Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt28h24
(481 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 198 3e-52
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 107 1e-24
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 91 1e-19
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 85 4e-18
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 76 2e-15
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 58 9e-10
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 41 1e-04
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 36 0.003
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 36 0.004
SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccha... 28 0.64
SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos... 28 0.64
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc... 28 0.84
SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ... 27 1.9
SPBC4B4.08 |ght2||hexose transporter Ght2 |Schizosaccharomyces p... 26 2.6
SPCC548.07c |ght1||hexose transporter Ght1 |Schizosaccharomyces ... 26 2.6
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 26 2.6
SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr 2||... 26 2.6
SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy... 26 3.4
SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein Usp104|Schi... 25 5.9
SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|ch... 25 7.8
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 198 bits (484), Expect = 3e-52
Identities = 92/119 (77%), Positives = 106/119 (89%)
Frame = +1
Query: 121 MAADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDAD 300
M LT+EQIAEF+EAFSLFD+D DG IT+ ELG VMRSLGQ+PT AELQDMINEVDAD
Sbjct: 1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDAD 60
Query: 301 GNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLT 477
GNGTIDF EFLTMMARKMKDTD+EEE+REAF+VFDKDGNG+I+ EL HV+T+LGE+L+
Sbjct: 61 GNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLS 119
Score = 71.3 bits (167), Expect = 7e-14
Identities = 33/80 (41%), Positives = 51/80 (63%)
Frame = +1
Query: 112 MVVMAADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEV 291
+ +MA + E +EAF +FDKDG+G IT +EL V+ SLG+ ++ E+ DMI E
Sbjct: 71 LTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA 130
Query: 292 DADGNGTIDFPEFLTMMARK 351
D DG+G I++ EF +++ K
Sbjct: 131 DTDGDGVINYEEFSRVISSK 150
Score = 57.2 bits (132), Expect = 1e-09
Identities = 34/103 (33%), Positives = 54/103 (52%), Gaps = 1/103 (0%)
Frame = +1
Query: 154 AEFKEAFSLFDKDGDGTITTKELGTVM-RSLGQNPTEAELQDMINEVDADGNGTIDFPEF 330
AE ++ + D DG+GTI E T+M R + E E+++ D DGNG I E
Sbjct: 48 AELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEE- 106
Query: 331 LTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTN 459
LT + + + S+EE+ + R D DG+G I+ E V+++
Sbjct: 107 LTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVISS 149
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 107 bits (256), Expect = 1e-24
Identities = 54/114 (47%), Positives = 77/114 (67%)
Frame = +1
Query: 139 TEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTID 318
++EQ E KEAF L+D D DG I T +G+V+RSLG N T+AEL + NE+ ID
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----GDAID 59
Query: 319 FPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTD 480
+F++ ++ K+++T+SEEE +AFRVFDKD +G+I A+ M LGEKL+D
Sbjct: 60 EKKFMSFVSNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSD 113
Score = 51.2 bits (117), Expect = 8e-08
Identities = 23/69 (33%), Positives = 40/69 (57%)
Frame = +1
Query: 145 EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFP 324
E E+ +AF +FDKD G I T + M++LG+ ++ E+Q M+ E D +G+ D+
Sbjct: 75 ESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYY 134
Query: 325 EFLTMMARK 351
+F+ + K
Sbjct: 135 DFVQRIMAK 143
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 90.6 bits (215), Expect = 1e-19
Identities = 45/116 (38%), Positives = 64/116 (55%)
Frame = +1
Query: 133 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGT 312
++TEEQ + EAF LFD D D I EL MR+LG N ++E+ ++ + D G G
Sbjct: 30 EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGY 89
Query: 313 IDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTD 480
+ +F+ +M K+ + D EEI+ AF +FD D G IS LR V L E + D
