Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt28n24
(374 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 171 4e-44
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 171 4e-44
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 171 4e-44
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 91 4e-20
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 89 3e-19
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 44 6e-06
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 36 0.002
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.002
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.002
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 36 0.002
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 32 0.026
SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces p... 29 0.18
SPCC63.03 |||DNAJ domain protein, DNAJC11 family|Schizosaccharom... 27 1.3
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 26 2.2
SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 25 2.9
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 3.9
SPBC1703.13c |||inorganic phosphate transporter |Schizosaccharom... 25 3.9
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 3.9
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 24 6.8
SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha... 24 6.8
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 24 6.8
SPAC926.09c |fas1||fatty acid synthase beta subunit Fas1|Schizos... 24 9.0
SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 24 9.0
SPBC12C2.11 ||SPBC21D10.02|glutamine-fructose-6-phosphate transa... 24 9.0
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 171 bits (415), Expect = 4e-44
Identities = 78/82 (95%), Positives = 80/82 (97%)
Frame = +2
Query: 62 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 241
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 242 DKLKAERERGITIDIALWKFET 307
DKLKAERERGITIDIALWKFET
Sbjct: 61 DKLKAERERGITIDIALWKFET 82
Score = 43.2 bits (97), Expect = 1e-05
Identities = 18/21 (85%), Positives = 19/21 (90%)
Frame = +3
Query: 312 KYYVTIIDAPGHRDFIXNMIT 374
KY VT+IDAPGHRDFI NMIT
Sbjct: 84 KYNVTVIDAPGHRDFIKNMIT 104
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 171 bits (415), Expect = 4e-44
Identities = 78/82 (95%), Positives = 80/82 (97%)
Frame = +2
Query: 62 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 241
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 242 DKLKAERERGITIDIALWKFET 307
DKLKAERERGITIDIALWKFET
Sbjct: 61 DKLKAERERGITIDIALWKFET 82
Score = 43.2 bits (97), Expect = 1e-05
Identities = 18/21 (85%), Positives = 19/21 (90%)
Frame = +3
Query: 312 KYYVTIIDAPGHRDFIXNMIT 374
KY VT+IDAPGHRDFI NMIT
Sbjct: 84 KYNVTVIDAPGHRDFIKNMIT 104
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 171 bits (415), Expect = 4e-44
Identities = 78/82 (95%), Positives = 80/82 (97%)
Frame = +2
Query: 62 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 241
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 242 DKLKAERERGITIDIALWKFET 307
DKLKAERERGITIDIALWKFET
Sbjct: 61 DKLKAERERGITIDIALWKFET 82
Score = 43.2 bits (97), Expect = 1e-05
Identities = 18/21 (85%), Positives = 19/21 (90%)
Frame = +3
Query: 312 KYYVTIIDAPGHRDFIXNMIT 374
KY VT+IDAPGHRDFI NMIT
Sbjct: 84 KYNVTVIDAPGHRDFIKNMIT 104
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 91.5 bits (217), Expect = 4e-20
Identities = 40/78 (51%), Positives = 56/78 (71%)
Frame = +2
Query: 74 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 253
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA+E GK S+ +W LD
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 254 AERERGITIDIALWKFET 307
ERE+G T+++ FET
Sbjct: 296 EEREKGKTVEVGRAYFET 313
Score = 27.1 bits (57), Expect = 0.96
Identities = 8/16 (50%), Positives = 14/16 (87%)
Frame = +3
Query: 324 TIIDAPGHRDFIXNMI 371
+++DAPGH+ ++ NMI
Sbjct: 319 SLLDAPGHKGYVTNMI 334
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 88.6 bits (210), Expect = 3e-19
Identities = 38/80 (47%), Positives = 56/80 (70%)
Frame = +2
Query: 74 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 253
