Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt4d23
(705 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC13G1.02 |||mannose-1-phosphate guanyltransferase |Schizosacc... 204 1e-53
SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase Mpg1|Sc... 108 7e-25
SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon... 46 5e-06
SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchang... 32 0.070
SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha... 26 4.6
SPAC2E12.02 |hsf1|hstf, hsf|transcription factor Hsf1|Schizosacc... 26 4.6
SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch... 26 6.0
SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar ... 25 8.0
SPAC3A11.06 |mvp1||sorting nexin Mvp1|Schizosaccharomyces pombe|... 25 8.0
>SPBC13G1.02 |||mannose-1-phosphate guanyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 414
Score = 204 bits (498), Expect = 1e-53
Identities = 89/198 (44%), Positives = 137/198 (69%), Gaps = 3/198 (1%)
Frame = +1
Query: 121 AVILIGGPQKGTRFRPLSLDIPKPLFPIAGLPLIQHHIAACTKLGECKEILIIGSYTTTQ 300
AVIL+GGP +GTRFRPLS D+PKPLF I G +I HH+AA +K+ K++ ++G Y +
Sbjct: 5 AVILVGGPSRGTRFRPLSFDVPKPLFKIGGREMIYHHLAALSKIESVKDVFLVGFYDESV 64
Query: 301 MTQFVNDMQKLYRVI--IRYLQEFTPLGTGGGLYHFRDQIRAGNPSAFFLLNGDVCADFP 474
F+N++ + I+YL+E+ LGTGGGLYHFRDQI G+ S F+++ DVC FP
Sbjct: 65 FKDFINEVASHFPSFNRIKYLREYNCLGTGGGLYHFRDQILKGHTSNVFVMHADVCCSFP 124
Query: 475 LREMYEFHEEKPNAIVTIMGTEATRQQSVHYGCMVRNGSNA-VTHYVEKPNSYVSTLINC 651
L+E+ H EK A+VT+M T+ +++ + ++GC+V S V HYV+KP+SY+S +I+C
Sbjct: 125 LQELLNVHHEK-KALVTLMATKVSKEDASNFGCLVEEPSTGRVLHYVDKPSSYLSNIISC 183
Query: 652 GVYVCSLNVFQVMAEAFQ 705
G+Y+ ++F + +A++
Sbjct: 184 GIYIFDASIFDEIKKAYE 201
>SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase
Mpg1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 363
Score = 108 bits (260), Expect = 7e-25
Identities = 69/190 (36%), Positives = 104/190 (54%), Gaps = 2/190 (1%)
Frame = +1
Query: 115 LKAVILIGGPQKGTRFRPLSLDIPKPLFPIAGLPLIQHHIAACTKLGECKEILIIGSYTT 294
+KA+IL+GG GTR RPL+L +PKPL P+I H + A G +I++ +Y
Sbjct: 1 MKALILVGG--FGTRLRPLTLTLPKPLVEFGNKPMILHQVEALAAAG-VTDIVLAVNYRP 57
Query: 295 TQMTQFVNDMQKLYRVIIRYLQEFTPLGTGGGLYHFRDQIRAGNPSAFFLLNGDVCADFP 474
M + + +K Y V I + E PLGT G L RD I A + S FF+LN DV ++P
Sbjct: 58 EIMVEALKKYEKEYNVNITFSVENEPLGTAGPLALARD-ILAKDHSPFFVLNSDVICEYP 116
Query: 475 LREMYEFHEEKPNAIVTIMGTEATRQQSVHYGCMVR--NGSNAVTHYVEKPNSYVSTLIN 648
++ FH+ A TI+ T+ ++ YG +V N + + +VEKP +VS IN
Sbjct: 117 FADLAAFHKAH-GAEGTIVVTKV--EEPSKYGVVVHYPNSESLIERFVEKPVEFVSNRIN 173
Query: 649 CGVYVCSLNV 678
G+Y+ + +V
Sbjct: 174 GGIYILNPSV 183
>SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 678
Score = 46.0 bits (104), Expect = 5e-06
Identities = 40/151 (26%), Positives = 70/151 (46%), Gaps = 7/151 (4%)
Frame = +1
Query: 115 LKAVILIGGPQKGTRFRPLSLDIPKPLFPIAGLPLIQHHIAACTKLGECKEILIIGSYTT 294
