Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt2c23
(765 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI0000D56C3B Cluster: PREDICTED: similar to Probable N... 279 4e-74
UniRef50_Q9VK89 Cluster: Probable N(2),N(2)-dimethylguanosine tR... 273 3e-72
UniRef50_UPI00015B5CEF Cluster: PREDICTED: similar to ENSANGP000... 268 1e-70
UniRef50_A7RIG7 Cluster: Predicted protein; n=2; Nematostella ve... 249 7e-65
UniRef50_UPI0000E48B85 Cluster: PREDICTED: hypothetical protein;... 235 9e-61
UniRef50_Q23270 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 233 5e-60
UniRef50_Q9NXH9-2 Cluster: Isoform 2 of Q9NXH9 ; n=2; Homo/Pan/G... 225 7e-58
UniRef50_Q9NXH9 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 225 7e-58
UniRef50_A6QP09 Cluster: LOC539613 protein; n=2; Bos taurus|Rep:... 220 3e-56
UniRef50_Q54PG3 Cluster: Putative uncharacterized protein; n=1; ... 212 9e-54
UniRef50_P15565 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 209 5e-53
UniRef50_Q6C410 Cluster: Similar to sp|P15565 Saccharomyces cere... 202 6e-51
UniRef50_Q339G6 Cluster: N-dimethylguanosine tRNA methyltransfer... 192 8e-48
UniRef50_Q5C276 Cluster: SJCHGC07232 protein; n=1; Schistosoma j... 183 4e-45
UniRef50_UPI00004990C0 Cluster: N2,N2-dimethylguanosine tRNA met... 179 8e-44
UniRef50_Q9P804 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 176 4e-43
UniRef50_Q9SRU7 Cluster: Probable N(2),N(2)-dimethylguanosine tR... 175 8e-43
UniRef50_A1CC48 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 163 4e-39
UniRef50_Q5A863 Cluster: Likely N2,N2-dimethylguanosine-specific... 161 2e-38
UniRef50_Q4WEX8 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 159 9e-38
UniRef50_A6RDC7 Cluster: Putative uncharacterized protein; n=1; ... 159 9e-38
UniRef50_Q8RU67 Cluster: Putative tRNA (Guanine-N2-)-Methyltrans... 156 5e-37
UniRef50_Q1DMI8 Cluster: Putative uncharacterized protein; n=1; ... 155 1e-36
UniRef50_Q0UJY7 Cluster: Putative uncharacterized protein; n=1; ... 150 4e-35
UniRef50_Q7S9X0 Cluster: Putative uncharacterized protein NCU063... 149 1e-34
UniRef50_UPI000023D9FF Cluster: hypothetical protein FG09705.1; ... 142 6e-33
UniRef50_A6RZH6 Cluster: Putative uncharacterized protein; n=2; ... 138 1e-31
UniRef50_Q22HL7 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 133 4e-30
UniRef50_A2FDH4 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 132 7e-30
UniRef50_Q4P6Q4 Cluster: Putative uncharacterized protein; n=1; ... 125 1e-27
UniRef50_A0CLP3 Cluster: Chromosome undetermined scaffold_20, wh... 122 1e-26
UniRef50_A1RX58 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;... 121 2e-26
UniRef50_Q2NI56 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 119 7e-26
UniRef50_Q9V1P3 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 111 4e-25
UniRef50_Q8ZWT5 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 109 2e-24
UniRef50_A5UM08 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 111 2e-23
UniRef50_Q386C5 Cluster: N(2), N(2)-dimethylguanosine tRNA methy... 110 4e-23
UniRef50_Q5KQ20 Cluster: TRNA (Guanine-N2-)-methyltransferase, p... 108 1e-22
UniRef50_Q58356 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 107 3e-22
UniRef50_Q97ZH0 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 92 7e-21
UniRef50_O29443 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 95 7e-21
UniRef50_O67010 Cluster: Probable N(2),N(2)-dimethylguanosine tR... 101 3e-20
UniRef50_Q0W1J5 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 85 5e-20
UniRef50_Q7QW00 Cluster: GLP_239_44022_45620; n=1; Giardia lambl... 99 6e-20
UniRef50_Q4QG60 Cluster: N(2), N(2)-dimethylguanosine tRNA methy... 100 8e-20
UniRef50_A7DP78 Cluster: tRNA (Guanine-N(2)-)-methyltransferase;... 99 1e-19
UniRef50_A4YHH9 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;... 91 2e-19
UniRef50_UPI00015BCD4C Cluster: UPI00015BCD4C related cluster; n... 97 4e-19
UniRef50_A4RKM5 Cluster: Putative uncharacterized protein; n=1; ... 97 6e-19
UniRef50_Q8PU28 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 97 6e-19
UniRef50_A3HAS8 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;... 91 1e-18
UniRef50_A0RV27 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 95 1e-18
UniRef50_UPI00015BB118 Cluster: tRNA (guanine-N(2)-)-methyltrans... 93 5e-18
UniRef50_A7AUB1 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 92 1e-17
UniRef50_Q8TYY7 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 92 2e-17
UniRef50_Q4N4W5 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 91 4e-17
UniRef50_A2SRK9 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;... 82 8e-17
UniRef50_Q4J947 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 83 3e-16
UniRef50_Q64CS6 Cluster: N(2)N(2)-dimethylguanosine tRNA methylt... 87 3e-16
UniRef50_A2BK15 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 87 3e-16
UniRef50_Q8SR99 Cluster: N2,N2-DIMETHYLGUANOSINE tRNA METHYLTRAN... 87 6e-16
UniRef50_A0B927 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;... 86 1e-15
UniRef50_Q5CQU6 Cluster: Trm1p. N2,N2-dimethylguanosine tRNA met... 83 6e-15
UniRef50_A7D179 Cluster: tRNA (Guanine-N(2)-)-methyltransferase;... 83 1e-14
UniRef50_P57706 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 82 1e-14
UniRef50_Q2FN43 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;... 77 2e-13
UniRef50_Q6L2J7 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 72 6e-13
UniRef50_Q4BYN1 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;... 74 3e-12
UniRef50_Q9YDY7 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 74 4e-12
UniRef50_P57705 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 72 1e-11
UniRef50_Q0IBQ7 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 72 2e-11
UniRef50_Q4YVY3 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 67 4e-10
UniRef50_A3DNY4 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 67 4e-10
UniRef50_Q8NC68 Cluster: Uncharacterized protein C1orf25; n=46; ... 66 1e-09
UniRef50_A5JZI1 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 64 3e-09
UniRef50_Q24FJ5 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 62 1e-08
UniRef50_Q74ML2 Cluster: NEQ108; n=1; Nanoarchaeum equitans|Rep:... 61 3e-08
UniRef50_Q31KF9 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;... 61 3e-08
UniRef50_Q8IEC3 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 60 4e-08
UniRef50_Q9LYL0 Cluster: N2, N2-dimethylguanosine tRNA methyltra... 60 8e-08
UniRef50_UPI0000E49722 Cluster: PREDICTED: hypothetical protein,... 59 1e-07
UniRef50_Q00YF6 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 54 5e-06
UniRef50_UPI000155D1C2 Cluster: PREDICTED: similar to TRM1 tRNA ... 49 1e-04
UniRef50_Q8CX51 Cluster: tRNA (uracil-5-)-methyltransferase (EC ... 46 8e-04
UniRef50_Q6LLU2 Cluster: tRNA (uracil-5-)-methyltransferase (EC ... 42 0.013
UniRef50_Q31JA4 Cluster: tRNA (uracil-5-)-methyltransferase (EC ... 40 0.051
UniRef50_A5URP6 Cluster: PUA domain containing protein; n=5; Chl... 40 0.068
UniRef50_Q8R9R9 Cluster: Predicted SAM-dependent methyltransfera... 40 0.089
UniRef50_Q197B1 Cluster: Putative uncharacterized protein; n=1; ... 39 0.16
UniRef50_Q8GDQ7 Cluster: Methyltransferase; n=1; Heliobacillus m... 38 0.21
UniRef50_Q011B2 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 38 0.21
UniRef50_P39541 Cluster: Uncharacterized protein YJL195C; n=1; S... 37 0.48
UniRef50_UPI00006CA4AE Cluster: hypothetical protein TTHERM_0049... 37 0.63
UniRef50_Q756L4 Cluster: AER240Wp; n=1; Eremothecium gossypii|Re... 36 0.83
UniRef50_UPI0001509CE7 Cluster: cyclic nucleotide-binding domain... 36 1.1
UniRef50_Q1VMM5 Cluster: Ribosomal protein L11 methyltransferase... 36 1.1
UniRef50_A4ST99 Cluster: Putative uncharacterized protein; n=2; ... 36 1.1
UniRef50_Q7QPA8 Cluster: GLP_122_5076_6419; n=1; Giardia lamblia... 36 1.1
UniRef50_A1SHQ9 Cluster: Regulatory protein, TetR; n=1; Nocardio... 36 1.5
UniRef50_Q5VNJ0 Cluster: Putative tRNA-(N1G37) methyltransferase... 35 2.5
UniRef50_Q5PK68 Cluster: tRNA (uracil-5-)-methyltransferase (EC ... 35 2.5
UniRef50_Q749W6 Cluster: Putative uncharacterized protein; n=4; ... 34 3.4
UniRef50_A6QBC0 Cluster: tRNA (Uracil-5-)-methyltransferase; n=1... 34 3.4
UniRef50_A6EDS8 Cluster: PUA domain containing protein; n=1; Ped... 34 3.4
UniRef50_Q0W088 Cluster: Putative SAM-dependent methyltransferas... 34 3.4
UniRef50_Q7ULT2 Cluster: HemK protein; n=1; Pirellula sp.|Rep: H... 34 4.4
UniRef50_Q4E860 Cluster: Uncharacterized ACR, YhhQ family COG173... 34 4.4
UniRef50_A6EWY0 Cluster: Putative uncharacterized protein; n=1; ... 34 4.4
UniRef50_A4J7F1 Cluster: Ribosomal protein L11 methyltransferase... 34 4.4
UniRef50_A0C933 Cluster: Chromosome undetermined scaffold_16, wh... 34 4.4
UniRef50_UPI00006CAA99 Cluster: ATPase, histidine kinase-, DNA g... 33 5.9
UniRef50_A6LYS3 Cluster: Sensor protein; n=1; Clostridium beijer... 33 5.9
UniRef50_A6C404 Cluster: Putative uncharacterized protein; n=1; ... 33 5.9
UniRef50_Q01C22 Cluster: TRNA methyltransferase; n=1; Ostreococc... 33 5.9
UniRef50_Q61PE0 Cluster: Putative uncharacterized protein CBG075... 33 5.9
UniRef50_A7RS85 Cluster: Predicted protein; n=1; Nematostella ve... 33 5.9
UniRef50_Q8TYV2 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 33 5.9
UniRef50_A5IYW7 Cluster: Putative uncharacterized protein; n=1; ... 33 7.8
UniRef50_Q9LVG3 Cluster: Similarity to unknown protein; n=6; Ara... 33 7.8
UniRef50_A0B5V5 Cluster: Putative RNA methylase, NOL1/NOP2/sun f... 33 7.8
>UniRef50_UPI0000D56C3B Cluster: PREDICTED: similar to Probable
N(2),N(2)-dimethylguanosine tRNA methyltransferase
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=2;
Endopterygota|Rep: PREDICTED: similar to Probable
N(2),N(2)-dimethylguanosine tRNA methyltransferase
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Tribolium
castaneum
Length = 501
Score = 279 bits (685), Expect = 4e-74
Identities = 148/229 (64%), Positives = 171/229 (74%)
Frame = +1
Query: 79 IKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDE 258
I EG A+I T VFYNPVQEFNRDLSI VL F +DYK + +TV
Sbjct: 14 ITEGLAKI-KTLGSVFYNPVQEFNRDLSIVVLNTFAKDYKGDS-----------QTVW-- 59
Query: 259 ESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSR 438
E+G + I+ILEALSATGLRSIRYAKE+ IIAND+S +AVE I+ NI N V
Sbjct: 60 EAGHKYDDGISILEALSATGLRSIRYAKEVKGVKEIIANDISIKAVEDIERNICDNGVED 119
Query: 439 IIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAV 618
++ SHDDA MLMYK++ +RF AIDLDPYGCPSIFLDSAVQ+I++GGLLLVTATDMAV
Sbjct: 120 LVVASHDDATMLMYKNRK-ERRFDAIDLDPYGCPSIFLDSAVQTIKEGGLLLVTATDMAV 178
Query: 619 LAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
LAGNSPETCY KYGA+SL+ K CHEMALRI+LQCIE HANRY RYI P+
Sbjct: 179 LAGNSPETCYSKYGAISLRMKACHEMALRILLQCIEAHANRYGRYIAPL 227
>UniRef50_Q9VK89 Cluster: Probable N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=4; Diptera|Rep:
Probable N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Drosophila
melanogaster (Fruit fly)
Length = 578
Score = 273 bits (670), Expect = 3e-72
Identities = 142/246 (57%), Positives = 171/246 (69%), Gaps = 13/246 (5%)
Frame = +1
Query: 67 NLKTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQ-KKLE 243
N I+E AEI ++ VFYNPVQEFNRDLSIA L ++ + E+ + KKQ KK++
Sbjct: 16 NENVIRERNAEI-VSGGNVFYNPVQEFNRDLSIAALNVYRQRLTKERSEKALKKQRKKVK 74
Query: 244 TVQDE------------ESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSE 387
+DE E+G E + ILEAL+ATGLRSIRYA+EI I+ANDLS
Sbjct: 75 EQEDEKTTPVPEDPPVYEAGTRYEDGLRILEALAATGLRSIRYAQEIAGVRQIVANDLSR 134
Query: 388 QAVETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQ 567
QAV +I NI HN+V +IE SH DA LMY P KRF A+DLDPYGCP+ FLD A+Q
Sbjct: 135 QAVASINTNIRHNKVEELIEPSHSDAMTLMYLSTQPEKRFDAVDLDPYGCPNRFLDGAMQ 194
Query: 568 SIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYS 747
+ DGGLLLVTATDMAVLAGN+PE CY KYG+V L+ KCCHEMALRI+L CIE HANRY
Sbjct: 195 CLVDGGLLLVTATDMAVLAGNAPEACYVKYGSVPLRMKCCHEMALRILLHCIESHANRYG 254
Query: 748 RYIVPI 765
+YI P+
Sbjct: 255 KYIEPL 260
>UniRef50_UPI00015B5CEF Cluster: PREDICTED: similar to
ENSANGP00000010185; n=1; Nasonia vitripennis|Rep:
PREDICTED: similar to ENSANGP00000010185 - Nasonia
vitripennis
Length = 529
Score = 268 bits (656), Expect = 1e-70
Identities = 133/229 (58%), Positives = 169/229 (73%)
Frame = +1
Query: 79 IKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDE 258
++EGQA+I L + VFYNPVQEFNRDLSIAVL+ Y E+ Q +++ +++
Sbjct: 13 LEEGQAKILLEKKNVFYNPVQEFNRDLSIAVLS----QYSKER-----SSQSSIDSAKEK 63
Query: 259 ESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSR 438
+ + + + +LEALSATGLRSIRYAKE+P I+AND+S +AVE IK N+ HN V
Sbjct: 64 QKDDSQKTGLRVLEALSATGLRSIRYAKEVPGMAEIVANDISIKAVEAIKRNVIHNGVEN 123
Query: 439 IIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAV 618
I+ +DDA M+MY+++ +F A+DLDPYGCPSIFLD AVQ I+D GLLLVTATDMAV
Sbjct: 124 IVRPHNDDATMVMYQNRRT--KFDAVDLDPYGCPSIFLDGAVQCIKDNGLLLVTATDMAV 181
Query: 619 LAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
LAGNSPETCY KYGAVSLK+K CHEMALRI+LQ I HA RY + + P+
Sbjct: 182 LAGNSPETCYVKYGAVSLKSKSCHEMALRILLQHIASHAGRYGKAMTPL 230
>UniRef50_A7RIG7 Cluster: Predicted protein; n=2; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 607
Score = 249 bits (609), Expect = 7e-65
Identities = 121/231 (52%), Positives = 164/231 (70%), Gaps = 2/231 (0%)
Frame = +1
Query: 79 IKEGQAEICLTTEK-VFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQD 255
I+EG+AEI EK VFYNPVQEFNRD+S AV+ L ED R ++K + +
Sbjct: 24 IREGKAEIIFPAEKAVFYNPVQEFNRDISSAVIRLVCEDIDKWTKRRNDEKGDADKLTRA 83
Query: 256 EESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVS 435
E+ + ++TILE L+A+GLRS+RYA E+P ++IAND+S A I++N++HN+V+
Sbjct: 84 EKRAKDNLAQVTILEGLAASGLRSVRYALEVPGIHHVIANDISADAFSLIENNVKHNKVN 143
Query: 436 RIIETSHDDACMLMYKHKHP-SKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDM 612
I+ S +DA +LMY++++P S+RF IDLDPYG + FLD AVQ++ DGG+LLVT TDM
Sbjct: 144 HIVSASKNDASLLMYQYRYPASRRFTFIDLDPYGTAAPFLDGAVQAVADGGVLLVTCTDM 203
Query: 613 AVLAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
VL GN + CY KYG +SLK K CHEMALR++L IE HANRY RYI+P+
Sbjct: 204 GVLCGNHNDACYGKYGGMSLKAKFCHEMALRLLLSSIESHANRYKRYILPL 254
>UniRef50_UPI0000E48B85 Cluster: PREDICTED: hypothetical protein;
n=2; Strongylocentrotus purpuratus|Rep: PREDICTED:
hypothetical protein - Strongylocentrotus purpuratus
Length = 556
Score = 235 bits (575), Expect = 9e-61
Identities = 125/242 (51%), Positives = 162/242 (66%), Gaps = 13/242 (5%)
Frame = +1
Query: 79 IKEGQAEICLTT-EKVFYNPVQEFNRDLSIAVLTLFIEDY---KAEKLA---RFEKKQK- 234
++EG+A I +VFYNP Q NRDLS+AV LF+ED+ K K++ R E K
Sbjct: 13 VEEGRARIVFPNPNEVFYNPAQVINRDLSLAVTGLFVEDFLQGKEIKVSYEYRDETKDTD 72
Query: 235 --KLETVQDEE---SGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVE 399
+ ETV + G E IT LEALSA+GLRS+R AKE+P ++AND SE+AV
Sbjct: 73 DTQQETVSENNVCYPGQKCEEGITFLEALSASGLRSVRIAKEVPGIKRVVANDFSEEAVR 132
Query: 400 TIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQD 579
I+ NI+ N V ++E SH DA +LMYKH+ F +DLDPYG + FLDS VQ++++
Sbjct: 133 DIERNIQFNNVEDLVEASHSDASLLMYKHRFKDS-FNVVDLDPYGSAAQFLDSGVQAVRN 191
Query: 580 GGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIV 759
GGLL VT TDMAVL GN C+ KYGA++L+TK C+EMALRI+LQCIE A RY+RYIV
Sbjct: 192 GGLLCVTCTDMAVLCGNHGHACFAKYGAMALRTKFCNEMALRILLQCIETQATRYNRYIV 251
Query: 760 PI 765
P+
Sbjct: 252 PV 253
>UniRef50_Q23270 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=2;
Caenorhabditis|Rep: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Caenorhabditis
elegans
Length = 526
Score = 233 bits (569), Expect = 5e-60
Identities = 121/243 (49%), Positives = 164/243 (67%), Gaps = 3/243 (1%)
Frame = +1
Query: 46 MRMETISNLKTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEK 225
++ E + I+EGQA++ VFYNPVQEFNRDL++ VL F D+ +K A E+
Sbjct: 13 IKSEDKEEVTVIQEGQAKVGFHGP-VFYNPVQEFNRDLTVTVLRQFSADH--QKWAE-EQ 68
