BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt5c12
(592 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 83 2e-17
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 59 5e-10
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 59 5e-10
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 59 5e-10
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 49 4e-07
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 35 0.008
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 32 0.054
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 31 0.17
SPBC557.05 |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual 30 0.29
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 29 0.38
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 29 0.67
SPCC736.07c |||cell polarity protein |Schizosaccharomyces pombe|... 29 0.67
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 29 0.67
SPAC11D3.08c |||amino acid permease, unknown 1|Schizosaccharomyc... 29 0.67
SPAC8E11.08c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 28 0.89
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 27 2.7
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 27 2.7
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 25 8.3
SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr 3... 25 8.3
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 83.4 bits (197), Expect = 2e-17
Identities = 37/49 (75%), Positives = 43/49 (87%)
Frame = +3
Query: 444 KEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDXRTLEKYEREAREKSRES 590
KEHVN+VFIGHVDAGKST+GG I+ LTGMVD RT+EK EREA+E +ES
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKES 284
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 58.8 bits (136), Expect = 5e-10
Identities = 26/50 (52%), Positives = 36/50 (72%)
Frame = +3
Query: 441 KKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDXRTLEKYEREAREKSRES 590
+K H+NVV IGHVD+GKST G ++ G +D RT+EK+E+EA E + S
Sbjct: 4 EKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGS 53
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 58.8 bits (136), Expect = 5e-10
Identities = 26/50 (52%), Positives = 36/50 (72%)
Frame = +3
Query: 441 KKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDXRTLEKYEREAREKSRES 590
+K H+NVV IGHVD+GKST G ++ G +D RT+EK+E+EA E + S
Sbjct: 4 EKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGS 53
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 58.8 bits (136), Expect = 5e-10
Identities = 26/50 (52%), Positives = 36/50 (72%)
Frame = +3
Query: 441 KKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDXRTLEKYEREAREKSRES 590
+K H+NVV IGHVD+GKST G ++ G +D RT+EK+E+EA E + S
Sbjct: 4 EKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGS 53
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 49.2 bits (112), Expect = 4e-07
Identities = 27/88 (30%), Positives = 50/88 (56%), Gaps = 6/88 (6%)
Frame = +3
Query: 345 DTQVGNTNPNEDGELTKKIPKKKP------PRVEDTRSKKEHVNVVFIGHVDAGKSTIGG 506
+ ++ N++ +++ KKI K+ P P + + + K V++V GHVD+GKST+ G
Sbjct: 136 EVKMKNSSESDNQPEKKKIKKQNPTDLVSVPEIFEQSNPKPVVHLVVTGHVDSGKSTMLG 195
Query: 507 QIMSLTGMVDXRTLEKYEREAREKSRES 590
+IM G ++ R+++K EA + S
Sbjct: 196 RIMFELGEINSRSMQKLHNEAANSGKGS 223
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 35.1 bits (77), Expect = 0.008
Identities = 19/51 (37%), Positives = 27/51 (52%), Gaps = 3/51 (5%)
Frame = +3
Query: 441 KKEHVNVVFIGHVDAGKSTIGGQI---MSLTGMVDXRTLEKYEREAREKSR 584
KK HVN+ IGHVD GK+T+ I +S G + ++ EK+R
Sbjct: 50 KKPHVNIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKAR 100
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 32.3 bits (70), Expect = 0.054
Identities = 19/51 (37%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
Frame = +3
Query: 372 NEDGELTKKIPKKKPPRVEDTRSKKEHV-NVVFIGHVDAGKSTIGGQIMSL 521
+E GE +KK+ K ++E+ K + + V+ +G D+GKSTI QI L
Sbjct: 8 SESGE-SKKLNSKIEKQIENASKKDKKIYKVLLLGASDSGKSTISKQIKIL 57
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 30.7 bits (66), Expect = 0.17
