Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt8j06
         (576 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p...   181   6e-47
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo...    75   6e-15
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ...    73   3e-14
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d...    56   3e-09
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase...    55   9e-09
SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe...    29   0.64 
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc...    25   6.0  
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc...    25   6.0  

>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 360

 Score =  181 bits (441), Expect = 6e-47
 Identities = 80/170 (47%), Positives = 112/170 (65%), Gaps = 1/170 (0%)
 Frame = +1

Query: 52  MATDNLTALLYKPNDLRLVQTPIPEISED-EVLLRMDCVGICGSDVHYWQKGQCGHFVLE 228
           MA      +L K  D  +   P   +++D +V + +   GICGSDVHYW++G  G F+L+
Sbjct: 1   MAPAEKAFVLRKKMDTAIEDRPGQTLTDDHQVKVAIKATGICGSDVHYWKEGGIGDFILK 60

Query: 229 EPMIMGHEASGVVAKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLCPDMIFCATP 408
           +PMI+GHE++GVV ++G  V +L  GD VA+EPG  CR C++C++GRY+LCP M F ATP
Sbjct: 61  KPMILGHESAGVVVEVGKGVSSLKPGDPVAVEPGCVCRLCDYCRSGRYNLCPHMEFAATP 120

Query: 409 PVHGNLVRYYKHAADFCFKLPDHVTMEEGALLEPLAVGIHACKRGGVSAG 558
           P  G L  YY    DFC KLP  +++EEGAL EP++V +HA  RG +  G
Sbjct: 121 PYDGTLRTYYITTEDFCTKLPKQISVEEGALFEPMSVAVHAMTRGNLKCG 170


>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
           Adh1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 350

 Score = 75.4 bits (177), Expect = 6e-15
 Identities = 45/140 (32%), Positives = 71/140 (50%), Gaps = 3/140 (2%)
 Frame = +1

Query: 88  PNDLRLVQTPIPEISEDEVLLRMDCVGICGSDVHYWQKGQCGHFVL--EEPMIMGHEASG 261
           P +++  + P+ E  +DEVL+ +   G+C +D+H  Q    G + L  + P+I GHE +G
Sbjct: 18  PENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQ----GDWPLPAKMPLIGGHEGAG 73

Query: 262 VVAKIGSKVKNLTVGDRVAIE-PGVPCRYCEFCKTGRYHLCPDMIFCATPPVHGNLVRYY 438
           VV K+G+ V  L +GDRV ++     C  CE+C      +CP  I  +   V G    Y 
Sbjct: 74  VVVKVGAGVTRLKIGDRVGVKWMNSSCGNCEYCMKAEETICPH-IQLSGYTVDGTFQHYC 132

Query: 439 KHAADFCFKLPDHVTMEEGA 498
              A     +P+ V +E  A
Sbjct: 133 IANATHATIIPESVPLEVAA 152


>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 423

 Score = 72.9 bits (171), Expect = 3e-14
 Identities = 55/181 (30%), Positives = 80/181 (44%), Gaps = 24/181 (13%)
 Frame = +1

Query: 88  PNDLRLVQTPIPEISED-EVLLRMDCVGIC-GSDVHYWQKGQCGHFVLEEPMIMGHEASG 261
           P ++++ + P P I+   +V+++     IC GSD H +     G   +E+  I+GHE+ G
Sbjct: 45  PLNVKIAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPG---IEKGAILGHESCG 101

Query: 262 VVAKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLCPDMIFCATPPV-----HGNL 426
           +VA+ G +V NL +GDRV I   + C  C FCK   Y  C          V     H  +
Sbjct: 102 IVAEKGDEVNNLEIGDRVVIAFDLACGQCSFCKRHEYAACDTTNDSKLMDVNYGSHHSAI 161

Query: 427 VRYYKHAADF----------------CFKLPDHVTMEEGALL-EPLAVGIHACKRGGVSA 555
             Y K   D                 C KLPD +   EG  + + L   +HAC  G V  
Sbjct: 162 FGYTKLLGDVPGCQAEYIRVPFAEINCCKLPDDIPDSEGLFMSDVLCTSLHACTLGEVKK 221

Query: 556 G 558
           G
Sbjct: 222 G 222


>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
           dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 380

 Score = 56.4 bits (130), Expect = 3e-09
 Identities = 32/98 (32%), Positives = 53/98 (54%)
 Frame = +1

Query: 88  PNDLRLVQTPIPEISEDEVLLRMDCVGICGSDVHYWQKGQCGHFVLEEPMIMGHEASGVV 267
           P  +  VQ   P + E  V +++   G+C +D  Y   G+    +   P+I+GHE +G+V
Sbjct: 24  PLSIENVQVFPPRVHE--VRIKIVNSGVCHTDA-YTLSGKDPEGLF--PVILGHEGAGIV 78

Query: 268 AKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLC 381
             +G +V  + VGD V       C+ C+FCK+G+ +LC
Sbjct: 79  ESVGPQVTTVQVGDPVIALYTPECKTCKFCKSGKTNLC 116


>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score = 54.8 bits (126), Expect = 9e-09
 Identities = 27/81 (33%), Positives = 45/81 (55%)
 Frame = +1

Query: 139 EVLLRMDCVGICGSDVHYWQKGQCGHFVLEEPMIMGHEASGVVAKIGSKVKNLTVGDRVA 318
           EV +++D   +C +D  Y   G         P+++GHE +G+V  IG  V N+  GD V 
Sbjct: 36  EVRVKVDWSAVCHTDA-YTLSGVDPEGAF--PIVLGHEGAGIVESIGEGVINVRPGDHVI 92

Query: 319 IEPGVPCRYCEFCKTGRYHLC 381
           +     C+ C+FC++G+ +LC
Sbjct: 93  LLYTPECKECKFCRSGKTNLC 113


>SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 346

 Score = 28.7 bits (61), Expect = 0.64
 Identities = 10/31 (32%), Positives = 19/31 (61%)
 Frame = +1

Query: 223 LEEPMIMGHEASGVVAKIGSKVKNLTVGDRV 315
           L+ P++ G + +G++ K+G  V+    GD V
Sbjct: 58  LQLPVVPGSDGAGIIEKVGEDVEGFEKGDSV 88


>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 587

 Score = 25.4 bits (53), Expect = 6.0
 Identities = 11/31 (35%), Positives = 17/31 (54%)
 Frame = -1

Query: 483 GHVIRKLEAKISCVLVISDEVSVYWGRGAED 391
           GH ++ +EAK    + I  + SV  G+G  D
Sbjct: 211 GHTLKDMEAKSGAKIAIRGKGSVKEGKGRSD 241


>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1576

 Score = 25.4 bits (53), Expect = 6.0
 Identities = 11/20 (55%), Positives = 14/20 (70%)
 Frame = +1

Query: 103  LVQTPIPEISEDEVLLRMDC 162
            LVQ+ I E SED + L +DC
Sbjct: 1489 LVQSAIRESSEDAIALAVDC 1508


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,384,450
Number of Sequences: 5004
Number of extensions: 48140
Number of successful extensions: 137
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 246098644
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)