Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt30n22
(720 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces... 51 1e-07
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 46 4e-06
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 46 5e-06
SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz... 45 1e-05
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 42 1e-04
SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi... 41 2e-04
SPBP8B7.29 |||para-aminobenzoate synthase |Schizosaccharomyces p... 31 0.17
SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces po... 31 0.22
SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch... 27 2.0
SPCC330.11 |btb1||BTB/POZ domain protein Btb1|Schizosaccharomyce... 27 2.0
SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchang... 27 3.6
SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces ... 26 4.7
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 6.2
SPCC320.14 ||SPCC330.15c|threo-3-hydroxyaspartate ammonia-lyase ... 26 6.2
SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.2
>SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 662
Score = 51.2 bits (117), Expect = 1e-07
Identities = 50/185 (27%), Positives = 80/185 (43%), Gaps = 18/185 (9%)
Frame = +3
Query: 186 DAVALI----NGETGHKTTFKEILQETVNAASGMKKIGVKRGDVIAFCGENINEFFIGIL 353
DAVALI G T++E+L A ++ +GV GD +A I E I +L
Sbjct: 101 DAVALIYEADEPNQGRYITYRELLASVSQCAGALQSMGVGMGDRVAIYMPMIPETIIAML 160
Query: 354 STICCGATVTTLNVMYSEDEINHVLNISKPSMIF--------GSEIAFKRNLG-VFKSLP 506
+ + GA + + +S + + +N S+ +I G I K + P
Sbjct: 161 AIVRLGAIHSVIFAGFSAESVADRVNDSECKVIITADESHRGGKRIPLKGVVNKALTECP 220
Query: 507 FIKNFV--QLNGSPVE---RGIQTFSSLLLPTDPNMYEPALEARGDDTAFILYSSGTTGL 671
IK + Q + P G + ++P P PA+ + F+LY+SG+TG
Sbjct: 221 TIKKVLVFQRSAEPTASMVEGRDVWWHDIIPKFPRYCPPAV-VNPEHPLFLLYTSGSTGK 279
Query: 672 PKGVM 686
PKGV+
Sbjct: 280 PKGVV 284
>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 512
Score = 46.4 bits (105), Expect = 4e-06
Identities = 41/175 (23%), Positives = 71/175 (40%), Gaps = 1/175 (0%)
Frame = +3
Query: 180 EEDAVALINGETGHKTTFKEILQETVNAASGMKKIGVKRGDVIAFCGENINEFFIGILST 359
+ A AL+ + +F E+ ++ + +G+K GD + N EF + +
Sbjct: 13 DASARALVAPSLNAELSFSELRIAIMDLQRQIASLGIKVGDPVNIAIPNGLEFVVAFYAV 72
Query: 360 ICCGATVTTLNVMYSEDEINHVLNISKPSMIFGSEIAFKRNLGVFKSLPFIKNFV-QLNG 536
A LN Y + E ++ K ++ E + N ++ + V +L
Sbjct: 73 SWQRAICGPLNSNYKQSEFEFYIDDLKSKLVIVPEGSVAANTPAVRAAKKLSVAVAELAW 132
Query: 537 SPVERGIQTFSSLLLPTDPNMYEPALEARGDDTAFILYSSGTTGLPKGVMLTHLN 701
P R ++ N +P + DD +L++SGTTG PK V LTH N
Sbjct: 133 CPKSRLVRIVH--FEGAKINAPQPLGLPQPDDVMLVLHTSGTTGRPKVVPLTHKN 185
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 46.0 bits (104), Expect = 5e-06
Identities = 41/162 (25%), Positives = 74/162 (45%)
Frame = +3
Query: 222 KTTFKEILQETVNAASGMKKIGVKRGDVIAFCGENINEFFIGILSTICCGATVTTLNVMY 401
+ T+ E+ + A +K G + G +IA + E FI +L+ + G L+V
Sbjct: 2751 RLTYSELNERANKLAHNLKSYGFRVGSIIAVYFDKCIEAFISMLAILKAGCCFLALDVSA 2810
Query: 402 SEDEINHVLNISKPSMIFGSEIAFKRNLGVFKSLPFIKNFVQLNGSPVERGIQTFSSLLL 581
+ I +++ S ++ + + + L S+ N L+ S + G +S+ +
Sbjct: 2811 PTERIRYIVTDSTAVLVMSTGELYTKLLNA--SI----NVTILDAS--DPG--NYSNNI- 2859
Query: 582 PTDPNMYEPALEARGDDTAFILYSSGTTGLPKGVMLTHLNAL 707
N Y E + A++LY+SG+TG PKG LTH N +
