Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt30c01
         (723 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||M...   110   2e-25
SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha sub...    40   5e-04
SPBC24C6.09c |||phosphoketolase |Schizosaccharomyces pombe|chr 2...    38   0.002
SPBC725.04 |||oxalyl-CoA decarboxylase |Schizosaccharomyces pomb...    27   2.7  
SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-...    26   6.3  

>SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 685

 Score =  110 bits (264), Expect = 2e-25
 Identities = 73/215 (33%), Positives = 109/215 (50%), Gaps = 14/215 (6%)
 Frame = +3

Query: 120 NKLRIDSIVATNASKSGHPTSCASMAEIMSVLFFHTMRYKISAPRDASADRFILSKGHAA 299
           N +R  ++  T  +KSGHP +   +A    VLF   M++  + P+  + DRFILS GHA 
Sbjct: 15  NTIRTLAVDTTAHAKSGHPGAPMGLAPAAHVLFSRIMKFNPAHPKWLNRDRFILSNGHAC 74

Query: 300 PILYAAWAEAGL-FPLDELKNLRKLDSDLEGHP---TPRLNFVDVGTGSLGQGLAVAAGM 467
            + Y      G    +++LK  R++ S   GHP    P LN ++ G G LGQG+A A G+
Sbjct: 75  VLQYIMCHLLGYKLTIEDLKQFRQVGSKTPGHPETHNPDLN-IETGAGPLGQGIASAVGL 133

Query: 468 AYVGKYFDQAPYR----------VYCLVGDGEAAEGSIWESLHFASHYKLDNLVVIFDVN 617
           A +GK    A Y            +C +GDG   EG   E+   A H KL NL+ ++D N
Sbjct: 134 A-IGKAHSAAVYNKPGFDLFSNYTFCFLGDGCLQEGVSSEACSLAGHLKLSNLIAVWDNN 192

Query: 618 RLGQSEPTSLQHQLEVYDARLKAFGLNSLVVDGHD 722
           ++     TS+    +V + R +A+G N + V   D
Sbjct: 193 KITIDGATSMSFDEDV-EKRFEAYGWNIVRVANGD 226


>SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha
           subunit Pda1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 409

 Score = 39.5 bits (88), Expect = 5e-04
 Identities = 27/100 (27%), Positives = 47/100 (47%)
 Frame = +3

Query: 423 GTGSLGQGLAVAAGMAYVGKYFDQAPYRVYCLVGDGEAAEGSIWESLHFASHYKLDNLVV 602
           G G +G  + + AG+ +  KY ++ P   + L GDG + +G  +E+ + A   KL  L V
Sbjct: 180 GNGIVGAQIPLGAGIGFAQKYLEK-PTTTFALYGDGASNQGQAFEAFNMA---KLWGLPV 235

Query: 603 IFDVNRLGQSEPTSLQHQLEVYDARLKAFGLNSLVVDGHD 722
           IF          TS +    + +   +   +  L+V+G D
Sbjct: 236 IFACENNKYGMGTSAERSSAMTEFYKRGQYIPGLLVNGMD 275


>SPBC24C6.09c |||phosphoketolase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 825

 Score = 37.5 bits (83), Expect = 0.002
 Identities = 40/135 (29%), Positives = 58/135 (42%), Gaps = 12/135 (8%)
 Frame = +3

Query: 273 FILSKGHAAP-ILYAAWAEAGLFPL--------DELKNLRKLDSDLEGHPTPRLNFVDVG 425
           F++  GH AP IL A + E  L P         + L NL    S   G P+  +N    G
Sbjct: 125 FVVGPGHGAPAILSALFLEDSLGPFYPRYQFTKEGLNNLINTFSLPGGFPS-HVNAEVPG 183

Query: 426 TGSLGQGLAVAAGMAYVGKYFDQAPYRVYCLVGDGEAAEG---SIWESLHFASHYKLDNL 596
               G  L  A  ++Y G   D+    V C+VGDGEA  G   + W +  F    +   +
Sbjct: 184 AIHEGGELGYALSVSY-GAVLDRPDLIVTCVVGDGEAETGPTATSWHAHKFLDPAESGAV 242

Query: 597 VVIFDVNRLGQSEPT 641
           + + ++N    SE T
Sbjct: 243 IPVLELNGYKISERT 257


>SPBC725.04 |||oxalyl-CoA decarboxylase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 574

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 26/100 (26%), Positives = 45/100 (45%)
 Frame = +3

Query: 318 WAEAGLFPLDELKNLRKLDSDLEGHPTPRLNFVDVGTGSLGQGLAVAAGMAYVGKYFDQA 497
           W   G   +D  + L ++      HP  RL+   + T  +G G A+A+  A+     D+ 
Sbjct: 390 WVSEGANTMDRGRQLLEVT-----HPRGRLDAGTMSTMGVGMGYAIASAFAHSS---DK- 440

Query: 498 PYRVYCLVGDGEAAEGSIWESLHFASHYKLDNLVVIFDVN 617
                 +V +G++A G     L  A   +LD LV++ + N
Sbjct: 441 -----IVVVEGDSAFGFSAMELETAIRNQLDLLVIVINNN 475


>SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+,
           L-lysine forming] |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 368

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 14/52 (26%), Positives = 22/52 (42%)
 Frame = -2

Query: 632 GLPQTVNIEDDDQVVQLVVAGKVQRLPDAALGCLSVAHQAIHPVGRLVEVLP 477
           GL   +    DD   ++   G       +A+ CL  AHQ +HP  +   + P
Sbjct: 116 GLLYDLEFLQDDNGRRVAAFGYHAGFAGSAISCLVWAHQLLHPNKQFPAIRP 167


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,697,784
Number of Sequences: 5004
Number of extensions: 51874
Number of successful extensions: 144
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 339215786
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)