Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt5a02
(703 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 220 2e-58
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 101 1e-22
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 94 2e-20
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 56 4e-09
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 55 1e-08
SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe... 49 6e-07
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 28 1.1
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 3.4
SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit Prp1|... 26 6.0
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 25 7.9
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 25 7.9
>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 360
Score = 220 bits (537), Expect = 2e-58
Identities = 102/216 (47%), Positives = 143/216 (66%), Gaps = 2/216 (0%)
Frame = +2
Query: 50 MATDNLTALLYKPNDLRLVQTPIPEISED-EVLLRMDCVGICGSDVHYWQKGQCGHFVLE 226
MA +L K D + P +++D +V + + GICGSDVHYW++G G F+L+
Sbjct: 1 MAPAEKAFVLRKKMDTAIEDRPGQTLTDDHQVKVAIKATGICGSDVHYWKEGGIGDFILK 60
Query: 227 EPMIMGHEASGVVAKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLCPDMIFCATP 406
+PMI+GHE++GVV ++G V +L GD VA+EPG CR C++C++GRY+LCP M F ATP
Sbjct: 61 KPMILGHESAGVVVEVGKGVSSLKPGDPVAVEPGCVCRLCDYCRSGRYNLCPHMEFAATP 120
Query: 407 PVHGNLVRYYKHAADFCFKLPDHVTMEEGALLEPLAVGIHACKRGGVSAGHVVLVLGAGP 586
P G L YY DFC KLP +++EEGAL EP++V +HA RG + G VLV+G G
Sbjct: 121 PYDGTLRTYYITTEDFCTKLPKQISVEEGALFEPMSVAVHAMTRGNLKCGSRVLVMGCGT 180
Query: 587 IGLLTMLTAKAFGAHKVLIIDILQSRLDFA-KSLGA 691
+GLL M AKA+GA ++ +D SR++FA K +GA
Sbjct: 181 VGLLMMAVAKAYGAIDIVAVDASPSRVEFAQKYVGA 216
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 101 bits (242), Expect = 1e-22
Identities = 70/222 (31%), Positives = 104/222 (46%), Gaps = 24/222 (10%)
Frame = +2
Query: 86 PNDLRLVQTPIPEISED-EVLLRMDCVGIC-GSDVHYWQKGQCGHFVLEEPMIMGHEASG 259
P ++++ + P P I+ +V+++ IC GSD H + G +E+ I+GHE+ G
Sbjct: 45 PLNVKIAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPG---IEKGAILGHESCG 101
Query: 260 VVAKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLCPDMIFCATPPV-----HGNL 424
+VA+ G +V NL +GDRV I + C C FCK Y C V H +
Sbjct: 102 IVAEKGDEVNNLEIGDRVVIAFDLACGQCSFCKRHEYAACDTTNDSKLMDVNYGSHHSAI 161
Query: 425 VRYYKHAADF----------------CFKLPDHVTMEEGALL-EPLAVGIHACKRGGVSA 553
Y K D C KLPD + EG + + L +HAC G V
Sbjct: 162 FGYTKLLGDVPGCQAEYIRVPFAEINCCKLPDDIPDSEGLFMSDVLCTSLHACTLGEVKK 221
Query: 554 GHVVLVLGAGPIGLLTMLTAKAFGAHKVLIIDILQSRLDFAK 679
G V + G GPIGL A+ GA KV+ I+++ R++ A+
Sbjct: 222 GDTVAIWGMGPIGLYAGRWAQILGASKVIGIEVVPERIELAR 263
>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
Adh1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 350
Score = 94.3 bits (224), Expect = 2e-20
Identities = 66/211 (31%), Positives = 104/211 (49%), Gaps = 5/211 (2%)
Frame = +2
Query: 86 PNDLRLVQTPIPEISEDEVLLRMDCVGICGSDVHYWQKGQCGHFVL--EEPMIMGHEASG 259
P +++ + P+ E +DEVL+ + G+C +D+H Q G + L + P+I GHE +G
Sbjct: 18 PENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQ----GDWPLPAKMPLIGGHEGAG 73
Query: 260 VVAKIGSKVKNLTVGDRVAIE-PGVPCRYCEFCKTGRYHLCPDMIFCATPPVHGNLVRYY 436
VV K+G+ V L +GDRV ++ C CE+C +CP I + V G Y
Sbjct: 74 VVVKVGAGVTRLKIGDRVGVKWMNSSCGNCEYCMKAEETICPH-IQLSGYTVDGTFQHYC 132
Query: 437 KHAADFCFKLPDHVTMEEGALLEPLAVGIH-ACKRGGVSAGHVVLVLGA-GPIGLLTMLT 610
A +P+ V +E A + + + A K V G + + GA G +G L +
Sbjct: 133 IANATHATIIPESVPLEVAAPIMCAGITCYRALKESKVGPGEWICIPGAGGGLGHLAVQY 192
Query: 611 AKAFGAHKVLIIDILQSRLDFAKSLGADYTL 703
AKA A +V+ ID + + KS GA+ L
