similar to Protein-S-... 66 1e-09
UniRef50_Q16NH8 Cluster: Protein-s isoprenylcysteine o-methyltra... 65 1e-09
UniRef50_O60725 Cluster: Protein-S-isoprenylcysteine O-methyltra... 61 2e-08
UniRef50_Q4T319 Cluster: Chromosome undetermined SCAF10132, whol... 51 2e-05
UniRef50_UPI00015B4861 Cluster: PREDICTED: similar to protein-s ... 51 3e-05
UniRef50_A7RLB3 Cluster: Predicted protein; n=2; Nematostella ve... 47 4e-04
UniRef50_P91266 Cluster: Putative uncharacterized protein; n=3; ... 42 0.019
UniRef50_UPI0000E4A984 Cluster: PREDICTED: hypothetical protein,... 40 0.076
UniRef50_Q5DD83 Cluster: SJCHGC05076 protein; n=2; Schistosoma j... 36 0.71
UniRef50_P32584 Cluster: Protein-S-isoprenylcysteine O-methyltra... 36 1.2
UniRef50_Q6FUK2 Cluster: Candida glabrata strain CBS138 chromoso... 35 1.6
UniRef50_Q02XA7 Cluster: Putative uncharacterized protein; n=1; ... 34 3.8
UniRef50_A1VCG8 Cluster: Peptidase A24A, prepilin type IV; n=3; ... 34 3.8
UniRef50_UPI000065FB41 Cluster: Protein-S-isoprenylcysteine O-me... 33 6.6
UniRef50_Q74HV7 Cluster: Putative uncharacterized protein; n=1; ... 33 8.8
>UniRef50_Q9VU37 Cluster: CG11268-PA; n=2; Sophophora|Rep:
CG11268-PA - Drosophila melanogaster (Fruit fly)
Length = 299
Score = 81.0 bits (191), Expect = 3e-14
Identities = 35/117 (29%), Positives = 61/117 (52%), Gaps = 1/117 (0%)
Frame = +2
Query: 344 NICPAGKQAIIWFMLTVCXXXXXXXXXXXXXXTSEIWALTYWGPSLYFCLLNFILRYAYK 523
++C G+ ++ F++T ++W WGP LY+ L+N I+R+ +
Sbjct: 16 SLCSEGRISLYCFLITAALVLIPSVPQNLYGVVPQVWGAVLWGPFLYYALINMIIRFVLR 75
Query: 524 GFLYEVSIRAAFLGAVFTIGLYLSTF-EDGIKVFGLYTMVLSMFHFSEFMSVALTNP 691
Y+V+IRA+FLG + + + F + FG Y +S+FH+SEF+ +A NP
Sbjct: 76 NHDYQVAIRASFLGFAMAVSVLVICFAPTEWQQFGAYGCFMSLFHYSEFLVIAFANP 132
>UniRef50_UPI0000D5637F Cluster: PREDICTED: similar to
Protein-S-isoprenylcysteine O-methyltransferase
(Isoprenylcysteine carboxylmethyltransferase)
(Prenylcysteine carboxyl methyltransferase) (pcCMT)
(Prenylated protein carboxyl methyltransferase) (PPMT)
isoform 2; n=1; Tribolium castaneum|Rep: PREDICTED:
similar to Protein-S-isoprenylcysteine
O-methyltransferase (Isoprenylcysteine
carboxylmethyltransferase) (Prenylcysteine carboxyl
methyltransferase) (pcCMT) (Prenylated protein carboxyl
methyltransferase) (PPMT) isoform 2 - Tribolium
castaneum
Length = 281
Score = 65.7 bits (153), Expect = 1e-09
Identities = 29/74 (39%), Positives = 49/74 (66%), Gaps = 3/74 (4%)
Frame = +2
Query: 479 LYFCLLN--FILRYAYKGFLYEVSIRAAFLGAVFTIGLYLS-TFEDGIKVFGLYTMVLSM 649
++F +N F L++ Y F + ++IRAAFLG V +GLY+ I++FG Y V+++
Sbjct: 42 VFFYAVNVYFFLKFFYNEFAFAIAIRAAFLGLVLVLGLYIKLVAPPNIQIFGGYMSVMAL 101