Query: 226 KQKKLETVQDEESGG---NPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAV 396
KQ K E ++ N + KI IL+ALSA+GLR++R++KE+P I+AND S+ AV
Sbjct: 69 KQLKTEEEPPKKKNKLAINEDGKIRILDALSASGLRALRFSKEVPNVGFIMANDFSDNAV 128
Query: 397 ETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQ 576
+I+ N++ N V I+E DA M M +H+ KRF A+DLDPYG S FLDSAVQ +
Sbjct: 129 ASIQENVKLNGVEDIVEAHFGDAVMTMMEHRGIDKRFHAVDLDPYGTASTFLDSAVQCVA 188
Query: 577 DGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYI 756
D G+L+VT TDMAVL GN+PE CY KY AV+ + KCCHE+ LRI+L+ I+ ANRY+RYI
Sbjct: 189 DRGILMVTCTDMAVLCGNTPEACYNKYDAVTTRMKCCHEVGLRILLRAIDSAANRYTRYI 248
Query: 757 VPI 765
P+
Sbjct: 249 EPL 251
>UniRef50_Q9NXH9-2 Cluster: Isoform 2 of Q9NXH9 ; n=2;
Homo/Pan/Gorilla group|Rep: Isoform 2 of Q9NXH9 - Homo
sapiens (Human)
Length = 630
Score = 225 bits (551), Expect = 7e-58
Identities = 120/258 (46%), Positives = 171/258 (66%), Gaps = 28/258 (10%)
Frame = +1
Query: 76 TIKEGQAEICL-TTEKVFYNPVQEFNRDLSIAVLTLFI----------------EDYKAE 204
T+ EG A+I + +VFYNPVQEFNRDL+ AV+T F +D +
Sbjct: 56 TVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKV 115
Query: 205 KLARFEKKQKKLETVQDEE--SGGNP---------EPKITILEALSATGLRSIRYAKEIP 351
+ E++++K+E + E SG P E + +LE L+A+GLRSIR+A E+P
Sbjct: 116 VVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVP 175
Query: 352 YATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPY 531
+++AND S +AV+ I+ N++ N V+ +++ S DA MLMY+H+ S+RF IDLDPY
Sbjct: 176 GLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPY 235
Query: 532 GCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIM 711
G P+ FLD+AVQ++ +GGLL VT TDMAVLAGNS ETCY KYGA++LK++ CHEMALRI+
Sbjct: 236 GSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIV 295
Query: 712 LQCIEQHANRYSRYIVPI 765
L ++ AN Y R++VP+
Sbjct: 296 LHSLDLRANCYQRFVVPL 313
>UniRef50_Q9NXH9 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=17;
Euteleostomi|Rep: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Homo sapiens
(Human)
Length = 659
Score = 225 bits (551), Expect = 7e-58
Identities = 120/258 (46%), Positives = 171/258 (66%), Gaps = 28/258 (10%)
Frame = +1
Query: 76 TIKEGQAEICL-TTEKVFYNPVQEFNRDLSIAVLTLFI----------------EDYKAE 204
T+ EG A+I + +VFYNPVQEFNRDL+ AV+T F +D +
Sbjct: 56 TVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKV 115
Query: 205 KLARFEKKQKKLETVQDEE--SGGNP---------EPKITILEALSATGLRSIRYAKEIP 351
+ E++++K+E + E SG P E + +LE L+A+GLRSIR+A E+P
Sbjct: 116 VVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVP 175
Query: 352 YATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPY 531
+++AND S +AV+ I+ N++ N V+ +++ S DA MLMY+H+ S+RF IDLDPY
Sbjct: 176 GLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPY 235
Query: 532 GCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIM 711
G P+ FLD+AVQ++ +GGLL VT TDMAVLAGNS ETCY KYGA++LK++ CHEMALRI+
Sbjct: 236 GSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIV 295
Query: 712 LQCIEQHANRYSRYIVPI 765
L ++ AN Y R++VP+
Sbjct: 296 LHSLDLRANCYQRFVVPL 313
>UniRef50_A6QP09 Cluster: LOC539613 protein; n=2; Bos taurus|Rep:
LOC539613 protein - Bos taurus (Bovine)
Length = 691
Score = 220 bits (538), Expect = 3e-56
Identities = 120/258 (46%), Positives = 170/258 (65%), Gaps = 28/258 (10%)
Frame = +1
Query: 76 TIKEGQAEICL-TTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARF----EKKQKKL 240
TI EG A I +VFYNPVQEFNRDL+ AV+T F A K + EK +K+
Sbjct: 87 TITEGAARIVFPNANEVFYNPVQEFNRDLTCAVITEFARIQLAAKGIQIKVPGEKDVQKV 146
Query: 241 -----ETVQDE---ESGGN----PEPK-----------ITILEALSATGLRSIRYAKEIP 351
E +D+ + G N +P+ + +LE L+A+GLRSIR+A+E+P
Sbjct: 147 VVDLSEPTEDKAELKEGANLALEDQPRTAAVGEICEEGLRVLEGLAASGLRSIRFAREVP 206
Query: 352 YATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPY 531
+++AND S +AV+ ++ N++ N+V+ +++ S DA MLMY+H+ S+RF IDLDPY
Sbjct: 207 GLRSVVANDASARAVDLMRRNVQLNEVAHLVQPSQADARMLMYQHQKASERFDVIDLDPY 266
Query: 532 GCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIM 711
G P+ FLD+AVQ++ +GGLL VT TDMAVLAGNS ETCY KYGA++LK++ CHEMALR +
Sbjct: 267 GSPASFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRTV 326
Query: 712 LQCIEQHANRYSRYIVPI 765
L ++ AN Y R++VP+
Sbjct: 327 LHSLDLRANCYQRFVVPL 344
>UniRef50_Q54PG3 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 604
Score = 212 bits (517), Expect = 9e-54
Identities = 121/238 (50%), Positives = 158/238 (66%), Gaps = 4/238 (1%)
Frame = +1
Query: 64 SNLKTIKEGQAEICLTTE-KVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKL 240
SN+ T+ E A I + +VFYNPVQEFNRD+SI ++ LFIE+ K E L R
Sbjct: 49 SNIVTVIENSATILYNNQNEVFYNPVQEFNRDMSILMIKLFIEERKKECLER-------- 100
Query: 241 ETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATN-IIANDLSEQAVETIKHNI 417
G P KI +LEAL+ATGLRSIRYAKEI + I+AND+ + AV++I N
Sbjct: 101 ---------GKPFKKIRVLEALAATGLRSIRYAKEIGDDLDYILANDILQTAVDSIVKNR 151
Query: 418 EHNQV-SRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLL 594
E+N V I+ + DA M+M +++ SK++ +D+DPYG P+ FLDSAVQ++ DGGLL
Sbjct: 152 EYNGVPEERIKPNLGDATMVMMENRTHSKQYDVVDVDPYGAPTQFLDSAVQAVADGGLLC 211
Query: 595 VTATDMAVLAGNSPETCYCKYGAVSL-KTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
VTATD AVL G+ PE C+ KY +V + + CHEM LRI+L IEQHANRY R+IVPI
Sbjct: 212 VTATDTAVLCGSYPEACFHKYQSVPVHRAGFCHEMGLRILLHSIEQHANRYKRHIVPI 269
>UniRef50_P15565 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase, mitochondrial precursor (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=9;
Saccharomycetales|Rep: N(2),N(2)-dimethylguanosine tRNA
methyltransferase, mitochondrial precursor (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Saccharomyces
cerevisiae (Baker's yeast)
Length = 570
Score = 209 bits (511), Expect = 5e-53
Identities = 115/257 (44%), Positives = 168/257 (65%), Gaps = 22/257 (8%)
Frame = +1
Query: 61 ISNLKTIKEGQAEICLTT-EKVFYNPVQEFNRDLSIAVL----TLFIEDYKAEKLARFEK 225
I + +KEG+AEI E VFYNP+Q+FNRDLS+ + L+ E+ ++ + K
Sbjct: 39 IEDFNIVKEGKAEILFPKKETVFYNPIQQFNRDLSVTCIKAWDNLYGEECGQKRNNKKSK 98
Query: 226 KQKKLETVQD------------EESGGNP---EPKITILEALSATGLRSIRYAKEIPYAT 360
K++ ET D +E+ GN EP I ILEALSATGLR+IRYA EIP+
Sbjct: 99 KKRCAETNDDSSKRQKMGNGSPKEAVGNSNRNEPYINILEALSATGLRAIRYAHEIPHVR 158
Query: 361 NIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCP 540
+IANDL +AVE+IK N+E+N V I++ + DDA +LMY++K + +F IDLDPYG
Sbjct: 159 EVIANDLLPEAVESIKRNVEYNSVENIVKPNLDDANVLMYRNKATNNKFHVIDLDPYGTV 218
Query: 541 SIFLDSAVQSIQDGGLLLVTATDMAVLAGNS-PETCYCKYGAVSLKT-KCCHEMALRIML 714
+ F+D+A+QSI++GGL+LVT TD++VLAGN PE C+ YG ++ + + HE ALR++L
Sbjct: 219 TPFVDAAIQSIEEGGLMLVTCTDLSVLAGNGYPEKCFALYGGANMVSHESTHESALRLVL 278
Query: 715 QCIEQHANRYSRYIVPI 765
++Q A +Y + + P+
Sbjct: 279 NLLKQTAAKYKKTVEPL 295
>UniRef50_Q6C410 Cluster: Similar to sp|P15565 Saccharomyces
cerevisiae TRM1 N; n=1; Yarrowia lipolytica|Rep: Similar
to sp|P15565 Saccharomyces cerevisiae TRM1 N - Yarrowia
lipolytica (Candida lipolytica)
Length = 492
Score = 202 bits (494), Expect = 6e-51
Identities = 113/231 (48%), Positives = 152/231 (65%), Gaps = 3/231 (1%)
Frame = +1
Query: 79 IKEGQAEICLTTE-KVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQD 255
I EG+A I E +VFYNPVQ+FNRDLS+ + + E + EK A+ KK+K+ E ++
Sbjct: 10 ITEGKANILFPKENQVFYNPVQQFNRDLSVTCIRAWSEIWAEEK-AKKNKKRKRDEK-EN 67
Query: 256 EESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVS 435
EESG P+ ILEALSA+GLR+IRYAKEIP NIIAND S+ AV +I+ N+ HN+V
Sbjct: 68 EESGKPHIPEFNILEALSASGLRAIRYAKEIPGVKNIIANDFSKDAVASIERNVAHNKVE 127
Query: 436 RIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMA 615
++ + DA ++MY K+F IDLDPYG + F+D+AV+S+ + GLLLVT TD+A
Sbjct: 128 ELVTPNESDANLVMY-----GKQFQVIDLDPYGSATPFIDAAVRSVDEDGLLLVTCTDLA 182
Query: 616 VLAGNS-PETCYCKYGAVSLK-TKCCHEMALRIMLQCIEQHANRYSRYIVP 762
VLAGNS PE C+ YG + HE ALR++L I A +Y + I P
Sbjct: 183 VLAGNSHPEKCFSSYGGQTFHGGDATHESALRLVLHMIAGTAAKYGKCIEP 233
>UniRef50_Q339G6 Cluster: N-dimethylguanosine tRNA
methyltransferase, putative, expressed; n=2; Oryza
sativa (japonica cultivar-group)|Rep:
N-dimethylguanosine tRNA methyltransferase, putative,
expressed - Oryza sativa subsp. japonica (Rice)
Length = 555
Score = 192 bits (468), Expect = 8e-48
Identities = 117/246 (47%), Positives = 147/246 (59%), Gaps = 16/246 (6%)
Frame = +1
Query: 76 TIKEGQAEICLT-TEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEK-KQKKLETV 249
TI+EG+AEI + VFYN Q NRDLSIAVL FI + E + + +L
Sbjct: 53 TIREGRAEIFADDSNSVFYNKAQVNNRDLSIAVLRSFISRRREEHDIQLRRGSHAELPPK 112
Query: 250 QD-EESG---GNPEPKIT----------ILEALSATGLRSIRYAKEIPYATNIIANDLSE 387
EE G G+ E K +LEAL+A+GLR+IRYA E+ +IA D +E
Sbjct: 113 HHAEELGHIRGSSEDKALSEEISYRAPKVLEALAASGLRAIRYALEVDGIGEVIAVDNNE 172
Query: 388 QAVETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQ 567
A+E +K NI HN + A +Y HP K F +DLDPYG P+ FLDSAVQ
Sbjct: 173 VAIEAVKKNIHHNGSVASSKVVPHLADARVYMLTHP-KEFDVVDLDPYGSPAAFLDSAVQ 231
Query: 568 SIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYS 747
+ DGG+L+ +ATDMAVLAG + E C+ KYG+ LK K CHEMALRI+L CIE HA R+
Sbjct: 232 CVADGGILMCSATDMAVLAGGNAEVCFSKYGSYPLKGKHCHEMALRILLACIESHAIRHK 291
Query: 748 RYIVPI 765
RYIVPI
Sbjct: 292 RYIVPI 297
>UniRef50_Q5C276 Cluster: SJCHGC07232 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC07232 protein - Schistosoma
japonicum (Blood fluke)
Length = 222
Score = 183 bits (446), Expect = 4e-45
Identities = 104/218 (47%), Positives = 141/218 (64%)
Frame = +1
Query: 61 ISNLKTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKL 240
++N +KEG AE+ L + VFYNPVQEFNRDL+IA++ F + ++ E KL
Sbjct: 15 MTNPSFVKEGSAEVMLP-DAVFYNPVQEFNRDLTIAMVNQFRKLHEREAAV-------KL 66
Query: 241 ETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIE 420
+ + SG KI LEALSA+G+RSIR A E+P ++IIAND+S +AV I N
Sbjct: 67 KNNLNGTSGLIQGFKI--LEALSASGIRSIRMALEVPNVSSIIANDISPEAVSLIAKNAA 124
Query: 421 HNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVT 600
HN V+ I+ DA LM +H+ S+RF +D+DP+G S FLDSAVQ + GGLL VT
Sbjct: 125 HNNVASIVNPVCYDAIELMLQHRSHSERFNVVDIDPFGTASPFLDSAVQCLHGGGLLCVT 184
Query: 601 ATDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIML 714
+TDMAVL G++P T KYG +++KT HE+ LRI+L
Sbjct: 185 STDMAVLCGSTPGTSMGKYGGIAIKTGSTHEVGLRILL 222
>UniRef50_UPI00004990C0 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase; n=1; Entamoeba histolytica
HM-1:IMSS|Rep: N2,N2-dimethylguanosine tRNA
methyltransferase - Entamoeba histolytica HM-1:IMSS
Length = 489
Score = 179 bits (435), Expect = 8e-44
Identities = 99/238 (41%), Positives = 145/238 (60%), Gaps = 4/238 (1%)
Frame = +1
Query: 64 SNLKTIKEGQAEICLTTEKV--FYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKK 237
S IKEGQA + E+ FYNPVQE NRDLSI++++ ++ + + E KQK
Sbjct: 7 SKYTPIKEGQATVLFPKEEAGAFYNPVQELNRDLSISIISKYLRNKENEL------KQK- 59
Query: 238 LETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATN--IIANDLSEQAVETIKH 411
N + I+EAL+A+GLRS+RYAKE+P + +IAND+S+ AVE+IK
Sbjct: 60 -----------NKQYHPLIVEALAASGLRSVRYAKELPQDIDFKVIANDISQSAVESIKR 108
Query: 412 NIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLL 591
N +N ++I + S DA +L+Y+ ++ IDLDPYG P++FLD+A +I+D GL+
Sbjct: 109 NATYNNTTKI-QPSESDAVLLLYQLSQQKEKPDVIDLDPYGAPTVFLDAAFAAIKDDGLM 167
Query: 592 LVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
VT TDMA LAG C+ KYG + + HE LR+ L + ++A R+ +YIVP+
Sbjct: 168 AVTCTDMACLAGTHSPACFAKYGGMPWHSSSAHEFGLRLALHSLAENAARHKKYIVPL 225
>UniRef50_Q9P804 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=1;
Schizosaccharomyces pombe|Rep:
N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC
2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase)
(tRNA 2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) -
Schizosaccharomyces pombe (Fission yeast)
Length = 548
Score = 176 bits (429), Expect = 4e-43
Identities = 107/255 (41%), Positives = 147/255 (57%), Gaps = 25/255 (9%)
Frame = +1
Query: 76 TIKEGQAEICLTT-EKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQK------ 234
++ EG A I + +VFYNPVQ+FNRDLS+ + + E + +A+ + +
Sbjct: 31 SLTEGSAIIPFSNPNEVFYNPVQQFNRDLSVTAIRAWSETRSKKIVAKSHHRHQLLDQNS 90
Query: 235 --------KLETVQDEESGGNPEPK---------ITILEALSATGLRSIRYAKEIPYATN 363
K +T D E + + TILEALSATGLRSIRYAKE+P
Sbjct: 91 ELSQENLTKCDTTHDFEKNCSEKTDSTSADNIAGFTILEALSATGLRSIRYAKELPNVKR 150
Query: 364 IIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPS 543
I+ANDL E AV+TI+ N+ +N VS I+ + DA +M+ +K + IDLDPYG +
Sbjct: 151 ILANDLLENAVKTIEKNVNYNNVSDIVIPNKGDANAVMHMNKF---HYDVIDLDPYGSAA 207
Query: 544 IFLDSAVQSIQDGGLLLVTATDMAVLAGNS-PETCYCKYGAVSLKTKCCHEMALRIMLQC 720
FLD+AVQS+ GLL +T TD AVLAGN+ PE C+ YG SL++ CHE A+R +L
Sbjct: 208 PFLDAAVQSVSKDGLLCITCTDSAVLAGNAYPEKCFSNYGGSSLRSNFCHEQAVRHLLYA 267
Query: 721 IEQHANRYSRYIVPI 765
I A +Y R I P+
Sbjct: 268 IAASAAKYGRAIKPL 282
>UniRef50_Q9SRU7 Cluster: Probable N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=13;
Viridiplantae|Rep: Probable N(2),N(2)-dimethylguanosine
tRNA methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Arabidopsis
thaliana (Mouse-ear cress)
Length = 599
Score = 175 bits (427), Expect = 8e-43
Identities = 89/162 (54%), Positives = 111/162 (68%), Gaps = 2/162 (1%)
Frame = +1
Query: 286 ITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRI--IETSHD 459
I I +ALSA+GLR++RYA+EI ++A D +VE + NI+ N I +E+ H
Sbjct: 132 ILIFQALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVAISKVESHHT 191
Query: 460 DACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPE 639
DA + M H K F +DLDPYG PSIFLDSA+QS+ DGGLL+ TATDMAVL G + E
Sbjct: 192 DARVHMLTHP---KEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVLCGGNGE 248
Query: 640 TCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
CY KYG+ L+ K CHEMALRI+L IE HANRY RYIVP+
Sbjct: 249 VCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPV 290
Score = 48.0 bits (109), Expect = 3e-04
Identities = 28/73 (38%), Positives = 45/73 (61%), Gaps = 2/73 (2%)
Frame = +1
Query: 52 MET-ISNLKTIKEGQAEICLTTE-KVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEK 225
MET +++ IKEG+AEI + + +VF+N Q NRD+SIAVL F+ K E A+ K
Sbjct: 1 METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60
Query: 226 KQKKLETVQDEES 264
+ + V ++++
Sbjct: 61 RTRPASKVIEKDA 73
>UniRef50_A1CC48 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase, putative; n=2; Aspergillus|Rep:
N2,N2-dimethylguanosine tRNA methyltransferase, putative
- Aspergillus clavatus
Length = 1045
Score = 163 bits (396), Expect = 4e-39
Identities = 103/257 (40%), Positives = 145/257 (56%), Gaps = 39/257 (15%)
Frame = +1
Query: 112 TEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKL----ETVQDEESG---- 267
++ VFYNP+Q+FNRDLS+ + + E A K + EKK+ + + +D+E+G
Sbjct: 391 SQSVFYNPIQQFNRDLSVLAIKAYGEHLLALKKLKAEKKRSGVGRGKKRKRDDEAGTEQP 450
Query: 268 ----------GN-----PEPK-----ITILEALSATGLRSIRYAKEIPYATNIIANDLSE 387
GN P P+ TIL+ALSATGLR++RYA EIP+ T ++ANDLS
Sbjct: 451 ATSEELPGANGNSTASAPHPEQSVIPFTILDALSATGLRALRYASEIPFVTCVVANDLSS 510
Query: 388 QAVETIKHNIEHNQVSRIIETSHDDACMLMY----------KHKHPSKRFAAIDLDPYGC 537
A++++K NIE+N + ++I + DA MY H SK F IDLDPYG
Sbjct: 511 SAIQSMKTNIEYNDLGKLIRPNLGDARAYMYSILNQTSTPGSGTHTSK-FDVIDLDPYGT 569
Query: 538 PSIFLDSAVQSIQDGGLLLVTATDMAVLAGNS-PETCYCKYGAVSLKTKCCHEMALRIML 714
+ F+D+AVQ+++DGGLL VT TD V A N PE Y YG V +K HE LR++L
Sbjct: 570 AASFMDAAVQAVKDGGLLCVTCTDAGVWASNGYPEKAYALYGGVPIKGSHSHEGGLRLIL 629
Query: 715 QCIEQHANRYSRYIVPI 765
+ A +Y I P+
Sbjct: 630 HALATSAAKYGLAIEPL 646
>UniRef50_Q5A863 Cluster: Likely N2,N2-dimethylguanosine-specific
tRNA methyltransferase; n=5; Saccharomycetales|Rep:
Likely N2,N2-dimethylguanosine-specific tRNA
methyltransferase - Candida albicans (Yeast)
Length = 556
Score = 161 bits (391), Expect = 2e-38
Identities = 96/251 (38%), Positives = 147/251 (58%), Gaps = 8/251 (3%)
Frame = +1
Query: 37 NIFMRMETISN-LKTIKEGQAEICLTTE-KVFYNPVQEFNRDLSIAVLTLFIEDYKAEKL 210
NI TIS T++EG+A I + +VFYNP+Q+FNRDLSI + + ++ + EK+
Sbjct: 28 NIVQPNSTISQEFNTVQEGKATILTPKQDEVFYNPIQQFNRDLSIMAIKAY-DEIRHEKI 86
Query: 211 ARFEKKQKKLETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQ 390
