Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt28n09
(531 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 147 8e-37
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 98 8e-22
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 93 3e-20
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 93 3e-20
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 83 3e-17
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 82 4e-17
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 74 2e-14
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 70 2e-13
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 70 3e-13
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 69 6e-13
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 66 2e-12
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 64 1e-11
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 61 9e-11
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 42 6e-05
SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosacchar... 29 0.43
SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting pro... 26 3.0
SPAC6C3.04 |cit1||citrate synthase|Schizosaccharomyces pombe|chr... 26 4.0
SPCP1E11.11 |||Puf family RNA-binding protein|Schizosaccharomyce... 26 4.0
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo... 26 4.0
SPAC24C9.05c |mug70||conserved protein |Schizosaccharomyces pomb... 26 4.0
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 26 4.0
SPBC646.06c |agn2||glucan endo-1,3-alpha-glucosidase Agn2|Schizo... 25 5.3
SPAC821.12 |orb6||serine/threonine protein kinase Orb6|Schizosac... 25 9.3
SPBC29A3.14c |trt1||telomerase reverse transcriptase 1 protein T... 25 9.3
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 147 bits (357), Expect = 8e-37
Identities = 70/131 (53%), Positives = 90/131 (68%), Gaps = 1/131 (0%)
Frame = +2
Query: 140 QNINPHYASSSNPAKQSEDTIKLKDNHAVSKRLQKELMELMRCSDKGISAFPESE-NLFK 316
QN NPH S K S D H+V+KRL+ ELM LM + GISAFP+S+ NL
Sbjct: 6 QNQNPHTNS-----KNSSSAGMAVDGHSVTKRLRSELMSLMMSNTPGISAFPDSDSNLLH 60
Query: 317 WIGTINGPLETVYAGHKYKLSLEFPNSYPYAPPVVKFMTPCFHPNVDTCGLICLDILKDK 496
W GTI GP +T Y G K+K+S+ FP +YPY+PP + F +P +HPNVD G ICLDILKDK
Sbjct: 61 WAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGNICLDILKDK 120
Query: 497 WTALYDVRTVL 529
W+A+Y+V+T+L
Sbjct: 121 WSAVYNVQTIL 131
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 97.9 bits (233), Expect = 8e-22
Identities = 43/100 (43%), Positives = 62/100 (62%)
Frame = +2
Query: 230 KRLQKELMELMRCSDKGISAFPESENLFKWIGTINGPLETVYAGHKYKLSLEFPNSYPYA 409
KR+ +EL +L + SA P ++LF W TI GP ++ YAG + LS+ FP YP+
Sbjct: 4 KRINRELADLGKDPPSSCSAGPVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFK 63
Query: 410 PPVVKFMTPCFHPNVDTCGLICLDILKDKWTALYDVRTVL 529
PP V F T +HPN+++ G ICLDIL+D+W+ + VL
Sbjct: 64 PPKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVL 103
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 92.7 bits (220), Expect = 3e-20
Identities = 45/103 (43%), Positives = 60/103 (58%)
Frame = +2
Query: 221 AVSKRLQKELMELMRCSDKGISAFPESENLFKWIGTINGPLETVYAGHKYKLSLEFPNSY 400
A+ KR+ KE+ L R GI A P +NL + T+ GP ++ Y G K+ L L P+ Y