Query: 650 FHFSEFMSVALTNP 691
FH+SEF+++A+ P
Sbjct: 102 FHYSEFLAIAIVQP 115
>UniRef50_Q16NH8 Cluster: Protein-s isoprenylcysteine
o-methyltransferase; n=3; Endopterygota|Rep: Protein-s
isoprenylcysteine o-methyltransferase - Aedes aegypti
(Yellowfever mosquito)
Length = 362
Score = 65.3 bits (152), Expect = 1e-09
Identities = 26/72 (36%), Positives = 48/72 (66%), Gaps = 1/72 (1%)
Frame = +2
Query: 479 LYFCLLNFILRYAYKGFLYEVSIRAAFLGAVFTIG-LYLSTFEDGIKVFGLYTMVLSMFH 655
+Y+ +LN ++R + Y++++RA FLGAVF++G + + K FG+Y ++S+FH
Sbjct: 47 VYYLVLNVVIRLKFNTKDYQIAVRATFLGAVFSLGCIIFQNCTEEYKSFGVYVTLMSVFH 106
Query: 656 FSEFMSVALTNP 691
+SE++ +A NP
Sbjct: 107 YSEYLGIAFCNP 118
>UniRef50_O60725 Cluster: Protein-S-isoprenylcysteine
O-methyltransferase; n=29; Deuterostomia|Rep:
Protein-S-isoprenylcysteine O-methyltransferase - Homo
sapiens (Human)
Length = 284
Score = 61.3 bits (142), Expect = 2e-08
Identities = 31/74 (41%), Positives = 43/74 (58%)
Frame = +2
Query: 470 GPSLYFCLLNFILRYAYKGFLYEVSIRAAFLGAVFTIGLYLSTFEDGIKVFGLYTMVLSM 649
G +LY LN +L Y+ Y+++IRA FLG VF G LS + FG Y LS+
Sbjct: 43 GLALYVAGLNALLLLLYRPPRYQIAIRACFLGFVFGCGTLLSFSQSSWSHFGWYMCSLSL 102
Query: 650 FHFSEFMSVALTNP 691
FH+SE++ A+ NP
Sbjct: 103 FHYSEYLVTAVNNP 116
>UniRef50_Q4T319 Cluster: Chromosome undetermined SCAF10132, whole
genome shotgun sequence; n=3; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF10132,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 401
Score = 51.2 bits (117), Expect = 2e-05
Identities = 25/54 (46%), Positives = 32/54 (59%)
Frame = +2
Query: 530 LYEVSIRAAFLGAVFTIGLYLSTFEDGIKVFGLYTMVLSMFHFSEFMSVALTNP 691
L EV++RA FLG F GL +S E FG Y LS FH+SE++ A+ NP
Sbjct: 79 LGEVAVRACFLGVTFGCGLIISFSESTWTHFGWYMCSLSFFHYSEYLVTAIINP 132
>UniRef50_UPI00015B4861 Cluster: PREDICTED: similar to protein-s
isoprenylcysteine o-methyltransferase; n=1; Nasonia
vitripennis|Rep: PREDICTED: similar to protein-s
isoprenylcysteine o-methyltransferase - Nasonia
vitripennis
Length = 308
Score = 50.8 bits (116), Expect = 3e-05
Identities = 24/52 (46%), Positives = 33/52 (63%), Gaps = 1/52 (1%)
Frame = +2
Query: 539 VSIRAAFLGAVFTIGLYLSTFEDGI-KVFGLYTMVLSMFHFSEFMSVALTNP 691
+S RA FLG ++G+ + I K FG+Y VLS FHF+EF+ +A TNP
Sbjct: 41 ISSRANFLGFCLSLGILICNSSIYIWKTFGIYVTVLSTFHFTEFLGIAFTNP 92
>UniRef50_A7RLB3 Cluster: Predicted protein; n=2; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 284
Score = 47.2 bits (107), Expect = 4e-04
Identities = 23/51 (45%), Positives = 31/51 (60%)
Frame = +2
Query: 539 VSIRAAFLGAVFTIGLYLSTFEDGIKVFGLYTMVLSMFHFSEFMSVALTNP 691
V+ RA FLG+VF I + L ++ G Y LS FHFSE+M V++ NP
Sbjct: 61 VAKRAGFLGSVFGISVVLIVNSSILRYLGWYLASLSFFHFSEYMMVSIYNP 111