+KK K T + G + ILE+L+A+GLRS RY EIP A I+AND+ +
Sbjct: 87 QAIKKKSKNKRT----KLNG-----LKILESLAASGLRSCRYGLEIPEAGKIVANDMLAE 137
Query: 391 AVETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQS 570
AV++I N+E+N+++ + + DA M ++F +DLDPYG + F+DSA+Q
Sbjct: 138 AVKSINKNVEYNKLTDKVVANQGDAIKFM---GSTDEKFHIVDLDPYGTAAPFIDSAIQC 194
Query: 571 IQDGGLLLVTATDMAVLAGNS-PETCYCKYGA-----VSLKTKCCHEMALRIMLQCIEQH 732
++D G+LLVT TD VLAG+ PE C+ YG + + HE+ +R++L I
Sbjct: 195 LEDDGMLLVTCTDAGVLAGSGYPEKCFALYGGNNFGNAYVNGESNHEVGIRLILNLIAST 254
Query: 733 ANRYSRYIVPI 765
A +Y + I P+
Sbjct: 255 AAKYKKTIEPM 265
>UniRef50_Q4WEX8 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase; n=10; Trichocomaceae|Rep:
N2,N2-dimethylguanosine tRNA methyltransferase -
Aspergillus fumigatus (Sartorya fumigata)
Length = 747
Score = 159 bits (385), Expect = 9e-38
Identities = 101/256 (39%), Positives = 140/256 (54%), Gaps = 39/256 (15%)
Frame = +1
Query: 115 EKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQ--------KKLETVQDEE--- 261
+ VFYNP+Q+FNRDLS+ + + E A K + E+K+ +K E +D+E
Sbjct: 112 QSVFYNPIQQFNRDLSVLAIKAYGEHVLALKKLKAERKRNGSSRGKKRKREDEEDKEQDE 171
Query: 262 --------SGGN----------PEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSE 387
+ GN P TIL+ALSATGLR++RYA EIP+ T ++ANDLS
Sbjct: 172 VDVEQPSAANGNIGASVSTSDQQTPSFTILDALSATGLRALRYASEIPFTTCVVANDLSN 231
Query: 388 QAVETIKHNIEHNQVSRIIETSHDDACMLMYK-HKHPSK--------RFAAIDLDPYGCP 540
A++ +K NIE+N + ++I + DA MY PS +F IDLDPYG
Sbjct: 232 SAIQRMKTNIEYNGLGKLIRPNLGDARAYMYSLLNQPSSQNSGTHAGKFDVIDLDPYGTA 291
Query: 541 SIFLDSAVQSIQDGGLLLVTATDMAVLAGNS-PETCYCKYGAVSLKTKCCHEMALRIMLQ 717
+ F+D+AVQ ++DGGLL VT TD V A N PE Y YG V +K HE LR++L
Sbjct: 292 APFMDAAVQGVKDGGLLCVTCTDAGVWASNGYPEKAYALYGGVPIKGSHSHEGGLRLILH 351
Query: 718 CIEQHANRYSRYIVPI 765
+ A +Y I P+
Sbjct: 352 GLATSAAKYGLAIEPL 367
>UniRef50_A6RDC7 Cluster: Putative uncharacterized protein; n=1;
Ajellomyces capsulatus NAm1|Rep: Putative
uncharacterized protein - Ajellomyces capsulatus NAm1
Length = 797
Score = 159 bits (385), Expect = 9e-38
Identities = 88/196 (44%), Positives = 115/196 (58%), Gaps = 9/196 (4%)
Frame = +1
Query: 205 KLARFEKKQKKLETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLS 384
K R E++ T + N P +IL+ALSATGLR++RYAKEIP+AT I+ANDLS
Sbjct: 184 KCVRAEQQDTATGTTTTTVTEQNKIPPFSILDALSATGLRALRYAKEIPFATRIVANDLS 243
Query: 385 EQAVETIKHNIEHNQVSRIIETSHDDACMLMYK--HKHPSK------RFAAIDLDPYGCP 540
AV ++K NI++N V ++ + DDAC MY P K +F +DLDPYG
Sbjct: 244 PDAVRSMKLNIQYNGVGNVVMPNIDDACAYMYSMLRARPQKDGSHFGKFDVVDLDPYGTA 303
Query: 541 SIFLDSAVQSIQDGGLLLVTATDMAVLAGNS-PETCYCKYGAVSLKTKCCHEMALRIMLQ 717
+ FLD+AVQ+I DGGLL VT TD +V A N PE Y YG + K HE LR++L
Sbjct: 304 APFLDAAVQAICDGGLLCVTCTDASVFASNGYPEKTYALYGGLPFKGPQSHEAGLRLILH 363
Query: 718 CIEQHANRYSRYIVPI 765
I A +Y I P+
Sbjct: 364 AIASSAAKYGLSIEPL 379
Score = 40.7 bits (91), Expect = 0.039
Identities = 17/38 (44%), Positives = 24/38 (63%)
Frame = +1
Query: 112 TEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEK 225
T+ VFYNP+Q+FNRDLS+ + + E K + EK
Sbjct: 103 TQSVFYNPIQQFNRDLSVLAIRAYGEHALVVKREKMEK 140
>UniRef50_Q8RU67 Cluster: Putative tRNA
(Guanine-N2-)-Methyltransferase; n=3; Oryza sativa|Rep:
Putative tRNA (Guanine-N2-)-Methyltransferase - Oryza
sativa (Rice)
Length = 490
Score = 156 bits (379), Expect = 5e-37
Identities = 110/246 (44%), Positives = 137/246 (55%), Gaps = 16/246 (6%)
Frame = +1
Query: 76 TIKEGQAEICLT-TEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEK-KQKKLETV 249
TI+EG+AEI + VFYN Q NRDLSIAVL FI + E + + +L
Sbjct: 53 TIREGRAEIFADDSNSVFYNKAQVNNRDLSIAVLRSFISRRREEHDIQLRRGSHAELPPK 112
Query: 250 QD-EESG---GNPEPKIT----------ILEALSATGLRSIRYAKEIPYATNIIANDLSE 387
EE G G+ E K +LEAL+A+GLR+IRYA E+ +IA D
Sbjct: 113 HHAEELGHIRGSSEDKALSEEISYRAPKVLEALAASGLRAIRYALEVDGIGEVIAVD--- 169
Query: 388 QAVETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQ 567
N E N + T +YK K +DLDPYG P+ FLDSAVQ
Sbjct: 170 --------NNEGNNYNLATHT--------IYK-----KLVFKVDLDPYGSPAAFLDSAVQ 208
Query: 568 SIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYS 747
+ DGG+L+ +ATDMAVLAG + E C+ KYG+ LK K CHEMALRI+L CIE HA R+
Sbjct: 209 CVADGGILMCSATDMAVLAGGNAEVCFSKYGSYPLKGKHCHEMALRILLACIESHAIRHK 268
Query: 748 RYIVPI 765
RYIVPI
Sbjct: 269 RYIVPI 274
>UniRef50_Q1DMI8 Cluster: Putative uncharacterized protein; n=1;
Coccidioides immitis|Rep: Putative uncharacterized
protein - Coccidioides immitis
Length = 853
Score = 155 bits (376), Expect = 1e-36
Identities = 78/172 (45%), Positives = 108/172 (62%), Gaps = 10/172 (5%)
Frame = +1
Query: 280 PKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHD 459
P TIL+ALSATGLR++RYAKEIP+AT ++ANDLS A+ ++K+NI+HN+V I+ ++
Sbjct: 296 PSFTILDALSATGLRALRYAKEIPFATQVVANDLSALAIASMKNNIKHNRVDDIVRPNNG 355
Query: 460 DACMLMYKHKHPSK---------RFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDM 612
DAC MY P K +F +DLDPYG + F+D+AVQ++ DGG+L VT TD
Sbjct: 356 DACSYMYSILGPQKPHKDGSYFGKFDVVDLDPYGTAAPFMDAAVQAVTDGGMLCVTCTDA 415
Query: 613 AVLAGNS-PETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
V A PE Y YG + +K HE LR++L + A +Y I P+
Sbjct: 416 GVFAATGYPEKAYALYGGIPIKGVHSHEGGLRLILHALATSAAKYGIAIEPL 467
Score = 43.6 bits (98), Expect = 0.005
Identities = 19/42 (45%), Positives = 28/42 (66%)
Frame = +1
Query: 121 VFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLET 246
VFYNP+Q+FNRDLS+ + + E A K + EKK K+ ++
Sbjct: 194 VFYNPIQQFNRDLSVLAIRAYGEHAIAMKTEKHEKKLKRRDS 235
>UniRef50_Q0UJY7 Cluster: Putative uncharacterized protein; n=1;
Phaeosphaeria nodorum|Rep: Putative uncharacterized
protein - Phaeosphaeria nodorum (Septoria nodorum)
Length = 677
Score = 150 bits (363), Expect = 4e-35
Identities = 76/163 (46%), Positives = 105/163 (64%), Gaps = 1/163 (0%)
Frame = +1
Query: 280 PKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHD 459
P +IL+ALSATGLR++RYAKEIP+AT++ AND+S++AV +IK N++HN++ I
Sbjct: 204 PPFSILDALSATGLRALRYAKEIPFATSVTANDMSQKAVNSIKLNVKHNKLEDTITAKTG 263
Query: 460 DACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNS-P 636
+A MY + + IDLDPYG + FLDSA+Q+I GLL VT TD A+ A +
Sbjct: 264 NAIAYMYSY-CDKNGYDVIDLDPYGTAAPFLDSAIQAINSDGLLCVTCTDSAIFASHGYL 322
Query: 637 ETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
E Y +YG + LK + CHE LR++L I A RY I P+
Sbjct: 323 EKTYSQYGGLPLKGEPCHEGGLRLLLHAIASSAGRYGMAIEPL 365
Score = 46.0 bits (104), Expect = 0.001
Identities = 27/89 (30%), Positives = 47/89 (52%), Gaps = 1/89 (1%)
Frame = +1
Query: 115 EKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITI 294
++VFYNP+Q+FNRDLS+ + F +D K + E+ +KK E + + + K
Sbjct: 64 QQVFYNPIQQFNRDLSVLAIKTFGQDIIQRKTLKHEQLKKKGERKRQRK-----QEKAAA 118
Query: 295 LEALSATGLR-SIRYAKEIPYATNIIAND 378
EA ++T + + A ++P +A D
Sbjct: 119 EEAPNSTCVSDQVPVADKVPATKEAVATD 147
>UniRef50_Q7S9X0 Cluster: Putative uncharacterized protein
NCU06336.1; n=2; Sordariales|Rep: Putative
uncharacterized protein NCU06336.1 - Neurospora crassa
Length = 683
Score = 149 bits (360), Expect = 1e-34
Identities = 84/195 (43%), Positives = 117/195 (60%), Gaps = 15/195 (7%)
Frame = +1
Query: 226 KQKKLETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETI 405
+Q E E G +P T+L+ALSA+GLR++RYA EIP+ T++ ANDL AVE+I
Sbjct: 157 EQGDKEATNGEAKKGK-QPPFTVLDALSASGLRALRYAHEIPFLTSVTANDLLATAVESI 215
Query: 406 KHNIEHNQVSRIIETSHDDACMLMY-------KHKHP------SKRFAAIDLDPYGCPSI 546
K N++HN++ + HDDA MY + K P S+++ +DLDPYG +
Sbjct: 216 KLNVKHNRLEDKVNVCHDDALAHMYTLIAKELRSKDPKGKPTISEKYDVVDLDPYGTAAP 275
Query: 547 FLDSAVQSIQ-DGGLLLVTATDMAVLAGNS-PETCYCKYGAVSLKTKCCHEMALRIMLQC 720
FLD+AVQS++ DGGLL VT TD V A N PE CY YG V +K HE+ +R++L
Sbjct: 276 FLDAAVQSVRDDGGLLCVTCTDSGVWASNGYPEKCYSLYGGVPVKAWFSHEVGIRLILYS 335
Query: 721 IEQHANRYSRYIVPI 765
I+ A +Y I P+
Sbjct: 336 IQTAAAKYGLTIEPL 350
Score = 38.7 bits (86), Expect = 0.16
Identities = 15/42 (35%), Positives = 28/42 (66%)
Frame = +1
Query: 118 KVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLE 243
+VFYNP+Q++NRDL++ + + ++ +K A K +K +E
Sbjct: 56 QVFYNPIQQYNRDLTVLAIKAYGKEAVQQKQASHSKLEKIIE 97
>UniRef50_UPI000023D9FF Cluster: hypothetical protein FG09705.1;
n=1; Gibberella zeae PH-1|Rep: hypothetical protein
FG09705.1 - Gibberella zeae PH-1
Length = 1026
Score = 142 bits (345), Expect = 6e-33
Identities = 100/267 (37%), Positives = 145/267 (54%), Gaps = 50/267 (18%)
Frame = +1
Query: 115 EKVFYNPVQEFNRDLSIAVLTLF----IEDYKAEKLARFEK---KQKKLETVQ------- 252
++VFYNP+Q++NRDLS+ + + +E K + +R +K K+K+ + VQ
Sbjct: 44 QQVFYNPIQQYNRDLSVLAIKTYGEMSLEKRKEQYESRLKKQGKKRKRGDDVQKPNEDAA 103
Query: 253 -----DE----ESGGNPE------------PKITILEALSATGLRSIRYAKEIPYATNII 369
DE E NP+ P IL+ALSA+GLR++RYA E+P+ T++
Sbjct: 104 EPAPEDEPQVTEEAANPDVADPAQPKKEYKPAFRILDALSASGLRALRYAHELPFVTSVK 163
Query: 370 ANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMY-----------KHKHPSK--RFA 510
ANDLS+ A ++I+ N +HN + I + DA LMY K P K +F
Sbjct: 164 ANDLSDTAADSIRMNAKHNGLDDKINVTQGDALALMYRGIADDLSSRDKAGSPGKANKFD 223
Query: 511 AIDLDPYGCPSIFLDSAVQSIQ-DGGLLLVTATDMAVLAGNS-PETCYCKYGAVSLKTKC 684
IDLDPYG + F D+AVQS++ DGGLL +T TD AV AG+S E + YG + +K
Sbjct: 224 VIDLDPYGTAAPFFDAAVQSVKDDGGLLCITCTDSAVWAGHSYCEKTFALYGGIPIKGMH 283
Query: 685 CHEMALRIMLQCIEQHANRYSRYIVPI 765
HE LR++L + A RY I P+
Sbjct: 284 SHEAGLRLVLNAVATSAARYGLTIEPL 310
>UniRef50_A6RZH6 Cluster: Putative uncharacterized protein; n=2;
Sclerotiniaceae|Rep: Putative uncharacterized protein -
Botryotinia fuckeliana B05.10
Length = 688
Score = 138 bits (335), Expect = 1e-31
Identities = 79/175 (45%), Positives = 109/175 (62%), Gaps = 10/175 (5%)
Frame = +1
Query: 271 NP-EPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIE 447
NP P+ IL+ALSATGLR++RYA+EIP+AT+I ANDL A +TI+ N++HN++ I+
Sbjct: 197 NPINPRFQILDALSATGLRALRYAQEIPFATSITANDLLPAATKTIELNVKHNKLENKIK 256
Query: 448 TSHDDACMLMY-------KHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQ-DGGLLLVTA 603
+A MY K S+++ IDLDPYG + FLD+AVQ+++ DGGLL VT
Sbjct: 257 AVTGNALTHMYSLVGDDCKDGSGSRKYDVIDLDPYGTAAPFLDAAVQAVRDDGGLLCVTC 316
Query: 604 TDMAVLAGNS-PETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
TD V A N PE Y YG ++K + HE LR++L I A RY + P+
Sbjct: 317 TDAGVWASNGYPEKAYSLYGGTTVKGQHSHEGGLRLILHSIATSAARYGLAMEPL 371
Score = 40.3 bits (90), Expect = 0.051
Identities = 19/55 (34%), Positives = 33/55 (60%)
Frame = +1
Query: 73 KTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKK 237
+T +GQ ++ + VFYNP+Q+FNRDLS+ + + E +K E+ ++K
Sbjct: 46 QTTPKGQNQV----QSVFYNPIQQFNRDLSVLAIKAYGESILEKKKKIIERDRQK 96
>UniRef50_Q22HL7 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase family protein; n=1; Tetrahymena
thermophila SB210|Rep: N2,N2-dimethylguanosine tRNA
methyltransferase family protein - Tetrahymena
thermophila SB210
Length = 557
Score = 133 bits (322), Expect = 4e-30
Identities = 89/252 (35%), Positives = 129/252 (51%), Gaps = 7/252 (2%)
Frame = +1
Query: 31 INNIFMRMETISNLKTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKL 210
++N F++ K+ E + +VFYNPVQ FNRDL++ V+ +F E K +
Sbjct: 36 VDNTFIQEGYAKIQKSYIENSEDGKKLLSEVFYNPVQVFNRDLTVLVIHMFKEYLKETRK 95
Query: 211 ARFEKKQKKLETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQ 390
+F+ ITIL+ALSA+GLR+IR++KE+ + AND+SE
Sbjct: 96 EKFDG--------------------ITILDALSASGLRAIRFSKELKDIKKVYANDISEA 135
Query: 391 AVETIKHNIEHNQVSRI-IETSHDDACMLMYKHKHPSK------RFAAIDLDPYGCPSIF 549
+ + + N N + IE + DA LMY+ K S RF IDLDPYG + F
Sbjct: 136 SHKLMMDNFILNNLDLSKIEMTLKDAVQLMYEQKFRSSSGDRNARFDVIDLDPYGTCAPF 195
Query: 550 LDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQ 729
LD A+Q LL +T+TD +L G + CY YG+ K E A+RI+L
Sbjct: 196 LDCAIQGAVGETLLCITSTDSRILCGPDTQKCYYLYGSSRAKIAAYEENAVRIILYTANN 255
Query: 730 HANRYSRYIVPI 765
ANRY ++I P+
Sbjct: 256 IANRYGKFITPL 267
>UniRef50_A2FDH4 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase family protein; n=4; Trichomonas
vaginalis G3|Rep: N2,N2-dimethylguanosine tRNA
methyltransferase family protein - Trichomonas vaginalis
G3
Length = 476
Score = 132 bits (320), Expect = 7e-30
Identities = 86/215 (40%), Positives = 120/215 (55%)
Frame = +1
Query: 121 VFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITILE 300
VFYNPVQ+FNRDL+ AV I+ Y+ +V+D+ I+I E
Sbjct: 34 VFYNPVQQFNRDLTCAV----IQAYR--------------NSVRDD---------ISIFE 66
Query: 301 ALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMY 480
A +A+GLRSIRYAKEI II+NDL AVE IK NI N+VS I+ S DA M
Sbjct: 67 AFAASGLRSIRYAKEITGIKEIISNDLDPGAVEIIKRNIAINKVSNIVTASQGDARDKML 126
Query: 481 KHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYG 660
+ ++ ++ +DLDPY + FL++AV++ +G LL +T+TD L G P+ Y YG
Sbjct: 127 EEEN---KYQVLDLDPYSTAAPFLEAAVKAATNGALLCITSTDGRTLCGVQPDVAYSWYG 183
Query: 661 AVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
V L + HE +R +L + A+R R I P+
Sbjct: 184 CVPLNVEFEHEFGIRCLLTTLMNVASRLKRSIEPL 218
>UniRef50_Q4P6Q4 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 711
Score = 125 bits (302), Expect = 1e-27
Identities = 59/109 (54%), Positives = 75/109 (68%), Gaps = 1/109 (0%)
Frame = +1
Query: 442 IETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVL 621
++ + DA LMY H+ P KRF +DLDPYG + FLD VQS+ DGGLL VT TD+AVL
Sbjct: 315 VKLNAGDAITLMYTHREPHKRFDVVDLDPYGSAAPFLDGGVQSVADGGLLCVTCTDLAVL 374
Query: 622 AG-NSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
AG N PE C+ +YG VS+K + HE+ALR++L I A RY RYI P+
Sbjct: 375 AGHNYPEKCFSQYGGVSVKAEFSHEVALRLVLHTIATSAARYGRYIQPV 423
Score = 63.3 bits (147), Expect = 6e-09
Identities = 31/48 (64%), Positives = 37/48 (77%)
Frame = +1
Query: 283 KITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHN 426
+ T+LEALSATGLRSIRYAKEIP ++ANDLS AVE +K N+ N
Sbjct: 172 QFTLLEALSATGLRSIRYAKEIPLLRWVLANDLSPTAVEMMKRNVALN 219
Score = 45.6 bits (103), Expect = 0.001
Identities = 26/64 (40%), Positives = 36/64 (56%), Gaps = 2/64 (3%)
Frame = +1
Query: 82 KEGQAEICL-TTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARF-EKKQKKLETVQD 255
+E A I + + E F NPVQEFNRDLS + + E + AEK ARF Q+K + +
Sbjct: 28 RENSATIVMPSAEAAFLNPVQEFNRDLSTLAIRTWSERFDAEKKARFLRAAQRKAVSAAN 87
Query: 256 EESG 267
+ G
Sbjct: 88 KGKG 91
>UniRef50_A0CLP3 Cluster: Chromosome undetermined scaffold_20, whole
genome shotgun sequence; n=1; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_20,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 487
Score = 122 bits (293), Expect = 1e-26
Identities = 87/220 (39%), Positives = 115/220 (52%)
Frame = +1
Query: 106 LTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPK 285
L E+VFYNP Q NRDL++ V+ +T Q+ N +P
Sbjct: 39 LIEEEVFYNPAQIINRDLTVLVI----------------------DTFQE-----NRQP- 70
Query: 286 ITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDA 465
ITIL+ALSA+GLRS+R+A+EI I ANDLS ++ I NI+ N VS ++ + DA
Sbjct: 71 ITILDALSASGLRSVRFAQEIQNVKEIYANDLSLASLALIDENIKLNNVSN-VKIYNMDA 129
Query: 466 CMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETC 645
LM + +F IDLDPYG FLDSA+ S D LL +T TD VL G + C
Sbjct: 130 NKLMKE----DIKFDVIDLDPYGTVCPFLDSAIHSCDD-SLLCITCTDSRVLCGPDTQKC 184
Query: 646 YCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
+ +YG K C E LR +L I Q A R + I P+
Sbjct: 185 FAQYGTARTKMNCFAENGLRTLLYTISQAAGRLGKAIKPL 224
>UniRef50_A1RX58 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;
n=2; cellular organisms|Rep: TRNA
(Guanine-N(2)-)-methyltransferase - Thermofilum pendens
(strain Hrk 5)
Length = 384
Score = 121 bits (292), Expect = 2e-26
Identities = 63/160 (39%), Positives = 99/160 (61%)
Frame = +1
Query: 286 ITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDA 465
+ +++A SATG+R IRYA E +I ND++ +AVE I+ N++ N VS I+E +DA
Sbjct: 59 LVVVDAFSATGIRGIRYALENEGVGRVILNDINPRAVEIIRQNVDLNGVSDIVEVRREDA 118
Query: 466 CMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETC 645
L+ +DLDP+G P+ ++D A++ ++ GLL VTATD+ L G P+TC
Sbjct: 119 SSLLASLSGVD----IVDLDPFGSPAEYVDPALRCLKHKGLLCVTATDLPPLLGKYPKTC 174
Query: 646 YCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
KY + S++T+ E+A+RI+L I + A + R IVP+
