Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt28h10
(493 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1672.05c |||tyrosine-tRNA ligase |Schizosaccharomyces pombe|... 159 3e-40
SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar... 27 1.5
SPCC622.11 |||LMBR1-like membrane protein|Schizosaccharomyces po... 26 2.7
SPAC343.19 ||SPAC824.01|phosphatidylinositol 4-kinase Lsb6 |Schi... 26 2.7
SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha... 26 3.5
SPBC16G5.08 |trp4||phosphoribosylanthranilate transferase Trp4|S... 26 3.5
SPAC26H5.06 |pot1||telomere end-binding protein Pot1 |Schizosacc... 25 4.7
SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 6.2
SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 8.2
>SPCC1672.05c |||tyrosine-tRNA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 401
Score = 159 bits (385), Expect = 3e-40
Identities = 71/115 (61%), Positives = 90/115 (78%)
Frame = +3
Query: 147 TWQEKKHLITRNLQEVLGDDKLTEILKQRDLKIYWGTATTGRPHVAYFVPMLKIADFLKA 326
T EK LITRNLQEVLG + EIL +RD+ +YWG+A TGRPH YFVPM+K+ADFLKA
Sbjct: 4 TPDEKYELITRNLQEVLGAKMIREILNERDISLYWGSAPTGRPHCGYFVPMMKLADFLKA 63
Query: 327 GCEVTILFADLHAYLDNMKAPWELLALRTQYYEAAIKAMLTSIGVPLEKLKFVRG 491
G VT+L AD+HA+LDNMKAP EL+ R +YYE +K++L S+ +P+EKL+FV G
Sbjct: 64 GVHVTVLLADVHAFLDNMKAPMELVQHRVRYYEIIVKSILKSLNIPIEKLRFVIG 118
>SPAC23A1.04c |mnl1||alpha mannosidase-like
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 787
Score = 27.1 bits (57), Expect = 1.5
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +3
Query: 393 ELLALRTQYYEAAIKAMLTSIGVPLEKLKFVRG 491
ELL L T+ E + A T G+P ++ ++G
Sbjct: 161 ELLTLATELAERLLPAFRTPTGIPFARINLMKG 193
>SPCC622.11 |||LMBR1-like membrane protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 562
Score = 26.2 bits (55), Expect = 2.7
Identities = 18/74 (24%), Positives = 30/74 (40%)
Frame = -1
Query: 268 PVVAVPQYIFKSLCFKISVSLSSPKTSCKFRVIKCFFSCQVAMVNLWRSTYLKYSRYCIY 89
PVVA Y++ F+I S+ S V + F Q ++V + + S I+
Sbjct: 336 PVVAYAFYVWILPAFRILFSIFMASMSVIIVVSEVFLHTQYSLVGIILQKFTNSSATSIF 395
Query: 88 FCHLYFYLRN*ISY 47
L+ Y +Y
Sbjct: 396 VSFLFVYYMRYCTY 409
>SPAC343.19 ||SPAC824.01|phosphatidylinositol 4-kinase Lsb6
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 624
Score = 26.2 bits (55), Expect = 2.7
Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
Frame = +3
Query: 99 YLEYFKYVDLHKLTMATWQEKKHLITRNLQEVLGDD-KLTEILKQRDLKIYWGTA 260
+ E+ + + LHKLT W EK ++ +L+ V D E + R L + G A
Sbjct: 443 FTEFTRQLFLHKLTSREWWEK---LSDDLRNVFNQDLDFDEKMFSRQLSLVKGQA 494
>SPAC16C9.06c |upf1||ATP-dependent RNA helicase
Upf1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 925
Score = 25.8 bits (54), Expect = 3.5
Identities = 17/65 (26%), Positives = 29/65 (44%), Gaps = 1/65 (1%)
Frame = +3
Query: 201 DDKLTEILKQRDLKIYWGTATTGRPHVAYFVPMLKIADF-LKAGCEVTILFADLHAYLDN 377
D +L E Q+D+ + W A R + +P L+ + L G E+ + Y
Sbjct: 238 DKRLKESQTQKDVVVRWDQAINKRYTAWFLLPKLESGEIRLAIGDEMKL------TYEGE 291
Query: 378 MKAPW 392
++APW
Sbjct: 292 LRAPW 296
>SPBC16G5.08 |trp4||phosphoribosylanthranilate transferase
Trp4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 354
Score = 25.8 bits (54), Expect = 3.5
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +3
Query: 402 ALRTQYYEAAIKAMLTSIGVPLEKLKFV 485
A+ + YEAA++A+LT P+E F+
Sbjct: 22 AIPLENYEAALRAILTGKASPVETASFL 49
>SPAC26H5.06 |pot1||telomere end-binding protein Pot1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 555
Score = 25.4 bits (53), Expect = 4.7
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = -1
Query: 130 WRSTYLKYSRYCIYFCHLYFYLRN*ISYHHVCCMINLRNWIN 5
WR + ++S CI + FY RN I M N+R I+
Sbjct: 254 WRGPFGRFSIRCILWDEHDFYCRNYIKEGDYVVMKNVRTKID 295
>SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 689
Score = 25.0 bits (52), Expect = 6.2
Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 3/39 (7%)
Frame = +3
Query: 144 ATWQEKKH---LITRNLQEVLGDDKLTEILKQRDLKIYW 251
+TWQ+KKH T + + + KLT I+ IYW
Sbjct: 62 STWQKKKHDDLTATVPVIDFRKNQKLTSIVVNAIQAIYW 100
>SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 867
Score = 24.6 bits (51), Expect = 8.2
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +3
Query: 300 LKIADFLKAGCEVTIL 347
LK++DFLKAG T++
Sbjct: 834 LKVSDFLKAGIPATLI 849
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,946,730
Number of Sequences: 5004
Number of extensions: 39165
Number of successful extensions: 90
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 87
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 90
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 192109570
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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