Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt28j21
         (508 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    87   1e-18
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    81   7e-17
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    57   2e-09
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    49   4e-07
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    44   1e-05
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    38   7e-04
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi...    32   0.043
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual    30   0.17 
SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Sc...    29   0.30 
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce...    29   0.30 
SPBC119.14 |rti1||Rad22 homolog Rti1|Schizosaccharomyces pombe|c...    28   0.92 
SPBP23A10.04 |apc2||anaphase-promoting complex subunit Apc2 |Sch...    26   3.7  
SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex lar...    26   3.7  
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po...    25   6.5  

>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 87.4 bits (207), Expect = 1e-18
 Identities = 44/118 (37%), Positives = 66/118 (55%)
 Frame = +2

Query: 152 SSRKTAGRRINKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFIDKDDLHDMLAS 331
           SS  T+ +R+  + A+RA+S  FA    +QI E KEAF ++D++ DG I ++D+  ML S
Sbjct: 17  SSNTTSSQRVAAQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTS 76

Query: 332 LGKNPTEDYLEGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENNGVI 505
           L ++ +ED +  M      PIN   FLT  G  L    P + +  AF  FD+  +G I
Sbjct: 77  LNQDASEDSINHMFESINPPINLAAFLTAMGSMLCRISPRNDLLEAFSTFDDTQSGKI 134


>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 81.4 bits (192), Expect = 7e-17
 Identities = 39/93 (41%), Positives = 63/93 (67%), Gaps = 4/93 (4%)
 Frame = +2

Query: 239 QIAEFKEAFNMIDQNRDGFIDKDDLHDMLASLGKNPTEDYLEGMMNEAP----GPINFTM 406
           QIAEF+EAF++ D+++DG I  ++L  ++ SLG++PT   L+ M+NE      G I+FT 
Sbjct: 10  QIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTE 69

Query: 407 FLTLFGERLQGTDPEDVIKNAFGCFDEENNGVI 505
           FLT+   +++ TD E+ ++ AF  FD++ NG I
Sbjct: 70  FLTMMARKMKDTDNEEEVREAFKVFDKDGNGYI 102



 Score = 36.7 bits (81), Expect = 0.002
 Identities = 21/66 (31%), Positives = 37/66 (56%), Gaps = 4/66 (6%)
 Frame = +2

Query: 224 MFDQAQIAEFKEAFNMIDQNRDGFIDKDDLHDMLASLGKNPTEDYLEGMMNEA----PGP 391
           M D     E +EAF + D++ +G+I  ++L  +L SLG+  +++ +  M+ EA     G 
Sbjct: 78  MKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGV 137

Query: 392 INFTMF 409
           IN+  F
Sbjct: 138 INYEEF 143


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 56.8 bits (131), Expect = 2e-09
 Identities = 31/89 (34%), Positives = 48/89 (53%)
 Frame = +2

Query: 239 QIAEFKEAFNMIDQNRDGFIDKDDLHDMLASLGKNPTEDYLEGMMNEAPGPINFTMFLTL 418
           Q  E KEAF + D ++DG I    +  +L SLG N T+  L  + NE    I+   F++ 
Sbjct: 7   QTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSF 66

Query: 419 FGERLQGTDPEDVIKNAFGCFDEENNGVI 505
              +L+ T+ E+    AF  FD++N+G I
Sbjct: 67  VSNKLRETESEEEYIKAFRVFDKDNSGYI 95


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 49.2 bits (112), Expect = 4e-07
 Identities = 28/90 (31%), Positives = 50/90 (55%), Gaps = 4/90 (4%)
 Frame = +2

Query: 251 FKEAFNMIDQNRDGFIDKDDLHDMLASLGKNPTEDYLEGMMNEAPGPINFTMFLTLF--- 421
           +K+AF++ D++  G I K  + D+L + G+NPT   +  + +  P  ++   FL +    
Sbjct: 8   YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVDMEQFLQVLNRP 67

Query: 422 -GERLQGTDPEDVIKNAFGCFDEENNGVIG 508
            G  + G DPE+ +K  F  FD++  G+IG
Sbjct: 68  NGFDMPG-DPEEFVK-GFQVFDKDATGMIG 95


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 44.0 bits (99), Expect = 1e-05
 Identities = 25/95 (26%), Positives = 46/95 (48%), Gaps = 4/95 (4%)
 Frame = +2

Query: 233 QAQIAEFKEAFNMIDQNRDGFIDKDDLHDMLASLGKNPTEDYLEGMMNE----APGPINF 400
           + Q  +  EAF + D ++D  ID  +L   + +LG N  +  +  ++ +      G +  
Sbjct: 33  EEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQM 92

Query: 401 TMFLTLFGERLQGTDPEDVIKNAFGCFDEENNGVI 505
             F+ +  E++   DP + IK AF  FD++  G I
Sbjct: 93  EDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKI 127


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 38.3 bits (85), Expect = 7e-04
 Identities = 18/45 (40%), Positives = 29/45 (64%)
 Frame = +2

Query: 248 EFKEAFNMIDQNRDGFIDKDDLHDMLASLGKNPTEDYLEGMMNEA 382
           E +EAF++ D    G+ID +DL    A LG+N T++ L+ M++ A
Sbjct: 13  EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLA 57