Sbjct: 175 VRKYFSKSIETEFSRELAVRILLYFIAREAAKLGRTIVPL 214
>UniRef50_Q2NI56 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=2;
Methanobacteriaceae|Rep: N(2),N(2)-dimethylguanosine
tRNA methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Methanosphaera
stadtmanae (strain DSM 3091)
Length = 390
Score = 119 bits (287), Expect = 7e-26
Identities = 72/212 (33%), Positives = 112/212 (52%)
Frame = +1
Query: 121 VFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITILE 300
VFYNPV E NRD+S+AV I Y+ E + I+I +
Sbjct: 27 VFYNPVMEMNRDISVAV----INQYRKEV-----------------------DHDISICD 59
Query: 301 ALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMY 480
A TG+R RY+KEI ++ D++ AVE K N++ N +S + E +DA +L+
Sbjct: 60 AFGGTGIRGARYSKEIHGVEKVVVGDVNPLAVEISKKNMQLNNISNV-EVQKNDANILLQ 118
Query: 481 KHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYG 660
+K F +D+DP+G P++F S +I+ GGL+ ++ATD + L G + C KYG
Sbjct: 119 SNKG---LFDVVDIDPFGTPAMFTQSTAANIRPGGLICISATDTSALCGTYHDPCLRKYG 175
Query: 661 AVSLKTKCCHEMALRIMLQCIEQHANRYSRYI 756
A LKT+ CHE +RI++ I ++ +Y+
Sbjct: 176 AKPLKTEYCHENGIRILIAFISRNLAVNQKYL 207
>UniRef50_Q9V1P3 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=6;
Thermococcaceae|Rep: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Pyrococcus abyssi
Length = 383
Score = 111 bits (267), Expect(2) = 4e-25
Identities = 70/170 (41%), Positives = 103/170 (60%), Gaps = 14/170 (8%)
Frame = +1
Query: 277 EPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNI---------EHN- 426
+P+I +L+ALSATG+R IR+A E P A I ND+SE A +K N+ E N
Sbjct: 51 KPRI-VLDALSATGIRGIRFALETP-AEEIWMNDISEDAYNLMKKNVLLNFKGELEESNG 108
Query: 427 ----QVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLL 594
+ + + +HDDA LM + KH + F IDLDP+G P FLD+A++S++ G+L
Sbjct: 109 RAVLKSEKTLVVNHDDANRLMAE-KH--RYFHFIDLDPFGSPMEFLDTALRSVKRKGILG 165
Query: 595 VTATDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRY 744
+TATD A L G P+ C KY AV L+ + CHE+ RI++ + ++A +Y
Sbjct: 166 ITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKY 215
Score = 26.2 bits (55), Expect(2) = 4e-25
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = +1
Query: 121 VFYNPVQEFNRDLSIAVLTL 180
VFYNP NRD+++ +L +
Sbjct: 30 VFYNPRMALNRDIAVVLLNV 49
>UniRef50_Q8ZWT5 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=4;
Pyrobaculum|Rep: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Pyrobaculum
aerophilum
Length = 363
Score = 109 bits (262), Expect(2) = 2e-24
Identities = 62/160 (38%), Positives = 93/160 (58%)
Frame = +1
Query: 286 ITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDA 465
+ + E LS TG+R IRYA E +I ND+S++AVE IK N+E N V E ++DA
Sbjct: 55 LVVCEPLSGTGVRGIRYAVESGVVGKLILNDISKEAVELIKKNLEINGVDA--EVYNEDA 112
Query: 466 CMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETC 645
+L++K K +D+DP+G P+ F+ A +++++ GL+ TATD AVL G P C
Sbjct: 113 NVLLHKLKDSCD---VVDIDPFGSPAPFIHGAFRALKEEGLICATATDTAVLVGRYPRKC 169
Query: 646 YCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
+YG+V +KT E+ LR +L I + A I P+
Sbjct: 170 LRRYGSVIVKTPFYIEVGLRNLLGYIARVAAAEDYKITPL 209
Score = 25.8 bits (54), Expect(2) = 2e-24
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = +1
Query: 121 VFYNPVQEFNRDLSIAVLTLF 183
VFYNP E NR LS +L +
Sbjct: 30 VFYNPAMEKNRTLSTLLLKAY 50
>UniRef50_A5UM08 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase, Trm1; n=1; Methanobrevibacter smithii
ATCC 35061|Rep: N2,N2-dimethylguanosine tRNA
methyltransferase, Trm1 - Methanobrevibacter smithii
(strain PS / ATCC 35061 / DSM 861)
Length = 388
Score = 111 bits (267), Expect = 2e-23
Identities = 80/236 (33%), Positives = 113/236 (47%), Gaps = 7/236 (2%)
Frame = +1
Query: 70 LKTIKEGQAEICL-------TTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKK 228
+KT++EG +I + VFYNP E NRD+SI L
Sbjct: 6 IKTVEEGLTKIQFPEFDKISSDAPVFYNPHMELNRDISILAL------------------ 47
Query: 229 QKKLETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIK 408
+T Q +E + I I + +G+R +RY EI ++ ND+SE A E +
Sbjct: 48 ----QTFQKQE-----DRNINICDLFGGSGIRGVRYKNEIDGVGHVFINDISETANEYER 98
Query: 409 HNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGL 588
HN+E N + + IE DA M + H+ F ID+DP+G PS FLDSA + L
Sbjct: 99 HNVELNNL-KDIEIFQHDASMFLRMHRG---EFDVIDIDPFGTPSPFLDSAGYCSRRNSL 154
Query: 589 LLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYI 756
L VTATD + L G E C KY A K++ CHE +RI+ + +YS+ I
Sbjct: 155 LCVTATDTSALCGTYKEPCIRKYNAKPYKSEYCHETGIRILAGFVALTLAKYSKSI 210
>UniRef50_Q386C5 Cluster: N(2), N(2)-dimethylguanosine tRNA
methyltransferase, putative; n=3; Trypanosoma|Rep: N(2),
N(2)-dimethylguanosine tRNA methyltransferase, putative
- Trypanosoma brucei
Length = 802
Score = 110 bits (264), Expect = 4e-23
Identities = 47/84 (55%), Positives = 64/84 (76%)
Frame = +1
Query: 514 IDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHE 693
+DLDPYG S FL+ AV+ I++GGLLLVT+TD A+L GN P+TC+ KY V K CHE
Sbjct: 344 VDLDPYGSASPFLEGAVRCIREGGLLLVTSTDSAILCGNYPDTCHAKYNTVPTKNAACHE 403
Query: 694 MALRIMLQCIEQHANRYSRYIVPI 765
MA+RI+L +E+ AN++ +YIVP+
Sbjct: 404 MAVRILLAAVERVANKHRKYIVPL 427
Score = 80.2 bits (189), Expect = 5e-14
Identities = 52/116 (44%), Positives = 64/116 (55%)
Frame = +1
Query: 115 EKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITI 294
+ VFYNP Q NRDLS+ V+ F +L + E K K G ITI
Sbjct: 163 QAVFYNPAQVVNRDLSVCVIACF------SQLRKEEPKNK-----------GGTRRGITI 205
Query: 295 LEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDD 462
LEALSATGLR+IRY KEIP IIAND+ AVE I+ N E N+V + T ++
Sbjct: 206 LEALSATGLRAIRYYKEIPDVRFIIANDIDCDAVECIRRNCEFNEVPCVAPTFQEN 261
>UniRef50_Q5KQ20 Cluster: TRNA (Guanine-N2-)-methyltransferase,
putative; n=1; Filobasidiella neoformans|Rep: TRNA
(Guanine-N2-)-methyltransferase, putative - Cryptococcus
neoformans (Filobasidiella neoformans)
Length = 666
Score = 108 bits (260), Expect = 1e-22
Identities = 51/112 (45%), Positives = 69/112 (61%), Gaps = 4/112 (3%)
Frame = +1
Query: 442 IETSHDDACMLMYKHKH---PSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDM 612
++ + DAC MY H+ PS R +DLDPYG + FLD+A+ I DGGLL +T TD+
Sbjct: 257 VKINEGDACAFMYNHRSAVGPSARVDVVDLDPYGTAAPFLDAAIGCISDGGLLAITCTDL 316
Query: 613 AVLAGNS-PETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
AVLAG PE CY YG ++ + HE ALR+++ + A RY RYI P+
Sbjct: 317 AVLAGQQYPEKCYSNYGGTNVHAEYTHEAALRLVMHSLASVAARYGRYITPL 368
Score = 70.1 bits (164), Expect = 6e-11
Identities = 44/98 (44%), Positives = 56/98 (57%), Gaps = 11/98 (11%)
Frame = +1
Query: 172 LTLFIEDYKAEKLARFEKKQKKLETVQD-EESGGNPE----------PKITILEALSATG 318
+T+ E+ KAEK E + L V++ EE P P I ILEAL+ATG
Sbjct: 97 VTIDAEEEKAEKEGTMEVEGNALAEVKEGEEPVAGPSNGAGVSKFRAPSINILEALAATG 156
Query: 319 LRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQV 432
LRSIRYAKEIP ++ANDLS A E ++ N+E N V
Sbjct: 157 LRSIRYAKEIPNVKYVLANDLSPSACEAMRRNVEFNGV 194
Score = 41.9 bits (94), Expect = 0.017
Identities = 26/67 (38%), Positives = 35/67 (52%)
Frame = +1
Query: 85 EGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEES 264
E I L E F NPVQ +NRD+S+AV+ + E K E E+K + ++ E
Sbjct: 24 ESTTTIFLPNEGAFLNPVQHYNRDMSVAVIRAWNEMRKEE----LEEKWR----IRLERR 75
Query: 265 GGNPEPK 285
GG P PK
Sbjct: 76 GGKPRPK 82
>UniRef50_Q58356 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=6;
Methanococcales|Rep: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Methanococcus
jannaschii
Length = 374
Score = 107 bits (257), Expect = 3e-22
Identities = 68/220 (30%), Positives = 118/220 (53%), Gaps = 3/220 (1%)
Frame = +1
Query: 115 EKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITI 294
++VFYNP + RD+SIAV+ F+ Y + K I
Sbjct: 22 DEVFYNPRMKTCRDISIAVIQAFLNLY-------------------------HKRDKFYI 56
Query: 295 LEALSATGLRSIRYAKEIPY--ATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDAC 468
+AL+ +G+R +RYAKE+ + + ND++ +A E I +N + N++ I + ++DA
Sbjct: 57 ADALAGSGIRGLRYAKELEFNGELKVFLNDINPKAYEKIINNAKLNEIENI-DVFNEDAN 115
Query: 469 MLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQS-IQDGGLLLVTATDMAVLAGNSPETC 645
+ KH + F +D+DP+G P+ +++ A+++ + GLL +TATD A L G S ++C
Sbjct: 116 TFLSKH---FRFFNVVDIDPFGSPAPYVEQAIRALVTRNGLLCLTATDTAALCGRSKKSC 172
Query: 646 YCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
KY A L + CHE ALR+++ + + A +Y + P+
Sbjct: 173 LRKYLAYPLFGRDCHEFALRVLVGYVMRMATKYELALKPV 212
>UniRef50_Q97ZH0 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=2;
Sulfolobus|Rep: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Sulfolobus
solfataricus
Length = 378
Score = 92.3 bits (219), Expect(2) = 7e-21
Identities = 55/163 (33%), Positives = 93/163 (57%)
Frame = +1
Query: 277 EPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSH 456
+PKI I++ALSATG+R IRY E + +I ND + A I+ N ++N + + +
Sbjct: 55 KPKI-IVDALSATGIRGIRYYVESWKSEQLILNDKNPNATSLIQINAKNNGIENA-KIYN 112
Query: 457 DDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSP 636
DA L+Y+ K ID+DP+G P+ F+ S++ + G++ TATD++ L G+SP
Sbjct: 113 KDANALLYEVKSDY-----IDIDPFGSPAPFILSSLNAATRNGIVAFTATDLSPLEGSSP 167
Query: 637 ETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
+C KY A++ K E+ LR+++ I + A + + P+
Sbjct: 168 TSCRRKYDAINHKLSSSKELGLRVLIGKIIREAAILEKTVYPL 210
Score = 31.5 bits (68), Expect(2) = 7e-21
Identities = 12/20 (60%), Positives = 16/20 (80%)
Frame = +1
Query: 121 VFYNPVQEFNRDLSIAVLTL 180
VFYNP FNRDLS+ V+++
Sbjct: 34 VFYNPKMTFNRDLSVIVVSI 53
>UniRef50_O29443 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=1; Archaeoglobus
fulgidus|Rep: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Archaeoglobus
fulgidus
Length = 349
Score = 94.7 bits (225), Expect(2) = 7e-21
Identities = 58/156 (37%), Positives = 91/156 (58%)
Frame = +1
Query: 298 EALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLM 477
+ALSA+G+R IR A E + ND+S +AV+ I+ N+ N VS E + DA +M
Sbjct: 42 DALSASGIRGIRAALEA--GKKAVFNDVSPKAVKVIEENLRENGVSG--EVINGDAAAVM 97
Query: 478 YKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKY 657
+ + F ID+DP+G P+ F+DSA S + L VTATD A L G++ + KY
Sbjct: 98 RQ-----RAFEHIDIDPFGSPAPFMDSACFSAKR--YLSVTATDTAALCGSATNSGLKKY 150
Query: 658 GAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
GA ++KT HE+ LR+++ + + A +Y + + P+
Sbjct: 151 GAFAVKTDVYHEVGLRMLIGFVVREATKYEKALFPL 186
Score = 29.1 bits (62), Expect(2) = 7e-21
Identities = 16/40 (40%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = +1
Query: 79 IKEGQAEICLTTEKVFYNPVQEFNRDLSIAVL-TLFIEDY 195
++EG+A + E VFYNP F RDL + V T+ ++Y
Sbjct: 3 VEEGRARV--KVEGVFYNPRMRFCRDLDMLVFATMDSKEY 40
>UniRef50_O67010 Cluster: Probable N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=1; Aquifex
aeolicus|Rep: Probable N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Aquifex aeolicus
Length = 392
Score = 101 bits (241), Expect = 3e-20
Identities = 70/215 (32%), Positives = 109/215 (50%)
Frame = +1
Query: 121 VFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITILE 300
VFYNP NRDL AVL L +Y +KL R + + +
Sbjct: 26 VFYNPRMRVNRDL--AVLGL---EYLCKKLGR----------------------PVKVAD 58
Query: 301 ALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMY 480
LSA+G+R+IR+ E AND+S +A+E +K N + N + H
Sbjct: 59 PLSASGIRAIRFLLETSCVEKAYANDISSKAIEIMKENFKLNNIPEDRYEIHGMEANFFL 118
Query: 481 KHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYG 660
+ K F +DLDP+G P F++S S++ GG+L +TATD A L+G P+TC +Y
Sbjct: 119 R-KEWGFGFDYVDLDPFGTPVPFIESVALSMKRGGILSLTATDTAPLSGTYPKTCMRRYM 177
Query: 661 AVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
A L+ + HE+ +RI+++ + + A +Y ++PI
Sbjct: 178 ARPLRNEFKHEVGIRILIKKVIELAAQYDIAMIPI 212
>UniRef50_Q0W1J5 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase; n=1; uncultured methanogenic archaeon
RC-I|Rep: N(2),N(2)-dimethylguanosine tRNA
methyltransferase - Uncultured methanogenic archaeon
RC-I
Length = 365
Score = 85.0 bits (201), Expect(2) = 5e-20
Identities = 51/159 (32%), Positives = 88/159 (55%)
Frame = +1
Query: 289 TILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDAC 468
T ++A++A+G+R IR KE+P ++ ND A E +K N E N V+ + ++ A
Sbjct: 42 TYVDAMAASGIRGIRVKKEVPREIDVTINDWDAGAYELLKRNAEANGVT--VNATNRGAN 99
Query: 469 MLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCY 648
L+ S ++ +D+DP+G PS ++DS +S + + VTATD A L G +
Sbjct: 100 TLL-----SSTQYDFVDIDPFGTPSPYIDSVCRSAK--RFMGVTATDTAPLCGAHLRSGM 152
Query: 649 CKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
KYGA +KT+ E+ LR+++ + + +Y R + P+
Sbjct: 153 RKYGAFPVKTEYYPEIGLRVLMGKVVREQAKYDRAVRPL 191
Score = 35.9 bits (79), Expect(2) = 5e-20
Identities = 18/36 (50%), Positives = 24/36 (66%)
Frame = +1
Query: 70 LKTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLT 177
+ I+EG+ I + E VFYNP E NRDL++A LT
Sbjct: 1 MAVIREGKVAIEMG-EGVFYNPRMEMNRDLNVACLT 35
>UniRef50_Q7QW00 Cluster: GLP_239_44022_45620; n=1; Giardia lamblia
ATCC 50803|Rep: GLP_239_44022_45620 - Giardia lamblia
ATCC 50803
Length = 532
Score = 99 bits (238), Expect = 6e-20
Identities = 75/236 (31%), Positives = 113/236 (47%), Gaps = 6/236 (2%)
Frame = +1
Query: 76 TIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQD 255
T EG +VF+NP Q FNRD+S+ V+ +++ + TVQ
Sbjct: 20 THTEGTVSFTYNPSQVFFNPTQIFNRDISLLVVDQYLQHIHDQS------------TVQ- 66
Query: 256 EESGGNPEPKITILEALSATGLRSIRYAKEIPYAT--NIIANDLSEQAVETIKHNIEHNQ 429
K I++ LSA+GLR++RY++E+ +IIA D+S A I N E N
Sbjct: 67 ---------KAIIIDCLSASGLRALRYSRELHVTVPLHIIAVDISPAATADIAMNHEANP 117
Query: 430 VSR---IIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVT 600
V+ E S DA + H A ID+DPYG P+ FL +A+ + GLL +T
Sbjct: 118 VTLPDVAFEISCMDANRKLQDLHHSRMPAAVIDIDPYGHPTPFLPAALDACSHNGLLCIT 177
Query: 601 ATDMAVLAGNSPETCYCKYGAVSLKTKC-CHEMALRIMLQCIEQHANRYSRYIVPI 765
ATD A+ + C+ +Y A + HE ++RI++ I + A R I P+
Sbjct: 178 ATDGAITCRKQCKECFIRYDAAPATGRVWAHEGSIRIVMGAIAKEAARRRASITPL 233
>UniRef50_Q4QG60 Cluster: N(2), N(2)-dimethylguanosine tRNA
methyltransferase, putative; n=3; Leishmania|Rep: N(2),
N(2)-dimethylguanosine tRNA methyltransferase, putative
- Leishmania major
Length = 718
Score = 99.5 bits (237), Expect = 8e-20
Identities = 42/84 (50%), Positives = 61/84 (72%)
Frame = +1
Query: 514 IDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHE 693
+DLDPYG S FLD A + I++GGL+LVT+TD A+L GN +T + KY ++ K CHE
Sbjct: 252 VDLDPYGSASPFLDGAFRCIKEGGLMLVTSTDSAILCGNFADTAHAKYSSMPYKAAHCHE 311
Query: 694 MALRIMLQCIEQHANRYSRYIVPI 765
A+R +L C+E+ ANR+ ++IVP+
Sbjct: 312 AAVRTLLACVERVANRHQKFIVPL 335
Score = 76.2 bits (179), Expect = 8e-13
Identities = 50/118 (42%), Positives = 64/118 (54%)
Frame = +1
Query: 79 IKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDE 258
+ + +A+ L + VFYNP Q NRDLSI+V+ +F +L +
Sbjct: 53 LSDEEADNQLVDQAVFYNPAQVVNRDLSISVIEVF----------------SRLRLTEPR 96
Query: 259 ESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQV 432
GG E ITILEALSATGLR+IRY KEI IIAND+ AV+ I N +N V
Sbjct: 97 RRGGTNEG-ITILEALSATGLRAIRYYKEITNVRYIIANDMDADAVDCIVRNCAYNGV 153
>UniRef50_A7DP78 Cluster: tRNA (Guanine-N(2)-)-methyltransferase;
n=1; Candidatus Nitrosopumilus maritimus SCM1|Rep: tRNA
(Guanine-N(2)-)-methyltransferase - Candidatus
Nitrosopumilus maritimus SCM1
Length = 382
Score = 98.7 bits (235), Expect = 1e-19
Identities = 51/162 (31%), Positives = 88/162 (54%)
Frame = +1
Query: 280 PKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHD 459
PKI LE LS G R +R A E+ N++ NDL+ A++ +++ + N++ + E
Sbjct: 62 PKI-FLEGLSGIGARGLRVANELKI-DNLVINDLNPTALKMAEYSAKLNEIKNV-EFLEK 118
Query: 460 DACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPE 639
+ C +R + +D+DP+G P+ F D +++ GG+L V ATD+ VL G
Sbjct: 119 EVCRFFSNFSKKGERGSIVDIDPFGSPAAFFDCGIRATMHGGILSVAATDLQVLNGLFQS 178
Query: 640 TCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
C KYG V ++ + +E+A+R++L C+ A R + P+
Sbjct: 179 ACKRKYGGVPVRAEYGNEIAIRLVLGCLRMVAARLGVEVEPM 220
>UniRef50_A4YHH9 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;
n=1; Metallosphaera sedula DSM 5348|Rep: TRNA
(Guanine-N(2)-)-methyltransferase - Metallosphaera
sedula DSM 5348
Length = 382
Score = 91.1 bits (216), Expect(2) = 2e-19