Sbjct: 193 AKAM-AMRVVAIDTGDDKAELVKSFGAEVFL 222
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 56.4 bits (130), Expect = 4e-09
Identities = 32/98 (32%), Positives = 53/98 (54%)
Frame = +2
Query: 86 PNDLRLVQTPIPEISEDEVLLRMDCVGICGSDVHYWQKGQCGHFVLEEPMIMGHEASGVV 265
P + VQ P + E V +++ G+C +D Y G+ + P+I+GHE +G+V
Sbjct: 24 PLSIENVQVFPPRVHE--VRIKIVNSGVCHTDA-YTLSGKDPEGLF--PVILGHEGAGIV 78
Query: 266 AKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLC 379
+G +V + VGD V C+ C+FCK+G+ +LC
Sbjct: 79 ESVGPQVTTVQVGDPVIALYTPECKTCKFCKSGKTNLC 116
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 54.8 bits (126), Expect = 1e-08
Identities = 27/81 (33%), Positives = 45/81 (55%)
Frame = +2
Query: 137 EVLLRMDCVGICGSDVHYWQKGQCGHFVLEEPMIMGHEASGVVAKIGSKVKNLTVGDRVA 316
EV +++D +C +D Y G P+++GHE +G+V IG V N+ GD V
Sbjct: 36 EVRVKVDWSAVCHTDA-YTLSGVDPEGAF--PIVLGHEGAGIVESIGEGVINVRPGDHVI 92
Query: 317 IEPGVPCRYCEFCKTGRYHLC 379
+ C+ C+FC++G+ +LC
Sbjct: 93 LLYTPECKECKFCRSGKTNLC 113
>SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 346
Score = 49.2 bits (112), Expect = 6e-07
Identities = 46/165 (27%), Positives = 70/165 (42%), Gaps = 4/165 (2%)
Frame = +2
Query: 221 LEEPMIMGHEASGVVAKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLCPDMIFCA 400
L+ P++ G + +G++ K+G V+ GD V Y + T
Sbjct: 58 LQLPVVPGSDGAGIIEKVGEDVEGFEKGDSVVCN--FFTNYLDGTPTD---FATHSALGG 112
Query: 401 TPPVHGNLVRYYKHAADFCFKLPDHVTMEEGALLEPLAV----GIHACKRGGVSAGHVVL 568
T G +Y A P +++ EE A L AV G+ K V G+ VL
Sbjct: 113 TRD--GCFQKYAVLPAHALVHAPKNLSFEEIATLPCAAVTAWNGLFGSKEHQVKPGNNVL 170
Query: 569 VLGAGPIGLLTMLTAKAFGAHKVLIIDILQSRLDFAKSLGADYTL 703
VLG G + + A A GA+ V + +L+FAK LGA +T+
Sbjct: 171 VLGTGGVSTFALQFALAAGAN-VTVTSSSDEKLEFAKKLGATHTI 214
>SPAC27E2.06c |||methionine-tRNA ligase,
mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
Length = 539
Score = 28.3 bits (60), Expect = 1.1
Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 1/74 (1%)
Frame = -1
Query: 622 KGFSCEHGKEAD-GSRSQYQHHVSRRDSAPLARVNAHREGLQ*RSFFHGHVIRKLEAKIS 446
K S E GKE S Y +S+ S + N + +Q S FH V+ +L+ IS
Sbjct: 168 KRVSMETGKEVQWSSEMNYHFLLSKFQSRLIEHYNKNPNFVQ-PSIFHTQVLEELKTGIS 226
Query: 445 CVLVISDEVSVYWG 404
+ + + + WG
Sbjct: 227 DLSISRPKQRLSWG 240
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 26.6 bits (56), Expect = 3.4
Identities = 15/39 (38%), Positives = 17/39 (43%), Gaps = 2/39 (5%)
Frame = -2
Query: 648 SIMSTLCAPKALAVSMVRRPMGPAPSTSTTCPAET--PP 538
S ST P + S P PSTST+C T PP
Sbjct: 309 STSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPP 347
>SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit
Prp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 906
Score = 25.8 bits (54), Expect = 6.0
Identities = 6/32 (18%), Positives = 14/32 (43%)
Frame = +3
Query: 483 WKKERYWSPSRWAFTRARGAESRRDTWCWYWE 578
W +++ W + + D WCW+++
Sbjct: 821 WLEKKADKARSWFLKAVKADQDNGDVWCWFYK 852
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 25.4 bits (53), Expect = 7.9
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -1
Query: 481 GHVIRKLEAKISCVLVISDEVSVYWGRGAED 389
GH ++ +EAK + I + SV G+G D
Sbjct: 211 GHTLKDMEAKSGAKIAIRGKGSVKEGKGRSD 241
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 25.4 bits (53), Expect = 7.9
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +2
Query: 101 LVQTPIPEISEDEVLLRMDC 160
LVQ+ I E SED + L +DC
Sbjct: 1489 LVQSAIRESSEDAIALAVDC 1508
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,985,499
Number of Sequences: 5004
Number of extensions: 61153
Number of successful extensions: 198
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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