Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt28f11
(571 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 101 1e-22
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 99 2e-22
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 87 1e-18
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 62 4e-11
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 60 2e-10
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 31 0.090
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 30 0.27
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 29 0.48
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 27 1.5
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 26 3.4
SPBC651.03c |gyp10||GTPase activating protein Gyp10|Schizosaccha... 25 7.8
SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyce... 25 7.8
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 101 bits (241), Expect = 1e-22
Identities = 52/133 (39%), Positives = 80/133 (60%)
Frame = +3
Query: 168 FQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQ 347
+++AF LFD G G+I IGD LRA GQNPT +++ + L P E + E FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLN 65
Query: 348 AISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDS 527
+ +F++G + FDKD G I ELR++L++LGEKLS++E+++LL+G
Sbjct: 66 RPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVK 125
Query: 528 QGNIXYENFVHLI 566
G + Y +FV +I
Sbjct: 126 DGMVNYHDFVQMI 138
Score = 27.5 bits (58), Expect = 1.5
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +3
Query: 165 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 278
EF + FQ+FD G I V ++ L +LG+ + ++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEM 115
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 99 bits (238), Expect = 2e-22
Identities = 54/143 (37%), Positives = 84/143 (58%), Gaps = 3/143 (2%)
Frame = +3
Query: 147 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--IS 320
+++Q+AEF+EAF LFD DG I ++G +R+LGQ+PT ++++ + D I
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 321 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVE 500
F FL + K + D + E + FDKDGNG+I+ EL H+L++LGE+LS +EV
Sbjct: 67 FTEFLTMMAR--KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVA 124
Query: 501 QLL-QGQEDSQGNIXYENFVHLI 566
++ + D G I YE F +I
Sbjct: 125 DMIREADTDGDGVINYEEFSRVI 147
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 87.4 bits (207), Expect = 1e-18
Identities = 49/141 (34%), Positives = 82/141 (58%), Gaps = 1/141 (0%)
Frame = +3
Query: 147 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFE 326
S++Q E +EAF L+D DG I + +G LR+LG N T++++ K + L + I +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL--GDAIDEK 61
Query: 327 VFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQL 506
F+ +K R ++ ++I+ R FDKD +G+I +A+ + TLGEKLSD+EV+ +
Sbjct: 62 KFMSFVS--NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLM 119
Query: 507 LQ-GQEDSQGNIXYENFVHLI 566
+Q + G+ Y +FV I
Sbjct: 120 VQEADPTNSGSFDYYDFVQRI 140
Score = 35.5 bits (78), Expect = 0.006
Identities = 16/65 (24%), Positives = 35/65 (53%)
Frame = +3
Query: 165 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 344
E+ +AF++FD G I A+ D ++ LG+ ++++V+ P SF+ + +
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Query: 345 QAISK 359
+ ++K
Sbjct: 139 RIMAK 143
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 62.5 bits (145), Expect = 4e-11
Identities = 43/143 (30%), Positives = 66/143 (46%)
Frame = +3
Query: 138 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERI 317
A + Q+ E +EAF L D GDG I + L +L Q+ +E + + P I
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINPP--I 97
Query: 318 SFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEV 497
+ FL ++ S ND +E FD +G I + +R LS++G+++ EV
Sbjct: 98 NLAAFLTAMGSMLCRIS--PRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEV 155
Query: 498 EQLLQGQEDSQGNIXYENFVHLI 566