Identities = 57/159 (35%), Positives = 89/159 (55%)
Frame = +1
Query: 289 TILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDAC 468
++L+A+SATG+R IRY KE ++ ND + +VE I N+E N ++ + S DA
Sbjct: 58 SVLDAMSATGVRGIRYVKESGVKGEVLFNDKNPVSVELISKNLELNGITGKVLRS--DAN 115
Query: 469 MLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCY 648
LM++ K DLDP+G P+ +L SA+ S++ G+L VTATD++ L G S +
Sbjct: 116 SLMHQVK-----VGYTDLDPFGSPAPYLFSAISSLRRKGVLGVTATDLSALEGKSRTSSK 170
Query: 649 CKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
KYG + E LR++L I + A+ + I P+
Sbjct: 171 RKYGVQGSRLSYSKEAGLRVLLGKIVKEASVQEKGIRPL 209
Score = 27.5 bits (58), Expect(2) = 2e-19
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = +1
Query: 121 VFYNPVQEFNRDLSIAVLTL 180
VFYNP FNRD+S+ +++
Sbjct: 34 VFYNPRMVFNRDVSVLAVSV 53
>UniRef50_UPI00015BCD4C Cluster: UPI00015BCD4C related cluster; n=1;
unknown|Rep: UPI00015BCD4C UniRef100 entry - unknown
Length = 366
Score = 97.1 bits (231), Expect = 4e-19
Identities = 54/161 (33%), Positives = 94/161 (58%)
Frame = +1
Query: 283 KITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDD 462
K+ I + +SA+G+R +R KE ND+ ++A+E + N+E Q +E + D
Sbjct: 52 KLFIADPMSASGVRVLRLLKETNVVEKAFLNDIKKEAIELAQKNLEGYQN---VEYFNKD 108
Query: 463 ACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPET 642
A + + ++K F ID+DPYG P FL+SA+ +++ GGL+ +TATD A L+G+ P
Sbjct: 109 ARIFLLENKD----FDYIDIDPYGSPIGFLESAINALKTGGLIGITATDTASLSGSYPFK 164
Query: 643 CYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
+ +Y A L ++ HE ALRI+++ + + + R + P+
Sbjct: 165 AFRRYSAKPLDSEFYHESALRILVKSVIEASFRLDVVLKPV 205
>UniRef50_A4RKM5 Cluster: Putative uncharacterized protein; n=1;
Magnaporthe grisea|Rep: Putative uncharacterized protein
- Magnaporthe grisea (Rice blast fungus) (Pyricularia
grisea)
Length = 702
Score = 96.7 bits (230), Expect = 6e-19
Identities = 65/194 (33%), Positives = 103/194 (53%), Gaps = 27/194 (13%)
Frame = +1
Query: 187 EDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNI 366
++ +AE A + Q K+ + + P++ IL+ALSATGLR++RYA E+P+ T +
Sbjct: 166 QEAQAESAAPADVTQDKISETP-VKLPNDKTPRLKILDALSATGLRALRYAHELPFPTMV 224
Query: 367 IANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMY-----------------KHKHP 495
+NDL AV++IK N++HN++ I +H +A MY KH+
Sbjct: 225 TSNDLLAAAVDSIKVNVKHNKLEDSIRVNHGNAQGHMYSVHADELTRSMLAPSGNKHRRQ 284
Query: 496 SK---------RFAAIDLDPYGCPSIFLDSAVQSIQ-DGGLLLVTATDMAVLAGNSPETC 645
+ ++ IDLDPYG + F DSAVQ+++ DGGLL VT TD LA +
Sbjct: 285 TPQEQLNNLGGKYDVIDLDPYGTAATFFDSAVQAVRDDGGLLCVTCTDPGPLATVWRSSR 344
Query: 646 YCKYGAVSLKTKCC 687
C+ + +++ C
Sbjct: 345 CCRSRSTTMRAFLC 358
Score = 39.5 bits (88), Expect = 0.089
Identities = 22/63 (34%), Positives = 36/63 (57%), Gaps = 8/63 (12%)
Frame = +1
Query: 115 EKVFYNPVQEFNRDLSIAVLTLF--IEDYKAEKLAR------FEKKQKKLETVQDEESGG 270
+ VFYNP+Q+FNRDLS+ + + + K +LA+ +KK+K+ E + E G
Sbjct: 69 QTVFYNPIQQFNRDLSVLAIKAYGKVVMEKRMRLAQRRMEMFADKKRKRQEKREHREEGE 128
Query: 271 NPE 279
P+
Sbjct: 129 RPQ 131
>UniRef50_Q8PU28 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=5;
Methanosarcinaceae|Rep: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Methanosarcina
mazei (Methanosarcina frisia)
Length = 388
Score = 96.7 bits (230), Expect = 6e-19
Identities = 67/215 (31%), Positives = 114/215 (53%)
Frame = +1
Query: 121 VFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITILE 300
VFYNP E NRD+++A F+E ++K + +++E I ++
Sbjct: 31 VFYNPEMELNRDINVAATAAFVERLLSKK-----------DILREE---------IRYVD 70
Query: 301 ALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMY 480
A SA+G+R +R A E+ + + ND S +A E IK NI+ N + + + +A +L++
Sbjct: 71 AFSASGIRGLRIAGEVGIHSTM--NDWSHEAFELIKENIKINGLEEKAQATRRNANVLLH 128
Query: 481 KHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYG 660
+ +RF +D+DP+G PS +LD+A S +L VTATD A L G + KY
Sbjct: 129 E-----QRFHIVDVDPFGTPSPYLDAAASSAYS--MLSVTATDTAPLCGAHLNSGIRKYA 181
Query: 661 AVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
+V L T+ EM LR++L + ++ + ++P+
Sbjct: 182 SVPLNTEYHSEMGLRVLLGACARELAKHEKGMLPL 216
>UniRef50_A3HAS8 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;
n=1; Caldivirga maquilingensis IC-167|Rep: TRNA
(Guanine-N(2)-)-methyltransferase - Caldivirga
maquilingensis IC-167
Length = 380
Score = 91.1 bits (216), Expect(2) = 1e-18
Identities = 56/148 (37%), Positives = 81/148 (54%)
Frame = +1
Query: 292 ILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACM 471
+ E + TG+RSIRY KE +IA D+ + AV N+E N + I+ + DA
Sbjct: 63 LCEPFTGTGVRSIRYVKEAG-VEKVIAGDIDDAAVNLASRNVELNGLRDQIKVTRSDANA 121
Query: 472 LMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYC 651
L+ +K ID+DPYG P+ F++SA+ SI+ GGLL TATD+AVL G+
Sbjct: 122 LLANNKCD-----IIDVDPYGSPAPFINSALLSIKHGGLLCATATDLAVLQGSYVNKAIR 176
Query: 652 KYGAVSLKTKCCHEMALRIMLQCIEQHA 735
+YG L+ E+ LR +L I + A
Sbjct: 177 RYGFRPLRGFLSREIGLRGLLGFIARQA 204
Score = 25.0 bits (52), Expect(2) = 1e-18
Identities = 8/22 (36%), Positives = 14/22 (63%)
Frame = +1
Query: 121 VFYNPVQEFNRDLSIAVLTLFI 186
VFYNP NR +S+ ++ ++
Sbjct: 33 VFYNPAMANNRSISVIIVRAYL 54
>UniRef50_A0RV27 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase; n=1; Cenarchaeum symbiosum|Rep:
N2,N2-dimethylguanosine tRNA methyltransferase -
Cenarchaeum symbiosum
Length = 377
Score = 95.5 bits (227), Expect = 1e-18
Identities = 50/158 (31%), Positives = 85/158 (53%)
Frame = +1
Query: 292 ILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACM 471
+L+ +S G R +R A E+ + ND +EQA+ + + + N + + TS+ +AC
Sbjct: 65 LLDCMSGLGARGLRAANEVG-GIEVCLNDTNEQALNIARESAKLNCLD-CVSTSNAEACS 122
Query: 472 LMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYC 651
+ +H KR A +D+DP+G P+ LD ++++ GG+L +TATD+ VL G P+ C
Sbjct: 123 FLAEHWGRGKRAAIVDIDPFGSPAPHLDCCLRAVSHGGMLSITATDLQVLNGIFPDACRR 182
Query: 652 KYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
YG + E A+R++L C+ A R P+
Sbjct: 183 IYGGSPARAVFGAEAAVRLLLGCLRNVAARLGVSFEPL 220
>UniRef50_UPI00015BB118 Cluster: tRNA
(guanine-N(2)-)-methyltransferase; n=1; Ignicoccus
hospitalis KIN4/I|Rep: tRNA
(guanine-N(2)-)-methyltransferase - Ignicoccus
hospitalis KIN4/I
Length = 386
Score = 93.5 bits (222), Expect = 5e-18
Identities = 48/147 (32%), Positives = 86/147 (58%)
Frame = +1
Query: 283 KITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDD 462
++ +LE L+ +G+RS+RYA E A ++A D +AVE IK+NI N++ ++ +
Sbjct: 66 RLEVLEPLAGSGVRSLRYAVEAG-ADKVLAVDADPKAVELIKYNIRLNKLEDKVKVLLAE 124
Query: 463 ACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPET 642
A +++ + + +D+DP+G P F D A+++ + GL+ TATD A L G P++
Sbjct: 125 ANAVLHSRELRGGYYDLVDVDPFGSPMPFADGAIRAARRRGLVAFTATDTAPLTGARPQS 184
Query: 643 CYCKYGAVSLKTKCCHEMALRIMLQCI 723
KYGA +T E+A+R+++ +
Sbjct: 185 ALRKYGASVRRTPFSKEVAIRVLIAAL 211
>UniRef50_A7AUB1 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase, putative; n=1; Babesia bovis|Rep:
N(2),N(2)-dimethylguanosine tRNA methyltransferase,
putative - Babesia bovis
Length = 544
Score = 92.3 bits (219), Expect = 1e-17
Identities = 39/84 (46%), Positives = 58/84 (69%)
Frame = +1
Query: 514 IDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHE 693
ID+DPY ++FLDSA++ ++ GG+L VT+TDM L GN+P + KYG S+K CHE
Sbjct: 203 IDVDPYSTATVFLDSAIRCVKSGGMLFVTSTDMPTLCGNNPLVSFYKYGGSSIKATFCHE 262
Query: 694 MALRIMLQCIEQHANRYSRYIVPI 765
++LRI+L I A++Y R + P+
Sbjct: 263 LSLRILLYTIATTASKYQRVMEPM 286
Score = 65.7 bits (153), Expect = 1e-09
Identities = 43/124 (34%), Positives = 65/124 (52%), Gaps = 4/124 (3%)
Frame = +1
Query: 76 TIKEGQ---AEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLET 246
T+ EG + + + + +FYNP Q FNRDLS+ VL F+E Y+ ++ A
Sbjct: 6 TVTEGLVTVSSLSVDGQPLFYNPPQVFNRDLSLIVLKTFVE-YEKDRAATLVSSNGN-SV 63
Query: 247 VQDEESGGNPEPKITILEALSATGLRSIRYAKEI-PYATNIIANDLSEQAVETIKHNIEH 423
V+D + +LE L+ATG+RSIRY KE+ Y ++ NDL + I N +
Sbjct: 64 VKDFVG-------VNVLEMLAATGIRSIRYLKELGDYVNHVYINDLDYNSASAIAINCRN 116
Query: 424 NQVS 435
N +S
Sbjct: 117 NGIS 120
>UniRef50_Q8TYY7 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase; n=1; Methanopyrus kandleri|Rep:
N2,N2-dimethylguanosine tRNA methyltransferase -
Methanopyrus kandleri
Length = 383
Score = 91.9 bits (218), Expect = 2e-17
Identities = 52/161 (32%), Positives = 91/161 (56%)
Frame = +1
Query: 280 PKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHD 459
PKI + + L+ G R IR A E+ ++ NDL+ +AVE I+ N+ N V + +
Sbjct: 50 PKI-VCDPLAGVGARGIRIAVELSPEV-VVLNDLNPRAVELIEENVRLNDVEDVCRIENR 107
Query: 460 DACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPE 639
DA LM++ + RF +D+DP+G P FLD+AV+++++ G++ ++ATD++ LAG P
Sbjct: 108 DANALMHEDELAG-RFDYVDIDPFGPPVPFLDAAVRTVRNRGVVGISATDVSALAGRYPR 166
Query: 640 TCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVP 762
+ KY + + E+A+R ++ I + +Y P
Sbjct: 167 SARRKYWVEVERVEFYQEVAIRALISYIVRTCAKYDLAFEP 207
>UniRef50_Q4N4W5 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase, putative; n=2; Theileria|Rep:
N2,N2-dimethylguanosine tRNA methyltransferase, putative
- Theileria parva
Length = 599
Score = 90.6 bits (215), Expect = 4e-17
Identities = 38/84 (45%), Positives = 58/84 (69%)
Frame = +1
Query: 514 IDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHE 693
IDLDPY + ++DSAV+ ++ GG+LL+T+TDM L GN+P + KYG S K+ CHE
Sbjct: 227 IDLDPYSSVTSYVDSAVRCVRSGGMLLITSTDMPTLCGNNPLVSFYKYGGTSFKSPFCHE 286
Query: 694 MALRIMLQCIEQHANRYSRYIVPI 765
++LR++L + A++Y R I P+
Sbjct: 287 LSLRVLLYSVMLTASKYKRVIEPL 310
Score = 58.4 bits (135), Expect = 2e-07
Identities = 41/107 (38%), Positives = 59/107 (55%), Gaps = 1/107 (0%)
Frame = +1
Query: 121 VFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITILE 300
+FYNP Q FNRDLS+ VL FI +++KK+E+ D + G + ILE
Sbjct: 43 LFYNPPQVFNRDLSLLVLKTFI-----------LQEKKKIES--DPKLGN--FIGVNILE 87
Query: 301 ALSATGLRSIRYAKEI-PYATNIIANDLSEQAVETIKHNIEHNQVSR 438
+L+ATG+R IRY +E+ P + NDL + E I N+ N + R
Sbjct: 88 SLAATGIRGIRYLRELGPLVGMVTFNDLDRNSAEMILKNLTLNSMPR 134
>UniRef50_A2SRK9 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;
n=3; Methanomicrobiales|Rep: TRNA
(Guanine-N(2)-)-methyltransferase - Methanocorpusculum
labreanum (strain ATCC 43576 / DSM 4855 / Z)
Length = 379
Score = 82.2 bits (194), Expect(2) = 8e-17
Identities = 59/157 (37%), Positives = 86/157 (54%)
Frame = +1
Query: 295 LEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACML 474
L+A++ATG+R +R A E I ND EQAV+ IK+N E ++ I + DDA L
Sbjct: 57 LDAMAATGVRGLRIANEAKIPVTI--NDRDEQAVKIIKYNAE--KLGGDISVTCDDANRL 112
Query: 475 MYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCK 654
M + RF +IDLDP+G P FLD+A ++ + L VTATD A L G + +
Sbjct: 113 MC-----TSRFDSIDLDPFGTPVPFLDAASRAAKH--YLFVTATDTAPLCGAHFKAGCRR 165
Query: 655 YGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
Y A T+ E+ LR+M+ + + +Y R + PI
Sbjct: 166 YFATPRNTEYHAEVGLRMMMGTMARELVKYDRGMKPI 202
Score = 27.9 bits (59), Expect(2) = 8e-17
Identities = 14/27 (51%), Positives = 19/27 (70%), Gaps = 2/27 (7%)
Frame = +1
Query: 121 VFYNPVQEFNRDLSIAVLTLFI--EDY 195
VFYN EFNRD+++ +LT + EDY
Sbjct: 31 VFYNTKMEFNRDMTV-LLTKIVQPEDY 56
>UniRef50_Q4J947 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase; n=1; Sulfolobus acidocaldarius|Rep:
N(2),N(2)-dimethylguanosine tRNA methyltransferase -
Sulfolobus acidocaldarius
Length = 382
Score = 82.6 bits (195), Expect(2) = 3e-16
Identities = 50/163 (30%), Positives = 86/163 (52%)
Frame = +1
Query: 277 EPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSH 456
+PK ++++ LSATG+R IRY EI II ND+ AVE IK N++ N + + +
Sbjct: 55 KPK-SLIDGLSATGVRGIRYGLEINGVEEIILNDIDSDAVELIKKNVKINDLESRAKIYN 113
Query: 457 DDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSP 636
++ L+++ K +D+DP+G P+ FL S+ + + + +TATD+A L +S
Sbjct: 114 NNINSLLHEIK-----VDYVDIDPFGSPAPFLLSSFSAAKSKQYVAITATDLAALMCSSK 168
Query: 637 ETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
+ KYG + K E+ LR ++ A + + P+
Sbjct: 169 TSARRKYGLICNKMSFSRELGLRGLISKAITEAAVVEKAVTPV 211
Score = 25.4 bits (53), Expect(2) = 3e-16
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = +1
Query: 121 VFYNPVQEFNRDLSI 165
VFYNP NRDLS+
Sbjct: 34 VFYNPKMILNRDLSV 48
>UniRef50_Q64CS6 Cluster: N(2)N(2)-dimethylguanosine tRNA
methyltransferase; n=3; environmental samples|Rep:
N(2)N(2)-dimethylguanosine tRNA methyltransferase -
uncultured archaeon GZfos1C11
Length = 371
Score = 87.4 bits (207), Expect = 3e-16
Identities = 59/157 (37%), Positives = 87/157 (55%)
Frame = +1
Query: 295 LEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACML 474
L+AL+ +G+R +R A E+ +I ND S A E IK NI N + +++A +L
Sbjct: 56 LDALAGSGVRGVRIANEVGMGVHI--NDRSTPAYELIKRNIALNSLEERARAYNENANIL 113
Query: 475 MYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCK 654
+ + R+ +DLDP+G P FLD+A +S++ LLLVTATD A L G + K
Sbjct: 114 LLQ-----TRYDMVDLDPFGSPVPFLDAACKSVK--RLLLVTATDTAPLCG-AHTGGMRK 165
Query: 655 YGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
YGA L T EMA RI+L + + +Y + I P+
Sbjct: 166 YGAKPLNTDYHTEMATRILLGAVTRDLCKYDKAIQPL 202
>UniRef50_A2BK15 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase; n=1; Hyperthermus butylicus DSM
5456|Rep: N(2),N(2)-dimethylguanosine tRNA
methyltransferase - Hyperthermus butylicus (strain DSM
5456 / JCM 9403)
Length = 396
Score = 87.4 bits (207), Expect = 3e-16
Identities = 50/151 (33%), Positives = 81/151 (53%)
Frame = +1
Query: 283 KITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDD 462
K+ ++E L+ +G+R++RYA E + A+D+ AV + N E N+VS + D
Sbjct: 67 KLVVVEPLAGSGVRAVRYAVEA--GAIVFASDIDSDAVYLSRVNAERNKVSERVRVEKAD 124
Query: 463 ACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPET 642
A M + + ID+DP+G P+ FLD+A+Q+++ G+L VTATD A L+G P
Sbjct: 125 ANEFMARLPRMGVKPTIIDIDPFGSPAPFLDTAIQALRPRGVLAVTATDTAPLSGTHPRA 184
Query: 643 CYCKYGAVSLKTKCCHEMALRIMLQCIEQHA 735
+Y + E A+RI+ I + A
Sbjct: 185 LRRRYDVRPGRLAWEKEQAVRILAGYIIRRA 215
>UniRef50_Q8SR99 Cluster: N2,N2-DIMETHYLGUANOSINE tRNA
METHYLTRANSFERASE; n=1; Encephalitozoon cuniculi|Rep:
N2,N2-DIMETHYLGUANOSINE tRNA METHYLTRANSFERASE -
Encephalitozoon cuniculi
Length = 432
Score = 86.6 bits (205), Expect = 6e-16
Identities = 72/245 (29%), Positives = 120/245 (48%), Gaps = 14/245 (5%)
Frame = +1
Query: 73 KTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQ 252
+TI E ++ + E F+NP Q+FNRD+SI V+ E +
Sbjct: 9 ETISE-ESTVITKNEWTFFNPAQKFNRDISIEVVK---------------------ECFK 46
Query: 253 DEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQV 432
D S I +L+A+SATGLR IRY KEI + + ND+S+ +V+TI+ N+ N +
Sbjct: 47 DRAS-------IRVLDAMSATGLRGIRYLKEIGNSI-VYLNDISQSSVDTIRSNVLLNGI 98
Query: 433 SRI---------IETSHDDACMLMYKHKHPSKR-----FAAIDLDPYGCPSIFLDSAVQS 570
I+ S + ++ + F ID+DP+G S ++DSA+++
Sbjct: 99 EDAEYFGSDLQGIKESGNARANVVKSDCNVLMTSLPCFFDVIDIDPFGSCSEYIDSALRA 158
Query: 571 IQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSR 750
I+ G+L +TATD VL N C KY +K +E+ LR ++ + + A+++
Sbjct: 159 IRHKGILCLTATDKGVLCSNE-RKCLIKYSTSIMKGMGMNEVPLRTIVSLVSRQASKFDC 217
Query: 751 YIVPI 765
+ P+
Sbjct: 218 SVEPV 222
>UniRef50_A0B927 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;
n=1; Methanosaeta thermophila PT|Rep: TRNA
(Guanine-N(2)-)-methyltransferase - Methanosaeta
thermophila (strain DSM 6194 / PT)
(Methanothrixthermophila (strain DSM 6194 / PT))
Length = 350
Score = 85.8 bits (203), Expect = 1e-15
Identities = 57/157 (36%), Positives = 84/157 (53%)
Frame = +1
Query: 295 LEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACML 474
L+A SA+G+R IR KE ++ ND+S A I+ N+ N +S E + + A L
Sbjct: 40 LDAFSASGIRGIRVRKEAG-VERVVMNDISPSACRRIRENLALNDISDC-EVTCESASAL 97
Query: 475 MYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCK 654
M + +RF AIDLDP+G P+ FL A S + L +TATD A L G ++ K
Sbjct: 98 MSR-----RRFEAIDLDPFGSPAQFLAPAASSAR--SYLFITATDTAPLCGAHLKSGVRK 150
Query: 655 YGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
Y AV L T+ EM +RI++ + + R R +P+
Sbjct: 151 YLAVPLNTEYHREMGVRILMGAVIREMARVDRRGIPL 187
>UniRef50_Q5CQU6 Cluster: Trm1p. N2,N2-dimethylguanosine tRNA
methyltransferase; n=2; Cryptosporidium|Rep: Trm1p.