Sbjct: 2 ALPKRIIKEIETLTRDPPPGIVAAPTEDNLRYFKITMEGPQQSAYEGGKFHLELFLPDEY 61
Query: 401 PYAPPVVKFMTPCFHPNVDTCGLICLDILKDKWTALYDVRTVL 529
P PP V+F+T +HPNVD G ICL LK W+ +RTVL
Sbjct: 62 PMMPPNVRFLTKIYHPNVDKLGRICLSTLKKDWSPALQIRTVL 104
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 92.7 bits (220), Expect = 3e-20
Identities = 40/100 (40%), Positives = 60/100 (60%)
Frame = +2
Query: 230 KRLQKELMELMRCSDKGISAFPESENLFKWIGTINGPLETVYAGHKYKLSLEFPNSYPYA 409
+RL ++ + + G+SA P S+N+ W I GP +T + +KL L F YP
Sbjct: 7 RRLMRDFKRMQQDPPAGVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNK 66
Query: 410 PPVVKFMTPCFHPNVDTCGLICLDILKDKWTALYDVRTVL 529
PP+VKF++ FHPNV G +CLDIL+++W+ YDV +L
Sbjct: 67 PPLVKFVSTMFHPNVYANGELCLDILQNRWSPTYDVAAIL 106
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 82.6 bits (195), Expect = 3e-17
Identities = 39/90 (43%), Positives = 53/90 (58%)
Frame = +2
Query: 227 SKRLQKELMELMRCSDKGISAFPESENLFKWIGTINGPLETVYAGHKYKLSLEFPNSYPY 406
S+RL KE +L I +NLF W T GP ++VYAG K+ SL+FP YP+
Sbjct: 9 SRRLTKEYSDLREHPIPDIRVNLVDDNLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPF 68
Query: 407 APPVVKFMTPCFHPNVDTCGLICLDILKDK 496
PP ++F T +HPN D+ G +CL ILK +
Sbjct: 69 QPPTIEFTTRIYHPNFDSEGNVCLAILKQQ 98
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 82.2 bits (194), Expect = 4e-17
Identities = 39/109 (35%), Positives = 63/109 (57%), Gaps = 1/109 (0%)
Frame = +2
Query: 206 LKDNHAVSKRLQKELMELMRCSDKGISAFPESENLFKWIGTINGPLETVYAGHKYKLSLE 385
+ DN S+R+ KEL ++ + GI + ++++ G GP T Y G + + +E
Sbjct: 1 MSDNR--SRRIAKELADVQQDKQAGIQVWTINDDISHLKGMFRGPEGTPYEGGYFVVDIE 58
Query: 386 FPNSYPYAPPVVKFMTPCFHPNVDT-CGLICLDILKDKWTALYDVRTVL 529
P YP+ PP + F T +HPNV + G ICLDILKD+W+ +Y +++ L
Sbjct: 59 IPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDILKDQWSPVYTMKSAL 107
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 73.7 bits (173), Expect = 2e-14
Identities = 37/87 (42%), Positives = 48/87 (55%), Gaps = 1/87 (1%)
Frame = +2
Query: 230 KRLQKELMELMRCSDKGISAFPESEN-LFKWIGTINGPLETVYAGHKYKLSLEFPNSYPY 406
+RL KE EL GI+A P +E+ F W I GP T + G Y +L+FP+ YP
Sbjct: 8 RRLMKEYKELTENGPDGITAGPSNEDDFFTWDCLIQGPDGTPFEGGLYPATLKFPSDYPL 67
Query: 407 APPVVKFMTPCFHPNVDTCGLICLDIL 487
PP +KF FHPNV G +C+ IL
Sbjct: 68 GPPTLKFECEFFHPNVYKDGTVCISIL 94
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 70.1 bits (164), Expect = 2e-13
Identities = 33/90 (36%), Positives = 52/90 (57%), Gaps = 1/90 (1%)
Frame = +2
Query: 221 AVSKRLQKELMELMRCSDKGISA-FPESENLFKWIGTINGPLETVYAGHKYKLSLEFPNS 397
A + L+K+L E+ + +G S + +++F+W I GP +T+Y G + +L FP
Sbjct: 5 ASEQLLRKQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQD 64
Query: 398 YPYAPPVVKFMTPCFHPNVDTCGLICLDIL 487
YP PP +KF T +HPNV G +C+ IL
Sbjct: 65 YPLMPPKMKFTTEIWHPNVHPNGEVCISIL 94
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 69.7 bits (163), Expect = 3e-13
Identities = 30/97 (30%), Positives = 61/97 (62%), Gaps = 1/97 (1%)
Frame = +2
Query: 230 KRLQKELMELMRCSDKGISAFPESENLFKWIGTINGPLETVYAGHKYKLSLEFPNSYPYA 409
+R++ ++M+L+ SD ++ ++N+ ++ +GP ET Y+G +K+ +E P+ YP+
Sbjct: 6 RRIETDVMKLLM-SDYEVTLV--NDNMQEFYVRFHGPSETPYSGGIWKVHVELPSEYPWK 62
Query: 410 PPVVKFMTPCFHPNVD-TCGLICLDILKDKWTALYDV 517
