BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt30o22
(675 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC20F10.02c |||DUF1741 family protein|Schizosaccharomyces pomb... 30 0.27
SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium tra... 28 1.4
SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|... 27 3.3
SPAC2G11.13 |atg22||autophagy associated protein Atg22 |Schizosa... 27 3.3
SPBC27.02c |ask1|mug181|DASH complex subunit Ask1|Schizosaccharo... 26 4.3
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 26 5.7
SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyce... 25 7.6
>SPBC20F10.02c |||DUF1741 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 600
Score = 30.3 bits (65), Expect = 0.27
Identities = 19/76 (25%), Positives = 36/76 (47%), Gaps = 3/76 (3%)
Frame = +2
Query: 257 KVVXIYESFFRGEDLTTDNPTFWDEFFLLKPKIPQLEAEIHKLSTEQL-INMKE--SMNI 427
K+V Y+ N FW EFFLL+P++ L + + ++L +N + SM +
Sbjct: 12 KIVFYYKCLLNKNWNEPINNIFWGEFFLLQPRLEVLSQLLRECPKQELTVNGPKFHSMYL 71
Query: 428 LVEQCIEMLRQDHHIR 475
+ + ++ + IR
Sbjct: 72 YISEILKSKAESLRIR 87
>SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium
transporting Cta4 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1211
Score = 27.9 bits (59), Expect = 1.4
Identities = 15/36 (41%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Frame = -3
Query: 448 FYTLFNQYIHTFFHVYQLFCGQFVYFCF-QLWYFWL 344
F TLF ++ F V+Q+FC V +C WYF L
Sbjct: 189 FGTLFKEHAVAPFFVFQIFC--CVLWCLDDYWYFSL 222
>SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 574
Score = 26.6 bits (56), Expect = 3.3
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = -3
Query: 448 FYTLFNQYIHTFFHVYQLFCGQFVYFCFQLWYFWLQKKEFIPKSRVIS 305
FY+L ++ TFFH Q C + W+F+ K K+ ++S
Sbjct: 341 FYSLHFLWLCTFFHALQ--CA-IISSIVSQWFFYRDTKSSATKTNLVS 385
>SPAC2G11.13 |atg22||autophagy associated protein Atg22
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 529
Score = 26.6 bits (56), Expect = 3.3
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = -1
Query: 435 STNIFILSFMFISCSVDSLCISASSCGIFGFRRKNSS 325
S +F+ S +FI+C + + S SS IFG R N S
Sbjct: 328 SIRLFLFSRLFINCGIQT---SLSSAVIFGKARLNLS 361
>SPBC27.02c |ask1|mug181|DASH complex subunit
Ask1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 307
Score = 26.2 bits (55), Expect = 4.3
Identities = 13/49 (26%), Positives = 26/49 (53%), Gaps = 2/49 (4%)
Frame = +2
Query: 197 NFTMAMRKRSGSGSKRH--LKEKVVXIYESFFRGEDLTTDNPTFWDEFF 337
+ T+A+ + + SK H + K++ I E + + + D+ FW +FF
Sbjct: 13 SITLALYEIDANFSKCHRTVTTKILPIVEKYAKNCNTIWDSSKFWKQFF 61
>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 971
Score = 25.8 bits (54), Expect = 5.7
Identities = 12/47 (25%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = -3
Query: 445 YTLFNQYIHTFFHVYQLFCGQF-VYFCFQLWYFWLQKKEFIPKSRVI 308
Y+ + I +F+ +++ G F VY + L + W+ EF+P ++
Sbjct: 572 YSFASIRISSFYSPAKVWGGAFLVYILYSLAHVWVVAYEFVPGGPIL 618
>SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 747
Score = 25.4 bits (53), Expect = 7.6
Identities = 14/52 (26%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = -3
Query: 406 VYQLFCGQ-FVYFCFQLWYFWLQKKEFIPKSRVISR*VLSSKERFINXYNLL 254
V L C Q FV+ F L + KE + ++++ + + + K+ + YN+L
Sbjct: 57 VTSLRCLQLFVHLTFLLGVYTQLPKEMLSQAKIKALPIYTPKKNLVQIYNIL 108
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,457,209
Number of Sequences: 5004
Number of extensions: 47565
Number of successful extensions: 142
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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