N2,N2-dimethylguanosine tRNA methyltransferase -
Cryptosporidium parvum Iowa II
Length = 637
Score = 83.4 bits (197), Expect = 6e-15
Identities = 38/87 (43%), Positives = 54/87 (62%)
Frame = +1
Query: 505 FAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKC 684
F +D+DPYG + F+D V + ++ GL TATDM VL GN PE + KYG +LK
Sbjct: 219 FTIVDIDPYGTCAPFIDGTVHACEEDGLACFTATDMPVLCGNVPEVTFYKYGGNALKKSY 278
Query: 685 CHEMALRIMLQCIEQHANRYSRYIVPI 765
HEM+LR++L I A +Y + I+P+
Sbjct: 279 GHEMSLRLLLNTIITTAAKYQKSIIPL 305
Score = 64.5 bits (150), Expect = 3e-09
Identities = 45/145 (31%), Positives = 74/145 (51%), Gaps = 22/145 (15%)
Frame = +1
Query: 64 SNLKTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIE------DYKAEKLARF-- 219
S+ + I EG+ +I T + +FYNP Q FNRD+S+ V+ F+ K K F
Sbjct: 15 SSSEYIHEGKVKIHKTNDDIFYNPAQVFNRDISLIVIKSFLALRRKNLSEKYFKRLNFAI 74
Query: 220 ----EKKQKKLETVQDEESG---------GNPEPKITILEALSATGLRSIRYAKEIPY-A 357
+K ++ ++ +G G+ +T+LE L A+GLRSIRY KE+ +
Sbjct: 75 KGEGDKNNEQNNFTSNDLNGNTSIVSKEDGSSRFSVTVLEPLGASGLRSIRYIKELSHEI 134
Query: 358 TNIIANDLSEQAVETIKHNIEHNQV 432
+++ D+ AVE ++ N E N +
Sbjct: 135 DHVVCGDIDPVAVERMQQNFELNSI 159
>UniRef50_A7D179 Cluster: tRNA (Guanine-N(2)-)-methyltransferase;
n=1; Halorubrum lacusprofundi ATCC 49239|Rep: tRNA
(Guanine-N(2)-)-methyltransferase - Halorubrum
lacusprofundi ATCC 49239
Length = 433
Score = 82.6 bits (195), Expect = 1e-14
Identities = 71/215 (33%), Positives = 103/215 (47%)
Frame = +1
Query: 121 VFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITILE 300
VF+NP QE NRD+++AV L +D E + + L+
Sbjct: 79 VFFNPTQELNRDVTVAV----------------------LRAYRDREPRAS-----SYLD 111
Query: 301 ALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMY 480
A++A+G+R +R A E Y T D+ AVE N+ N + ET H D L+Y
Sbjct: 112 AMAASGIRGVRAAAE-GYDTTCA--DVDPDAVELAAENLAANDLDG--ETVHRDVNALLY 166
Query: 481 KHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYG 660
++ F +DLDPYG P F D+A+ + ++ L+ VTATD A L G + KYG
Sbjct: 167 ENV-----FDVVDLDPYGTPIPFADAALANARN--LVCVTATDTAPLCGAHLNSGIRKYG 219
Query: 661 AVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
AV T EM LR ++ + + A RY + PI
Sbjct: 220 AVPRNTDYHPEMGLRTLISALVRTAARYDKAARPI 254
>UniRef50_P57706 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=2;
Thermoplasma|Rep: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Thermoplasma
acidophilum
Length = 344
Score = 82.2 bits (194), Expect = 1e-14
Identities = 49/159 (30%), Positives = 85/159 (53%), Gaps = 1/159 (0%)
Frame = +1
Query: 292 ILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHN-QVSRIIETSHDDAC 468
+L+ TG+R IR +KE A I +++S + I+ N+E N + +I S + C
Sbjct: 50 VLDGFGGTGIRGIRISKETDSAVTI--SEVSPDSYRLIRDNVERNGSQASVINDSFE--C 105
Query: 469 MLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCY 648
+L +H + + +D+DPYG P +LD+A+ ++ G L VTATD + L G+ P
Sbjct: 106 VL----QHGAYEY--VDVDPYGSPVPYLDAALMGVKRNGFLGVTATDQSALTGSVPHKTR 159
Query: 649 CKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
+Y A+ HEM +R+++ + + A R+I P+
Sbjct: 160 IRYDALIKNDTFRHEMGIRLLIGYMAKRAASLGRFIDPL 198
>UniRef50_Q2FN43 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;
n=1; Methanospirillum hungatei JF-1|Rep: TRNA
(Guanine-N(2)-)-methyltransferase - Methanospirillum
hungatei (strain JF-1 / DSM 864)
Length = 370
Score = 77.0 bits (181), Expect(2) = 2e-13
Identities = 54/157 (34%), Positives = 82/157 (52%)
Frame = +1
Query: 295 LEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACML 474
L+A++A+G+R R E + ND + A++ I HN++ + I DA L
Sbjct: 57 LDAMAASGIRGCRVGYET--GVPVTFNDRDQLAIDLITHNVQSLGIKADITCR--DANSL 112
Query: 475 MYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCK 654
M K F +DLDP+G P+ F+D+A++S G L VTATD A L G + +
Sbjct: 113 MSDEK-----FGFVDLDPFGTPAPFIDAAIRS--SGKYLGVTATDTAPLCGAHLKAGIRR 165
Query: 655 YGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
Y A L T+ E+ LRI+L + +HA Y + I P+
Sbjct: 166 YMARPLNTEYHTEVGLRILLGNVARHAAVYDKGITPL 202
Score = 21.8 bits (44), Expect(2) = 2e-13
Identities = 9/21 (42%), Positives = 12/21 (57%)
Frame = +1
Query: 112 TEKVFYNPVQEFNRDLSIAVL 174
T VFYN NRD ++ V+
Sbjct: 28 TAPVFYNSRMALNRDSTVLVV 48
>UniRef50_Q6L2J7 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase; n=2; Thermoplasmatales|Rep:
N2,N2-dimethylguanosine tRNA methyltransferase -
Picrophilus torridus
Length = 332
Score = 72.1 bits (169), Expect(2) = 6e-13
Identities = 44/157 (28%), Positives = 80/157 (50%)
Frame = +1
Query: 295 LEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACML 474
L+A TG+R IR E T + +++++++ E I+ NI N +E ++ +
Sbjct: 53 LDAFGGTGVRGIRINVET--GTRTVISEINKKSFEYIEKNINENNAD--VEAYNEGFECV 108
Query: 475 MYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCK 654
+ K+ F ID+DPYG ++D A+ I++ G + +TATD+ L G++ + +
Sbjct: 109 LDKYL-----FDYIDIDPYGSIVPYIDKAISRIRNHGYIGITATDLTALTGSNVQKLKRR 163
Query: 655 YGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
Y A +L HE +RI++ +HA R P+
Sbjct: 164 YNAFALNDSFRHETGIRILIAFFVRHAAAMDRGAFPL 200
Score = 24.6 bits (51), Expect(2) = 6e-13
Identities = 8/17 (47%), Positives = 14/17 (82%)
Frame = +1
Query: 124 FYNPVQEFNRDLSIAVL 174
FYN Q+FNRD++++ +
Sbjct: 28 FYNADQKFNRDVTVSFI 44
>UniRef50_Q4BYN1 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;
n=2; Chroococcales|Rep: TRNA
(Guanine-N(2)-)-methyltransferase - Crocosphaera
watsonii
Length = 373
Score = 74.1 bits (174), Expect = 3e-12
Identities = 45/163 (27%), Positives = 82/163 (50%)
Frame = +1
Query: 277 EPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSH 456
+ K+ +L+ ++ G+RSIRY E A I +ND + + ETI++N+ + S+
Sbjct: 44 QEKLRVLDVMTGCGVRSIRYYLESD-ADYIWSNDSNPENKETIEYNLGFILQENKGKISY 102
Query: 457 DDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSP 636
+A + ++ + + +D+D +G P+ +L +A+ + + GGL+ +T TD G+ P
Sbjct: 103 QNANNIFFECYNNKDYYDLVDVDAFGSPNPYLSTALWATKIGGLIYLTCTDGRTGTGHLP 162
Query: 637 ETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
E C YG+ E LR+++ Q A I PI
Sbjct: 163 EKCLQVYGSYGRSHPAAQEQVLRLIIGSALQQAATMGLGIEPI 205
>UniRef50_Q9YDY7 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=1; Aeropyrum
pernix|Rep: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Aeropyrum pernix
Length = 407
Score = 73.7 bits (173), Expect = 4e-12
Identities = 64/220 (29%), Positives = 101/220 (45%), Gaps = 4/220 (1%)
Frame = +1
Query: 118 KVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITIL 297
+VFYNP EFNRD+S+ A L R T SG
Sbjct: 39 EVFYNPAMEFNRDVSVVA---------ASALRR---------TGLLTRSG-------VAF 73
Query: 298 EALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQV---SRIIETSHDDAC 468
+A + G+R +RYA E Y +I ND++ +A N N + S +I ++
Sbjct: 74 DAHAGVGVRGVRYAVEAGYV-KVIMNDINPKASMLAALNARANGLEPGSYMIFNKESNSL 132
Query: 469 MLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCY 648
M + P+ + ID+DPYG P+ F+D+A+ G ++ +TATD+AVL G
Sbjct: 133 MFHLSRERPTP-VSLIDIDPYGSPAPFVDAALALSGKGTVVAMTATDLAVLEGGKARAAV 191
Query: 649 CKYGAVSL-KTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
+Y S+ KT E LR++L + + A + + + P+
Sbjct: 192 RRYMLRSVSKTPVSKETGLRVLLGYVARVAAAHDKAVKPL 231
>UniRef50_P57705 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase); n=4;
Halobacteriaceae|Rep: N(2),N(2)-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2- dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase) - Halobacterium
salinarium (Halobacterium halobium)
Length = 371
Score = 72.1 bits (169), Expect = 1e-11
Identities = 64/217 (29%), Positives = 100/217 (46%)
Frame = +1
Query: 115 EKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITI 294
+ VF+N QE NRDL++A L A F +++ + +
Sbjct: 24 DDVFFNATQELNRDLTVATL------------AAFREREPRAASY--------------- 56
Query: 295 LEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACML 474
L+A++A+G+R +R A ++ D+ AVE N+ N + E DA L
Sbjct: 57 LDAMTASGIRGVRAANA---GWDVTMADVDADAVELATSNLARNGLDG--EVVARDANSL 111
Query: 475 MYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCK 654
++ H + +D+DP+G P F D+A + ++ L+ VTATD A L G E+ K
Sbjct: 112 LHDH---DRVLDVVDIDPFGSPMPFADAAFANARN--LVCVTATDTAPLCGAHFESGVRK 166
Query: 655 YGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
Y A T EM LRI+L + + A RY + PI
Sbjct: 167 YSATPRNTNYHAEMGLRILLGALARTAARYDVGVTPI 203
>UniRef50_Q0IBQ7 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase; n=13; Cyanobacteria|Rep:
N2,N2-dimethylguanosine tRNA methyltransferase -
Synechococcus sp. (strain CC9311)
Length = 416
Score = 71.7 bits (168), Expect = 2e-11
Identities = 46/173 (26%), Positives = 87/173 (50%), Gaps = 6/173 (3%)
Frame = +1
Query: 265 GGNPEPKITILEALSATGLRSIRYAKEIPYATN----IIANDLSEQAVETIKHNIEHNQ- 429
G + + + L+ ++ G+RS+R+ E A++ + ND ++ + N+E Q
Sbjct: 55 GASGDRPLRWLDLMAGCGIRSLRWGLEARRASHQQVELWVNDADQERGPLLAANLEPLQS 114
Query: 430 -VSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTAT 606
++ SH A L+ + + F IDLDP+GCP++ L S +Q+++ GG+LL+ +T
Sbjct: 115 CAGVVLIQSHQAAERLLREAYLEHRFFDLIDLDPFGCPNVLLQSTLQAMRFGGVLLLAST 174
Query: 607 DMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
D G+ ++GA + E+ALR+ L + + A R + P+
Sbjct: 175 DGRSPTGHDRFAAVRRFGAAARAHPSSWELALRLQLAALAREAWLLGRGLEPL 227
>UniRef50_Q4YVY3 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase, putative; n=3; Plasmodium
(Vinckeia)|Rep: N2,N2-dimethylguanosine tRNA
methyltransferase, putative - Plasmodium berghei
Length = 811
Score = 67.3 bits (157), Expect = 4e-10
Identities = 43/144 (29%), Positives = 78/144 (54%), Gaps = 4/144 (2%)
Frame = +1
Query: 343 EIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKR--FAAI 516
+I ++ A D E+ E K N+E ++IETS D+ K K S+R F I
Sbjct: 289 DISKNADLKARDEDEKEKEKDKTNVE----MKLIETSIDEKI----KKKQFSERYIFDII 340
Query: 517 DLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAV--SLKTKCCH 690
D+DPYG +L+S ++ + +L+T TDM +L G P+ + KY ++ + +
Sbjct: 341 DIDPYGSSICYLESCIKYGRSNFFMLITNTDMRILNGKFPDVSFYKYNSMIFNKNVNYNN 400
Query: 691 EMALRIMLQCIEQHANRYSRYIVP 762
E ++R++L ++ A++Y ++I+P
Sbjct: 401 EFSIRVLLYKLKNIASKYKKHIIP 424
Score = 59.3 bits (137), Expect = 1e-07
Identities = 44/123 (35%), Positives = 63/123 (51%), Gaps = 1/123 (0%)
Frame = +1
Query: 67 NLKTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLET 246
N K I EG +I + +FYN Q FNRD+SI VL +E Y K K +
Sbjct: 52 NNKHIYEGCVKIKNKNKHIFYNKAQVFNRDMSI-VLIKSLELYL---------KNKNKDN 101
Query: 247 VQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATN-IIANDLSEQAVETIKHNIEH 423
+ G N ++E LSA+G+RSIRY KE+ N I+ ND+ + A + I+ N +
Sbjct: 102 EKTIFRGFN------VIELLSASGIRSIRYVKELKETINHIVTNDIDKYACKQIRRNFKR 155
Query: 424 NQV 432
N +
Sbjct: 156 NNI 158
>UniRef50_A3DNY4 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase; n=1; Staphylothermus marinus F1|Rep:
N2,N2-dimethylguanosine tRNA methyltransferase -
Staphylothermus marinus (strain ATCC 43588 / DSM 3639 /
F1)
Length = 391
Score = 67.3 bits (157), Expect = 4e-10
Identities = 48/140 (34%), Positives = 68/140 (48%)
Frame = +1
Query: 295 LEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACML 474
+E L TG+R +R A E T II ND+ + I+ NI N+V + +A L
Sbjct: 70 VEPLGGTGVRGLRLALETN-GTGII-NDVDPISYYYIRRNIILNKVMDKVFPCLHEANAL 127
Query: 475 MYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCK 654
+ ID+DPYG P F+DSAV+ + LL +TATD L + +
Sbjct: 128 LNSLTFSGLSIDYIDIDPYGSPIPFIDSAVKPLGKKSLLGITATDTGPLNCSHKNKALRR 187
Query: 655 YGAVSLKTKCCHEMALRIML 714
YG +KT E+ LRI+L
Sbjct: 188 YGIRCIKTDFSRELGLRILL 207
>UniRef50_Q8NC68 Cluster: Uncharacterized protein C1orf25; n=46;
Euteleostomi|Rep: Uncharacterized protein C1orf25 - Homo
sapiens (Human)
Length = 733
Score = 65.7 bits (153), Expect = 1e-09
Identities = 45/171 (26%), Positives = 87/171 (50%), Gaps = 17/171 (9%)
Frame = +1
Query: 295 LEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDAC-- 468
L+A ATG+ +++AK + A + NDL+E +V I+ N N++ ++++ +
Sbjct: 280 LDAFGATGIMGLQWAKHLGNAVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEKSDD 339
Query: 469 MLMYKHKHPS---------------KRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTA 603
+L K+ + F I LDP+G +LDSA ++I++ G++ VT+
Sbjct: 340 ILEEGEKNLGNIKVTKMDANVLMHLRSFDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTS 399
Query: 604 TDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYI 756
TD++ L + YG ++T+ E+A RI++ + + A R ++ I
Sbjct: 400 TDISSLYAKAQHVARRHYGCNIVRTEYYKELAARIVVAAVARAAARCNKGI 450
>UniRef50_A5JZI1 Cluster: N(2),N(2)-dimethylguanosine tRNA
methyltransferase, putative; n=2; Plasmodium|Rep:
N(2),N(2)-dimethylguanosine tRNA methyltransferase,
putative - Plasmodium vivax
Length = 949
Score = 64.5 bits (150), Expect = 3e-09
Identities = 44/125 (35%), Positives = 67/125 (53%), Gaps = 1/125 (0%)
Frame = +1
Query: 67 NLKTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLET 246
N K I EG +I + +FYN Q FNRD+SI +L +E Y K
Sbjct: 67 NSKYIFEGSVKIKNKSNHIFYNKAQVFNRDMSI-ILIKALEIYLKNK------------- 112
Query: 247 VQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATN-IIANDLSEQAVETIKHNIEH 423
++++G ++E LSA+G+RSIRY KE+ N IIAND+ + A + I+ N +
Sbjct: 113 --NKDNGKILFRGFNVVELLSASGIRSIRYVKELRETINHIIANDIDKYACKQIRRNFKR 170
Query: 424 NQVSR 438
NQ+++
Sbjct: 171 NQINK 175
Score = 54.8 bits (126), Expect = 2e-06
Identities = 27/88 (30%), Positives = 50/88 (56%), Gaps = 2/88 (2%)
Frame = +1
Query: 505 FAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAV--SLKT 678
F ID+DPYG +L+S ++ + +L+T TDM VL G P+ + KY ++ S +
Sbjct: 400 FDIIDIDPYGSSIEYLESCLKYGRSNFFILITNTDMRVLNGKFPDVSFYKYNSMIFSKRV 459
Query: 679 KCCHEMALRIMLQCIEQHANRYSRYIVP 762
E ++R++ I+ A++Y + ++P
Sbjct: 460 HYNKEYSIRVLFYKIKTIASKYKKCVIP 487
>UniRef50_Q24FJ5 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase; n=1; Tetrahymena thermophila
SB210|Rep: N2,N2-dimethylguanosine tRNA
methyltransferase - Tetrahymena thermophila SB210
Length = 828
Score = 62.5 bits (145), Expect = 1e-08
Identities = 56/223 (25%), Positives = 102/223 (45%), Gaps = 7/223 (3%)
Frame = +1
Query: 118 KVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITIL 297
K+FYN VQ RD+++ V + +D LE + G IL
Sbjct: 307 KMFYNLVQLVERDITLLVANQYFKD---------------LEITNGQSFKG-----ARIL 346
Query: 298 EALSATGLRSIRYAKEIP--YATNIIANDLSEQAVETIKHNIEHNQV--SRIIETSHDDA 465
+ +++G++++RYAKEI Y +I + E K +N + S+ +T+H
Sbjct: 347 QGKASSGVKALRYAKEIDPSYIQDITVIEKDINFPEYFKLLAHYNDIDMSKFKKTNHFYL 406
Query: 466 CML---MYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSP 636
+ M K+ P + + ID++ YG + A++ +++GG++ TDM+ L G +
Sbjct: 407 SKILENMDKYDQP-ELYDFIDIEEYGSSIDTIRQAIKLVKNGGMISAMFTDMSTLCGPNV 465
Query: 637 ETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
CY Y ++ +E A+RI+ I A + + I P+
Sbjct: 466 IKCYSSYNKFRIRLASLNENAIRIIYSTILDIATQNNYSIEPV 508
>UniRef50_Q74ML2 Cluster: NEQ108; n=1; Nanoarchaeum equitans|Rep:
NEQ108 - Nanoarchaeum equitans
Length = 352
Score = 61.3 bits (142), Expect = 3e-08
Identities = 46/159 (28%), Positives = 78/159 (49%), Gaps = 1/159 (0%)
Frame = +1
Query: 292 ILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSR-IIETSHDDAC 468
+L+ +SA+G+R IRY E + ND++ A+E IK N+E N + I S+ DA
Sbjct: 49 VLDPMSASGIRGIRYYLE-SNIREVHFNDINPIAIEYIKKNLELNNIKEGKIIISNLDAR 107
Query: 469 MLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCY 648
+ K+++ ID+DP+G + +L + + L +T TD AV G Y
Sbjct: 108 AIEQKYEY-------IDIDPFGSATPYLYLINNTSK---YLAITYTDTAVWTGEKHRKAY 157
Query: 649 CKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
+Y +KT HE+ +R ++ + R ++PI
Sbjct: 158 IRYNFNVIKTPIKHEIGVRGLVAHLINKGAEIERALIPI 196
>UniRef50_Q31KF9 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;
n=2; Synechococcus elongatus|Rep: TRNA
(Guanine-N(2)-)-methyltransferase - Synechococcus sp.
