Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt4l21
         (738 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1952.17c ||SPAC890.01c|GTPase activating protein|Schizosacch...   117   2e-27
SPBC530.01 |gyp1||GTPase activating protein Gyp1 |Schizosaccharo...    62   6e-11
SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pom...    27   2.1  
SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi...    27   2.8  
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch...    27   2.8  
SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharom...    27   2.8  
SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16 |Schizosa...    26   4.9  
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig...    26   6.4  
SPAC4G9.04c |||cleavage and polyadenylation specificity factor |...    25   8.5  

>SPAC1952.17c ||SPAC890.01c|GTPase activating
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 619

 Score =  117 bits (281), Expect = 2e-27
 Identities = 61/197 (30%), Positives = 106/197 (53%), Gaps = 11/197 (5%)
 Frame = +2

Query: 95  HKARIKAFEEVLD-QDVIDMQQLQKLAFNGIPDDKGLRSLVWKILLHYLPQEKNARETTL 271
           +K RI+ F+++L+ ++ I +  L  L   GIPD+  LR+  W ++L +LP +++  ++ L
Sbjct: 3   YKQRIEKFKDILNSEEPISLPGLCSLCIQGIPDEYSLRAKAWMLMLEFLPTDRSNWQSVL 62

Query: 272 IKKRQLYKQFIEEIIVSP----------GGPTDHPLNMSPDSSWSTYFKDNEVLLQIDKD 421
            K R+ Y  F++E+++ P          G  +DHPLN S DS W  YF DN++L QIDKD
Sbjct: 63  EKHRKTYTSFVQELLIDPWRKLTLHEESGENSDHPLNTSDDSKWKEYFDDNQILEQIDKD 122

Query: 422 VRRLCPDISFFQSATEFPCPEVVNSNGIKRLHKRVEQSVLKYATLERRGLGVAKLSNEIK 601
           +RR  PD+SFFQ  +E      VN N  + +    E   ++    +     +  +    +
Sbjct: 123 IRRTLPDLSFFQGKSEINKKPSVN-NVSENISVNTEDDKVEEVGQKLNYTKITSIDQSQE 181

Query: 602 RAENITSGDYAPLNEGC 652
              ++++ D++   E C
Sbjct: 182 TPVHLSTIDFSKFQEEC 198



 Score = 45.6 bits (103), Expect = 7e-06
 Identities = 19/24 (79%), Positives = 21/24 (87%)
 Frame = +3

Query: 666 EVVERMLFLYAKLNPGQGYVQGMN 737
           E  ER+LF+YAKLNPG GYVQGMN
Sbjct: 250 EAAERILFIYAKLNPGIGYVQGMN 273


>SPBC530.01 |gyp1||GTPase activating protein Gyp1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 514

 Score = 62.5 bits (145), Expect = 6e-11
 Identities = 30/64 (46%), Positives = 41/64 (64%)
 Frame = +2

Query: 101 ARIKAFEEVLDQDVIDMQQLQKLAFNGIPDDKGLRSLVWKILLHYLPQEKNARETTLIKK 280
           +RIK F  +LD  ++D+  L+ LA+NGIP +   R +VWK LL YLP   + RE TL +K
Sbjct: 190 SRIKKFSRILDAPIVDLNALRTLAWNGIPSEH--RPIVWKYLLGYLPCNASRREVTLKRK 247

Query: 281 RQLY 292
           R  Y
Sbjct: 248 RDEY 251



 Score = 32.3 bits (70), Expect = 0.074
 Identities = 12/27 (44%), Positives = 21/27 (77%)
 Frame = +3

Query: 657 LTGEVVERMLFLYAKLNPGQGYVQGMN 737
           LT  ++ER+L+++A  +P  GYVQG++
Sbjct: 294 LTQRMLERILYVWASRHPASGYVQGIS 320


>SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 772

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 11/22 (50%), Positives = 15/22 (68%)
 Frame = +3

Query: 672 VERMLFLYAKLNPGQGYVQGMN 737
           + R+L  Y++ NP  GY QGMN
Sbjct: 571 LRRILVAYSRHNPHIGYCQGMN 592


>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
           Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score = 27.1 bits (57), Expect = 2.8
 Identities = 15/31 (48%), Positives = 19/31 (61%)
 Frame = +2

Query: 503 IKRLHKRVEQSVLKYATLERRGLGVAKLSNE 595
           IK  HK+VE +VLKY  +E  G  V +L  E
Sbjct: 91  IKHKHKKVELAVLKYYKVEDDG-SVKRLRRE 120


>SPAC589.10c |||ribomal-ubiquitin fusion protein
           Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score = 27.1 bits (57), Expect = 2.8
 Identities = 15/31 (48%), Positives = 19/31 (61%)
 Frame = +2

Query: 503 IKRLHKRVEQSVLKYATLERRGLGVAKLSNE 595
           IK  HK+VE +VLKY  +E  G  V +L  E
Sbjct: 91  IKHKHKKVELAVLKYYKVEDDG-SVKRLRRE 120


>SPBC2F12.13 |klp5|sot1|kinesin-like protein
           Klp5|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 883

 Score = 27.1 bits (57), Expect = 2.8
 Identities = 17/65 (26%), Positives = 31/65 (47%), Gaps = 1/65 (1%)
 Frame = +2

Query: 176 NGIPDDKGLRSLVWKILLHYLPQEKNARETTL-IKKRQLYKQFIEEIIVSPGGPTDHPLN 352
           +G   D GL  L  K L   +   ++ +   L +   ++Y + I +++VSP      PLN
Sbjct: 155 SGTMQDPGLIYLTLKELFERMDHLRDEKIFDLRLSYLEIYNETIRDLLVSPTPNQAKPLN 214

Query: 353 MSPDS 367
           +  D+
Sbjct: 215 LREDA 219


>SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 759

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 13/31 (41%), Positives = 18/31 (58%)
 Frame = +2

Query: 347 LNMSPDSSWSTYFKDNEVLLQIDKDVRRLCP 439
           +NM+PD      F DN + L+   DVR +CP
Sbjct: 466 MNMTPD------FNDNLLQLKFTDDVRNVCP 490


>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
           E3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1647

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 13/37 (35%), Positives = 23/37 (62%)
 Frame = +2

Query: 200 LRSLVWKILLHYLPQEKNARETTLIKKRQLYKQFIEE 310
           L+SL  + L +Y  +E+N   +TL++ +QL K  + E
Sbjct: 837 LKSLAKEFLSNY--KEENLENSTLVQLKQLSKHLLSE 871


>SPAC4G9.04c |||cleavage and polyadenylation specificity factor
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 638

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 13/30 (43%), Positives = 16/30 (53%)
 Frame = -1

Query: 597 ISLLSLATPRPLLSNVAYLRTDCSTRLCNR 508
           +S  SLATPRP LS++ Y         C R
Sbjct: 399 LSKSSLATPRPKLSSLLYENYSNQCANCGR 428


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,845,827
Number of Sequences: 5004
Number of extensions: 55279
Number of successful extensions: 195
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 193
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)