K +++VV GHVDSGKST G ++++ G I+ R+++K EA GKGSF YAW+LD +
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234
Query: 254 AERERGITIDIALWKFETSQ 313
ER RG+T+D+A FE+ +
Sbjct: 235 EERARGVTMDVASTTFESDK 254
Score = 28.3 bits (60), Expect = 0.42
Identities = 11/13 (84%), Positives = 11/13 (84%)
Frame = +3
Query: 333 DAPGHRDFIXNMI 371
DAPGHRDFI MI
Sbjct: 261 DAPGHRDFISGMI 273
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 44.4 bits (100), Expect = 6e-06
Identities = 30/81 (37%), Positives = 42/81 (51%)
Frame = +2
Query: 68 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 247
++K H+NI IGHVD GK+T T I KC ++G+ SF +DK
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC--------------LSDLGQASFMDYSQIDK 93
Query: 248 LKAERERGITIDIALWKFETS 310
E+ RGITI A ++ET+
Sbjct: 94 APEEKARGITISSAHVEYETA 114
Score = 28.7 bits (61), Expect = 0.31
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3
Query: 330 IDAPGHRDFIXNMIT 374
+D PGH D+I NMIT
Sbjct: 121 VDCPGHADYIKNMIT 135
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 36.3 bits (80), Expect = 0.002
Identities = 24/68 (35%), Positives = 36/68 (52%)
Frame = +2
Query: 86 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 265
NI + H+DSGK+T T ++Y G I K+ E+ +G +D ++ ERE
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI---------KDIHEV-RGKDNVGAKMDFMELERE 110
Query: 266 RGITIDIA 289
+GITI A
Sbjct: 111 KGITIQSA 118
Score = 25.0 bits (52), Expect = 3.9
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = +3
Query: 315 YYVTIIDAPGHRDF 356
Y + IID PGH DF
Sbjct: 145 YNINIIDTPGHIDF 158
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.9 bits (79), Expect = 0.002
Identities = 28/73 (38%), Positives = 34/73 (46%)
Frame = +2
Query: 62 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 241
MGK N+ VI HVD GKST T L+ K G I A + G F +
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGII----------SAAKAGDARF-----M 57
Query: 242 DKLKAERERGITI 280
D E+ERG+TI
Sbjct: 58 DTRADEQERGVTI 70
Score = 23.8 bits (49), Expect = 9.0
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +3
Query: 315 YYVTIIDAPGHRDF 356
+ V +ID+PGH DF
Sbjct: 98 FLVNLIDSPGHVDF 111
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.9 bits (79), Expect = 0.002
Identities = 28/73 (38%), Positives = 34/73 (46%)
Frame = +2
Query: 62 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 241
MGK N+ VI HVD GKST T L+ K G I A + G F +
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGII----------SAAKAGDARF-----M 57
Query: 242 DKLKAERERGITI 280
D E+ERG+TI
Sbjct: 58 DTRADEQERGVTI 70
Score = 23.8 bits (49), Expect = 9.0
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +3
Query: 315 YYVTIIDAPGHRDF 356
+ V +ID+PGH DF
Sbjct: 98 FLVNLIDSPGHVDF 111
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 35.9 bits (79), Expect = 0.002
Identities = 25/72 (34%), Positives = 36/72 (50%)
Frame = +2
Query: 86 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 265
N+ +I H+D+GK+T T ++Y G F + G V+D L AER+
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG---------FTSHFGNVDTGD----TVMDYLPAERQ 76
Query: 266 RGITIDIALWKF 301
RGITI+ A F
Sbjct: 77 RGITINSAAISF 88
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 32.3 bits (70), Expect = 0.026
Identities = 26/82 (31%), Positives = 39/82 (47%)
Frame = +2
Query: 86 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 265
N VI H+D GKST + ++ G I++ + F LDKL+ ER
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINEHNF-----------RNQF-----LDKLEVERR 103
Query: 266 RGITIDIALWKFETSQVLCYHH 331
RGIT+ K +T ++ Y+H
Sbjct: 104 RGITV-----KAQTCSMIYYYH 120
Score = 23.8 bits (49), Expect = 9.0
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +3
Query: 315 YYVTIIDAPGHRDF 356
Y + +ID PGH DF
Sbjct: 124 YLLNLIDTPGHVDF 137
>SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 704
Score = 29.5 bits (63), Expect = 0.18
Identities = 14/43 (32%), Positives = 23/43 (53%)
Frame = -3
Query: 279 IVIPRSRSAFSLSNTQAYLKDPLPISWASFSNFSMVRLSIPPH 151
I IP+ A S+ N++ + +D LP + S V+L +P H
Sbjct: 171 IPIPKKNPARSVCNSKLFNEDTLPAEFEEVSISPPVKLELPTH 213
>SPCC63.03 |||DNAJ domain protein, DNAJC11