(strain PCC 7942) (Anacystis nidulans R2)
Length = 381
Score = 60.9 bits (141), Expect = 3e-08
Identities = 39/150 (26%), Positives = 72/150 (48%)
Frame = +1
Query: 286 ITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDA 465
+ +LE ++ G+RS+RYA E ++ +D + ++ N+ RI + D A
Sbjct: 47 LNLLETMAGCGVRSLRYALEAK-VDRLVVSDADPELQPLLQQNLAPIASDRI-DLRCDSA 104
Query: 466 CMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETC 645
L + + + +D+D +G S L SA +++ GG L TATD L+G+ +
Sbjct: 105 RRLFAEAFAQQQFYDFVDVDAFGTASEHLASAWDAVKVGGCLYFTATDGRSLSGHDCDLA 164
Query: 646 YCKYGAVSLKTKCCHEMALRIMLQCIEQHA 735
+ YG + E LR+++ ++Q A
Sbjct: 165 FRAYGVWARSHPSIPEQGLRLLIAALQQQA 194
>UniRef50_Q8IEC3 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase, putative; n=2; Plasmodium|Rep:
N2,N2-dimethylguanosine tRNA methyltransferase, putative
- Plasmodium falciparum (isolate 3D7)
Length = 804
Score = 60.5 bits (140), Expect = 4e-08
Identities = 47/123 (38%), Positives = 63/123 (51%), Gaps = 1/123 (0%)
Frame = +1
Query: 73 KTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQ 252
K I EG +I +FYN Q FNRD+SI VL +E + EK K +K+
Sbjct: 51 KFIYEGSVKIKNKKNHIFYNKAQVFNRDMSI-VLIKALEIFMKEK----NKNNEKIIF-- 103
Query: 253 DEESGGNPEPKITILEALSATGLRSIRYAKEIPYATN-IIANDLSEQAVETIKHNIEHNQ 429
G N I+E LSA+G+RSIRYAKE+ N I ND+ + A + IK N N
Sbjct: 104 ---RGFN------IIELLSASGMRSIRYAKELEDTINHITTNDIDKYACKQIKRNFIRNN 154
Query: 430 VSR 438
+ +
Sbjct: 155 IKK 157
Score = 56.4 bits (130), Expect = 7e-07
Identities = 28/88 (31%), Positives = 51/88 (57%), Gaps = 2/88 (2%)
Frame = +1
Query: 505 FAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAV--SLKT 678
F ID+DPYG +L+S ++ + +L+T TDM +L G P+ + KY ++ S K
Sbjct: 309 FDIIDIDPYGSSIEYLESCLKYGRSNFFILITNTDMRILNGKFPDVSFYKYNSMIFSNKV 368
Query: 679 KCCHEMALRIMLQCIEQHANRYSRYIVP 762
+E ++R++ I+ A++Y + I+P
Sbjct: 369 NYNNEFSIRVLFYKIKIIASKYKKCIIP 396
>UniRef50_Q9LYL0 Cluster: N2, N2-dimethylguanosine tRNA
methyltransferases-like protein; n=5; core
eudicotyledons|Rep: N2, N2-dimethylguanosine tRNA
methyltransferases-like protein - Arabidopsis thaliana
(Mouse-ear cress)
Length = 433
Score = 59.7 bits (138), Expect = 8e-08
Identities = 44/164 (26%), Positives = 76/164 (46%), Gaps = 4/164 (2%)
Frame = +1
Query: 286 ITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIE----TS 453
+ +L+A+ G+RS+RY E A ++AND ++ I N+ + E +
Sbjct: 90 LRVLDAMCGCGIRSLRYLVEAE-ADFVMANDANDDNRRVITDNLSKVERGTGDERRWVVT 148
Query: 454 HDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNS 633
H A M + + F ID+D +G S FL A +++ GGLL +T+TD G+
Sbjct: 149 HMLANKAMIERYMVADFFDMIDIDSFGSDSSFLRDAFNALRLGGLLYLTSTDGYSSGGHR 208
Query: 634 PETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
P YGA +E+ LR+++ + A ++ P+
Sbjct: 209 PYNSLAAYGAFIRPMPFGNEIGLRMLIGGAVREAALLGYHVTPL 252
>UniRef50_UPI0000E49722 Cluster: PREDICTED: hypothetical protein,
partial; n=1; Strongylocentrotus purpuratus|Rep:
PREDICTED: hypothetical protein, partial -
Strongylocentrotus purpuratus
Length = 468
Score = 59.3 bits (137), Expect = 1e-07
Identities = 30/82 (36%), Positives = 46/82 (56%)
Frame = +1
Query: 505 FAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKC 684
F I LDPYGC + F+DSA +I + G+L +T+TD A L G P+ YG K +
Sbjct: 147 FDFIFLDPYGCATPFMDSAFSNISNNGILAITSTDTASLYGKCPQVTLRNYGGQVAKCEY 206
Query: 685 CHEMALRIMLQCIEQHANRYSR 750
E+A R+++ + A R ++
Sbjct: 207 LKELAGRLVVAAAVRAAARCNK 228
>UniRef50_Q00YF6 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase f; n=2; Ostreococcus|Rep:
N2,N2-dimethylguanosine tRNA methyltransferase f -
Ostreococcus tauri
Length = 471
Score = 53.6 bits (123), Expect = 5e-06
Identities = 39/164 (23%), Positives = 75/164 (45%), Gaps = 7/164 (4%)
Frame = +1
Query: 295 LEALSATGLRSIRYAKEIPYATN---IIANDLSEQAVETIKHNIEHNQVSRIIETS--HD 459
L+A T R+A+ N + D+S + + N++ + SR +ETS D
Sbjct: 88 LDACDGTCASGARFARMAACGVNLRSVTCVDVSADVRDALNANLDAHVRSRGVETSCAFD 147
Query: 460 DACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPE 639
D+ + + + + +D+D +G + F DSA++ ++ GG T+TD L G + E
Sbjct: 148 DSQRVFARKWLAGELYDYVDVDGFGSAN-FADSALRIVRHGGYFYATSTDGRALCGQNAE 206
Query: 640 TCYCKYG--AVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
C +G VS +E A+R+ + + + + + P+
Sbjct: 207 RCATAFGNSIVSPSRPSVNETAVRVFIGDVVRRGSALKLRVTPV 250
>UniRef50_UPI000155D1C2 Cluster: PREDICTED: similar to TRM1 tRNA
methyltransferase 1 homolog (S. cerevisiae); n=1;
Ornithorhynchus anatinus|Rep: PREDICTED: similar to TRM1
tRNA methyltransferase 1 homolog (S. cerevisiae) -
Ornithorhynchus anatinus
Length = 223
Score = 48.8 bits (111), Expect = 1e-04
Identities = 27/58 (46%), Positives = 33/58 (56%), Gaps = 1/58 (1%)
Frame = +1
Query: 79 IKEGQAEICLT-TEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETV 249
+ EG A I +VFYNPVQEFNRDL+ AV+T F A K R K ++TV
Sbjct: 59 VTEGAARIVFPGANEVFYNPVQEFNRDLTCAVVTEFARLQLAAKGIRIHHKNSPVQTV 116
>UniRef50_Q8CX51 Cluster: tRNA (uracil-5-)-methyltransferase (EC
2.1.1.35) (tRNA(M-5-U54)- methyltransferase); n=20;
Gammaproteobacteria|Rep: tRNA
(uracil-5-)-methyltransferase (EC 2.1.1.35)
(tRNA(M-5-U54)- methyltransferase) - Shewanella
oneidensis
Length = 366
Score = 46.4 bits (105), Expect = 8e-04
Identities = 35/119 (29%), Positives = 57/119 (47%), Gaps = 2/119 (1%)
Frame = +1
Query: 292 ILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVS--RIIETSHDDA 465
+LE G SI A+ ++A +L++ +V+ ++NIE N + +II S ++
Sbjct: 213 LLELYCGNGNFSIALAQNF---NRVLATELAKPSVDAAQYNIEINGIDNLQIIRMSAEEF 269
Query: 466 CMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPET 642
M K K +R IDLD Y C +IF+D I L LV + + +P+T
Sbjct: 270 SDAMAK-KRSFRRLEGIDLDSYLCNTIFVDPPRAGIDPATLDLVQGYERILYISCNPDT 327
>UniRef50_Q6LLU2 Cluster: tRNA (uracil-5-)-methyltransferase (EC
2.1.1.35) (tRNA(M-5-U54)- methyltransferase); n=42;
Proteobacteria|Rep: tRNA (uracil-5-)-methyltransferase
(EC 2.1.1.35) (tRNA(M-5-U54)- methyltransferase) -
Photobacterium profundum (Photobacterium sp. (strain
SS9))
Length = 368
Score = 42.3 bits (95), Expect = 0.013
Identities = 39/147 (26%), Positives = 68/147 (46%), Gaps = 6/147 (4%)
Frame = +1
Query: 220 EKKQKKLETVQD--EESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQA 393
E QK LE D ++S G+ +LE G S+ AK ++A +L++ +
Sbjct: 194 EVAQKMLEWAVDCTQDSDGD------LLELYCGNGNFSLALAKNFE---RVLATELAKPS 244
Query: 394 VETIKHNIEHNQVS--RIIETSHDDACMLMYKHKHPSKRFAA--IDLDPYGCPSIFLDSA 561
VE+ ++NI N + +I+ S +D M K +R A +DL Y C +IF+D
Sbjct: 245 VESAQYNIAVNNIDNVQIVRMSAEDFTDAM-AGKREFRRLKAQNVDLQSYNCNTIFVDPP 303
Query: 562 VQSIQDGGLLLVTATDMAVLAGNSPET 642
+ +G +V + + +P+T
Sbjct: 304 RSGMDEGTCRMVQGYERIMYISCNPDT 330
>UniRef50_Q31JA4 Cluster: tRNA (uracil-5-)-methyltransferase (EC
2.1.1.35) (tRNA(M-5-U54)- methyltransferase); n=1;
Thiomicrospira crunogena XCL-2|Rep: tRNA
(uracil-5-)-methyltransferase (EC 2.1.1.35)
(tRNA(M-5-U54)- methyltransferase) - Thiomicrospira
crunogena (strain XCL-2)
Length = 374
Score = 40.3 bits (90), Expect = 0.051
Identities = 32/141 (22%), Positives = 57/141 (40%)
Frame = +1
Query: 220 EKKQKKLETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVE 399
E Q L + + NP+ + ++E G SI + + ++A ++S+ +V
Sbjct: 197 EMAQNMLHWARKVAAKANPDQQNDLIELYCGNGHFSIALSDQFH---RVLATEISKTSVA 253
Query: 400 TIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQD 579
+ + NIE N+V + + R IDL Y +IF+D + D
Sbjct: 254 SAQFNIEANKVDNVTVVKMAAEEISAALQGETFFRLKDIDLSAYAFNTIFVDPPRSGLDD 313
Query: 580 GGLLLVTATDMAVLAGNSPET 642
+VT D + +PET
Sbjct: 314 LTRQMVTEFDYIIYISCNPET 334
>UniRef50_A5URP6 Cluster: PUA domain containing protein; n=5;
Chloroflexi (class)|Rep: PUA domain containing protein -
Roseiflexus sp. RS-1
Length = 396
Score = 39.9 bits (89), Expect = 0.068
Identities = 19/58 (32%), Positives = 33/58 (56%)
Frame = +1
Query: 355 ATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDP 528
A +I A D SE A+ ++ + N V+ +ET+ DA L+ +++ +RF + LDP
Sbjct: 244 ARHITAVDTSEAALSMLREGLTLNMVATPVETAPGDAFKLLRRYREEQRRFDVVILDP 301
>UniRef50_Q8R9R9 Cluster: Predicted SAM-dependent
methyltransferases; n=12; Firmicutes|Rep: Predicted
SAM-dependent methyltransferases - Thermoanaerobacter
tengcongensis
Length = 391
Score = 39.5 bits (88), Expect = 0.089
Identities = 30/134 (22%), Positives = 61/134 (45%)
Frame = +1
Query: 127 YNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITILEAL 306
++PVQ+F + + +++ +K F +++ +Q+ G +L+
Sbjct: 175 FDPVQQFKEN----GIKFWVDMENGQKTGYFLDQKENRRAIQNYVKGAE------VLDCF 224
Query: 307 SATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMYKH 486
S TG ++ I+ D+SE+A+E K N+E N + E ++A L+ ++
Sbjct: 225 SHTGSFAVHALHYGAKRVEIV--DISEEALEMAKKNVELNGYQQRAEFIRENAFDLLRRY 282
Query: 487 KHPSKRFAAIDLDP 528
K+F + LDP
Sbjct: 283 DREKKKFDTVILDP 296
>UniRef50_Q197B1 Cluster: Putative uncharacterized protein; n=1;
Aedes taeniorhynchus iridescent virus|Rep: Putative
uncharacterized protein - Aedes taeniorhynchus
iridescent virus
Length = 683
Score = 38.7 bits (86), Expect = 0.16
Identities = 30/95 (31%), Positives = 49/95 (51%), Gaps = 11/95 (11%)
Frame = +1
Query: 187 EDYKAE---KLARFEKKQKKLETVQDEESGGNPEPKITILEALSATGLRSIRYAKEI--- 348
E++KAE KL FE+K T +DEE GGNPE T+ + ++ + + E+
Sbjct: 30 ENFKAEIHSKLDSFERKLNASPTYRDEEGGGNPEHYETLSQEINDLQSQIENLSLEVENL 89
Query: 349 ---PYATNIIANDLSE--QAVETIKHNIEHNQVSR 438
+ + +A L+E Q++ TIK +E N+ R
Sbjct: 90 QGSSSSPSNVAAALAELSQSIRTIKEQLEANRKER 124
>UniRef50_Q8GDQ7 Cluster: Methyltransferase; n=1; Heliobacillus
mobilis|Rep: Methyltransferase - Heliobacillus mobilis
Length = 319
Score = 38.3 bits (85), Expect = 0.21
Identities = 24/75 (32%), Positives = 38/75 (50%)
Frame = +1
Query: 202 EKLARFEKKQKKLETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDL 381
EK+A F K+Q K G +I + + + +G ++ AKE+ N+IA DL
Sbjct: 118 EKMAGFPKRQDKGCDDSPPAQGSVAGKEIWLADVGTGSGAIALAMAKELR-CVNVIATDL 176
Query: 382 SEQAVETIKHNIEHN 426
S +A+ T + N E N
Sbjct: 177 SPEALATARGNAERN 191
>UniRef50_Q011B2 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase family protein; n=1; Ostreococcus
tauri|Rep: N2,N2-dimethylguanosine tRNA
methyltransferase family protein - Ostreococcus tauri
Length = 298
Score = 38.3 bits (85), Expect = 0.21
Identities = 15/32 (46%), Positives = 23/32 (71%)
Frame = +1
Query: 670 LKTKCCHEMALRIMLQCIEQHANRYSRYIVPI 765
L+ K CHE A+RI+L +E A ++ R+IVP+
Sbjct: 9 LRAKYCHEAAVRILLAAVENAAIKHKRHIVPV 40
>UniRef50_P39541 Cluster: Uncharacterized protein YJL195C; n=1;
Saccharomyces cerevisiae|Rep: Uncharacterized protein
YJL195C - Saccharomyces cerevisiae (Baker's yeast)
Length = 233
Score = 37.1 bits (82), Expect = 0.48
Identities = 18/37 (48%), Positives = 25/37 (67%)
Frame = +1
Query: 568 SIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKT 678
S D L+LVTATD ++ +GNS + +CK+G V L T
Sbjct: 26 SPNDIQLILVTATDSSLPSGNSNQDKFCKFGFVCLST 62
>UniRef50_UPI00006CA4AE Cluster: hypothetical protein
TTHERM_00498120; n=1; Tetrahymena thermophila SB210|Rep:
hypothetical protein TTHERM_00498120 - Tetrahymena
thermophila SB210
Length = 382
Score = 36.7 bits (81), Expect = 0.63
Identities = 31/146 (21%), Positives = 64/146 (43%)
Frame = +1
Query: 7 NEQNKCFYINNIFMRMETISNLKTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFI 186
N +N+ + ++ I+M +ET + + K + + + FYN + + L+L +
Sbjct: 193 NYENEIYEMSLIYMNLETKTPVLKQKVFSKNLIMFITRDFYNFYSKLSCQNMKENLSLIL 252
Query: 187 EDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNI 366
+ + + E + K ++ + E+ E +I E L + I + Y T
Sbjct: 253 HGDEFDGRLQLENRIKMMKQTLNGETCIQVEDEIITAEGLVIPTTKQI-HLYFYDYQTRE 311
Query: 367 IANDLSEQAVETIKHNIEHNQVSRII 444
I+ + + IK+N E NQ+ R+I
Sbjct: 312 ISKKHNYFFIHLIKYNFEANQIQRLI 337
>UniRef50_Q756L4 Cluster: AER240Wp; n=1; Eremothecium gossypii|Rep:
AER240Wp - Ashbya gossypii (Yeast) (Eremothecium
gossypii)
Length = 390
Score = 36.3 bits (80), Expect = 0.83
Identities = 26/88 (29%), Positives = 43/88 (48%), Gaps = 1/88 (1%)
Frame = +1
Query: 283 KITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDD 462
K +LE + TGL I YA A ++ DL E V ++HN+ N ++ + + D
Sbjct: 229 KKRVLELGAGTGLVGIAYALANIDADDVFVTDLPE-IVPNLRHNLALNNLTNVRASVLDW 287
Query: 463 ACMLMYKHKHPSKRFAAIDL-DPYGCPS 543
+ + H+H +F AI + DP P+
Sbjct: 288 SDPTSFLHEHGELQFDAIFVADPIYSPN 315
>UniRef50_UPI0001509CE7 Cluster: cyclic nucleotide-binding domain
containing protein; n=1; Tetrahymena thermophila
SB210|Rep: cyclic nucleotide-binding domain containing
protein - Tetrahymena thermophila SB210
Length = 742
Score = 35.9 bits (79), Expect = 1.1
Identities = 27/95 (28%), Positives = 49/95 (51%), Gaps = 2/95 (2%)
Frame = +1
Query: 1 EFNEQNKCFYINNIFMRME-TISNLKTIKEGQAEICLTTEKVFYNPVQ-EFNRDLSIAVL 174
+ N++ K Y NI ++ I N + KE ++CL+ E+VFY+P Q FN S
Sbjct: 156 DLNDEIKLEYYKNILQKVPFLIGNFQ--KETIDKLCLSIEEVFYSPNQIIFNEQESNDFS 213
Query: 175 TLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPE 279
+ I + + A+ + Q+ E +++E+ NP+
Sbjct: 214 LIVIVSGQGVR-AQLNQGQQIKEEIEEEQQFTNPQ 247
>UniRef50_Q1VMM5 Cluster: Ribosomal protein L11 methyltransferase;
n=1; Psychroflexus torquis ATCC 700755|Rep: Ribosomal
protein L11 methyltransferase - Psychroflexus torquis
ATCC 700755
Length = 245
Score = 35.9 bits (79), Expect = 1.1
Identities = 45/203 (22%), Positives = 92/203 (45%), Gaps = 5/203 (2%)
Frame = +1
Query: 19 KCFYINNIFMRMETISNLKT--IKEGQAEI--CLTTEKVFYNPVQEFNRDLSIAVLTLFI 186
+CF + + + + + LKT +K +I + T F N V++ NR +L I
Sbjct: 22 ECFAVKSAITKTKKKNWLKTSIVKFSPVKINKFIVTSSFFANSVRQ-NR------FSLII 74
Query: 187 EDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNI 366
+ A + LE +Q + N ++++++ TG+ +I AK +++ I
Sbjct: 75 DASLAFGTGHHYSTKFCLELIQQLKKSQNN--RLSVIDVGCGTGILAIAIAKL--FSSRI 130
Query: 367 IANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLD-PYGCPS 543
IA D AVE + NI N+VS+ ++ L+ H + ++ + + Y
Sbjct: 131 IAVDNDIHAVEMTRRNIVINKVSQYVKVYKSTG--LIGNHLNSGAKYDLVVANILYNPIK 188
Query: 544 IFLDSAVQSIQDGGLLLVTATDM 612
+ S +++ DGG L+++ ++
Sbjct: 189 SMMRSIAENLSDGGSLILSGLNV 211
>UniRef50_A4ST99 Cluster: Putative uncharacterized protein; n=2;
Gammaproteobacteria|Rep: Putative uncharacterized
protein - Aeromonas salmonicida (strain A449)
Length = 695
Score = 35.9 bits (79), Expect = 1.1
Identities = 31/101 (30%), Positives = 47/101 (46%)
Frame = +1
Query: 301 ALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMY 480
ALS S RYA +P +++ + E + + H + Q RI S DAC L
Sbjct: 530 ALSLASQDSFRYA--VPLEGDLV--EYCEARLSS--HQLGTEQAERI-HFSQGDACNLKP 582
