Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt30f06
         (725 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|...    61   1e-10
SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|...    27   2.7  
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz...    27   2.7  
SPAC15E1.04 |||thymidylate synthase |Schizosaccharomyces pombe|c...    26   6.3  
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni...    25   8.3  
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo...    25   8.3  
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate...    25   8.3  

>SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 532

 Score = 61.3 bits (142), Expect = 1e-10
 Identities = 47/133 (35%), Positives = 67/133 (50%), Gaps = 4/133 (3%)
 Frame = +2

Query: 188 KNVILFVGDGMGPNTVTATRIY----KGGESHRLVYEKFPHVGLLKTYSANKMVPDSSCS 355
           K VI+ V DGMGP +++ TR +       E +RL  ++   +G  +T S++ ++ DS+  
Sbjct: 60  KFVIMMVSDGMGPGSLSMTRSFVETLNDKEGYRLPLDEHL-IGSSRTRSSSSLITDSAAG 118

Query: 356 ATALFCGVKANQETIGVDASVKHKDCSASLRPEARLKSLASIALKAGKSAGFVTTMRVTH 535
           ATA  C  K     +GV  +   K C   L  EA        A +AG   G V T RVT 
Sbjct: 119 ATAFSCANKTYNGAVGVLDN--EKPCGTIL--EA--------AKEAGYLTGIVVTSRVTD 166

Query: 536 ATPGPIYAHSADR 574
           ATP    AH+A+R
Sbjct: 167 ATPASFSAHAANR 179


>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
           Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
 Frame = -1

Query: 704 IQEPVCLHL--GLPASLFRASLPQVASRYPCKQQR*QAFCLRIPIYDQHCGRK 552
           IQ+   LHL   L   +   SL  +AS+Y C++Q  +    R+P    +C +K
Sbjct: 61  IQKESTLHLVLRLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRKK 113


>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
           Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
 Frame = -1

Query: 704 IQEPVCLHL--GLPASLFRASLPQVASRYPCKQQR*QAFCLRIPIYDQHCGRK 552
           IQ+   LHL   L   +   SL  +AS+Y C++Q  +    R+P    +C +K
Sbjct: 61  IQKESTLHLVLRLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRKK 113


>SPAC15E1.04 |||thymidylate synthase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 625

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 13/27 (48%), Positives = 14/27 (51%)
 Frame = +1

Query: 508 FCDNYASHPCDPGSHLRPQC*S*MGMR 588
           FC  Y S PC PG   +PQ  S M  R
Sbjct: 500 FCQFYVSEPCKPGG--KPQLSSMMYQR 524


>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
            Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2199

 Score = 25.4 bits (53), Expect = 8.3
 Identities = 12/24 (50%), Positives = 15/24 (62%)
 Frame = -3

Query: 657  PGQSSTSCLAISLQAAALAGILSS 586
            PG  S +C+ ISL   AL  IL+S
Sbjct: 1688 PGAYSNACVDISLSNLALCSILNS 1711


>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1336

 Score = 25.4 bits (53), Expect = 8.3
 Identities = 14/38 (36%), Positives = 19/38 (50%)
 Frame = +2

Query: 254  KGGESHRLVYEKFPHVGLLKTYSANKMVPDSSCSATAL 367
            K G  H+  Y KFP VG ++    +   PDS  +  AL
Sbjct: 1081 KPGNLHKSGYLKFPLVGKIEFDGVSFAYPDSERNHLAL 1118


>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
           transferase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 453

 Score = 25.4 bits (53), Expect = 8.3
 Identities = 10/36 (27%), Positives = 21/36 (58%)
 Frame = +2

Query: 449 PEARLKSLASIALKAGKSAGFVTTMRVTHATPGPIY 556
           PE +L+   S+ L + +++  ++   +TH  P PI+
Sbjct: 262 PEEQLEQKISLLLSSTRNSPTMSNSSLTHLLPTPIF 297


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,167,605
Number of Sequences: 5004
Number of extensions: 64332
Number of successful extensions: 150
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)