Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt6c19
(704 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 44 2e-05
SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch... 36 0.007
SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 28 1.5
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 28 1.5
SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|c... 27 3.5
SPBC1685.15c |klp6|sot2, SPBC649.01c|kinesin-like protein Klp6|S... 26 4.6
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa... 26 4.6
SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase |Schizosaccharo... 26 4.6
SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ... 26 6.0
SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 26 6.0
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 25 8.0
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 44.0 bits (99), Expect = 2e-05
Identities = 42/148 (28%), Positives = 60/148 (40%)
Frame = +1
Query: 229 EGVVMELADCALPLLAGVLPTANPEEAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFK 408
EG M+L A PL + KD A + +K G R DLL AN+ IFK
Sbjct: 59 EGEAMDLNHAA-PLSHETRVYLGDYKDCKDATAVVITAGKNQKPGETRMDLLKANISIFK 117
Query: 409 EQGQALDKVARKDVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQLAAK 588
E + + K KD +LV NP + K + + T +D R Q +
Sbjct: 118 EILREVTKYT-KDAILLVATNPVDVLTYATLKLTGFPAERVIGSGTIIDTARFQYLIGKL 176
Query: 589 IGVPVKDVKRVIIWGNHSSTQFPDASNA 672
G+ + V II G H ++ S+A
Sbjct: 177 YGLDPQSVNADII-GEHGDSELAVWSHA 203
>SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 509
Score = 35.5 bits (78), Expect = 0.007
Identities = 20/53 (37%), Positives = 26/53 (49%)
Frame = +1
Query: 421 ALDKVARKDVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQL 579
A+ D K +VV + + A +CSKY PSIP +TR Q QS L
Sbjct: 394 AIGASIESDAKAIVVLSTSGNTARLCSKYRPSIP---IVMVTRCPQRARQSHL 443
Score = 25.4 bits (53), Expect = 8.0
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = +1
Query: 424 LDKVARKDVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQLAAKIGV 597
++KV K++KV V N N I SK ++PK + ++++A + K GV
Sbjct: 162 IEKVDDKNLKVRV-----NNNGKISSKKGVNLPKTDVDLPALSEKDKADLRFGVKNGV 214
>SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 855
Score = 27.9 bits (59), Expect = 1.5
Identities = 24/62 (38%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
Frame = -1
Query: 362 PSFLGIAPTRKAAA--TSLKASSGFAVGRTPAKSGRAQSANSMTTPSSTPIIGAISRRWR 189
P F + P RK +A T+L SS P + RA SANS+T+P P A S + R
Sbjct: 516 PQFSAV-PHRKVSAQDTNLMGSSPGMYNHMPYLN-RATSANSITSPGVLPEGMAASLKKR 573
Query: 188 KT 183
T
Sbjct: 574 TT 575
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 27.9 bits (59), Expect = 1.5
Identities = 23/89 (25%), Positives = 37/89 (41%), Gaps = 5/89 (5%)
Frame = -1
Query: 467 PTTRTFTSLRATLSKAWPCSLKMRTLAARRSFLSIPSFLGIAPTRKAA-----ATSLKAS 303
P+ T + L + + P S T A S + S T ++ +T ++
Sbjct: 521 PSYNTSSVLPTSSVSSTPLSSANSTTATSASSTPLTSVNSTTATSASSTPFGNSTITSSA 580
Query: 302 SGFAVGRTPAKSGRAQSANSMTTPSSTPI 216
SG T SG + S+TTP+STP+
Sbjct: 581 SGSTGEFTNTNSGNGDVSGSVTTPTSTPL 609
>SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|chr
1|||Manual
Length = 708
Score = 26.6 bits (56), Expect = 3.5
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -3
Query: 582 SKLGLSPVLIETSHGSEIFFWNRWSILRTY 493
+K LSP+L+ + S I +W+ LR Y
Sbjct: 337 AKHKLSPILLTAMYASSIIYWDADKELRNY 366
>SPBC1685.15c |klp6|sot2, SPBC649.01c|kinesin-like protein
Klp6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 784
Score = 26.2 bits (55), Expect = 4.6
Identities = 22/102 (21%), Positives = 45/102 (44%), Gaps = 2/102 (1%)
Frame = +1
Query: 379 LLAANVRIFKEQGQALDKVARKDVKV--LVVGNPANTNALICSKYAPSIPKENFTAMTRL 552
++ +R ++ + L + D+ V L + N + L+ + PK +
Sbjct: 153 IIFLTMRALLDRVEGLKRTMNVDISVSYLEIYNEKIRDLLVQDPLSMEKPK-SLNICEDA 211
Query: 553 DQNRAQSQLAAKIGVPVKDVKRVIIWGNHSSTQFPDASNAVA 678
+QN + L+ +++V +II GN + T P +NAV+
Sbjct: 212 EQNVSVPGLSYFTPTNLEEVMEIIIRGNSNRTMSPTEANAVS 253
>SPCC18B5.03 |wee1||dual specificity protein kinase
Wee1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 877
Score = 26.2 bits (55), Expect = 4.6
Identities = 23/74 (31%), Positives = 26/74 (35%), Gaps = 9/74 (12%)
Frame = +1
Query: 472 PANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQL---------AAKIGVPVKDVKRVI 624
P N+N S SIP T N AQS L A K+G K +
Sbjct: 240 PPNSNNANPSTLFSSIPSSRHTTSNHFPSNSAQSSLFSPTARPLTARKLGFASSQTKSAV 299
Query: 625 IWGNHSSTQFPDAS 666
NHS DAS
Sbjct: 300 S-NNHSRNSSKDAS 312
>SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 571
Score = 26.2 bits (55), Expect = 4.6
Identities = 8/22 (36%), Positives = 15/22 (68%)
Frame = -1
Query: 521 GIDGAYLEHIRAFVLAGLPTTR 456
G+ G+Y EH+ + G+P+T+
Sbjct: 87 GVAGSYAEHVPVVHIVGMPSTK 108
>SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit
Sec63 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 25.8 bits (54), Expect = 6.0
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -1
Query: 647 VDEWFPQMITLLT 609
VDEWFP+M T LT
Sbjct: 231 VDEWFPKMETSLT 243
>SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 594
Score = 25.8 bits (54), Expect = 6.0
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -1
Query: 521 GIDGAYLEHIRAFVLAGLPTTRTFTS 444
GI GAY E++ +++G P T +S
Sbjct: 89 GIGGAYAENLPVILVSGSPNTNDLSS 114
>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1583
Score = 25.4 bits (53), Expect = 8.0
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = -1
Query: 269 SGRAQSANSMTTPSSTPIIGAISRRWRKTGC*GPKTAPDAI 147
+ R+ S N TPSS+P + SR + PK + D I
Sbjct: 147 TNRSSSDNGFLTPSSSPRSPSCSRVFNAVQLCSPKKSKDDI 187
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,908,529
Number of Sequences: 5004
Number of extensions: 60361
Number of successful extensions: 190
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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