Sbjct: 2860 ---ENPYTKDFE--DSNLAYVLYTSGSTGKPKGCCLTHHNVV 2896
Score = 35.9 bits (79), Expect = 0.006
Identities = 13/23 (56%), Positives = 19/23 (82%)
Frame = +3
Query: 627 DDTAFILYSSGTTGLPKGVMLTH 695
D A++LY+SG+TG PKGV ++H
Sbjct: 359 DSVAYVLYTSGSTGNPKGVAISH 381
Score = 25.8 bits (54), Expect = 6.2
Identities = 22/103 (21%), Positives = 48/103 (46%), Gaps = 6/103 (5%)
Frame = +3
Query: 84 KNNVVYGGPVLKVDAHLSFGQY--IIDRLQDNSQEE-DAVAL---INGETGHKTTFKEIL 245
K+N++ P H +F II + + E ++AL N + + ++K
Sbjct: 1728 KHNLISRSPSSPPPIHTNFDTNADIITLFEKEANEHPSSIALHFVYNVDKENIYSYKFFS 1787
Query: 246 QETVNAASGMKKIGVKRGDVIAFCGENINEFFIGILSTICCGA 374
+ ++ A+ + IG+K+ DV+A + +++ +L + GA
Sbjct: 1788 EYSIKASYWLHSIGIKKNDVVAVFIDKSLDYYSLMLGVLRIGA 1830
>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 676
Score = 45.2 bits (102), Expect = 1e-05
Identities = 36/166 (21%), Positives = 71/166 (42%), Gaps = 8/166 (4%)
Frame = +3
Query: 228 TFKEILQETVNAASGMKKIGVKRGDVIAFCGENINEFFIGILSTICCGATVTTLNVMYSE 407
+F +I + + A ++K+G+ +GD + + + + T+ T E
Sbjct: 98 SFNDIYDKALRYAGALRKLGLNKGDKFELYAPTSAFWLLTAEACLSQSMTIVTAYDTLGE 157
Query: 408 DEINHVLNISKPSMIFGSEIAFKRNLGVFKSLPFIKNFVQLNGSPVE-----RGIQTFSS 572
+ + H L S ++ K + K + ++ + N + E + I+
Sbjct: 158 EGLLHSLRESGVRGMYTEGHLLKTLVNPLKEIESLEVIIYRNDAKEEDIKTIQEIRPNLK 217
Query: 573 LLLPTDPNMYEPALEA---RGDDTAFILYSSGTTGLPKGVMLTHLN 701
L+ D P +E ++ I+Y+SG+TGLPKGV+L+H N
Sbjct: 218 LIKFADFEKMSPPVEPDPPSPEEICCIMYTSGSTGLPKGVILSHKN 263
>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 41.5 bits (93), Expect = 1e-04
Identities = 39/171 (22%), Positives = 65/171 (38%), Gaps = 11/171 (6%)
Frame = +3
Query: 228 TFKEILQETVNAASGMKKIGVKRGDVIAFCGENINEFFIGILSTICCGATVTTLNVMYSE 407
+F ++ + + SG+ G+ + F ++F + T E
Sbjct: 102 SFNKVYEIALALGSGLVASGITSETTMLFFAATSAKWFTTAQGCSSQAIPIVTAYETLGE 161
Query: 408 DEINHVLNISKPSMIFGSEIAFKRNLGVFKSLPFIKNFVQLNGSPVERGIQTFSSL---- 575
D I L+ K IF + LG K ++K V + +P E ++ S
Sbjct: 162 DGIYTSLDECKSRAIFTDPNLIPKLLGPLKQSTWVKLIV-CSSTPSEDLVELVKSTAPDV 220
Query: 576 -------LLPTDPNMYEPALEARGDDTAFILYSSGTTGLPKGVMLTHLNAL 707
LL +P + DD +Y+SG+TG PKGV+L H N +
Sbjct: 221 EIITYDNLLSLGKEKPQPPHPPKADDICCYMYTSGSTGKPKGVVLLHRNII 271
>SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1419
Score = 40.7 bits (91), Expect = 2e-04
Identities = 37/173 (21%), Positives = 74/173 (42%), Gaps = 12/173 (6%)
Frame = +3
Query: 228 TFKEILQETVNAASGMKKIGVKRGDVIAFCGENINEFFIGILSTICCGATVTTLNVMYSE 407
T+++I + + A + K G++RGDV+ + + ++ + GAT + ++ Y
Sbjct: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
Query: 408 DEINHVLNISKPSMIFGSEIA----------FKRNLGVFKSLPFIK--NFVQLNGSPVER 551
L+++KP + E A +++L + +P +K L G V +
Sbjct: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
Query: 552 GIQTFSSLLLPTDPNMYEPALEARGDDTAFILYSSGTTGLPKGVMLTHLNALY 710
G +L + + D T + ++SG+ G+PKGV H + Y
Sbjct: 434 GADDILQHVLHLKSE--QTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAY 484
>SPBP8B7.29 |||para-aminobenzoate synthase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 718
Score = 31.1 bits (67), Expect = 0.17
Identities = 17/64 (26%), Positives = 31/64 (48%)
Frame = +3
Query: 378 VTTLNVMYSEDEINHVLNISKPSMIFGSEIAFKRNLGVFKSLPFIKNFVQLNGSPVERGI 557
+ +L ++ DE N V++I + ++G KSL KNF+ L +P + +
Sbjct: 146 IDSLQILAQSDEDNIVMSIKATKFPHFGILYHPESVGSSKSLKIFKNFLSLADTPNIQCV 205
