Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt5f01
(600 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 198 5e-52
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 87 2e-18
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 81 1e-16
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 56 3e-09
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 55 9e-09
SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe... 31 0.17
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 26 3.7
SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit Prp1|... 26 4.8
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 25 6.4
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 25 6.4
>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 360
Score = 198 bits (483), Expect = 5e-52
Identities = 89/186 (47%), Positives = 123/186 (66%), Gaps = 1/186 (0%)
Frame = +3
Query: 42 MATDNLTALLYKPNDLRLVQTPIPEISED-EVLLRMDCVGICGSDVHYWQKGQCGHFVLE 218
MA +L K D + P +++D +V + + GICGSDVHYW++G G F+L+
Sbjct: 1 MAPAEKAFVLRKKMDTAIEDRPGQTLTDDHQVKVAIKATGICGSDVHYWKEGGIGDFILK 60
Query: 219 EPMIMGHEASGVVAKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLCPDMIFCATP 398
+PMI+GHE++GVV ++G V +L GD VA+EPG CR C++C++GRY+LCP M F ATP
Sbjct: 61 KPMILGHESAGVVVEVGKGVSSLKPGDPVAVEPGCVCRLCDYCRSGRYNLCPHMEFAATP 120
Query: 399 PVHGNLVRYYKHAADFCFKLPDHVTMEEGALLEPLAVGIHACKRGGVSAGHVVLVLGAGP 578
P G L YY DFC KLP +++EEGAL EP++V +HA RG + G VLV+G G
Sbjct: 121 PYDGTLRTYYITTEDFCTKLPKQISVEEGALFEPMSVAVHAMTRGNLKCGSRVLVMGCGT 180
Query: 579 IGLLTM 596
+GLL M
Sbjct: 181 VGLLMM 186
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 87.0 bits (206), Expect = 2e-18
Identities = 62/194 (31%), Positives = 88/194 (45%), Gaps = 24/194 (12%)
Frame = +3
Query: 78 PNDLRLVQTPIPEISED-EVLLRMDCVGIC-GSDVHYWQKGQCGHFVLEEPMIMGHEASG 251
P ++++ + P P I+ +V+++ IC GSD H + G +E+ I+GHE+ G
Sbjct: 45 PLNVKIAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPG---IEKGAILGHESCG 101
Query: 252 VVAKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLCPDMIFCATPPV-----HGNL 416
+VA+ G +V NL +GDRV I + C C FCK Y C V H +
Sbjct: 102 IVAEKGDEVNNLEIGDRVVIAFDLACGQCSFCKRHEYAACDTTNDSKLMDVNYGSHHSAI 161
Query: 417 VRYYKHAADF----------------CFKLPDHVTMEEGALL-EPLAVGIHACKRGGVSA 545
Y K D C KLPD + EG + + L +HAC G V
Sbjct: 162 FGYTKLLGDVPGCQAEYIRVPFAEINCCKLPDDIPDSEGLFMSDVLCTSLHACTLGEVKK 221
Query: 546 GHVVLVLGAGPIGL 587
G V + G GPIGL
Sbjct: 222 GDTVAIWGMGPIGL 235
>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
Adh1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 350
Score = 81.0 bits (191), Expect = 1e-16
Identities = 52/170 (30%), Positives = 83/170 (48%), Gaps = 4/170 (2%)
Frame = +3
Query: 78 PNDLRLVQTPIPEISEDEVLLRMDCVGICGSDVHYWQKGQCGHFVL--EEPMIMGHEASG 251
P +++ + P+ E +DEVL+ + G+C +D+H Q G + L + P+I GHE +G
Sbjct: 18 PENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQ----GDWPLPAKMPLIGGHEGAG 73
Query: 252 VVAKIGSKVKNLTVGDRVAIE-PGVPCRYCEFCKTGRYHLCPDMIFCATPPVHGNLVRYY 428
VV K+G+ V L +GDRV ++ C CE+C +CP I + V G Y
Sbjct: 74 VVVKVGAGVTRLKIGDRVGVKWMNSSCGNCEYCMKAEETICPH-IQLSGYTVDGTFQHYC 132
Query: 429 KHAADFCFKLPDHVTMEEGALLEPLAVGIH-ACKRGGVSAGHVVLVLGAG 575
A +P+ V +E A + + + A K V G + + GAG
Sbjct: 133 IANATHATIIPESVPLEVAAPIMCAGITCYRALKESKVGPGEWICIPGAG 182
