BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt5k06
(566 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 62 6e-11
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po... 52 6e-08
SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces... 29 0.48
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch... 27 1.9
SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces ... 27 1.9
SPBC28F2.09 |||transcription factor TFIIA complex large subunit ... 26 3.4
SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo... 26 3.4
SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c... 26 4.4
SPCC1183.03c |||frataxin homolog|Schizosaccharomyces pombe|chr 3... 25 7.7
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 25 7.7
>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 62.1 bits (144), Expect = 6e-11
Identities = 43/138 (31%), Positives = 71/138 (51%), Gaps = 4/138 (2%)
Frame = +3
Query: 141 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN----ESLRGGDLATQARVWQW 308
N D KFP K+P F DG L+E+ AIA+Y+A+ +L G +A+V Q+
Sbjct: 38 NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLASLNKTRALNGTTAEEKAKVLQY 96
Query: 309 ASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERIT 488
S+++SEL A + P + +++Q + A++ + D L ++T+LV R+T
Sbjct: 97 CSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYLVGSRLT 156
Query: 489 LADVIVFSTLLHAFQHVL 542
LAD+ L +VL
Sbjct: 157 LADIFFTCFLKFGATYVL 174
>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 220
Score = 52.0 bits (119), Expect = 6e-08
Identities = 46/186 (24%), Positives = 84/186 (45%), Gaps = 8/186 (4%)
Frame = +3
Query: 21 MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 200
M G LY++ N R L A+ V + + S D KFP K+P F
Sbjct: 1 MFLGTLYSFKTNTRTVCLLELAKLLDLQVDLVETYPH---KFSADLAAKFPLQKLPVFIG 57
Query: 201 ADGKVLLTESNAIAYYVAN------ESLRGGDLATQARVWQWASWSDSELL-PASCA-WV 356
ADG L + Y+ E L + +A + +W + + +++ P + WV
Sbjct: 58 ADGFELSEVIAIVKYFYEKGKHNDKEGLGPVNEVEEAEMLKWMCFINFDIVTPQNVRPWV 117
Query: 357 FPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQH 536
+ G + + ++ + + + + +LK+ + + RT+LV +R TLAD + F +LL F +
Sbjct: 118 GMFRGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLAD-LFFGSLLRIFFN 176
Query: 537 VLXPER 554
+ E+
Sbjct: 177 SIIDEK 182
>SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 442
Score = 29.1 bits (62), Expect = 0.48
Identities = 19/54 (35%), Positives = 25/54 (46%), Gaps = 4/54 (7%)
Frame = +1
Query: 415 TYWPP*KYWTDIFSHAPSLLPRESHLPMSLSS----VHCCMLSSTCXNPSVRSS 564
TY PP Y D+FS APS+ + S+S V +S +P VR S
Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTESSMSPTPEVVSPAATNSAISSPFVRKS 310
>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
Eme1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 738
Score = 27.1 bits (57), Expect = 1.9
Identities = 16/62 (25%), Positives = 30/62 (48%)
Frame = +3
Query: 48 PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 227
P F++ KA + QY+ +FG +K ++F K+ ++ F S + +L+
Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611
Query: 228 SN 233
SN
Sbjct: 612 SN 613
>SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 267
Score = 27.1 bits (57), Expect = 1.9
Identities = 13/33 (39%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
Frame = +1
Query: 277 IWLPKPVSGSGHHGLTANYCL-LPALGSSLTLV 372
+W+P PV SG T C+ LP G +T V
Sbjct: 23 LWVPTPVGRSGRKHRTLKMCIVLPDTGYDVTQV 55
>SPBC28F2.09 |||transcription factor TFIIA complex large subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 369
Score = 26.2 bits (55), Expect = 3.4
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = -2
Query: 217 STFPSALSNAGTFPAGNFFKKSSDL 143
+TFP A + GTFP G F S L
Sbjct: 52 ATFPWAQAPVGTFPIGQLFDPVSGL 76
>SPBC16A3.11 |eso1||sister chromatid cohesion protein
Eso1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 26.2 bits (55), Expect = 3.4
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +3
Query: 228 SNAIAYYVANESLRGGDLATQARVWQWAS 314
S + A Y+ NE+LR G + +R+W AS
Sbjct: 780 STSSAVYIKNENLRKGFVLGISRIWVSAS 808
>SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 339
Score = 25.8 bits (54), Expect = 4.4
Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 3/39 (7%)
Frame = +1
Query: 307 GHHGLTANYCLLPALGSSLTLV--SCNSTN-RMLNVQSL 414
GH L + C P++GSS L+ +C N R+ V++L
Sbjct: 213 GHTDLIRDICWAPSMGSSYYLIATACKDGNVRIFKVETL 251
>SPCC1183.03c |||frataxin homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 158
Score = 25.0 bits (52), Expect = 7.7
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +1
Query: 268 AEEIWLPKPVSGSGHH 315
A +IWL PVSG H+
Sbjct: 101 AHQIWLSSPVSGPKHY 116
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 25.0 bits (52), Expect = 7.7
Identities = 18/40 (45%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
Frame = +3
Query: 24 AAGVLYTYPENFRAYKALIAAQYSG-TDVKVAPNFV-FGE 137
A G +YT E+FRA K+L S T V+ FV FGE
Sbjct: 329 ALGSVYTIQESFRAEKSLTRRHLSEFTHVEFELPFVNFGE 368
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,521,658
Number of Sequences: 5004
Number of extensions: 53659
Number of successful extensions: 183
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 240047038
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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