Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt2k08
         (764 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch...    44   3e-05
SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo...    32   0.078
SPAC12G12.11c |||DUF544 family protein|Schizosaccharomyces pombe...    32   0.10 
SPBC16C6.05 |||translation initiation factor |Schizosaccharomyce...    29   0.96 
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac...    25   9.0  
SPAC1142.01 ||SPAC17G6.18|DUF654 family protein|Schizosaccharomy...    25   9.0  

>SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 538

 Score = 43.6 bits (98), Expect = 3e-05
 Identities = 20/37 (54%), Positives = 28/37 (75%)
 Frame = +1

Query: 196 DLNDQITKQGDLVRSLKAAKAEKTKVDEAVKVLLELK 306
           D+N++IT QG+LVRSLK+  A K  +D+ V  LL+LK
Sbjct: 7   DINERITSQGNLVRSLKSQGASKEDIDKEVAKLLQLK 43



 Score = 32.3 bits (70), Expect = 0.078
 Identities = 14/26 (53%), Positives = 20/26 (76%)
 Frame = +1

Query: 403 LDQQITKQGDLVRSLKTSKAEKSKID 480
           ++++IT QG+LVRSLK+  A K  ID
Sbjct: 8   INERITSQGNLVRSLKSQGASKEDID 33



 Score = 31.9 bits (69), Expect = 0.10
 Identities = 14/35 (40%), Positives = 24/35 (68%)
 Frame = +1

Query: 586 SNDVTALDRDVTAQGDLVRSLKASKADKAKIDEAV 690
           ++ +  ++  +T+QG+LVRSLK+  A K  ID+ V
Sbjct: 2   ADSLVDINERITSQGNLVRSLKSQGASKEDIDKEV 36


>SPBC12C2.02c |ste20|ste16|sterility protein
           Ste20|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1309

 Score = 32.3 bits (70), Expect = 0.078
 Identities = 14/53 (26%), Positives = 28/53 (52%)
 Frame = +1

Query: 160 KPVAAALTPTSSDLNDQITKQGDLVRSLKAAKAEKTKVDEAVKVLLELKAKYK 318
           KPV    T T+ D++      GD ++ +    +++ K+ E +K  LE++ K +
Sbjct: 2   KPVRRGQTDTALDISSHAKTNGDFIKKMNTTDSKRLKLLEDLKGKLEVECKIR 54


>SPAC12G12.11c |||DUF544 family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 365

 Score = 31.9 bits (69), Expect = 0.10
 Identities = 14/36 (38%), Positives = 19/36 (52%)
 Frame = -3

Query: 291 HFDSFVNFCLFGFSSFERSHQISLFCDLVVQI*RGW 184
           HF   +N CL+    FE  H+I  FC   V++  GW
Sbjct: 119 HFGQQLNPCLYDIEKFEYGHEI--FCTFGVRLVHGW 152


>SPBC16C6.05 |||translation initiation factor |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 190

 Score = 28.7 bits (61), Expect = 0.96
 Identities = 20/67 (29%), Positives = 33/67 (49%), Gaps = 2/67 (2%)
 Frame = +1

Query: 124 ITPKPAQSVQASKPVAAALTPTSSDLNDQITKQGDLVRSLKAAKAEKTK--VDEAVKVLL 297
           I  K A  + A+K    A    ++D  D+I  QGDL   +     EK K   ++ +K++ 
Sbjct: 123 IETKKAAKMLANKFATGASVTKTADKKDEIVVQGDLNYDIFDFILEKFKEVPEDNIKIVE 182

Query: 298 ELKAKYK 318
           + K+K K
Sbjct: 183 DTKSKKK 189


>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
           Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 167

 Score = 25.4 bits (53), Expect = 9.0
 Identities = 16/41 (39%), Positives = 24/41 (58%), Gaps = 5/41 (12%)
 Frame = +2

Query: 527 PP--DKIGSQDPRLQQWPPPHRPMT---SPLSIVTSPRRET 634
           PP  DK G +D   ++W P H P T   S +S+++SP  E+
Sbjct: 96  PPGDDKYGYEDAG-ERWLPVHSPETILISVISMLSSPNDES 135


>SPAC1142.01 ||SPAC17G6.18|DUF654 family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 667

 Score = 25.4 bits (53), Expect = 9.0
 Identities = 13/41 (31%), Positives = 23/41 (56%)
 Frame = +1

Query: 196 DLNDQITKQGDLVRSLKAAKAEKTKVDEAVKVLLELKAKYK 318
           DL++Q +   + +  L+++K     +DE  K   ELK KY+
Sbjct: 116 DLDNQ-SSDNEKLEGLESSKNIDDDIDEIEKAAAELKLKYR 155


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.315    0.129    0.372 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,503,505
Number of Sequences: 5004
Number of extensions: 41499
Number of successful extensions: 134
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 367316502
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)