P + F+ FHPN+D G +CLD++ W+ ++D+
Sbjct: 63 SPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFDM 99
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 68.5 bits (160), Expect = 6e-13
Identities = 35/101 (34%), Positives = 52/101 (51%), Gaps = 3/101 (2%)
Frame = +2
Query: 230 KRLQKELMELMRCSDKGISAF-PESE-NLFKWIGTINGPLETVYAGHKYKLSLEFPNSYP 403
KRL KE G+ P ++ ++F W I GP ET Y G ++ L + YP
Sbjct: 6 KRLHKEYASFQAGDISGLLLLKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYP 65
Query: 404 YAPPVVKFMTPCFHPNVD-TCGLICLDILKDKWTALYDVRT 523
+PP V F T HPN+ T G +C+DILK W+ + +++
Sbjct: 66 ISPPSVYFQTKIVHPNISWTNGEVCMDILKTHWSPAWSLQS 106
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 66.5 bits (155), Expect = 2e-12
Identities = 36/102 (35%), Positives = 52/102 (50%), Gaps = 2/102 (1%)
Frame = +2
Query: 230 KRLQKELMELMRCSDKGISAFPESENLFKWIGTINGPLETVYAGHKYKLSLEFPNSYPYA 409
KRL KE + L + + + A P +EN+ +W I GP +T Y G +Y +L FP YP+
Sbjct: 8 KRLMKEYLALQKNPVELVDAKPATENILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFK 67
Query: 410 PPVVKFMTPC--FHPNVDTCGLICLDILKDKWTALYDVRTVL 529
PP ++ +TP F N C L D W + V T+L
Sbjct: 68 PPAIRMITPSGRFQTNTRLC-LSFSDFHPKSWNPSWMVSTIL 108
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 64.1 bits (149), Expect = 1e-11
Identities = 38/106 (35%), Positives = 53/106 (50%), Gaps = 7/106 (6%)
Frame = +2
Query: 233 RLQKELMELMRCSDKGISAFP--ESE---NLFKWIGTINGPLETVYAGHKYKLSLEFPNS 397
RLQ+E + R G A P S+ +L W I G +T + G YKL++ FP
Sbjct: 8 RLQEERKQWRRDHPFGFYAKPCKSSDGGLDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEE 67
Query: 398 YPYAPPVVKFMTPCFHPNVDTCGLICLDILKDK--WTALYDVRTVL 529
YP PP +F P FHPNV G +CL IL ++ W ++ +L
Sbjct: 68 YPTRPPKCRFTPPLFHPNVYPSGTVCLSILNEEEGWKPAITIKQIL 113
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 61.3 bits (142), Expect = 9e-11
Identities = 23/64 (35%), Positives = 40/64 (62%)
Frame = +2
Query: 338 PLETVYAGHKYKLSLEFPNSYPYAPPVVKFMTPCFHPNVDTCGLICLDILKDKWTALYDV 517
P E Y G K+K ++ ++YP+ PP VK + +HPN+D G +CL+IL+ W + ++
Sbjct: 60 PDEGYYKGGKFKFRIQIDDNYPHDPPKVKCLNKIYHPNIDIEGNVCLNILRQDWNPVLNL 119
Query: 518 RTVL 529
++L
Sbjct: 120 NSIL 123
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 41.9 bits (94), Expect = 6e-05
Identities = 27/78 (34%), Positives = 38/78 (48%), Gaps = 4/78 (5%)
Frame = +2
Query: 308 LFKWIGTINGPLETVYAGHKYKLSLEFPNSYPYAPPVVKFMTPCFHPNVD-TCGLI---C 475
L W TI GP +V+ Y L + +YP APP+V F++ P VD G +
Sbjct: 38 LSDWNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFVSRINLPGVDGETGKVNPHK 97
Query: 476 LDILKDKWTALYDVRTVL 529
+D L+ W Y + TVL
Sbjct: 98 IDCLR-HWKREYSMETVL 114
>SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 232
Score = 29.1 bits (62), Expect = 0.43
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -2
Query: 278 LYHCTALAPLALSGVVCSQHDCLLV*SCLHSA 183
LY C A + ++G VC CL V C H A
Sbjct: 181 LYECNACREIVIAGYVCDCGYCLHVYCCKHLA 212
>SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting protein
3 homolog Bud6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1385
Score = 26.2 bits (55), Expect = 3.0
Identities = 12/42 (28%), Positives = 23/42 (54%)
Frame = +2
