Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt3f14
(740 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar... 263 2e-71
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi... 135 7e-33
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 30 0.40
SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces ... 30 0.40
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 28 1.2
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 28 1.6
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 28 1.6
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 27 2.1
SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces pom... 27 2.1
SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 4.9
SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pom... 26 6.5
SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|ch... 25 8.6
SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|c... 25 8.6
>SPBC354.02c |sec61||translocon alpha subunit
Sec61|Schizosaccharomyces pombe|chr 2|||Manual
Length = 479
Score = 263 bits (645), Expect = 2e-71
Identities = 120/192 (62%), Positives = 153/192 (79%), Gaps = 1/192 (0%)
Frame = +2
Query: 167 IRFLEVIKPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADPF 346
+RFL+++KPF LPEIA PERK+ F++K+LWT +TL IFLV Q+PL+GI+SSDS+DP
Sbjct: 4 LRFLDLVKPFAPFLPEIAAPERKVPFKQKMLWTGVTLLIFLVMSQVPLYGIVSSDSSDPL 63
Query: 347 YWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAQKLFGM 523
W+R+ILA+NRGTLMELGISPIVTS +++QLL G+++IEV + DR ++ QK +
Sbjct: 64 LWLRMILAANRGTLMELGISPIVTSSMLVQLLVGSQLIEVNMELKSDREMYQLVQKFLAI 123
Query: 524 VITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLFVAGLIVLLLDELLQKGYGLGSGISL 703
+I GQA YV+TGMYG P ++GAG+CLL+I+QL A LIVLLLDELLQKGYGLGSGISL
Sbjct: 124 IIAFGQATAYVLTGMYGRPQDLGAGICLLLILQLAAASLIVLLLDELLQKGYGLGSGISL 183
Query: 704 FIATNICETXVW 739
FIAT CE W
Sbjct: 184 FIATINCENIFW 195
>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 475
Score = 135 bits (326), Expect = 7e-33
Identities = 67/193 (34%), Positives = 115/193 (59%), Gaps = 1/193 (0%)
Frame = +2
Query: 164 GIRFLEVIKPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADP 343
G RF+ IKP S+LPE+ P+ ++ EK+ W A + ++ + IP++G +D+ DP
Sbjct: 3 GARFINFIKPLSSLLPEVEGPKTHLELVEKLGWMAGCVVVYQILSIIPVYGAEKTDTLDP 62
Query: 344 FYWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAQKLFG 520
RV+ S+ LM G++PI S ++Q+LA K I V + DR LF AQK+
Sbjct: 63 INNFRVLDGSSASGLMITGLAPIYLSSFLLQILASKKKIAVNFNLIIDRVLFQNAQKVVS 122
Query: 521 MVITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLFVAGLIVLLLDELLQKGYGLGSGIS 700
++ + A+ YV +G YG S++G +++I+Q+F+ G++ + L E+++KG+GLGSG
Sbjct: 123 ALLYLILAVTYVSSGYYGSFSDLGIFRFIMLILQIFLPGIVCIYLCEIIEKGHGLGSGPV 182
Query: 701 LFIATNICETXVW 739
L + ++I +W
Sbjct: 183 LLLGSHILGNIMW 195
>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 780
Score = 29.9 bits (64), Expect = 0.40
Identities = 14/45 (31%), Positives = 26/45 (57%)
Frame = -3
Query: 717 FVAINREIPEPNPYPFWSNSSSNNTIRPATNSCMIMRRHTPAPIS 583
+ +I+RE+ N PF SNS S ++ T++ +R + +PI+
Sbjct: 29 YTSISRELDYANQSPFSSNSFSPTELKARTSATFDVRSASTSPIN 73
>SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 346
Score = 29.9 bits (64), Expect = 0.40
Identities = 23/67 (34%), Positives = 30/67 (44%), Gaps = 4/67 (5%)
Frame = -3
Query: 513 NFCAPLNKALSFGVSPTS-MILAPASNCMISPDVTIGEIPSSINV---PLFDARITRIQ* 346
+ CA +NKAL G+ TS A C PD I + S V PL + RI ++
