BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt28f20
(526 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyc... 30 0.24
SPBC1703.02 |rsc9||RSC complex subunit Rsc9|Schizosaccharomyces ... 27 2.3
SPBC14C8.17c |||SAGA complex subunit Spt8 |Schizosaccharomyces p... 27 2.3
SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi... 26 4.0
SPAC869.03c |||urea transporter |Schizosaccharomyces pombe|chr 1... 26 4.0
SPAC22A12.03c |csn4||COP9/signalosome complex subunit Csn4 |Schi... 26 4.0
SPCC330.07c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 6.9
SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Sch... 25 9.1
SPBC4B4.01c |||fumble family pantothenate kinase |Schizosaccharo... 25 9.1
SPCC16A11.03c |||DUF2009 protein|Schizosaccharomyces pombe|chr 3... 25 9.1
SPCC1682.03c |mug174||meiotically upregulated gene Mug174|Schizo... 25 9.1
>SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 807
Score = 29.9 bits (64), Expect = 0.24
Identities = 18/55 (32%), Positives = 26/55 (47%)
Frame = -3
Query: 359 KWLNLKTILPMLPSIGSGVTLLISCLLK*EYVCFRYSKRAKPITNVHMMMIILPV 195
+WL L T P G L ++C Y+ F YS R+K I HM I++ +
Sbjct: 479 EWLLLLTRKVFSPEFG----LFVNCEESSNYLWFNYSHRSKEIDYYHMSGILMGI 529
>SPBC1703.02 |rsc9||RSC complex subunit Rsc9|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 780
Score = 26.6 bits (56), Expect = 2.3
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = -3
Query: 92 RCCFSPINLSVFVITELLRLTCAHMRR 12
RCCF P + + T++ +L C+ M R
Sbjct: 535 RCCFEPSSDDYVLQTDIWQLYCSDMER 561
>SPBC14C8.17c |||SAGA complex subunit Spt8 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 526
Score = 26.6 bits (56), Expect = 2.3
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = +1
Query: 136 EQQENKKRDNPLSNIKEKFFTGKMIIIIWTLVIGLA 243
++ E ++NPL +K+K T ++ TL I +A
Sbjct: 83 KETEETPKENPLEKLKQKASTASFYDVVPTLAIPMA 118
>SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 25.8 bits (54), Expect = 4.0
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Frame = +1
Query: 202 KMIIIIWTLVIGLALLL-YLKQTYSYFSKHEIKSVTPLPILGNMGKIVF 345
K ++ L++G L+ L+ + SYF +H I S+T L + K+ F
Sbjct: 200 KKKVLTLLLLLGSRLIASILQHSNSYFDQHTISSITDERDLEDKNKLPF 248
>SPAC869.03c |||urea transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 661
Score = 25.8 bits (54), Expect = 4.0
Identities = 22/66 (33%), Positives = 31/66 (46%), Gaps = 6/66 (9%)
Frame = +1
Query: 190 FFTGKMI-IIIWTLVIGLALLLY----LKQTYSYFSKHEI-KSVTPLPILGNMGKIVFKF 351
FF G +I IIIWTL+ L + Y + T Y K I K+ P P + + + +
Sbjct: 587 FFRGWIIVIIIWTLIAALYITFYPLYESRDTIVYLCKLAIGKAKAPEP-MNYVDAVEVEI 645
Query: 352 NHLVDD 369
L DD
Sbjct: 646 ESLSDD 651
>SPAC22A12.03c |csn4||COP9/signalosome complex subunit Csn4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 377
Score = 25.8 bits (54), Expect = 4.0
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = -1
Query: 430 YKLVSTNESFFLENYCTIAKCHLL 359
YK V NE + EN C++A+C LL
Sbjct: 204 YK-VPGNELIYRENLCSVAQCLLL 226
>SPCC330.07c |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 500
Score = 25.0 bits (52), Expect = 6.9
Identities = 16/53 (30%), Positives = 27/53 (50%), Gaps = 5/53 (9%)
Frame = +1
Query: 133 SEQQENKKRDNPLSNIKEK---FFTGKMIIIIWTLVIG--LALLLYLKQTYSY 276
S+Q+EN D P + K K FF ++ I + V G ++ +Y + Y+Y
Sbjct: 293 SQQKENDDEDRPKLSWKHKFLLFFLAEVAFIAYFTVNGYQASISIYTGKLYNY 345
>SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 791
Score = 24.6 bits (51), Expect = 9.1
Identities = 14/49 (28%), Positives = 26/49 (53%), Gaps = 2/49 (4%)
Frame = +1
Query: 199 GKMIIIIWTLVIG--LALLLYLKQTYSYFSKHEIKSVTPLPILGNMGKI 339
G+ ++W ++G +++++YL Q F K K V P +LG +I
Sbjct: 649 GRYSFVMWFFLVGAVVSVVVYLLQI--SFPKSSWKHVNPALLLGGAAQI 695
>SPBC4B4.01c |||fumble family pantothenate kinase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 403
Score = 24.6 bits (51), Expect = 9.1
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = -3
Query: 386 LYNCEMSSTKWLNLKTILPMLPSIGSGVTLL 294
L CE++ LN ML +IGSGV++L
Sbjct: 187 LDTCELTFQNHLNCYHYPHMLVNIGSGVSIL 217
>SPCC16A11.03c |||DUF2009 protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 466
Score = 24.6 bits (51), Expect = 9.1
Identities = 10/36 (27%), Positives = 18/36 (50%)
Frame = +1
Query: 181 KEKFFTGKMIIIIWTLVIGLALLLYLKQTYSYFSKH 288
K +FF ++ W+ ++ LL+L+ S KH
Sbjct: 271 KTQFFYVDQSLVFWSCMMDQMFLLWLESDASLLDKH 306
>SPCC1682.03c |mug174||meiotically upregulated gene
Mug174|Schizosaccharomyces pombe|chr 3|||Manual
Length = 626
Score = 24.6 bits (51), Expect = 9.1
Identities = 13/50 (26%), Positives = 26/50 (52%)
Frame = +1
Query: 37 LNNSVITKTDKLIGEKQQRFDVHIKKRGKHL*SEQQENKKRDNPLSNIKE 186
+N+ K L E Q+ D+ + ++ ++ +KKR+ PLS+I +
Sbjct: 136 MNSKASQKQQMLSPETMQKVDLAGRASPRYQANDSWISKKRNAPLSSISQ 185
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,138,648
Number of Sequences: 5004
Number of extensions: 44526
Number of successful extensions: 140
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 140
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 214353836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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