Query: 481 KHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTA 603
K+ H AA +D P+ FL ++ GGLL++T+
Sbjct: 583 KYDHYDLVLAANLIDRLREPARFLRDIAPRLRSGGLLVLTS 623
>UniRef50_Q7QPA8 Cluster: GLP_122_5076_6419; n=1; Giardia lamblia
ATCC 50803|Rep: GLP_122_5076_6419 - Giardia lamblia ATCC
50803
Length = 447
Score = 35.9 bits (79), Expect = 1.1
Identities = 18/66 (27%), Positives = 33/66 (50%)
Frame = +1
Query: 280 PKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHD 459
P +L+ G ++ AK + TN+I NDL+ A +++K N+ N+ I ++
Sbjct: 254 PNDLVLDGTCGIGPHALLLAKRFNF-TNLICNDLNPDAYKSLKMNVRINKAENAITCFNE 312
Query: 460 DACMLM 477
D L+
Sbjct: 313 DVSSLL 318
>UniRef50_A1SHQ9 Cluster: Regulatory protein, TetR; n=1;
Nocardioides sp. JS614|Rep: Regulatory protein, TetR -
Nocardioides sp. (strain BAA-499 / JS614)
Length = 236
Score = 35.5 bits (78), Expect = 1.5
Identities = 21/59 (35%), Positives = 30/59 (50%)
Frame = +1
Query: 481 KHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKY 657
+H++ S R D++P G FL +AV + G T D+A LAG+SP Y Y
Sbjct: 21 EHEYVSPRGLFDDVEPKGSRR-FLTAAVAAFNGRGYHATTTRDIATLAGSSPAGIYTYY 78
>UniRef50_Q5VNJ0 Cluster: Putative tRNA-(N1G37) methyltransferase;
n=6; Oryza sativa|Rep: Putative tRNA-(N1G37)
methyltransferase - Oryza sativa subsp. japonica (Rice)
Length = 586
Score = 34.7 bits (76), Expect = 2.5
Identities = 20/58 (34%), Positives = 33/58 (56%)
Frame = +1
Query: 292 ILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDA 465
+ + S G +I A+++ Y + ANDL+ AVE ++ NI N++ R IE + DA
Sbjct: 414 VCDVFSGVGPIAISAARKVKY---VYANDLNPTAVEYLERNIVLNKLERKIEVFNMDA 468
>UniRef50_Q5PK68 Cluster: tRNA (uracil-5-)-methyltransferase (EC
2.1.1.35) (tRNA(M-5-U54)- methyltransferase); n=26;
cellular organisms|Rep: tRNA
(uracil-5-)-methyltransferase (EC 2.1.1.35)
(tRNA(M-5-U54)- methyltransferase) - Salmonella
paratyphi-a
Length = 366
Score = 34.7 bits (76), Expect = 2.5
Identities = 33/144 (22%), Positives = 60/144 (41%), Gaps = 2/144 (1%)
Frame = +1
Query: 277 EPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVS--RIIET 450
+ K +LE G S+ A+ ++A ++++ +V ++NI N + +II
Sbjct: 209 DSKGDLLELYCGNGNFSLALARNF---NRVLATEIAKPSVAAAQYNIAANHIDNVQIIRM 265
Query: 451 SHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGN 630
+ ++ M + R IDL Y C +IF+D + +V A +
Sbjct: 266 AAEEFTQAMNGVRE-FNRLQGIDLKRYQCETIFVDPPRSGLDSETEKMVQAYPRILYISC 324
Query: 631 SPETCYCKYGAVSLKTKCCHEMAL 702
+PET CK +T +AL
Sbjct: 325 NPET-LCKNLETLSQTHTVSRLAL 347
>UniRef50_Q749W6 Cluster: Putative uncharacterized protein; n=4;
Desulfuromonadales|Rep: Putative uncharacterized protein
- Geobacter sulfurreducens
Length = 396
Score = 34.3 bits (75), Expect = 3.4
Identities = 26/102 (25%), Positives = 39/102 (38%), Gaps = 1/102 (0%)
Frame = +1
Query: 355 ATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYG 534
A + D+SE+A + N N + + DA + +H +RF + LDP
Sbjct: 244 AAETLCLDVSERAAALVMANARLNGLGDRVRAEVCDAFERLASLRHEGRRFGVVVLDP-- 301
Query: 535 CPSIFLDSAVQSIQDGGLLLVTATDMAVL-AGNSPETCYCKY 657
P+ + G L V M +L G TC C Y
Sbjct: 302 -PAFVKSKKALKEAEKGYLTVNRRGMELLEEGGYLITCSCSY 342
>UniRef50_A6QBC0 Cluster: tRNA (Uracil-5-)-methyltransferase; n=1;
Sulfurovum sp. NBC37-1|Rep: tRNA
(Uracil-5-)-methyltransferase - Sulfurovum sp. (strain
NBC37-1)
Length = 371
Score = 34.3 bits (75), Expect = 3.4
Identities = 22/99 (22%), Positives = 45/99 (45%), Gaps = 2/99 (2%)
Frame = +1
Query: 352 YATNIIANDLSEQAVETIKHNIEHNQVSRII--ETSHDDACMLMYKHKHPSKRFAAIDLD 525
Y N++A ++S++++ N E N V I + ++ + + S R IDL
Sbjct: 236 YFDNVLATEISKRSIHAALQNCELNAVENITFARLASEEMTEALNGVREFS-RLKGIDLK 294
Query: 526 PYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPET 642
Y ++ +D + +G + L++ D + +PET
Sbjct: 295 SYDFSTVLVDPPRAGLDEGTIELISNIDNIIYISCNPET 333
>UniRef50_A6EDS8 Cluster: PUA domain containing protein; n=1;
Pedobacter sp. BAL39|Rep: PUA domain containing protein
- Pedobacter sp. BAL39
Length = 394
Score = 34.3 bits (75), Expect = 3.4
Identities = 29/111 (26%), Positives = 48/111 (43%), Gaps = 3/111 (2%)
Frame = +1
Query: 355 ATNIIANDLSEQAVETIKHNIEHNQVSRIIETS-HDDACMLMYKHKHPSKRFAAIDLDP- 528
A + + D S A+ET++HN++ N ++ S D + K ++F I LDP
Sbjct: 241 AAEVTSVDSSPLAIETLQHNLQLNGFDASLQQSIQSDVNKQLRVFKEEGRKFDVIVLDPP 300
Query: 529 -YGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKT 678
Y LD A ++ +D + M +L Y GAV ++T
Sbjct: 301 KYAPSRSALDRAARAYKD-----LNRLGMLLLEPGGIMATYSCSGAVDMET 346
>UniRef50_Q0W088 Cluster: Putative SAM-dependent methyltransferase;
n=1; uncultured methanogenic archaeon RC-I|Rep: Putative
SAM-dependent methyltransferase - Uncultured
methanogenic archaeon RC-I
Length = 337
Score = 34.3 bits (75), Expect = 3.4
Identities = 28/100 (28%), Positives = 50/100 (50%)
Frame = +1
Query: 292 ILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACM 471
+++ + G +I AK+ Y ++A D + AVE +K NI+ N V+ I + C
Sbjct: 184 VVDMFAGIGPFAIPAAKKAMY---VVAVDKNPYAVEYMKRNIQINHVTNI-----EAVCA 235
Query: 472 LMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLL 591
+ + K P + AI P+ FLD A + ++ GG++
Sbjct: 236 DVREIKLPQQADRAIMNLPHSAHE-FLDKAFELVRTGGII 274
>UniRef50_Q7ULT2 Cluster: HemK protein; n=1; Pirellula sp.|Rep: HemK
protein - Rhodopirellula baltica
Length = 296
Score = 33.9 bits (74), Expect = 4.4
Identities = 31/130 (23%), Positives = 59/130 (45%), Gaps = 3/130 (2%)
Frame = +1
Query: 214 RFEKKQKKLETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQA 393
R E + +E + + + P T+L+ + +G ++ AK +P T + A D+S A
Sbjct: 103 RPETEHLVIEAIDQIKGRLSDRPSPTVLDIGTGSGAIAVAIAKSLP-KTQVTAVDISLTA 161
Query: 394 VETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAI-DLDPYGCPSIF--LDSAV 564
++ K N+E+ ++S + D ++ P + F I PY S + L + V
Sbjct: 162 LDIAKWNVENLKLSDRVTLLQSD----LFDGLEPDQTFDVICSNPPYISQSEYDELPTTV 217
Query: 565 QSIQDGGLLL 594
+ + G LL
Sbjct: 218 REFEPRGALL 227
>UniRef50_Q4E860 Cluster: Uncharacterized ACR, YhhQ family COG1738
family; n=3; Wolbachia|Rep: Uncharacterized ACR, YhhQ
family COG1738 family - Wolbachia endosymbiont of
Drosophila simulans
Length = 166
Score = 33.9 bits (74), Expect = 4.4
Identities = 18/44 (40%), Positives = 25/44 (56%)
Frame = -2
Query: 134 GL*KTFSVVKHISAWPSFIVFKFDIVSILIKMLFI*KHLFCSLN 3
GL FS K +S + ++FKF VS+L LF+ +LFC N
Sbjct: 119 GLLYKFSAGKCLSIYIRDLIFKFSYVSVLSICLFVGMYLFCLAN 162
>UniRef50_A6EWY0 Cluster: Putative uncharacterized protein; n=1;
Marinobacter algicola DG893|Rep: Putative
uncharacterized protein - Marinobacter algicola DG893
Length = 398
Score = 33.9 bits (74), Expect = 4.4
Identities = 21/84 (25%), Positives = 37/84 (44%)
Frame = +1
Query: 412 NIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLL 591
N H Q+S+ +E + DDAC L +H ++ + P+ C S+ + D +L
Sbjct: 165 NFSHTQISKPVEFALDDACFLEKRHNDYTRGIGTKNA-PFACVSM---RKFGGMDDDDVL 220
Query: 592 LVTATDMAVLAGNSPETCYCKYGA 663
V L+ N+ T + + A
Sbjct: 221 RVATVIEKALSSNNVNTVFIPHHA 244
>UniRef50_A4J7F1 Cluster: Ribosomal protein L11 methyltransferase;
n=1; Desulfotomaculum reducens MI-1|Rep: Ribosomal
protein L11 methyltransferase - Desulfotomaculum
reducens MI-1
Length = 308
Score = 33.9 bits (74), Expect = 4.4
Identities = 19/56 (33%), Positives = 31/56 (55%)
Frame = +1
Query: 289 TILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSH 456
++ + + TG+ +I AK A ++A DL E AV+ + N+E N V I+E H
Sbjct: 173 SVADVGTGTGILAITSAKL--GAARVLAVDLDEVAVKVSQENVERNGVQDIVEVFH 226
>UniRef50_A0C933 Cluster: Chromosome undetermined scaffold_16, whole
genome shotgun sequence; n=3; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_16,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 783
Score = 33.9 bits (74), Expect = 4.4
Identities = 20/71 (28%), Positives = 38/71 (53%)
Frame = +1
Query: 250 QDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQ 429
QDEE+G K+ + S + I++ +EI Y + D SE + ++K N ++++
Sbjct: 443 QDEENGVFIRLKLDQQKVSSLSVESKIKFKEEIIYKWKLDKIDFSEGDLHSLKINYQNDE 502
Query: 430 VSRIIETSHDD 462
+S II+ + D
Sbjct: 503 LSLIIDDTQSD 513
>UniRef50_UPI00006CAA99 Cluster: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain containing protein; n=1;
Tetrahymena thermophila SB210|Rep: ATPase, histidine
kinase-, DNA gyrase B-, and HSP90-like domain containing
protein - Tetrahymena thermophila SB210
Length = 1662
Score = 33.5 bits (73), Expect = 5.9
Identities = 36/149 (24%), Positives = 64/149 (42%), Gaps = 1/149 (0%)
Frame = +1
Query: 1 EFNEQNKCFY-INNIFMRMETISNLKTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLT 177
EF+ +K F +NN F + + N K K+ Q + E + EFN ++
Sbjct: 1257 EFSSSDKDFKNLNNCFSKSSSKENSKVQKQDQKDNVNLYENFLQKKLSEFN---GVSAQN 1313
Query: 178 LFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYA 357
Y + EK QK T Q++ S + + L+ ++S+ + I Y
Sbjct: 1314 TNRSSYYQN---QNEKTQK--NTQQNDNS-------MIVNNQLNGNNIKSLEHMNNIFYN 1361
Query: 358 TNIIANDLSEQAVETIKHNIEHNQVSRII 444
++ SE + + I HNI++NQ++ I
Sbjct: 1362 QMQLSEQFSETSWQ-IPHNIQNNQINSTI 1389
>UniRef50_A6LYS3 Cluster: Sensor protein; n=1; Clostridium
beijerinckii NCIMB 8052|Rep: Sensor protein -
Clostridium beijerinckii NCIMB 8052
Length = 1036
Score = 33.5 bits (73), Expect = 5.9
Identities = 29/112 (25%), Positives = 49/112 (43%), Gaps = 6/112 (5%)
Frame = +1
Query: 31 INNIFMRMETISNLKTIKEGQAEICLTTEKVFYN--PVQEFNRDLSIAVLTLFIEDYKAE 204
I+ +F I+++ T+K TT+ + N P+ + N ++ AVL + E
Sbjct: 438 ISKVFRGEVVINDIMTLKNAT-----TTKHISLNGRPIYDSNGNIKFAVLCCHDITHDIE 492
Query: 205 KLARFEKKQKKLETVQDEESGG----NPEPKITILEALSATGLRSIRYAKEI 348
E++ KKLE + S G NP+ +T+L S + I K I
Sbjct: 493 AQMLIEQQNKKLEAIISSASDGMFLFNPDNSVTLLNKESEKFIYDINGYKSI 544
>UniRef50_A6C404 Cluster: Putative uncharacterized protein; n=1;
Planctomyces maris DSM 8797|Rep: Putative
uncharacterized protein - Planctomyces maris DSM 8797
Length = 252
Score = 33.5 bits (73), Expect = 5.9
Identities = 22/78 (28%), Positives = 39/78 (50%)
Frame = +1
Query: 250 QDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQ 429
Q + S G+ +P + IL+ + T L I + + IIA DL+ + ++ + NI+
Sbjct: 67 QQQASAGDSQP-LQILDLGTGTALIPIEICQRVSQL-QIIATDLAAEMLKVAQQNIQRAG 124
Query: 430 VSRIIETSHDDACMLMYK 483
+ + I H DA +L K
Sbjct: 125 LDKSILLEHADAKLLPCK 142
>UniRef50_Q01C22 Cluster: TRNA methyltransferase; n=1; Ostreococcus
tauri|Rep: TRNA methyltransferase - Ostreococcus tauri
Length = 123
Score = 33.5 bits (73), Expect = 5.9
Identities = 21/55 (38%), Positives = 32/55 (58%), Gaps = 2/55 (3%)
Frame = +1
Query: 79 IKEGQAEIC--LTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKK 237
I+EG A T+ VFYN Q NRD+S+AV+ F + +AE+ A +++K
Sbjct: 19 IREGAARALQRAETDDVFYNKPQVVNRDMSLAVIREF-QRVRAEEHASGTSRREK 72
>UniRef50_Q61PE0 Cluster: Putative uncharacterized protein CBG07583;
n=1; Caenorhabditis briggsae|Rep: Putative
uncharacterized protein CBG07583 - Caenorhabditis
briggsae
Length = 702
Score = 33.5 bits (73), Expect = 5.9
Identities = 21/84 (25%), Positives = 38/84 (45%)
Frame = +1
Query: 61 ISNLKTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKL 240
+S K ++ +A +E+ NP+ E + + IA + L +E EK+ +K +
Sbjct: 395 VSTSKQNEKKEAVKSTLSEREISNPLFEGEQSVKIASVPLKVETKPVEKIVEIQKPVANV 454
Query: 241 ETVQDEESGGNPEPKITILEALSA 312
ET + E P + +EA A
Sbjct: 455 ETKKSETVETKTAPPVAKVEAKQA 478
>UniRef50_A7RS85 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 210
Score = 33.5 bits (73), Expect = 5.9
Identities = 22/55 (40%), Positives = 31/55 (56%), Gaps = 3/55 (5%)
Frame = +1
Query: 184 IEDYKAEKLARFEK--KQKKLETVQDEESGGNPEPKIT-ILEALSATGLRSIRYA 339
I +++A+ A EK KQK+ ET+ DE NP K+ +LEA A RS+ A
Sbjct: 84 ITEHQAKLAAEQEKLQKQKQQETIHDEPLEENPNQKMAELLEAEGAVEARSVEEA 138
>UniRef50_Q8TYV2 Cluster: N2,N2-dimethylguanosine tRNA
methyltransferase; n=1; Methanopyrus kandleri|Rep:
N2,N2-dimethylguanosine tRNA methyltransferase -
Methanopyrus kandleri
Length = 293
Score = 33.5 bits (73), Expect = 5.9
Identities = 17/39 (43%), Positives = 25/39 (64%)
Frame = +1
Query: 505 FAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVL 621
F A+DLD +G P F +A + ++D G + VTATD+ L
Sbjct: 133 FDAVDLDVFGTPIPFAQAAFRCVRD-GYVHVTATDLESL 170
>UniRef50_A5IYW7 Cluster: Putative uncharacterized protein; n=1;
Mycoplasma agalactiae|Rep: Putative uncharacterized
protein - Mycoplasma agalactiae
Length = 350
Score = 33.1 bits (72), Expect = 7.8
Identities = 26/86 (30%), Positives = 38/86 (44%), Gaps = 4/86 (4%)
Frame = +1
Query: 7 NEQNKCFYI-NNIFMRMETISNLKTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLF 183
NE +YI ++ M + N K+ + + E NP F D I + +
Sbjct: 178 NELGLAYYIFHDSGMAGINVENSSAYKQNKEATGMKKESRVLNPESNFFDDKKIYKVFVT 237
Query: 184 IEDYKAEK---LARFEKKQKKLETVQ 252
E Y+A+K L F KK KKL T+Q
Sbjct: 238 FESYQADKIKALISFCKKFKKLHTMQ 263
>UniRef50_Q9LVG3 Cluster: Similarity to unknown protein; n=6;
Arabidopsis thaliana|Rep: Similarity to unknown protein
- Arabidopsis thaliana (Mouse-ear cress)
Length = 327
Score = 33.1 bits (72), Expect = 7.8
Identities = 22/89 (24%), Positives = 42/89 (47%), Gaps = 4/89 (4%)
Frame = +1
Query: 505 FAAIDL--DPYGCPSI--FLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSL 672
+ A+DL D +GC ++ + A LL + A++ L+ ++ ++
Sbjct: 153 YHALDLACDQHGCIALNDIITDADDPYYRDQLLELVASNALRLSNDASGNFVVQHVLTLY 212
Query: 673 KTKCCHEMALRIMLQCIEQHANRYSRYIV 759
++C H +A+ + QCIE +Y YIV
Sbjct: 213 DSRCIHNIAVNLYGQCIELSFKKYGSYIV 241
>UniRef50_A0B5V5 Cluster: Putative RNA methylase, NOL1/NOP2/sun
family; n=1; Methanosaeta thermophila PT|Rep: Putative
RNA methylase, NOL1/NOP2/sun family - Methanosaeta
thermophila (strain DSM 6194 / PT)
(Methanothrixthermophila (strain DSM 6194 / PT))
Length = 302
Score = 33.1 bits (72), Expect = 7.8
Identities = 15/63 (23%), Positives = 34/63 (53%)
Frame = +1
Query: 271 NPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIET 450
+P+P IL+ +A G ++ + + ++ ++AND S + ++ N+E ++ T
Sbjct: 97 DPQPGERILDLCAAPGGKAAQISMQMSNKGLVVANDSSSARIVPLRANLERLGAVNVVVT 156
Query: 451 SHD 459
S+D
Sbjct: 157 SYD 159
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 642,813,613
Number of Sequences: 1657284
Number of extensions: 11569848
Number of successful extensions: 34352
Number of sequences better than 10.0: 120
Number of HSP's better than 10.0 without gapping: 32885
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 34231
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 63792713725
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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