Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt2m15
         (721 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.12 |atp11||F1-ATPase chaperone Atp11 |Schizosaccharomyc...    53   5e-08
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe...    28   1.2  
SPBC20F10.04c |nse4|rad62|Smc5-6 complex non-SMC subunit Nse4|Sc...    27   3.6  
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc...    26   4.7  
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo...    26   4.7  
SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo...    25   8.2  

>SPAC3A12.12 |atp11||F1-ATPase chaperone Atp11 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 286

 Score = 52.8 bits (121), Expect = 5e-08
 Identities = 37/140 (26%), Positives = 66/140 (47%), Gaps = 8/140 (5%)
 Frame = +2

Query: 314 KQALGKEINVPDKK-LEDIFKIELVIDKSSTEIKSIWEEYHQNKEVISATIPVDVYSNIK 490
           K  +G+     D   L     +E + +  ++ I+ +W   +   +++SA IP ++Y  + 
Sbjct: 91  KSLVGQNAKKSDLSGLNRYIDVEKIKELPTSTIEKLWRARNIGDDILSACIPKEIYEKML 150

Query: 491 ETMNHFPVFLFPLPR-SQGYEFIMCQM---YGHTVHF--TPLLAYQVHKENAPECLTMVH 652
                +P F+ PLPR  +G E    Q      +  H   T LL Y++    A     M+H
Sbjct: 151 SRARMYPYFVLPLPRGDKGIESHFLQWNFPNKNEAHLLVTSLLEYKLKGSYAAPHTIMLH 210

Query: 653 YTELSD-KGIILMRGEYDKK 709
           + +L + KGI LMR +++ K
Sbjct: 211 FADLLNLKGITLMRCQFEPK 230


>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 973

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 15/33 (45%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
 Frame = +2

Query: 353 KLEDIFKIELV-IDKSSTEIKSIWEEYHQNKEV 448
           +L  I K+ LV +DK  TEI+ ++E Y  NK V
Sbjct: 711 ELRGIDKLALVQLDKFQTEIRELYESYSINKVV 743


>SPBC20F10.04c |nse4|rad62|Smc5-6 complex non-SMC subunit
           Nse4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 300

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 12/41 (29%), Positives = 24/41 (58%)
 Frame = +2

Query: 362 DIFKIELVIDKSSTEIKSIWEEYHQNKEVISATIPVDVYSN 484
           +I  I+LV+D +    ++I +EY+  + +I    PV+  +N
Sbjct: 254 EIRNIQLVLDMTMDLYENIIKEYNIKESIIPTRAPVETSTN 294


>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 732

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 16/74 (21%), Positives = 37/74 (50%)
 Frame = +3

Query: 234 VTRLRKMKQKRNLHLLILGNSHQSLIQNKHSEKKLMCQTRNWRTYLK*NLLLTRVQQKLK 413
           V R+ K+  K +  L  + N   +L++NK   ++L+   + W+      +L  +   ++ 
Sbjct: 538 VARITKLVAKPHEQLSGVFNQIANLLENKEKSRELLEAWQEWK------ILNAKDDTRIG 591

Query: 414 VYGKSIIKIKRSYR 455
               +++++KRS R
Sbjct: 592 TTNSNLLRLKRSNR 605


>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1811

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 3/48 (6%)
 Frame = -1

Query: 205  WRCIL*-LCYSVSI--FLIVGISFQILKCLLHSHRSCSKTASSYITVV 71
            WR I   L Y+  I   L++  +  ++  L H H SC  T  +YI ++
Sbjct: 1303 WRTIFHILAYASKIENLLVLQCAISVVSSLGHEHISCVLTQGAYIDLI 1350


>SPBC12C2.02c |ste20|ste16|sterility protein
           Ste20|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1309

 Score = 25.4 bits (53), Expect = 8.2
 Identities = 7/19 (36%), Positives = 14/19 (73%)
 Frame = +2

Query: 155 YYEKYANRIAELQNTSPEE 211
           +Y+ ++NR + +QNT P +
Sbjct: 754 FYKPFSNRFSSIQNTKPNQ 772


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,000,702
Number of Sequences: 5004
Number of extensions: 64393
Number of successful extensions: 217
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 206
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 217
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)