Query: 140 QNINPHYASSSNPAKQSEDTIKLKDNHAVSKRLQKELMELMR 265
QNI+ ++ + S K SED KL +N +V + + + ++
Sbjct: 920 QNISTNHTNDSAALKSSEDHSKLANNFSVPDSIDHKFYQQVK 961
>SPAC6C3.04 |cit1||citrate synthase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 473
Score = 25.8 bits (54), Expect = 4.0
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +1
Query: 307 PLQMDRDHQWTFRN 348
P+QMD+DH + F N
Sbjct: 237 PIQMDKDHSYNFAN 250
>SPCP1E11.11 |||Puf family RNA-binding protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 642
Score = 25.8 bits (54), Expect = 4.0
Identities = 12/43 (27%), Positives = 21/43 (48%)
Frame = +2
Query: 161 ASSSNPAKQSEDTIKLKDNHAVSKRLQKELMELMRCSDKGISA 289
+ + N + S K++HA K+LQKE + +D + A
Sbjct: 91 SEAENDEESSSQKSNSKESHAQRKKLQKERKAMKPFADTSLKA 133
>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
Length = 457
Score = 25.8 bits (54), Expect = 4.0
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 371 KLSLEFPNSYPYAPPVVKFMTPCFHPNV 454
KLS+E+ N P AP +V CF +V
Sbjct: 112 KLSMEWDNIMPNAPGLVNLGNTCFMNSV 139
>SPAC24C9.05c |mug70||conserved protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 730
Score = 25.8 bits (54), Expect = 4.0
Identities = 16/51 (31%), Positives = 23/51 (45%)
Frame = +2
Query: 332 NGPLETVYAGHKYKLSLEFPNSYPYAPPVVKFMTPCFHPNVDTCGLICLDI 484
NG + V+ H L + PY V++ MTP HP+ C L L +
Sbjct: 296 NGAVSGVFTAHDVVLRVLAAGLDPYRSSVIRVMTP--HPD---CALASLRV 341
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 25.8 bits (54), Expect = 4.0
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = +2
Query: 365 KYKLSLEFPNSYPYAPPVVK 424
K L +E SYPY PV+K
Sbjct: 62 KLDLCIELGRSYPYVKPVIK 81
>SPBC646.06c |agn2||glucan endo-1,3-alpha-glucosidase
Agn2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 433
Score = 25.4 bits (53), Expect = 5.3
Identities = 16/65 (24%), Positives = 30/65 (46%), Gaps = 2/65 (3%)
Frame = +2
Query: 143 NINPHYASSSNPAKQSEDTIKLKDNHAVSKRLQKELMELM--RCSDKGISAFPESENLFK 316
N P+Y SS P +ED + ++++ A +K+ + + + K S F E +
Sbjct: 175 NCWPYYTSS--PTSDAEDLVYIQNSKATNKKYMATVSPIFYTHFTSKNYSFFSEGLWFTR 232
Query: 317 WIGTI 331
W+ I
Sbjct: 233 WMQLI 237
>SPAC821.12 |orb6||serine/threonine protein kinase
Orb6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 469
Score = 24.6 bits (51), Expect = 9.3
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -3
Query: 124 RKSNEHFLKIARDTNFSVNRRQLPVEFEIAT 32
+K EH+ K+A D N+R++ +E +AT
Sbjct: 28 KKYIEHYYKVAVDHAVERNQRRINLEQRLAT 58
>SPBC29A3.14c |trt1||telomerase reverse transcriptase 1 protein Trt1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 988
Score = 24.6 bits (51), Expect = 9.3
Identities = 15/48 (31%), Positives = 27/48 (56%), Gaps = 3/48 (6%)
Frame = -3
Query: 202 NRVFTLLSWVRRR---RVMWIDVLSHNILRKSNEHFLKIARDTNFSVN 68
N+VF L + R +++W + I+ K E FLK++R +FS++
Sbjct: 341 NQVFAFLRSILVRVFPKLIWGNQRIFEIILKDLETFLKLSRYESFSLH 388
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,227,301
Number of Sequences: 5004
Number of extensions: 46056
Number of successful extensions: 154
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 218398248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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