>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
           Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 190

 Score = 32.3 bits (70), Expect = 0.043
 Identities = 18/73 (24%), Positives = 33/73 (45%), Gaps = 3/73 (4%)
 Frame = +2

Query: 296 IDKDDLHDMLAS--LGKNPTEDYLEGMMNEAP-GPINFTMFLTLFGERLQGTDPEDVIKN 466
           + +D L D++ S    K   + + +G   + P G +N + F  ++ +     DP    + 
Sbjct: 8   LSQDQLQDLVRSTRFDKKELQQWYKGFFKDCPSGHLNKSEFQKIYKQFFPFGDPSAFAEY 67

Query: 467 AFGCFDEENNGVI 505
            F  FD + NG I
Sbjct: 68  VFNVFDADKNGYI 80



 Score = 29.1 bits (62), Expect = 0.40
 Identities = 16/61 (26%), Positives = 28/61 (45%)
 Frame = +2

Query: 179 INKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFIDKDDLHDMLASLGKNPTEDY 358
           +NK   Q+     F   D +  AE+   FN+ D +++G+ID  +    L+   +    D 
Sbjct: 43  LNKSEFQKIYKQFFPFGDPSAFAEY--VFNVFDADKNGYIDFKEFICALSVTSRGELNDK 100

Query: 359 L 361
           L
Sbjct: 101 L 101


>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1236

 Score = 30.3 bits (65), Expect = 0.17
 Identities = 24/73 (32%), Positives = 35/73 (47%), Gaps = 1/73 (1%)
 Frame = -2

Query: 501 TPLFSSSKHPNAFLMTSSGSVPCSLSPNRVRNIVKLIGPGASFIIPSK*SSV-GFLPKDA 325
           TP  S    P   +++SS S+P S S +   +I  +    +S  IPS  SSV   L    
Sbjct: 525 TPSSSIISSPMTSVLSSSSSIPTSSSSDFSSSITTISSGISSSSIPSTFSSVSSILSSST 584

Query: 324 SISCRSSLSMNPS 286
           S    +SLS++ S
Sbjct: 585 SSPSSTSLSISSS 597


>SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit
            Apc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1458

 Score = 29.5 bits (63), Expect = 0.30
 Identities = 12/43 (27%), Positives = 24/43 (55%), Gaps = 2/43 (4%)
 Frame = -1

Query: 283  VLINHIESLLELCYLSLIKHSKHIGGCTL--CALLVNATTGCL 161
            +LIN ++S + LC+L    H + +   T+  C  +V  ++ C+
Sbjct: 1119 ILINFLDSFIRLCHLPAKTHDERVTAVTVIRCTQIVALSSSCV 1161


>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 174

 Score = 29.5 bits (63), Expect = 0.30
 Identities = 15/45 (33%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
 Frame = +2

Query: 248 EFKEAFNMIDQNRDGFIDKDDLHDMLASL-GKNPTEDYLEGMMNE 379
           + K AF + D +RDG+I   +L+ +L  + G N  ED L+ ++++
Sbjct: 94  KLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDK 138


>SPBC119.14 |rti1||Rad22 homolog Rti1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 371

 Score = 27.9 bits (59), Expect = 0.92
 Identities = 14/56 (25%), Positives = 26/56 (46%), Gaps = 2/56 (3%)
 Frame = +2

Query: 230 DQAQIAEFKEAFNMIDQNRDGFIDKDDLHDML--ASLGKNPTEDYLEGMMNEAPGP 391
           +  ++ EF+E  N  + +    +DK D HD +  A + K P   ++   +  A  P
Sbjct: 267 ESPRVEEFEELLNQFEGDEKVSVDKIDAHDKMTEAQVVKIPPVQFMNARVAAAENP 322


>SPBP23A10.04 |apc2||anaphase-promoting complex subunit Apc2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 681

 Score = 25.8 bits (54), Expect = 3.7
 Identities = 12/31 (38%), Positives = 18/31 (58%)
 Frame = -1

Query: 478 APKCIFDDILRVSALQSLTKQSEKHRKVNWS 386
           A + + D+I  VSA+QS  + S    +V WS
Sbjct: 590 ATETVLDEIQGVSAVQSEAESSAAEMRVYWS 620


>SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex large
           subunit Nuc1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1689

 Score = 25.8 bits (54), Expect = 3.7
 Identities = 10/26 (38%), Positives = 16/26 (61%)
 Frame = +1

Query: 16  IYSLHKSTILICRHTRLNKFALSFLF 93
           +Y+L +ST L C H +L+K  +   F
Sbjct: 95  MYNLLRSTCLYCHHFKLSKVKVHLFF 120


>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 646

 Score = 25.0 bits (52), Expect = 6.5
 Identities = 15/48 (31%), Positives = 26/48 (54%)
 Frame = -1

Query: 280 LINHIESLLELCYLSLIKHSKHIGGCTLCALLVNATTGCLAR*HGEFY 137
           L+N I++   + + + + HS  +  C  C LLV+A+ G  A+    FY
Sbjct: 125 LLNLIDTPGHVDFRAEVMHS--LAACEGCILLVDASQGIQAQTLSNFY 170


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,101,528
Number of Sequences: 5004
Number of extensions: 41463
Number of successful extensions: 118
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 117
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 202220600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)