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 56.4 bits (130), Expect = 3e-09
Identities = 32/98 (32%), Positives = 53/98 (54%)
Frame = +3
Query: 78 PNDLRLVQTPIPEISEDEVLLRMDCVGICGSDVHYWQKGQCGHFVLEEPMIMGHEASGVV 257
P + VQ P + E V +++ G+C +D Y G+ + P+I+GHE +G+V
Sbjct: 24 PLSIENVQVFPPRVHE--VRIKIVNSGVCHTDA-YTLSGKDPEGLF--PVILGHEGAGIV 78
Query: 258 AKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLC 371
+G +V + VGD V C+ C+FCK+G+ +LC
Sbjct: 79 ESVGPQVTTVQVGDPVIALYTPECKTCKFCKSGKTNLC 116
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 54.8 bits (126), Expect = 9e-09
Identities = 27/81 (33%), Positives = 45/81 (55%)
Frame = +3
Query: 129 EVLLRMDCVGICGSDVHYWQKGQCGHFVLEEPMIMGHEASGVVAKIGSKVKNLTVGDRVA 308
EV +++D +C +D Y G P+++GHE +G+V IG V N+ GD V
Sbjct: 36 EVRVKVDWSAVCHTDA-YTLSGVDPEGAF--PIVLGHEGAGIVESIGEGVINVRPGDHVI 92
Query: 309 IEPGVPCRYCEFCKTGRYHLC 371
+ C+ C+FC++G+ +LC
Sbjct: 93 LLYTPECKECKFCRSGKTNLC 113
>SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 346
Score = 30.7 bits (66), Expect = 0.17
Identities = 33/132 (25%), Positives = 51/132 (38%), Gaps = 4/132 (3%)
Frame = +3
Query: 213 LEEPMIMGHEASGVVAKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLCPDMIFCA 392
L+ P++ G + +G++ K+G V+ GD V Y + T
Sbjct: 58 LQLPVVPGSDGAGIIEKVGEDVEGFEKGDSVVC--NFFTNYLDGTPT---DFATHSALGG 112
Query: 393 TPPVHGNLVRYYKHAADFCFKLPDHVTMEEGALLEPLAV----GIHACKRGGVSAGHVVL 560
T G +Y A P +++ EE A L AV G+ K V G+ VL
Sbjct: 113 TR--DGCFQKYAVLPAHALVHAPKNLSFEEIATLPCAAVTAWNGLFGSKEHQVKPGNNVL 170
Query: 561 VLGAGPIGLLTM 596
VLG G + +
Sbjct: 171 VLGTGGVSTFAL 182
>SPAC27E2.06c |||methionine-tRNA ligase,
mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
Length = 539
Score = 26.2 bits (55), Expect = 3.7
Identities = 19/69 (27%), Positives = 31/69 (44%), Gaps = 1/69 (1%)
Frame = -2
Query: 599 EHGKEAD-GSRSQYQHHVSRRDSAPLARVNAHREGLQ*RSFFHGHVIRKLEAKISCVLVI 423
E GKE S Y +S+ S + N + +Q S FH V+ +L+ IS + +
Sbjct: 173 ETGKEVQWSSEMNYHFLLSKFQSRLIEHYNKNPNFVQ-PSIFHTQVLEELKTGISDLSIS 231
Query: 422 SDEVSVYWG 396
+ + WG
Sbjct: 232 RPKQRLSWG 240
>SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit
Prp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 906
Score = 25.8 bits (54), Expect = 4.8
Identities = 6/32 (18%), Positives = 14/32 (43%)
Frame = +1
Query: 475 WKKERYWSPSRWAFTRARGAESRRDTWCWYWE 570
W +++ W + + D WCW+++
Sbjct: 821 WLEKKADKARSWFLKAVKADQDNGDVWCWFYK 852
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 25.4 bits (53), Expect = 6.4
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -2
Query: 473 GHVIRKLEAKISCVLVISDEVSVYWGRGAED 381
GH ++ +EAK + I + SV G+G D
Sbjct: 211 GHTLKDMEAKSGAKIAIRGKGSVKEGKGRSD 241
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 25.4 bits (53), Expect = 6.4
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +3
Query: 93 LVQTPIPEISEDEVLLRMDC 152
LVQ+ I E SED + L +DC
Sbjct: 1489 LVQSAIRESSEDAIALAVDC 1508
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,529,164
Number of Sequences: 5004
Number of extensions: 51244
Number of successful extensions: 149
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 262236260
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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