>UniRef50_P91266 Cluster: Putative uncharacterized protein; n=3;
Caenorhabditis|Rep: Putative uncharacterized protein -
Caenorhabditis elegans
Length = 295
Score = 41.5 bits (93), Expect = 0.019
Identities = 21/53 (39%), Positives = 31/53 (58%), Gaps = 2/53 (3%)
Frame = +2
Query: 536 EVSIRAAFLGAVFTIGLYLSTFEDGI--KVFGLYTMVLSMFHFSEFMSVALTN 688
++ + AAFLG + L + +G + F Y + LS+FHFSEF+ ALTN
Sbjct: 76 QILMPAAFLGCAVAVNLVYTVAHEGELWEYFSRYFLFLSVFHFSEFVFTALTN 128
>UniRef50_UPI0000E4A984 Cluster: PREDICTED: hypothetical protein,
partial; n=3; Strongylocentrotus purpuratus|Rep:
PREDICTED: hypothetical protein, partial -
Strongylocentrotus purpuratus
Length = 159
Score = 39.5 bits (88), Expect = 0.076
Identities = 18/50 (36%), Positives = 26/50 (52%)
Frame = +2
Query: 539 VSIRAAFLGAVFTIGLYLSTFEDGIKVFGLYTMVLSMFHFSEFMSVALTN 688
V+ + LG + GL + + FG Y ++LS FHFSEF S + N
Sbjct: 1 VAFQGCLLGVLSGTGLIIGFSSNTFHPFGWYLVILSFFHFSEFFSTCIYN 50
>UniRef50_Q5DD83 Cluster: SJCHGC05076 protein; n=2; Schistosoma
japonicum|Rep: SJCHGC05076 protein - Schistosoma
japonicum (Blood fluke)
Length = 298
Score = 36.3 bits (80), Expect = 0.71
Identities = 21/53 (39%), Positives = 30/53 (56%), Gaps = 6/53 (11%)
Frame = +2
Query: 548 RAAFLGAVFTIGLYL---STF---EDGIKVFGLYTMVLSMFHFSEFMSVALTN 688
RA FLG VF +GL+ S F + FG+Y L++FH+SEF ++ N
Sbjct: 73 RAYFLGCVFGLGLFFALTSVFMNVHNDFCTFGIYLDFLALFHWSEFYFTSIYN 125
>UniRef50_P32584 Cluster: Protein-S-isoprenylcysteine
O-methyltransferase; n=4; Saccharomycetaceae|Rep:
Protein-S-isoprenylcysteine O-methyltransferase -
Saccharomyces cerevisiae (Baker's yeast)
Length = 239
Score = 35.5 bits (78), Expect = 1.2
Identities = 19/55 (34%), Positives = 32/55 (58%), Gaps = 1/55 (1%)
Frame = +2
Query: 530 LYEVSIRAAFLGAVFTIGLYLSTFED-GIKVFGLYTMVLSMFHFSEFMSVALTNP 691
L+EV++ + LG + +G+++ F K F L+ + LS+FHF E+ A NP
Sbjct: 20 LHEVTMTSYILGIL--LGIFVGLFPQIRFKNFNLFIIALSLFHFLEYYITAKYNP 72
>UniRef50_Q6FUK2 Cluster: Candida glabrata strain CBS138 chromosome
F complete sequence; n=2; Saccharomycetales|Rep: Candida
glabrata strain CBS138 chromosome F complete sequence -
Candida glabrata (Yeast) (Torulopsis glabrata)
Length = 254
Score = 35.1 bits (77), Expect = 1.6
Identities = 19/54 (35%), Positives = 30/54 (55%)
Frame = +2
Query: 530 LYEVSIRAAFLGAVFTIGLYLSTFEDGIKVFGLYTMVLSMFHFSEFMSVALTNP 691
L ++++ + LG + I + L TF + F +Y + LS+FHF EF A NP
Sbjct: 34 LDDIAVTSFALGILLGIFVGLLTFTQ-FRNFNVYIIALSIFHFLEFYVTAKVNP 86
>UniRef50_Q02XA7 Cluster: Putative uncharacterized protein; n=1;
Lactococcus lactis subsp. cremoris SK11|Rep: Putative
uncharacterized protein - Lactococcus lactis subsp.