Query: 558 QTFS 569
+FS
Sbjct: 206 NSFS 209
>SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1564
Score = 30.7 bits (66), Expect = 0.22
Identities = 19/60 (31%), Positives = 31/60 (51%), Gaps = 1/60 (1%)
Frame = -3
Query: 688 SITPLG-RPVVPEEYRMNAVSSPRASSAGSYILGSVGSNNELKVWIPRSTGLPFNCTKFL 512
S +P G +V + + + SP +++ + LG GS+ + K+WI L FN KFL
Sbjct: 1428 SSSPAGAEQIVMNGFFYSIMESPLSTALSTGGLGLDGSSIQYKIWIRGILPLLFNIVKFL 1487
>SPBC13G1.10c |mug81||ATP-dependent RNA helicase
Slh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1935
Score = 27.5 bits (58), Expect = 2.0
Identities = 25/73 (34%), Positives = 36/73 (49%)
Frame = +3
Query: 162 LQDNSQEEDAVALINGETGHKTTFKEILQETVNAASGMKKIGVKRGDVIAFCGENINEFF 341
L DNSQ E +L + K+ KE L+E + G+ G+ R D E + F
Sbjct: 556 LLDNSQHEK-YSLAQRDVS-KSKNKE-LKELFKYSMGIHNAGMLRSD--RHLTERL--FS 608
Query: 342 IGILSTICCGATV 380
+GIL +CC AT+
Sbjct: 609 MGILKILCCTATL 621
>SPCC330.11 |btb1||BTB/POZ domain protein Btb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1347
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/49 (26%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Frame = -3
Query: 355 LKIPIKNSFIFSPQNAMTSPRFTPIFFIPD---AALTVSWSISLNVVLC 218
+K+ + S + TS +FTP++ AAL ++W+ +++LC
Sbjct: 435 MKLLLDRDSTSSKNSTRTSFKFTPLWIFESSDLAALDIAWTADNSLILC 483
>SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchange
factor Sec73 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 26.6 bits (56), Expect = 3.6
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +2
Query: 641 HSIFFRHNWSA*RSNANT 694
HS +F HNWS+ AN+
Sbjct: 89 HSFYFNHNWSSDLQQANS 106
>SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 2493
Score = 26.2 bits (55), Expect = 4.7
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = -3
Query: 322 SPQNAMTSPRFTPIFFIPDAALTVSWSISLNVVLCP 215
SP + + P T +F PD +L ++ +N CP
Sbjct: 700 SPPSGLLGPTDTAVFVKPDLSLEKLPTLEINTFNCP 735
>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1158
Score = 25.8 bits (54), Expect = 6.2
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = -3
Query: 343 IKNSFIFSPQNAMTSPRFTPIFFIPDAALTVSWSISLN 230
I+N+F+++ + A TS F + T+S S+ +N
Sbjct: 305 IENNFLYTKRRANTSDSFDSVLSDSSTTPTISSSVQVN 342
>SPCC320.14 ||SPCC330.15c|threo-3-hydroxyaspartate ammonia-lyase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 323
Score = 25.8 bits (54), Expect = 6.2
Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 4/54 (7%)
Frame = +3
Query: 423 VLNISKPSMIFGSEIAFK----RNLGVFKSLPFIKNFVQLNGSPVERGIQTFSS 572
VL S + F +E+ FK + +G FK + QLN + + G+ TFSS
Sbjct: 29 VLTSSTVNKEFVAEVFFKCENFQKMGAFKFRGALNALSQLNEAQRKAGVLTFSS 82
>SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 821
Score = 25.4 bits (53), Expect = 8.2
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = +3
Query: 564 FSSLLLPTDPNMYEPALEARGDDTAFILYSSGTTGLP 674
F LLL + PN Y P L + D ++ S +T P
Sbjct: 216 FHHLLLKSYPNAYVPRLPPKDDHDTYLNSSEDSTLSP 252
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,997,783
Number of Sequences: 5004
Number of extensions: 65217
Number of successful extensions: 219
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 209
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 218
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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