Sbjct: 90 LQMEDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDD 145
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 85.4 bits (202), Expect = 4e-18
Identities = 45/109 (41%), Positives = 66/109 (60%), Gaps = 2/109 (1%)
Frame = +1
Query: 160 FKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTM 339
+K+AFSLFD+ G G I +G ++R+ GQNPT AE I E+++ +D +FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAE----ITEIESTLPAEVDMEQFLQV 63
Query: 340 MARK--MKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTD 480
+ R EE + F+VFDKD G I ELR+V+T+LGEKL++
Sbjct: 64 LNRPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSN 112
Score = 41.9 bits (94), Expect = 5e-05
Identities = 20/62 (32%), Positives = 36/62 (58%)
Frame = +1
Query: 157 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLT 336
EF + F +FDKD G I EL V+ SLG+ + E+ +++ V +G +++ +F+
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVK-DGMVNYHDFVQ 136
Query: 337 MM 342
M+
Sbjct: 137 MI 138
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 76.2 bits (179), Expect = 2e-15
Identities = 41/114 (35%), Positives = 64/114 (56%)
Frame = +1
Query: 133 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGT 312
QLT QI E KEAF+L DKDGDG I +++ T++ SL Q+ +E D IN + N
Sbjct: 41 QLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASE----DSINHMFESINPP 96
Query: 313 IDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKL 474
I+ FLT M + ++ EAF FD +G I + +R ++++G+++
Sbjct: 97 INLAAFLTAMGSMLCRISPRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRM 150
Score = 33.9 bits (74), Expect = 0.013
Identities = 12/36 (33%), Positives = 26/36 (72%)
Frame = +1
Query: 373 EEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTD 480
+E++EAF + DKDG+G I +++ ++T+L + ++
Sbjct: 48 QELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASE 83
Score = 25.8 bits (54), Expect = 3.4
Identities = 12/46 (26%), Positives = 20/46 (43%)
Frame = +1
Query: 166 EAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG 303
EAFS FD G I + + S+G E++ ++ + G
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSYTSHG 166
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 57.6 bits (133), Expect = 9e-10
Identities = 32/112 (28%), Positives = 60/112 (53%)
Frame = +1
Query: 118 VMAADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA 297
+++ + E+I ++ F D + G+I E ++ S+ NP + L + VD
Sbjct: 12 LISNSSFSNEEIERIRKRFIKIDANQSGSIDRNEFLSIP-SVASNPLASRL---FSVVDE 67
Query: 298 DGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVM 453
DG G +DF EF+ ++ + EE+++ AF+++D D +G+IS EL V+
Sbjct: 68 DGGGDVDFQEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVL 119
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 40.7 bits (91), Expect = 1e-04
Identities = 20/59 (33%), Positives = 35/59 (59%)
Frame = +1
Query: 277 MINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVM 453
+ N DAD NG IDF EF+ ++ + + +++ AF+++D D NG IS E+ ++
Sbjct: 68 VFNVFDADKNGYIDFKEFICALSVTSRG-ELNDKLIWAFQLYDLDNNGLISYDEMLRIV 125
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 35.9 bits (79), Expect = 0.003
Identities = 18/46 (39%), Positives = 24/46 (52%)
Frame = +1
Query: 340 MARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLT 477
M+ K + +EE EAF +FD G+I +LR LGE LT
Sbjct: 1 MSVSTKRLEMDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLT 46
Score = 34.7 bits (76), Expect = 0.007
Identities = 23/62 (37%), Positives = 32/62 (51%)
Frame = +1
Query: 145 EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFP 324
E E +EAF LFD G I ++L LG+N T+ +LQ M++ A NG +
Sbjct: 9 EMDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDL--AGTNGKVSRE 66
Query: 325 EF 330
EF
Sbjct: 67 EF 68
>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 614
Score = 35.5 bits (78), Expect = 0.004
Identities = 19/51 (37%), Positives = 28/51 (54%)
Frame = +1
Query: 268 LQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNG 420
L D N++D DG G +D P T+ A + S +E+REA R + D +G
Sbjct: 21 LTDQFNKLDVDGKGYLDQP--TTIKAFEDSKKGSYDEVREAIREVNVDSSG 69
>SPCC1183.11 ||SPCC31H12.01|MS ion channel protein
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1011
Score = 28.3 bits (60), Expect = 0.64
Identities = 15/56 (26%), Positives = 29/56 (51%), Gaps = 2/56 (3%)