cremoris (strain SK11)
Length = 168
Score = 33.9 bits (74), Expect = 3.8
Identities = 21/80 (26%), Positives = 41/80 (51%), Gaps = 2/80 (2%)
Frame = -3
Query: 501 KFNKQKYNEGPQ*VSAQISDVNPKRLPEKNDMKN--IQTVSINHIIACFPAGHMFFIVKT 328
+F K+KY + + + QI ++ + ++ N + T SIN+II C AG + + +
Sbjct: 70 RFYKKKYLKKQKEIIEQIQEILKSEIIVNAELPNDYMNTKSINYIIGCLEAGEVSNLKEA 129
Query: 327 SIIKDACSRE*FSRLLFKTD 268
+ + + SR+ L KT+
Sbjct: 130 TNLLELESRDSHVHYLIKTE 149
>UniRef50_A1VCG8 Cluster: Peptidase A24A, prepilin type IV; n=3;
Desulfovibrio|Rep: Peptidase A24A, prepilin type IV -
Desulfovibrio vulgaris subsp. vulgaris (strain DP4)
Length = 188
Score = 33.9 bits (74), Expect = 3.8
Identities = 21/62 (33%), Positives = 33/62 (53%)
Frame = +2
Query: 491 LLNFILRYAYKGFLYEVSIRAAFLGAVFTIGLYLSTFEDGIKVFGLYTMVLSMFHFSEFM 670
LL FILR G + ++ AFLGA + ++ T G GLY +V+ +FH + M
Sbjct: 66 LLPFILRVMGAGDVKLMAAAGAFLGARGVLSAFIWTSLAG----GLYALVVLLFHLPQLM 121
Query: 671 SV 676
++
Sbjct: 122 AI 123
>UniRef50_UPI000065FB41 Cluster: Protein-S-isoprenylcysteine
O-methyltransferase (EC 2.1.1.100) (Isoprenylcysteine
carboxylmethyltransferase) (Prenylcysteine carboxyl
methyltransferase) (pcCMT) (Prenylated protein carboxyl
methyltransferase) (PPMT).; n=1; Takifugu rubripes|Rep:
Protein-S-isoprenylcysteine O-methyltransferase (EC
2.1.1.100) (Isoprenylcysteine carboxylmethyltransferase)
(Prenylcysteine carboxyl methyltransferase) (pcCMT)
(Prenylated protein carboxyl methyltransferase) (PPMT).
- Takifugu rubripes
Length = 284
Score = 33.1 bits (72), Expect = 6.6
Identities = 23/72 (31%), Positives = 34/72 (47%), Gaps = 1/72 (1%)
Frame = +2
Query: 479 LYFCLLNFILRYAYKGFLYEVSIRA-AFLGAVFTIGLYLSTFEDGIKVFGLYTMVLSMFH 655
L+ + N +L Y+G LY+VS F + LY I Y LS+FH
Sbjct: 53 LHTAVTNGLLLIIYRGPLYKVSFDVRGFPLKLLIAHLYFII----IHFHNRYMCSLSLFH 108
Query: 656 FSEFMSVALTNP 691
+SE++ A+ NP
Sbjct: 109 YSEYLVTAIINP 120
>UniRef50_Q74HV7 Cluster: Putative uncharacterized protein; n=1;
Lactobacillus johnsonii|Rep: Putative uncharacterized
protein - Lactobacillus johnsonii
Length = 169
Score = 32.7 bits (71), Expect = 8.8
Identities = 20/58 (34%), Positives = 31/58 (53%), Gaps = 1/58 (1%)
Frame = -3
Query: 189 IFITVTDTMT-IYCS*LEWVN*NKNQINNTKLLKSNNQ*YIFYTFCWFLQIFINKCIC 19
+ + +TD + YC W+N NK +NN K LK+ FY + +F Q+F+ IC
Sbjct: 71 VIVAITDFLLGYYC----WINKNK-LVNNIKALKT------FYIWQFFCQLFVGNIIC 117
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 650,928,703
Number of Sequences: 1657284
Number of extensions: 12948980
Number of successful extensions: 26740
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 25637
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 26726
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 54545459628
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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