L+A++L RFRPL+LD P+ L P+A PLI++ G +E+ +
Sbjct: 18 LQAIVL--SDSYNYRFRPLTLDKPRCLLPLANTPLIEYTFEFLALAG-VQEVYVFCCAHA 74
Query: 295 TQMTQFVNDMQ---KLYRVIIRYLQEFTPLGTGGGLYHFRDQIRAGNPSAFFLLNGDVCA 465
Q+ +++ + + + L G L + S F L++GDV +
Sbjct: 75 GQIREYIEKSKWNLPSSPFSVNTIVSRESLSVGDALRELDSKQLI--TSDFILVSGDVVS 132
Query: 466 DFPLREMYEFH----EEKPNAIVTIMGTEAT 546
+ PL E+ + H E+ NAI+T++ EA+
Sbjct: 133 NVPLNEVLKEHRKRREDDKNAIMTMVVREAS 163
>SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchange
factor Sec73 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 32.3 bits (70), Expect = 0.070
Identities = 21/61 (34%), Positives = 32/61 (52%)
Frame = -2
Query: 548 RVASVPIIVTIALGFSS*NSYISLNGKSAQTSPFNRKNALGFPARI*SLKWYRPPPVPSG 369
R+AS+P++ + FS + YI KS QTS N + + SL+ YR P +P+
Sbjct: 226 RIASLPVVKRPPIQFSISSDYIKPVDKSDQTS-----NVPSPSSSVGSLRLYRSPSIPND 280
Query: 368 V 366
V
Sbjct: 281 V 281
>SPAC2G11.11c |prh1||ATP-dependent RNA helicase
Prh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 719
Score = 26.2 bits (55), Expect = 4.6
Identities = 12/40 (30%), Positives = 25/40 (62%), Gaps = 3/40 (7%)
Frame = +1
Query: 211 LPLIQHHIAACTKLGECKEILII---GSYTTTQMTQFVND 321
LP+ + H C ++ + + I+++ GS +TQ+ QF+N+
Sbjct: 96 LPIWEAHDTLCQQIQDNRVIVVVGETGSGKSTQIPQFLNE 135
>SPAC2E12.02 |hsf1|hstf, hsf|transcription factor
Hsf1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 609
Score = 26.2 bits (55), Expect = 4.6
Identities = 15/42 (35%), Positives = 18/42 (42%)
Frame = +1
Query: 106 INMLKAVILIGGPQKGTRFRPLSLDIPKPLFPIAGLPLIQHH 231
INML I G + PL D P L+P+ QHH
Sbjct: 379 INMLDDANSIEGSNMNS-LSPLLFDYPNSLYPVNNTSSEQHH 419
>SPBC13G1.10c |mug81||ATP-dependent RNA helicase
Slh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1935
Score = 25.8 bits (54), Expect = 6.0
Identities = 8/13 (61%), Positives = 12/13 (92%)
Frame = +3
Query: 69 ITLTYFYRRIHRH 107
+T TYFYRR+H++
Sbjct: 1561 LTCTYFYRRVHQN 1573
>SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar
membrane transporter Hmt1|Schizosaccharomyces pombe|chr
3|||Manual
Length = 830
Score = 25.4 bits (53), Expect = 8.0
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = -2
Query: 527 IVTIALGFSS*NSYISLNGKSAQTSPFNRKNALG 426
+V + +GF S S L + A + P+ RKN G
Sbjct: 15 LVLLYVGFFSIGSLNLLQKRKATSDPYRRKNRFG 48
>SPAC3A11.06 |mvp1||sorting nexin Mvp1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 664
Score = 25.4 bits (53), Expect = 8.0
Identities = 15/38 (39%), Positives = 17/38 (44%)
Frame = -2
Query: 188 FGMSSDRGRNRVPFCGPPISITAFNMLMAMYSSIKISK 75
F SSD P PP S TA N+ + SI SK
Sbjct: 131 FNRSSDNETPLFPNNSPPASTTALNLSSNIVPSINESK 168
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,055,868
Number of Sequences: 5004
Number of extensions: 67028
Number of successful extensions: 162
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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