BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt27k18
(718 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 29 0.50
SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 29 0.88
SPAC17A2.03c |vma6||V-type ATPase subunit d|Schizosaccharomyces ... 29 0.88
SPBC1105.15c |htd2||3-hydroxyacyl-ACP dehydratase Htd2 |Schizosa... 27 2.0
SPBC13G1.15c |||dubious|Schizosaccharomyces pombe|chr 2|||Manual 27 2.0
SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase... 27 3.5
SPBP8B7.28c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 27 3.5
SPBC36B7.05c |||phosphatidylinositol|Schizosaccharomyces pombe|c... 26 4.7
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi... 26 6.2
SPAC14C4.07 |||membrane transporter|Schizosaccharomyces pombe|ch... 25 8.2
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 25 8.2
>SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 504
Score = 29.5 bits (63), Expect = 0.50
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +2
Query: 230 KTLEKSNKGLSSQCSNNIYIKKEFDDTSCRICLKEGCIPIYS 355
++L+K+ GLS + + +C IC EGC+P +S
Sbjct: 107 ESLQKAGVGLSGSKQREVV----HHEGTCEICYDEGCLPFFS 144
>SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit
Bgs2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1894
Score = 28.7 bits (61), Expect = 0.88
Identities = 13/42 (30%), Positives = 24/42 (57%)
Frame = -2
Query: 129 LYKKKVLSIYYYVVCSLFLFIKQY*YLIYVNTVITRTKSYYL 4
+++ K+L YV + F+ Y + I VNTV + +S++L
Sbjct: 705 IWQPKILLGIMYVTDLVLFFLDTYLWYILVNTVFSVARSFFL 746
>SPAC17A2.03c |vma6||V-type ATPase subunit d|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 343
Score = 28.7 bits (61), Expect = 0.88
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +3
Query: 75 ERDYILHNNILIKPFFCTIKYIFLKPIQSSIQYTTWLARC 194
E++ L+ ++ F I Y FLK + I+ TW+A C
Sbjct: 288 EKEVELNKLAFLQQFHYGIVYAFLKLREQEIRNLTWIAEC 327
>SPBC1105.15c |htd2||3-hydroxyacyl-ACP dehydratase Htd2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 300
Score = 27.5 bits (58), Expect = 2.0
Identities = 21/93 (22%), Positives = 45/93 (48%), Gaps = 6/93 (6%)
Frame = +2
Query: 125 YNQIHIFKTNTKLDSIHNMVGPMLLFKMLHCKHLRKTLEKSN---KGLSSQCSNNIYIKK 295
+ +H+F+T+ H ++ + K + L KS+ K S + + ++YI++
Sbjct: 91 HRPLHLFQTS----ECHELIQEESVKSPSITKEVEACLPKSSFLTKPTSEKQTKHVYIRR 146
Query: 296 EFDDTSCRICLKEGCIPIYSNDE---IPQALSS 385
+ D+ ++C++E Y N + IP+ L S
Sbjct: 147 KIYDSQHQLCIEEDRTLAYLNRQELAIPKTLRS 179
>SPBC13G1.15c |||dubious|Schizosaccharomyces pombe|chr 2|||Manual
Length = 111
Score = 27.5 bits (58), Expect = 2.0
Identities = 12/37 (32%), Positives = 22/37 (59%), Gaps = 3/37 (8%)
Frame = -3
Query: 407 YLQICRQKSLKPVVFRR---CCRLVCNLLSNKFYMKY 306
Y+ I R + ++RR CC +C+L+ +K+Y+ Y
Sbjct: 33 YISIRRLLIVVITLYRRIVECCASLCSLIFSKYYLHY 69
>SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4
desaturase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 362
Score = 26.6 bits (56), Expect = 3.5
Identities = 10/21 (47%), Positives = 15/21 (71%)
Frame = +3
Query: 81 DYILHNNILIKPFFCTIKYIF 143
+ IL +N+L K FFCT + +F
Sbjct: 168 ELILFDNVLGKAFFCTFQLLF 188
>SPBP8B7.28c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 215
Score = 26.6 bits (56), Expect = 3.5
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = +2
Query: 314 CRICLKEGCIPIYSNDEIPQALSSFGGIFVSISDPYPKYLCQPC 445
C C K I YS + + + G VS+SD PK +C+ C
Sbjct: 42 CMRCRKVKGIDSYSKTQWSKTFTFVRGRTVSVSD--PKVICRTC 83
>SPBC36B7.05c |||phosphatidylinositol|Schizosaccharomyces pombe|chr
2|||Manual
Length = 279
Score = 26.2 bits (55), Expect = 4.7
Identities = 13/45 (28%), Positives = 22/45 (48%), Gaps = 1/45 (2%)
Frame = +2
Query: 194 LLFKMLHCKHLRKTLEKSNKGLSSQCSNNIYIKKEFDD-TSCRIC 325
L + + C + T +N+ + S C NN+ +E DD C +C
Sbjct: 129 LPYPITTCLNDDTTTRSNNQVIQSSCGNNLQRIEEPDDFVECPVC 173
>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 475
Score = 25.8 bits (54), Expect = 6.2
Identities = 14/43 (32%), Positives = 20/43 (46%)
Frame = -2
Query: 129 LYKKKVLSIYYYVVCSLFLFIKQY*YLIYVNTVITRTKSYYLP 1
LY + IY+Y + S L Y + N++ITR Y P
Sbjct: 288 LYTSVIPLIYFYSILSHLLVFAYALYSLCPNSLITRLLVQYSP 330
>SPAC14C4.07 |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 644
Score = 25.4 bits (53), Expect = 8.2
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = +3
Query: 603 SKLQRSKTKSQKITIVKNVTWN 668
S + R +T +K TIVK WN
Sbjct: 503 SSILRGRTADEKYTIVKRDVWN 524
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 25.4 bits (53), Expect = 8.2
Identities = 17/60 (28%), Positives = 29/60 (48%)
Frame = +2
Query: 281 IYIKKEFDDTSCRICLKEGCIPIYSNDEIPQALSSFGGIFVSISDPYPKYLCQPCYSLLQ 460
I + E++D ++ KE I+SN++ LS S+P+ + LC SLL+
Sbjct: 1925 IKVLLEWNDLQLKVDQKE----IFSNNQKRAILSYLFRFVCLFSEPFTEGLCSEAISLLE 1980
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,758,215
Number of Sequences: 5004
Number of extensions: 56255
Number of successful extensions: 164
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 335201398
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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