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 642
Score = 26.6 bits (56), Expect = 1.3
Identities = 16/44 (36%), Positives = 22/44 (50%)
Frame = -3
Query: 303 SNFQRAISIVIPRSRSAFSLSNTQAYLKDPLPISWASFSNFSMV 172
S F IS+ +P FSL+ ++ K LPI W S + S V
Sbjct: 403 STFGVNISVGVPTGSITFSLNWSRLGQKISLPIMWCSVFDRSAV 446
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 25.8 bits (54), Expect = 2.2
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = -1
Query: 269 HAHAQPLVCPIPKHI*RILYPFPGPPSRTSRWY 171
H+H PL P+P +L P P P S WY
Sbjct: 135 HSHHPPLHNPLPVSCQPVLRP-PPVPQVPSHWY 166
>SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 662
Score = 25.4 bits (53), Expect = 2.9
Identities = 15/43 (34%), Positives = 19/43 (44%)
Frame = -1
Query: 248 VCPIPKHI*RILYPFPGPPSRTSRWYVCQYHHICRSSDQWWWT 120
V +PK I I++ F P S S C+Y S D W T
Sbjct: 31 VLNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLSDDLSWRT 73
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 25.0 bits (52), Expect = 3.9
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +2
Query: 56 PKMGKEKTHINIVVIGHVDSGKST 127
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPBC1703.13c |||inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 311
Score = 25.0 bits (52), Expect = 3.9
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -1
Query: 203 PGPPSRTSRWYVCQYHHIC 147
P PP +T + Y QY+ +C
Sbjct: 8 PAPPKKTLQLYTPQYYGLC 26
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 25.0 bits (52), Expect = 3.9
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +3
Query: 315 YYVTIIDAPGHRDF 356
Y + +ID+PGH DF
Sbjct: 94 YLINLIDSPGHVDF 107
Score = 23.8 bits (49), Expect = 9.0
Identities = 10/29 (34%), Positives = 15/29 (51%)
Frame = +2
Query: 86 NIVVIGHVDSGKSTTTGHLIYKCGGIDKR 172
N ++ HVD GK+T L+ G I +
Sbjct: 21 NFTLLAHVDHGKTTLADSLLASNGIISSK 49
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 24.2 bits (50), Expect = 6.8
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = +3
Query: 321 VTIIDAPGHRDFIXN 365
+T+ID PG DFI N
Sbjct: 89 LTVIDTPGFGDFINN 103
>SPAC2G11.11c |prh1||ATP-dependent RNA helicase
Prh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 719
Score = 24.2 bits (50), Expect = 6.8
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = +2
Query: 89 IVVIGHVDSGKSTTTGHLIYKC 154
IVV+G SGKST + +C
Sbjct: 115 IVVVGETGSGKSTQIPQFLNEC 136
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 24.2 bits (50), Expect = 6.8
Identities = 8/22 (36%), Positives = 13/22 (59%)
Frame = +2
Query: 92 VVIGHVDSGKSTTTGHLIYKCG 157
+V+GH GK+T +Y+ G
Sbjct: 45 IVLGHTGCGKTTQIPQFLYEAG 66
>SPAC926.09c |fas1||fatty acid synthase beta subunit
Fas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2073
Score = 23.8 bits (49), Expect = 9.0
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = +2
Query: 68 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKF 187
K T +I+ I + DSGK+ T +Y+ G I +F
Sbjct: 1379 KVTTSASIIAILNNDSGKTVTVKGTVYRDGKEVIEVISRF 1418
>SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase
Prp16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1173
Score = 23.8 bits (49), Expect = 9.0
Identities = 8/23 (34%), Positives = 14/23 (60%)
Frame = +2
Query: 89 IVVIGHVDSGKSTTTGHLIYKCG 157
++V+G SGK+T +Y+ G
Sbjct: 511 LIVVGETGSGKTTQLAQFLYEDG 533
>SPBC12C2.11 ||SPBC21D10.02|glutamine-fructose-6-phosphate
transaminase |Schizosaccharomyces pombe|chr 2|||Manual
Length = 696
Score = 23.8 bits (49), Expect = 9.0
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = +2
Query: 98 IGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKY 229
I HV G+ L + GG RTIE E E + KG++ +
Sbjct: 297 IAHVRDGE-LHVHRLRREGGGSTTRTIETLEMEIASVMKGNYDH 339
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,423,679
Number of Sequences: 5004
Number of extensions: 25400
Number of successful extensions: 108
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 86
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 107
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 120195862
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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