Identities = 17/44 (38%), Positives = 24/44 (54%), Gaps = 5/44 (11%)
Frame = +3
Query: 456 NVVFIGHVDAGKSTIGGQIMSLTGMVDXRT-----LEKYEREAR 572
N I H+D GKST+ I+ LTG+++ L+K E E R
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINEHNFRNQFLDKLEVERR 103
>SPBC557.05 |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 433
Score = 29.9 bits (64), Expect = 0.29
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +2
Query: 422 SRRYTKQKRTCKCSIYRTCRCRKVDYWWSNNVTHWNG 532
SR+ ++ +TCKC T RC K +N +WNG
Sbjct: 289 SRKNSEVSKTCKCLQKETIRCNK-----KSNCYNWNG 320
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 29.5 bits (63), Expect = 0.38
Identities = 11/49 (22%), Positives = 27/49 (55%), Gaps = 1/49 (2%)
Frame = +3
Query: 399 IPKKKPPRVEDTRSKKEHV-NVVFIGHVDAGKSTIGGQIMSLTGMVDXR 542
+P P ++ + +E++ N + HVD GK+T+ +++ G++ +
Sbjct: 1 MPVIPPEKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSK 49
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 28.7 bits (61), Expect = 0.67
Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +3
Query: 414 PPRVEDTRSKKEHV-NVVFIGHVDAGKSTIGGQIMSLTGMV 533
P V + K +V N+ I HVD GKST+ ++ G++
Sbjct: 6 PEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGII 46
>SPCC736.07c |||cell polarity protein |Schizosaccharomyces pombe|chr
3|||Manual
Length = 699
Score = 28.7 bits (61), Expect = 0.67
Identities = 13/41 (31%), Positives = 24/41 (58%), Gaps = 2/41 (4%)
Frame = +3
Query: 342 LDTQVGNT--NPNEDGELTKKIPKKKPPRVEDTRSKKEHVN 458
L+ + NT NP E+ + T + K PP+ + + + KE++N
Sbjct: 564 LNNRAANTKLNPKEEDKSTVESELKAPPKEKSSETSKEYLN 604
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 28.7 bits (61), Expect = 0.67
Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +3
Query: 414 PPRVEDTRSKKEHV-NVVFIGHVDAGKSTIGGQIMSLTGMV 533
P V + K +V N+ I HVD GKST+ ++ G++
Sbjct: 6 PEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGII 46
>SPAC11D3.08c |||amino acid permease, unknown 1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 550
Score = 28.7 bits (61), Expect = 0.67
Identities = 15/42 (35%), Positives = 20/42 (47%)
Frame = -1
Query: 475 CPINTTFTCSFLLRVSSTRGGFFFGIFFVNSPSSLGFVFPTC 350
C I + + L T GG +F IF + SPSS F+ C
Sbjct: 92 CLIPVSISLGELASSMPTSGGLYFWIFTLASPSSRAFLCWVC 133
>SPAC8E11.08c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
Length = 100
Score = 28.3 bits (60), Expect = 0.89
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = -1
Query: 79 NNLVFFPG*LKLFRFC 32
NNLVFF G LFRFC
Sbjct: 85 NNLVFFMGKCDLFRFC 100
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 26.6 bits (56), Expect = 2.7
Identities = 14/42 (33%), Positives = 25/42 (59%)
Frame = +3
Query: 438 SKKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDXRTLEKYER 563
S++ +N+ IGHV GKST+ + +++G+ R + ER
Sbjct: 19 SRQATINIGTIGHVAHGKSTV---VKAISGVHTVRFKNELER 57
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 26.6 bits (56), Expect = 2.7
Identities = 10/35 (28%), Positives = 20/35 (57%)
Frame = +3
Query: 429 DTRSKKEHVNVVFIGHVDAGKSTIGGQIMSLTGMV 533
D + K+ N+ H+D+GK+T +++ TG +
Sbjct: 52 DKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRI 86
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 25.0 bits (52), Expect = 8.3
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +3
Query: 456 NVVFIGHVDAGKSTIGGQIMSLTG 527
NV I H+DAGK+T+ +++ G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 378
Score = 25.0 bits (52), Expect = 8.3
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = +3
Query: 213 VEEPPPTTASVPPDVSPTADSWEVEADDA 299
VE P PT + P SPT E E+ A
Sbjct: 288 VEHPKPTATTSAPSASPTGVPVEEESHKA 316
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,881,352
Number of Sequences: 5004
Number of extensions: 29441
Number of successful extensions: 121
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 116
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 120
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 256184654
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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