Sbjct: 104 SLCAAINKALDAGIPITSPAFYADEKQC---PDTLIASVFDSATVEKIPLLEERIRIMRA 160
Query: 345 KGSALSD 325
G L D
Sbjct: 161 SGRVLVD 167
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 28.3 bits (60), Expect = 1.2
Identities = 19/54 (35%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +2
Query: 167 IRFLEVI-KPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMS 325
IRFL+ KP+ S + R++ + K WT + I L+ PLFGI S
Sbjct: 1055 IRFLDPPPKPYLSYKMYLNDFTRQLHYIPKGSWTVQIVAIVLLILLPPLFGIFS 1108
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 27.9 bits (59), Expect = 1.6
Identities = 17/66 (25%), Positives = 31/66 (46%), Gaps = 3/66 (4%)
Frame = -2
Query: 703 QRDTRTQSISFLEQ---FIEQQHNKTGHKQLYDNEEAHSGTNFTGFTIHSSHYINNSLTN 533
Q+ +S S ++ F E Q++ TG+K + A +G + G + +S +
Sbjct: 1700 QKSPSVESFSIIDNDNPFHEGQNSSTGYKDIVQGLLAENGVSEAGVDVMTSIVSSTIPIV 1759
Query: 532 SNHHTK 515
SNH T+
Sbjct: 1760 SNHQTE 1765
>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 676
Score = 27.9 bits (59), Expect = 1.6
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = -3
Query: 705 NREIPEPNPYPFWSNSSSNNT 643
N +P P P P +NSS+NNT
Sbjct: 395 NSNMPPPAPRPVTNNSSNNNT 415
>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1334
Score = 27.5 bits (58), Expect = 2.1
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = -2
Query: 646 HNKTGHKQLYDNEEAHSGTNFTGFTIHSSHYINNSLTNSNH 524
+NK HK+L DNE SGT + T+ + + N T H
Sbjct: 515 NNKNHHKELEDNE---SGTRISPSTLGDTSFPNGIFTLLTH 552
>SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 336
Score = 27.5 bits (58), Expect = 2.1
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
Frame = -2
Query: 640 KTGHKQLYDNEEAHSGTNFTGFTIHSSHYINN-SLTNSNHHT 518
+T +Y + E H+G G +HSS NN S T+SN T
Sbjct: 144 QTHGDDVYLSTEEHNGNAARGLPVHSSFKQNNSSQTSSNKGT 185
>SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 340
Score = 26.2 bits (55), Expect = 4.9
Identities = 17/61 (27%), Positives = 29/61 (47%)
Frame = -3
Query: 393 SINVPLFDARITRIQ*KGSALSDDIIPNKGIWQHTKKINNVIAVHNTFSRN*ILRSGFAI 214
S+ + LFD I+ + S +SD IP K +++ N + T+S N + +S
Sbjct: 273 SLLLGLFDKSISMLNCHSSKISDGDIPYKSHQVFLQELQNKYSEIKTYSSNKLNKSSIKS 332
Query: 213 S 211
S
Sbjct: 333 S 333
>SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 772
Score = 25.8 bits (54), Expect = 6.5
Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 1/53 (1%)
Frame = -3
Query: 729 VSQMFVAINREIPEPNPYPFWS-NSSSNNTIRPATNSCMIMRRHTPAPISLGS 574
VS FV+ E PE Y + NSSSN +R + + + P+SL S
Sbjct: 288 VSSTFVSATSE-PEVETYSVSTKNSSSNKNLRSSLSKLLSTSNLNNKPLSLSS 339
>SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 845
Score = 25.4 bits (53), Expect = 8.6
Identities = 15/56 (26%), Positives = 28/56 (50%)
Frame = -3
Query: 516 NNFCAPLNKALSFGVSPTSMILAPASNCMISPDVTIGEIPSSINVPLFDARITRIQ 349
N C +K + P ++I +P+S S ++ + PSS+NV + R+ +Q
Sbjct: 368 NGLCRNDSKCRFLMILPETIIKSPSSFIGDSYNIANIDPPSSMNVQSSEFRVGEVQ 423
>SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1315
Score = 25.4 bits (53), Expect = 8.6
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -2
Query: 211 WQYTAERLNYFQKSYSHFDL 152
WQY A+R Y+Q S +DL
Sbjct: 1001 WQYYAKREQYYQASIVLYDL 1020
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,166,492
Number of Sequences: 5004
Number of extensions: 68092
Number of successful extensions: 208
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 351258950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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