Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt8h12
         (614 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar...   195   5e-51
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi...    97   2e-21
SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces ...    30   0.31 
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch...    27   2.2  
SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    26   3.8  
SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein Rad4|Schizosac...    26   5.0  
SPAC1F3.01 |rrp6|SPAC3H8.11|exosome subunit Rrp6 |Schizosaccharo...    25   6.6  
SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|ch...    25   6.6  
SPAC22F3.13 |tsc1||hamartin|Schizosaccharomyces pombe|chr 1|||Ma...    25   8.7  
SPCC622.15c |||sequence orphan|Schizosaccharomyces pombe|chr 3||...    25   8.7  
SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni...    25   8.7  

>SPBC354.02c |sec61||translocon alpha subunit
           Sec61|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 479

 Score =  195 bits (475), Expect = 5e-51
 Identities = 84/147 (57%), Positives = 116/147 (78%), Gaps = 1/147 (0%)
 Frame = +2

Query: 158 IKFLEVIKPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADPF 337
           ++FL+++KPF   LPEIA PERK+ F++K+LWT +TL IFLV  Q+PL+GI+SSDS+DP 
Sbjct: 4   LRFLDLVKPFAPFLPEIAAPERKVPFKQKMLWTGVTLLIFLVMSQVPLYGIVSSDSSDPL 63

Query: 338 YWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAQKLFGM 514
            W+R+ILA+NRGTLMELGISPIVTS +++QLL G+++IEV  +   DR ++   QK   +
Sbjct: 64  LWLRMILAANRGTLMELGISPIVTSSMLVQLLVGSQLIEVNMELKSDREMYQLVQKFLAI 123

Query: 515 VITVGQAIVYVMTGMYGEPSEIGAGVC 595
           +I  GQA  YV+TGMYG P ++GAG+C
Sbjct: 124 IIAFGQATAYVLTGMYGRPQDLGAGIC 150


>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 475

 Score = 96.7 bits (230), Expect = 2e-21
 Identities = 50/144 (34%), Positives = 82/144 (56%), Gaps = 1/144 (0%)
 Frame = +2

Query: 155 GIKFLEVIKPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADP 334
           G +F+  IKP  S+LPE+  P+  ++  EK+ W A  + ++ +   IP++G   +D+ DP
Sbjct: 3   GARFINFIKPLSSLLPEVEGPKTHLELVEKLGWMAGCVVVYQILSIIPVYGAEKTDTLDP 62

Query: 335 FYWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAQKLFG 511
               RV+  S+   LM  G++PI  S  ++Q+LA  K I V  +   DR LF  AQK+  
Sbjct: 63  INNFRVLDGSSASGLMITGLAPIYLSSFLLQILASKKKIAVNFNLIIDRVLFQNAQKVVS 122

Query: 512 MVITVGQAIVYVMTGMYGEPSEIG 583
            ++ +  A+ YV +G YG  S++G
Sbjct: 123 ALLYLILAVTYVSSGYYGSFSDLG 146


>SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 346

 Score = 29.9 bits (64), Expect = 0.31
 Identities = 23/67 (34%), Positives = 30/67 (44%), Gaps = 4/67 (5%)
 Frame = -3

Query: 504 NFCAPLNKALSFGVSPTS-MILAPASNCMISPDVTIGEIPSSINV---PLFDARITRIQ* 337
           + CA +NKAL  G+  TS    A    C   PD  I  +  S  V   PL + RI  ++ 
Sbjct: 104 SLCAAINKALDAGIPITSPAFYADEKQC---PDTLIASVFDSATVEKIPLLEERIRIMRA 160

Query: 336 KGSALSD 316
            G  L D
Sbjct: 161 SGRVLVD 167


>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
            Mok12|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2352

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
 Frame = +2

Query: 158  IKFLEVI-KPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMS 316
            I+FL+   KP+ S    +    R++ +  K  WT   + I L+    PLFGI S
Sbjct: 1055 IRFLDPPPKPYLSYKMYLNDFTRQLHYIPKGSWTVQIVAIVLLILLPPLFGIFS 1108


>SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 340

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 17/61 (27%), Positives = 29/61 (47%)
 Frame = -3

Query: 384 SINVPLFDARITRIQ*KGSALSDDIIPNKGIWQHTKKINNVIAVHNTFSRN*ILRSGFAI 205
           S+ + LFD  I+ +    S +SD  IP K      +++ N  +   T+S N + +S    
Sbjct: 273 SLLLGLFDKSISMLNCHSSKISDGDIPYKSHQVFLQELQNKYSEIKTYSSNKLNKSSIKS 332

Query: 204 S 202
           S
Sbjct: 333 S 333


>SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein
           Rad4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 648

 Score = 25.8 bits (54), Expect = 5.0
 Identities = 9/23 (39%), Positives = 14/23 (60%)
 Frame = -3

Query: 207 ISGNILQNGLITSKNFIPILIYC 139
           + G  L +GL+  K+F+P L  C
Sbjct: 83  VQGEDLDDGLLVDKHFLPTLFKC 105


>SPAC1F3.01 |rrp6|SPAC3H8.11|exosome subunit Rrp6
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 777

 Score = 25.4 bits (53), Expect = 6.6
 Identities = 13/46 (28%), Positives = 22/46 (47%)
 Frame = -1

Query: 608 DNEEGTLRHQFHWVHHTFQSLHKQ*PDQQ*SPYQIISVHH*TKPCL 471
           D  +G L+      H +   +  + P ++  PY++I   H TKP L
Sbjct: 94  DKAQGLLKKPAIETHASTSDVANKKPKKEKLPYKVIHAAHLTKPQL 139


>SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 845

 Score = 25.4 bits (53), Expect = 6.6
 Identities = 15/56 (26%), Positives = 28/56 (50%)
 Frame = -3

Query: 507 NNFCAPLNKALSFGVSPTSMILAPASNCMISPDVTIGEIPSSINVPLFDARITRIQ 340
           N  C   +K     + P ++I +P+S    S ++   + PSS+NV   + R+  +Q
Sbjct: 368 NGLCRNDSKCRFLMILPETIIKSPSSFIGDSYNIANIDPPSSMNVQSSEFRVGEVQ 423


>SPAC22F3.13 |tsc1||hamartin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 899

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 10/27 (37%), Positives = 19/27 (70%)
 Frame = +3

Query: 36  LFSVKKSYWSVINEQKLNGY*RIDQLI 116
           L S+ K+ W+V++E++  GY  + +LI
Sbjct: 3   LQSLVKALWNVLHEEESEGYPDLTELI 29


>SPCC622.15c |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 557

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 11/21 (52%), Positives = 15/21 (71%)
 Frame = -3

Query: 420 ISPDVTIGEIPSSINVPLFDA 358
           IS ++T GE+PS  NVP  +A
Sbjct: 414 ISANMTTGEMPSMTNVPAGNA 434


>SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit
           Cct1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 556

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 12/44 (27%), Positives = 22/44 (50%)
 Frame = -3

Query: 303 NKGIWQHTKKINNVIAVHNTFSRN*ILRSGFAISGNILQNGLIT 172
           +KG  ++  K  N++  H   SR  +L  G+A++  I    + T
Sbjct: 189 SKGETRYPVKAVNILKAHGKSSRESVLVKGYALNCTIASQAMKT 232


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,535,569
Number of Sequences: 5004
Number of extensions: 51030
Number of successful extensions: 133
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 131
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 269634532
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)