Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt6o12
         (710 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC12C2.12c |glo1|SPBC21D10.03c|glyoxalase I |Schizosaccharomyc...    66   5e-12
SPAC1952.16 |rga9||RhoGAp, GTPase activator towards Rho/Rac/Cdc4...    27   2.6  
SPAC732.02c |||fructose-2,6-bisphosphate 2-phosphatase activity ...    26   4.6  
SPAC14C4.13 |rad17||RFC related checkpoint protein Rad17|Schizos...    26   6.1  
SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pom...    25   8.1  

>SPBC12C2.12c |glo1|SPBC21D10.03c|glyoxalase I |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 302

 Score = 66.1 bits (154), Expect = 5e-12
 Identities = 43/118 (36%), Positives = 62/118 (52%), Gaps = 6/118 (5%)
 Frame = +1

Query: 163 KKMAQGISPQE--IEALCQT---PDPTTKDFMFQQTMYRIKDPRKTIPFYTGVLGMTLLK 327
           K +A  + P    IE + Q+   P     +F F  TM R+KDP  +I FY   LGM ++ 
Sbjct: 135 KHIAFALDPDNYWIELVSQSETKPKANISNFRFNHTMVRVKDPEPSIAFYEK-LGMKVID 193

Query: 328 QLHFPAMKFSLFFMGYENPAEVPRDDDARTKWAMTRKATLELTYNWGTESDDSS-YHN 498
           +   P  KF+ +F+ Y  P+++PR D         R+  LELT+NWGTE +    YHN
Sbjct: 194 KADHPNGKFTNYFLAY--PSDLPRHD---------REGLLELTHNWGTEKESGPVYHN 240



 Score = 59.7 bits (138), Expect = 4e-10
 Identities = 35/93 (37%), Positives = 52/93 (55%)
 Frame = +1

Query: 205 LCQTPDPTTKDFMFQQTMYRIKDPRKTIPFYTGVLGMTLLKQLHFPAMKFSLFFMGYENP 384
           +  T D +T  +    TM R+KD  K++ FYT V GM L+ Q  F   +FSL F+ ++ P
Sbjct: 1   MASTTDMST--YKLNHTMIRVKDLDKSLKFYTEVFGMKLIDQWVFEENEFSLSFLAFDGP 58

Query: 385 AEVPRDDDARTKWAMTRKATLELTYNWGTESDD 483
             +    + R+K    R+  LELTYN+GTE  +
Sbjct: 59  GALNHGVE-RSK----REGILELTYNFGTEKKE 86



 Score = 46.4 bits (105), Expect = 4e-06
 Identities = 17/34 (50%), Positives = 25/34 (73%)
 Frame = +3

Query: 498 RFEQLGVKFIKRPNDGKMKGLAFIQDPDGYWIEI 599
           +FE  G+ F K+  DG+MK +AF+ DPD YW+E+
Sbjct: 265 KFEAEGLPFKKKLTDGRMKDIAFLLDPDNYWVEV 298



 Score = 46.0 bits (104), Expect = 5e-06
 Identities = 19/36 (52%), Positives = 25/36 (69%)
 Frame = +3

Query: 504 EQLGVKFIKRPNDGKMKGLAFIQDPDGYWIEIFTSN 611
           E  GV F K+ +DGKMK +AF  DPD YWIE+ + +
Sbjct: 119 ESKGVSFKKKLSDGKMKHIAFALDPDNYWIELVSQS 154


>SPAC1952.16 |rga9||RhoGAp, GTPase activator towards
           Rho/Rac/Cdc42-like small GTPases|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 684

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 9/24 (37%), Positives = 17/24 (70%)
 Frame = +3

Query: 225 HYQGFYVPTNNVQDQRSKEDYTFL 296
           HY G+++P +N  +  +K++Y FL
Sbjct: 414 HYTGYFIPVSN-NELSTKDEYLFL 436


>SPAC732.02c |||fructose-2,6-bisphosphate 2-phosphatase activity
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 408

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 10/30 (33%), Positives = 18/30 (60%)
 Frame = -3

Query: 459 ICQFQRGFPSHGPFSSGVIVSRYFSWILIA 370
           +C    G P+ G  SS + +SRY +W+ ++
Sbjct: 10  LCVCFVGLPASGKTSSAMKLSRYLTWMSVS 39


>SPAC14C4.13 |rad17||RFC related checkpoint protein
           Rad17|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 606

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = +3

Query: 480 RLKLPQRFEQLGVKFIKRPNDGKMKGLAF 566
           +L + Q FE+ G K I+   +GK KG  F
Sbjct: 322 QLSISQSFEKKGTKNIREVKEGKGKGNDF 350


>SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 649

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 15/46 (32%), Positives = 23/46 (50%)
 Frame = +1

Query: 193 EIEALCQTPDPTTKDFMFQQTMYRIKDPRKTIPFYTGVLGMTLLKQ 330
           EI     T DP T + + Q    R+K+   TI F T ++   +LK+
Sbjct: 31  EIILEATTEDPETLENVIQALCERLKEQSWTIVFKTLIVFHVMLKE 76


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,919,914
Number of Sequences: 5004
Number of extensions: 60762
Number of successful extensions: 138
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)