E +L+ S G YE FV I
Sbjct: 156 ESILRSY-TSHGVFYYEKFVDAI 177
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 60.1 bits (139), Expect = 2e-10
Identities = 41/143 (28%), Positives = 66/143 (46%), Gaps = 3/143 (2%)
Frame = +3
Query: 147 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--IS 320
+E+Q + EAF+LFDS D I ++ A+RALG N +S+V K + +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 321 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVE 500
E F+ + K D + FD D G IS LR + L E + D E+E
Sbjct: 92 MEDFVRV--MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELE 149
Query: 501 QLLQGQE-DSQGNIXYENFVHLI 566
+++ + D G I + F+ ++
Sbjct: 150 AMIEEFDLDQDGEINEQEFIAIM 172
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 31.5 bits (68), Expect = 0.090
Identities = 14/55 (25%), Positives = 26/55 (47%)
Frame = +3
Query: 402 RHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNIXYENFVHLI 566
RHF+K + ++ E L++LG +E L +S+ + YE F ++
Sbjct: 494 RHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEGVTYERFTEIV 548
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 29.9 bits (64), Expect = 0.27
Identities = 16/52 (30%), Positives = 26/52 (50%)
Frame = +3
Query: 408 FDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNIXYENFVHL 563
FD G+I +LR + LGE L+ +++ QL+ + G + E F L
Sbjct: 21 FDVTHKGYIDFEDLRRSCAQLGENLTKEQL-QLMLDLAGTNGKVSREEFAEL 71
Score = 27.5 bits (58), Expect = 1.5
Identities = 18/64 (28%), Positives = 30/64 (46%)
Frame = +3
Query: 165 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 344
E +EAF LFD G I + + LG+N T+ ++ + ++S E F ++
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGKVSREEFAELW 72
Query: 345 QAIS 356
IS
Sbjct: 73 IHIS 76
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 29.1 bits (62), Expect = 0.48
Identities = 19/57 (33%), Positives = 29/57 (50%)
Frame = -3
Query: 500 LYLIITELLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVATACFRYGLVNWQK 330
L+L+ L++ +Q + R + IAIF K + +K I SVA R+ L QK
Sbjct: 1377 LHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQK 1433
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 27.5 bits (58), Expect = 1.5
Identities = 20/66 (30%), Positives = 37/66 (56%), Gaps = 4/66 (6%)
Frame = +3
Query: 324 EVFLPIYQAISKARSGDTANDFI--EGLRHFDKDGNGFISS-AELRHLLSTLGEK-LSDD 491
++F+ + I+K +G + I + RH DK GN F ++ EL +L +G+K L++
Sbjct: 643 DIFMSVTD-ITKIENGTKIYEEILQDCCRHIDKSGNEFTTAYLELLEVLLKVGQKYLAES 701
Query: 492 EVEQLL 509
+E +L
Sbjct: 702 LLEHIL 707
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 26.2 bits (55), Expect = 3.4
Identities = 16/64 (25%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Frame = -2
Query: 246 LLTHLRSERHVSFHLHEN--RRAGRLLEILPIDPLNIQPYYESTNEIEGNEIGIFKGCDL 73
+ TH+ SE L+E R ++ +LP+ P +++P G + K D+
Sbjct: 216 IFTHISSEDLAHLGLNEQYARPDWMIITVLPVPPPSVRPSISVDGTSRGEDDLTHKLSDI 275
Query: 72 SKSN 61
K+N
Sbjct: 276 IKAN 279
>SPBC651.03c |gyp10||GTPase activating protein
Gyp10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 373
Score = 25.0 bits (52), Expect = 7.8
Identities = 17/61 (27%), Positives = 29/61 (47%)
Frame = +3
Query: 381 NDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNIXYENFVH 560
N +E L H K+G+GF+ + + + +L +E L + SQ + +N VH
Sbjct: 19 NSDVETLSHIGKEGHGFLMKSLRKSVWVSLCGLSCRHRMECL--SRSTSQSSYADQNQVH 76
Query: 561 L 563
L
Sbjct: 77 L 77
>SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1131
Score = 25.0 bits (52), Expect = 7.8
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +2
Query: 425 WVHLFCGTATPALY 466
WVHL C + TP +Y
Sbjct: 927 WVHLICASWTPDVY 940
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,180,495
Number of Sequences: 5004
Number of extensions: 44158
Number of successful extensions: 182
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 242064240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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