Bombyx BLAST Result against bee

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt2a22
         (741 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY217747-1|AAP45005.1|  246|Apis mellifera short-chain dehydroge...    58   7e-11
DQ026034-1|AAY87893.1|  569|Apis mellifera nicotinic acetylcholi...    24   1.3  
DQ026033-1|AAY87892.1|  569|Apis mellifera nicotinic acetylcholi...    24   1.3  
DQ069332-1|AAZ32217.1|  296|Apis mellifera RNA polymerase II lar...    23   4.0  
AY500239-1|AAR92109.1|  555|Apis mellifera neuronal nicotinic ac...    21   9.2  

>AY217747-1|AAP45005.1|  246|Apis mellifera short-chain
           dehydrogenase/reductase protein.
          Length = 246

 Score = 58.4 bits (135), Expect = 7e-11
 Identities = 51/200 (25%), Positives = 92/200 (46%), Gaps = 6/200 (3%)
 Frame = +3

Query: 102 VGRLALVTGAGSGIGRAACQVLSKEGATVIAADRKYEAAVQTIKTHTALAPGTNGGEEHS 281
           +  +ALVTGA SGIG+   + L  +G  VI    + +  ++T+       PG     +  
Sbjct: 6   IDEVALVTGANSGIGKCLIECLVGKGMKVIGIAPQVD-KMKTLVEELKSKPG-----KLV 59

Query: 282 AVELDVSNSKAVQDLVQDTLKQYKKPPCIIVNSAGITRDNWMLKLSEEDYDSVLNVNLKG 461
            ++ D+SN   +  +++   K       I++N+A I  D  +      D+  + ++NL G
Sbjct: 60  PLQCDLSNQNDILKVIEWVEKNLGAID-ILINNATINIDVTLQNDEVLDWKKIFDINLLG 118

Query: 462 TFLVMQAFAKAMAESSV-TGSIINISSIVGKY---GNMGQTNYASSKAGVVAMTQSAAKE 629
              ++Q   K M +  +  G I+NI+   G      N  +  Y +SK  +  +T     E
Sbjct: 119 LTCMIQEVLKLMKKKGINNGIIVNINDASGLNLLPMNRNRPAYLASKCALTTLTDCLRSE 178

Query: 630 LG--KFNIRVNAILPGFIDT 683
           L   + NI+V +I P  ++T
Sbjct: 179 LAQCESNIKVISISPDLVET 198


>DQ026034-1|AAY87893.1|  569|Apis mellifera nicotinic acetylcholine
           receptor alpha4subunit protein.
          Length = 569

 Score = 24.2 bits (50), Expect = 1.3
 Identities = 17/58 (29%), Positives = 29/58 (50%)
 Frame = +3

Query: 297 VSNSKAVQDLVQDTLKQYKKPPCIIVNSAGITRDNWMLKLSEEDYDSVLNVNLKGTFL 470
           V+ +   + L  D L  Y K    +VN++ + R    LKLS+     +++VNLK   +
Sbjct: 23  VAGNPDAKRLYDDLLSNYNKLVRPVVNTSDVLRVCIKLKLSQ-----LIDVNLKNQIM 75


>DQ026033-1|AAY87892.1|  569|Apis mellifera nicotinic acetylcholine
           receptor alpha4subunit protein.
          Length = 569

 Score = 24.2 bits (50), Expect = 1.3
 Identities = 17/58 (29%), Positives = 29/58 (50%)
 Frame = +3

Query: 297 VSNSKAVQDLVQDTLKQYKKPPCIIVNSAGITRDNWMLKLSEEDYDSVLNVNLKGTFL 470
           V+ +   + L  D L  Y K    +VN++ + R    LKLS+     +++VNLK   +
Sbjct: 23  VAGNPDAKRLYDDLLSNYNKLVRPVVNTSDVLRVCIKLKLSQ-----LIDVNLKNQIM 75


>DQ069332-1|AAZ32217.1|  296|Apis mellifera RNA polymerase II large
           subunit protein.
          Length = 296

 Score = 22.6 bits (46), Expect = 4.0
 Identities = 11/23 (47%), Positives = 13/23 (56%)
 Frame = +3

Query: 489 KAMAESSVTGSIINISSIVGKYG 557
           KAM  S   GS INIS ++   G
Sbjct: 228 KAMVVSGSKGSNINISQVIACVG 250


>AY500239-1|AAR92109.1|  555|Apis mellifera neuronal nicotinic
           acetylcholine receptoralpha7-1 protein.
          Length = 555

 Score = 21.4 bits (43), Expect = 9.2
 Identities = 10/34 (29%), Positives = 18/34 (52%)
 Frame = -3

Query: 208 YFLSAAITVAPSFDKTWHAARPIPEPAPVTRARR 107
           Y+L   + ++   DK    A+   +P+PVT A +
Sbjct: 338 YWLPCILRMSRPSDKEEREAQKSQKPSPVTGASK 371


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 208,780
Number of Sequences: 438
Number of extensions: 4637
Number of successful extensions: 10
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 10
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 23144850
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)