Frame = +1
Query: 130 DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLG--QNPTEAELQDMINEV 291
D+ E A + +S+FD+D +G IT +E+ +G + A L+D+ + +
Sbjct: 538 DETGEVDNATLEACYSIFDRDLNGDITCEEIELACVEIGKERKSISASLRDLNDSI 593
>SPAC694.06c |mrc1||mediator of replication checkpoint 1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1019
Score = 28.3 bits (60), Expect = 0.64
Identities = 12/43 (27%), Positives = 24/43 (55%)
Frame = +1
Query: 280 INEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDK 408
I+E D + NG + F + + +KD+ EE+ ++F + D+
Sbjct: 545 ISESDDESNGDMIRDSFDRLSSESIKDSQKTEELHDSFGINDE 587
>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 767
Score = 27.9 bits (59), Expect = 0.84
Identities = 13/55 (23%), Positives = 25/55 (45%)
Frame = +1
Query: 112 MVVMAADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQD 276
+ ++AAD L + + F + F++ DG+ T TT + + E + D
Sbjct: 12 VTIVAADGLYKRDVFRFPDPFAVLTVDGEQTHTTTAIKKTLNPYWNETFEVNVTD 66
>SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor,
zf-fungal binuclear cluster type |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 977
Score = 26.6 bits (56), Expect = 1.9
Identities = 9/27 (33%), Positives = 15/27 (55%)
Frame = -1
Query: 274 LEVRLLWGSVLATSSRCPALWS*WCHR 194
LE++ WGS+ A + +W+ W R
Sbjct: 850 LEMKNYWGSISALCDKMSEIWADWVQR 876
>SPBC4B4.08 |ght2||hexose transporter Ght2 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 531
Score = 26.2 bits (55), Expect = 2.6
Identities = 14/31 (45%), Positives = 18/31 (58%)
Frame = -2
Query: 471 FLSEVGHDVAQLGRGDEAVAVLVEDAEGLAD 379
FL E + Q+G+ +EAV VL E AE D
Sbjct: 202 FLPESPRYLIQVGKDEEAVRVLSESAELFPD 232
>SPCC548.07c |ght1||hexose transporter Ght1 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 557
Score = 26.2 bits (55), Expect = 2.6
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = -2
Query: 471 FLSEVGHDVAQLGRGDEAVAVLVEDAE 391
FLSE + +G+ DEA+ V+ ++AE
Sbjct: 198 FLSESPRYLVSIGKDDEAIQVMCKNAE 224
>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 630
Score = 26.2 bits (55), Expect = 2.6
Identities = 19/85 (22%), Positives = 31/85 (36%)
Frame = +1
Query: 166 EAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMA 345
+ F FD+D DG + +EL + R P N + +G + + +L +
Sbjct: 311 DLFYQFDRDNDGALNNEELSALFRHTPGLPEIWVSSQFPNSTVLNEHGYVTYNGWLAQWS 370
Query: 346 RKMKDTDSEEEIREAFRVFDKDGNG 420
A+ FD DG G
Sbjct: 371 MITLFDYKTTLAYLAYLGFDTDGRG 395
>SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1157
Score = 26.2 bits (55), Expect = 2.6
Identities = 16/70 (22%), Positives = 34/70 (48%), Gaps = 1/70 (1%)
Frame = +1
Query: 202 TITTKELGTVMRSLGQ-NPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEE 378
T + GT+ S+G + E + ++++ T DFPE L+ +++ EE
Sbjct: 545 TTSLHPFGTLYESIGTIGDPKVEYKAILHDFSCH---TYDFPESLSHCVKRLPTPIPAEE 601
Query: 379 IREAFRVFDK 408
+++ + + DK
Sbjct: 602 LQKRYNLRDK 611
>SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 786
Score = 25.8 bits (54), Expect = 3.4
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +1
Query: 118 VMAADQLTEEQIAEFKEAFSLFDKDGDGTITTK 216
++AAD L++ + + F++ DG+ T TTK
Sbjct: 12 IVAADGLSKRDLFRQPDPFAILTVDGEQTHTTK 44
>SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein
Usp104|Schizosaccharomyces pombe|chr 1|||Manual
Length = 695
Score = 25.0 bits (52), Expect = 5.9
Identities = 9/38 (23%), Positives = 19/38 (50%)
Frame = +1
Query: 274 DMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIRE 387
D + + + T + PE + ++ K+KD + E + E
Sbjct: 479 DCLEVLQISVDDTSNIPEIIARLSEKLKDREESEAVTE 516
>SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|chr
1|||Manual
Length = 783
Score = 24.6 bits (51), Expect = 7.8
Identities = 20/63 (31%), Positives = 28/63 (44%), Gaps = 2/63 (3%)
Frame = +1
Query: 259 EAELQDMINEVDADGNGTIDFPEFLT--MMARKMKDTDSEEEIREAFRVFDKDGNGFISA 432
E L+ +N + + N ++D P FL MM + + EIR A FD G F
Sbjct: 281 ETLLRYRLNLIMSFQNPSVD-PGFLCLLMMIFALGSMFAHMEIRSASNPFDNPGKQFFDT 339
Query: 433 AEL 441
A L
Sbjct: 340 ARL 342
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,744,203
Number of Sequences: 5004
Number of extensions: 31890
Number of successful extensions: 152
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 184020746
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -